Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00370-w. Online ahead of print.ABSTRACTCrassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicated that the gene of sweet pepper plant ferredoxin-like protein (PFLP) shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is speculated that overexpressing pflp in the transgenic plant may enhance photosynthetic efficiency through the electron tran...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Hsiang Chang Yen-Ting Chen Hsiang-En Huang Mang-Jye Ger Source Type: research
Knockout of M-LP/Mpv17L, a newly identified atypical PDE, induces physiological afferent cardiac hypertrophy in mice
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00373-7. Online ahead of print.ABSTRACTM-LP/Mpv17L (Mpv17-like protein) is an atypical cyclic nucleotide phosphodiesterase (PDE) without the molecular structure characteristic of the PDE family. Deficiency of M-LP/Mpv17L in mice has been found to result in development of β-cell hyperplasia and improved glucose tolerance. Here, we report another phenotype observed in M-LP/Mpv17L-knockout (KO) mice: afferent cardiac hypertrophy. Although the hearts of M-LP/Mpv17L-KO mice did not differ in size from those of wild-type mice, there was marked narrowing of the left ventricula...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Reiko Iida Misuzu Ueki Toshihiro Yasuda Source Type: research
Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00370-w. Online ahead of print.ABSTRACTCrassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicated that the gene of sweet pepper plant ferredoxin-like protein (PFLP) shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is speculated that overexpressing pflp in the transgenic plant may enhance photosynthetic efficiency through the electron tran...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Hsiang Chang Yen-Ting Chen Hsiang-En Huang Mang-Jye Ger Source Type: research
Knockout of M-LP/Mpv17L, a newly identified atypical PDE, induces physiological afferent cardiac hypertrophy in mice
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00373-7. Online ahead of print.ABSTRACTM-LP/Mpv17L (Mpv17-like protein) is an atypical cyclic nucleotide phosphodiesterase (PDE) without the molecular structure characteristic of the PDE family. Deficiency of M-LP/Mpv17L in mice has been found to result in development of β-cell hyperplasia and improved glucose tolerance. Here, we report another phenotype observed in M-LP/Mpv17L-knockout (KO) mice: afferent cardiac hypertrophy. Although the hearts of M-LP/Mpv17L-KO mice did not differ in size from those of wild-type mice, there was marked narrowing of the left ventricula...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Reiko Iida Misuzu Ueki Toshihiro Yasuda Source Type: research
Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00370-w. Online ahead of print.ABSTRACTCrassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicated that the gene of sweet pepper plant ferredoxin-like protein (PFLP) shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is speculated that overexpressing pflp in the transgenic plant may enhance photosynthetic efficiency through the electron tran...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Hsiang Chang Yen-Ting Chen Hsiang-En Huang Mang-Jye Ger Source Type: research
Knockout of M-LP/Mpv17L, a newly identified atypical PDE, induces physiological afferent cardiac hypertrophy in mice
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00373-7. Online ahead of print.ABSTRACTM-LP/Mpv17L (Mpv17-like protein) is an atypical cyclic nucleotide phosphodiesterase (PDE) without the molecular structure characteristic of the PDE family. Deficiency of M-LP/Mpv17L in mice has been found to result in development of β-cell hyperplasia and improved glucose tolerance. Here, we report another phenotype observed in M-LP/Mpv17L-knockout (KO) mice: afferent cardiac hypertrophy. Although the hearts of M-LP/Mpv17L-KO mice did not differ in size from those of wild-type mice, there was marked narrowing of the left ventricula...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Reiko Iida Misuzu Ueki Toshihiro Yasuda Source Type: research
Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00370-w. Online ahead of print.ABSTRACTCrassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicated that the gene of sweet pepper plant ferredoxin-like protein (PFLP) shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is speculated that overexpressing pflp in the transgenic plant may enhance photosynthetic efficiency through the electron tran...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Hsiang Chang Yen-Ting Chen Hsiang-En Huang Mang-Jye Ger Source Type: research
Knockout of M-LP/Mpv17L, a newly identified atypical PDE, induces physiological afferent cardiac hypertrophy in mice
