In vivo treatment with calcilytic of CaSR knock-in mice ameliorates renal phenotype reversing downregulation of the vasopressin-AQP2 pathway
J Physiol. 2024 Feb 17. doi: 10.1113/JP284233. Online ahead of print.ABSTRACTHigh concentrations of urinary calcium counteract vasopressin action via the activation of the Calcium-Sensing Receptor (CaSR) expressed in the luminal membrane of the collecting duct cells, which impairs the trafficking of aquaporin-2 (AQP2). In line with these findings, we provide evidence that, with respect to wild-type mice, CaSR knock-in (KI) mice mimicking autosomal dominant hypocalcaemia, display a significant decrease in the total content of AQP2 associated with significantly higher levels of AQP2 phosphorylation at Ser261, a phosphorylati...
Source: The Journal of Physiology - February 17, 2024 Category: Physiology Authors: Marianna Ranieri Ines Angelini Mariagrazia D'Agostino Annarita Di Mise Mariangela Centrone Maria Venneri Angela Ferrulli Maria Mastrodonato Grazia Tamma Itsuro Endo Seiji Fukumoto Toshio Matsumoto Giovanna Valenti Source Type: research
Hormonal intrauterine devices and heat exchange during exercise
J Physiol. 2024 Feb 17. doi: 10.1113/JP285977. Online ahead of print.ABSTRACTSynthetic progestins in oral contraceptives are thought to blunt heat dissipation by reducing skin blood flow and sweating. However, whether progestin-releasing intrauterine devices (IUDs) modulate heat loss during exercise-heat stress is unknown. We used direct calorimetry to measure whole-body total (dry + evaporative) heat loss in young, physically active women (mean (SD); aged 24 (4) years, V ̇ O 2 peak ${\dot V_{{{\mathrm{O}}_{\mathrm{2}}}{\mathrm{peak}}}}$ 39.3 (5.3) ml/kg/min) with (IUD; n = 19) and without (Control; n = 17) IUDs in th...
Source: The Journal of Physiology - February 17, 2024 Category: Physiology Authors: Nathalie V Kirby Robert D Meade Brodie J Richards Sean R Notley Glen P Kenny Source Type: research
SGLT2 inhibitors improve cardiac function by improving intracellular calcium availability and optimizing the cell shape of cardiac myocytes
J Physiol. 2024 Feb 17. doi: 10.1113/JP286219. Online ahead of print.NO ABSTRACTPMID:38367225 | DOI:10.1113/JP286219 (Source: The Journal of Physiology)
Source: The Journal of Physiology - February 17, 2024 Category: Physiology Authors: Sven Kurbel Katarina Dodig- Ćurković Source Type: research
In vivo treatment with calcilytic of CaSR knock-in mice ameliorates renal phenotype reversing downregulation of the vasopressin-AQP2 pathway
J Physiol. 2024 Feb 17. doi: 10.1113/JP284233. Online ahead of print.ABSTRACTHigh concentrations of urinary calcium counteract vasopressin action via the activation of the Calcium-Sensing Receptor (CaSR) expressed in the luminal membrane of the collecting duct cells, which impairs the trafficking of aquaporin-2 (AQP2). In line with these findings, we provide evidence that, with respect to wild-type mice, CaSR knock-in (KI) mice mimicking autosomal dominant hypocalcaemia, display a significant decrease in the total content of AQP2 associated with significantly higher levels of AQP2 phosphorylation at Ser261, a phosphorylati...
Source: The Journal of Physiology - February 17, 2024 Category: Physiology Authors: Marianna Ranieri Ines Angelini Mariagrazia D'Agostino Annarita Di Mise Mariangela Centrone Maria Venneri Angela Ferrulli Maria Mastrodonato Grazia Tamma Itsuro Endo Seiji Fukumoto Toshio Matsumoto Giovanna Valenti Source Type: research
Hormonal intrauterine devices and heat exchange during exercise
J Physiol. 2024 Feb 17. doi: 10.1113/JP285977. Online ahead of print.ABSTRACTSynthetic progestins in oral contraceptives are thought to blunt heat dissipation by reducing skin blood flow and sweating. However, whether progestin-releasing intrauterine devices (IUDs) modulate heat loss during exercise-heat stress is unknown. We used direct calorimetry to measure whole-body total (dry + evaporative) heat loss in young, physically active women (mean (SD); aged 24 (4) years, V ̇ O 2 peak ${\dot V_{{{\mathrm{O}}_{\mathrm{2}}}{\mathrm{peak}}}}$ 39.3 (5.3) ml/kg/min) with (IUD; n = 19) and without (Control; n = 17) IUDs in th...
