In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
Transgenic Res. 2023 Sep 14. doi: 10.1007/s11248-023-00366-6. Online ahead of print.ABSTRACTThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots....
Source: Transgenic Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Md Jakir Hossain Allah Bakhsh Faiz Ahmad Joyia Emre Aksoy Neslihan Zahide Özturk Gökçe Muhammad Sarwar Khan Source Type: research
Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
Transgenic Res. 2023 Sep 14. doi: 10.1007/s11248-023-00366-6. Online ahead of print.ABSTRACTThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots....
Source: Transgenic Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Md Jakir Hossain Allah Bakhsh Faiz Ahmad Joyia Emre Aksoy Neslihan Zahide Özturk Gökçe Muhammad Sarwar Khan Source Type: research
Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
Transgenic Res. 2023 Sep 14. doi: 10.1007/s11248-023-00366-6. Online ahead of print.ABSTRACTThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots....
Source: Transgenic Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Md Jakir Hossain Allah Bakhsh Faiz Ahmad Joyia Emre Aksoy Neslihan Zahide Özturk Gökçe Muhammad Sarwar Khan Source Type: research
Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
Transgenic Res. 2023 Sep 14. doi: 10.1007/s11248-023-00366-6. Online ahead of print.ABSTRACTThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots....
Source: Transgenic Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Md Jakir Hossain Allah Bakhsh Faiz Ahmad Joyia Emre Aksoy Neslihan Zahide Özturk Gökçe Muhammad Sarwar Khan Source Type: research
Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
Transgenic Res. 2023 Sep 14. doi: 10.1007/s11248-023-00366-6. Online ahead of print.ABSTRACTThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots....
Source: Transgenic Research - September 14, 2023 Category: Genetics & Stem Cells Authors: Md Jakir Hossain Allah Bakhsh Faiz Ahmad Joyia Emre Aksoy Neslihan Zahide Özturk Gökçe Muhammad Sarwar Khan Source Type: research