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00373-7. Online ahead of print.ABSTRACTM-LP/Mpv17L (Mpv17-like protein) is an atypical cyclic nucleotide phosphodiesterase (PDE) without the molecular structure characteristic of the PDE family. Deficiency of M-LP/Mpv17L in mice has been found to result in development of β-cell hyperplasia and improved glucose tolerance. Here, we report another phenotype observed in M-LP/Mpv17L-knockout (KO) mice: afferent cardiac hypertrophy. Although the hearts of M-LP/Mpv17L-KO mice did not differ in size from those of wild-type mice, there was marked narrowing of the left ventricula...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Reiko Iida Misuzu Ueki Toshihiro Yasuda Source Type: research
Overexpressing plant ferredoxin-like protein enhances photosynthetic efficiency and carbohydrates accumulation in Phalaenopsis
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00370-w. Online ahead of print.ABSTRACTCrassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicated that the gene of sweet pepper plant ferredoxin-like protein (PFLP) shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is speculated that overexpressing pflp in the transgenic plant may enhance photosynthetic efficiency through the electron tran...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Hsiang Chang Yen-Ting Chen Hsiang-En Huang Mang-Jye Ger Source Type: research
Knockout of M-LP/Mpv17L, a newly identified atypical PDE, induces physiological afferent cardiac hypertrophy in mice
Transgenic Res. 2023 Oct 18. doi: 10.1007/s11248-023-00373-7. Online ahead of print.ABSTRACTM-LP/Mpv17L (Mpv17-like protein) is an atypical cyclic nucleotide phosphodiesterase (PDE) without the molecular structure characteristic of the PDE family. Deficiency of M-LP/Mpv17L in mice has been found to result in development of β-cell hyperplasia and improved glucose tolerance. Here, we report another phenotype observed in M-LP/Mpv17L-knockout (KO) mice: afferent cardiac hypertrophy. Although the hearts of M-LP/Mpv17L-KO mice did not differ in size from those of wild-type mice, there was marked narrowing of the left ventricula...
Source: Transgenic Research - October 18, 2023 Category: Genetics & Stem Cells Authors: Reiko Iida Misuzu Ueki Toshihiro Yasuda Source Type: research
Generation and validation of a myoglobin knockout zebrafish model
In conclusion, we have generated three mb knockout lines with indistinguishable phenotypes during embryonic and larval development and validated one of these lines, mb(Auzf13.2), to have no signs of genetic compensation or off-target effects in the adult heart. These findings suggests that the mb(Auzf13.2) shows promise as a candidate for investigating the biological role of Mb in zebrafish.PMID:37847464 | DOI:10.1007/s11248-023-00369-3 (Source: Transgenic Research)
Source: Transgenic Research - October 17, 2023 Category: Genetics & Stem Cells Authors: Rasmus Hejlesen Kasper Kj ær-Sørensen Angela Fago Claus Oxvig Source Type: research
Generation and validation of a myoglobin knockout zebrafish model
In conclusion, we have generated three mb knockout lines with indistinguishable phenotypes during embryonic and larval development and validated one of these lines, mb(Auzf13.2), to have no signs of genetic compensation or off-target effects in the adult heart. These findings suggests that the mb(Auzf13.2) shows promise as a candidate for investigating the biological role of Mb in zebrafish.PMID:37847464 | DOI:10.1007/s11248-023-00369-3 (Source: Transgenic Research)
Source: Transgenic Research - October 17, 2023 Category: Genetics & Stem Cells Authors: Rasmus Hejlesen Kasper Kj ær-Sørensen Angela Fago Claus Oxvig Source Type: research
A timely, user-friendly, and flexible marker-assisted speed congenics method
In this study, we present 255 microsatellites and 10 RFLPs which can be used in 18 marker panels, allowing the easy and fast introgression of genes of interest from three mouse lines commonly used for transgenesis (C57BL/6, 129/Sv and FVB) to six mouse lines relevant for biomedical research (BALB/c, C3H, DBA/1, DBA/2, SJL and SWR/J). In addition, our markers analysis confirmed a recently described lack of isogeny in well-established inbred mouse lines available from commercial breeders.PMID:37843753 | DOI:10.1007/s11248-023-00365-7 (Source: Transgenic Research)
Source: Transgenic Research - October 16, 2023 Category: Genetics & Stem Cells Authors: Anne-Sophie Van Laere Audrey Tromme Laetitia Delaval Fr édéric Farnir Jo ël Blomet Daniel Desmecht Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Transgenic Res. 2023 Sep 21. doi: 10.1007/s11248-023-00368-4. Online ahead of print.ABSTRACTGenome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigment...
Source: Transgenic Research - September 21, 2023 Category: Genetics & Stem Cells Authors: Mari Raudstein Erik Kj ærner-Semb Morten Barvik Silje Broll Anne Hege Straume Rolf Brudvik Edvardsen Source Type: research
