Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
Scientific and Regulatory Policy Committee Points to Consider: Sampling, Processing, Evaluation, Interpretation, and Reporting of Test Article-Related Ganglion Pathology for Nonclinical Toxicity Studies
This article outlines "points to consider" for sample collection, processing, evaluation, interpretation, and reporting of ganglion findings; these points are consistent with published best practices for peripheral nervous system evaluation in nonclinical toxicity studies. Ganglion findings often occur as a combination of neuronal injury (e.g., degeneration, necrosis, and/or loss) and/or glial effects (e.g., increased satellite glial cell cellularity) with leukocyte accumulation (e.g., mononuclear cell infiltration or inflammation). Nerve fiber degeneration and/or glial reactions may be seen in nerves, dorsal spinal nerve ...
Source: Toxicologic Pathology - July 25, 2023 Category: Pathology Authors: Bindu M Bennet Ingrid D Pardo Basel T Assaf Elizabeth Buza Sarah Cramer LaTasha K Crawford Jeffery A Engelhardt Branka Grubor James P Morrison Tanasa S Osborne Alok K Sharma Brad Bolon Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
Toxicol Pathol. 2023 Jul 14:1926233231178282. doi: 10.1177/01926233231178282. Online ahead of print.ABSTRACTIn situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increas...
Source: Toxicologic Pathology - July 14, 2023 Category: Pathology Authors: Josep M Monn é Rodríguez Anna-Lena Frisk Robert Kreutzer Thomas Lemarchand Stephane Lezmi Chandrassegar Saravanan Birgit Stierstorfer C éline Thuilliez Enrico Vezzali Grazyna Wieczorek Seong-Wook Yun Dirk Schaudien Source Type: research
