European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
Neurofilament Light Chain: A Translational Safety Biomarker for Drug-Induced Peripheral Neurotoxicity
This study suggests that NfL may be useful as an exploratory biomarker to assist in detecting and monitoring treatment-related peripheral nerve injury, with or without clinical signs, associated with administration of branaplam and other compounds bearing a neurotoxic risk.PMID:37439009 | DOI:10.1177/01926233231180179 (Source: Toxicologic Pathology)
Source: Toxicologic Pathology - July 13, 2023 Category: Pathology Authors: Diethilde Theil Jens Kuhle Dominique Brees Elaine Tritto Fran çois Pognan Wilfried Frieauff Kelley Penraat Emily Meseck Reginald Valdez Andreas Hartmann Source Type: research
Neurofilament Light Chain: A Translational Safety Biomarker for Drug-Induced Peripheral Neurotoxicity
This study suggests that NfL may be useful as an exploratory biomarker to assist in detecting and monitoring treatment-related peripheral nerve injury, with or without clinical signs, associated with administration of branaplam and other compounds bearing a neurotoxic risk.PMID:37439009 | DOI:10.1177/01926233231180179 (Source: Toxicologic Pathology)
Source: Toxicologic Pathology - July 13, 2023 Category: Pathology Authors: Diethilde Theil Jens Kuhle Dominique Brees Elaine Tritto Fran çois Pognan Wilfried Frieauff Kelley Penraat Emily Meseck Reginald Valdez Andreas Hartmann Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Retinal and Systemic Toxicity of Vigabatrin Is Driven by the S-Enantiomer in the Long Evans Rat
In this study, we assessed the toxicity and toxicokinetics of racemic vigabatrin and its S- and R-enantiomers (vigabatrin consists of 50:50% of the two enantiomers) by administering doses of the three test articles to male Long Evans rats via oral gavage. The animals were housed under high-intensity light conditions and the study consisted of an escalating dose phase and a 21-day fixed-dose phase. Systemic toxicity of vigabatrin appears to be due to the Vig-S-enantiomer only, as increasing doses of Vig-S or Vig-RS caused body weight loss, decreased food consumption, and affected activity. Administration of the Vig-R-enanti...
Source: Toxicologic Pathology - July 4, 2023 Category: Pathology Authors: Allan D Rasmussen Nathalie Truchot Agnete Dyssegaard Pierluigi Fant Source Type: research
Corrigendum to Histology Atlas of the Developing Mouse Placenta
Toxicol Pathol. 2023 May 9:1926233231175051. doi: 10.1177/01926233231175051. Online ahead of print.NO ABSTRACTPMID:37160757 | DOI:10.1177/01926233231175051 (Source: Toxicologic Pathology)
Source: Toxicologic Pathology - May 10, 2023 Category: Pathology Source Type: research