Source: The Journal of Physiology - February 17, 2024 Category: Physiology Authors: Nathalie V Kirby Robert D Meade Brodie J Richards Sean R Notley Glen P Kenny Source Type: research
Personalized depth-specific neuromodulation of the human primary motor cortex via ultrasound
J Physiol. 2024 Feb 15. doi: 10.1113/JP285613. Online ahead of print.ABSTRACTNon-invasive brain stimulation has the potential to boost neuronal plasticity in the primary motor cortex (M1), but it remains unclear whether the stimulation of both superficial and deep layers of the human motor cortex can effectively promote M1 plasticity. Here, we leveraged transcranial ultrasound stimulation (TUS) to precisely target M1 circuits at depths of approximately 5 mm and 16 mm from the cortical surface. Initially, we generated computed tomography images from each participant's individual anatomical magnetic resonance images (MRI), w...
Source: The Journal of Physiology - February 15, 2024 Category: Physiology Authors: Shancheng Bao Hakjoo Kim Nandan B Shettigar Yue Li Yuming Lei Source Type: research
Rise and shine, it's exercising to improve metabolic syndrome time
J Physiol. 2024 Feb 15. doi: 10.1113/JP286068. Online ahead of print.NO ABSTRACTPMID:38358854 | DOI:10.1113/JP286068 (Source: The Journal of Physiology)
Source: The Journal of Physiology - February 15, 2024 Category: Physiology Authors: CeAnn C Udovich Source Type: research
Personalized depth-specific neuromodulation of the human primary motor cortex via ultrasound
J Physiol. 2024 Feb 15. doi: 10.1113/JP285613. Online ahead of print.ABSTRACTNon-invasive brain stimulation has the potential to boost neuronal plasticity in the primary motor cortex (M1), but it remains unclear whether the stimulation of both superficial and deep layers of the human motor cortex can effectively promote M1 plasticity. Here, we leveraged transcranial ultrasound stimulation (TUS) to precisely target M1 circuits at depths of approximately 5 mm and 16 mm from the cortical surface. Initially, we generated computed tomography images from each participant's individual anatomical magnetic resonance images (MRI), w...
Source: The Journal of Physiology - February 15, 2024 Category: Physiology Authors: Shancheng Bao Hakjoo Kim Nandan B Shettigar Yue Li Yuming Lei Source Type: research
Rise and shine, it's exercising to improve metabolic syndrome time
J Physiol. 2024 Feb 15. doi: 10.1113/JP286068. Online ahead of print.NO ABSTRACTPMID:38358854 | DOI:10.1113/JP286068 (Source: The Journal of Physiology)
Source: The Journal of Physiology - February 15, 2024 Category: Physiology Authors: CeAnn C Udovich Source Type: research
Personalized depth-specific neuromodulation of the human primary motor cortex via ultrasound
J Physiol. 2024 Feb 15. doi: 10.1113/JP285613. Online ahead of print.ABSTRACTNon-invasive brain stimulation has the potential to boost neuronal plasticity in the primary motor cortex (M1), but it remains unclear whether the stimulation of both superficial and deep layers of the human motor cortex can effectively promote M1 plasticity. Here, we leveraged transcranial ultrasound stimulation (TUS) to precisely target M1 circuits at depths of approximately 5 mm and 16 mm from the cortical surface. Initially, we generated computed tomography images from each participant's individual anatomical magnetic resonance images (MRI), w...
Source: The Journal of Physiology - February 15, 2024 Category: Physiology Authors: Shancheng Bao Hakjoo Kim Nandan B Shettigar Yue Li Yuming Lei Source Type: research
Rise and shine, it's exercising to improve metabolic syndrome time
J Physiol. 2024 Feb 15. doi: 10.1113/JP286068. Online ahead of print.NO ABSTRACTPMID:38358854 | DOI:10.1113/JP286068 (Source: The Journal of Physiology)
Source: The Journal of Physiology - February 15, 2024 Category: Physiology Authors: CeAnn C Udovich Source Type: research
Circulating noradrenaline leads to release of neuropeptide Y from cardiac sympathetic nerve terminals via activation of β-adrenergic receptors
In this study, fast-scan cyclic voltammetry and capacitive immunoprobes were used to allow for real-time in vivo measurements of interstitial myocardial neurotransmitters and neuropeptides, respectively. Using an in vivo porcine model with cardiac fast-scan cyclic voltammetry and capacitive immunoprobes, it was shown that systemic NA can increase ventricular interstitial NPY levels, suggesting that NA induces NPY release from postganglionic sympathetic nerves. The release of NPY was blocked by administration of the non-selective β-blocker propranolol, suggesting that release of NPY is dependent on activation of β-adrener...
Source: The Journal of Physiology - February 14, 2024 Category: Physiology Authors: Valerie Y H van Weperen Jonathan D Hoang Neil R Jani Artin Khaky Neil Herring Corey Smith Marmar Vaseghi Source Type: research
Inspiratory and sigh breathing rhythms depend on distinct cellular signalling mechanisms in the preB ötzinger complex
J Physiol. 2024 Feb 14. doi: 10.1113/JP285582. Online ahead of print.ABSTRACTBreathing behaviour involves the generation of normal breaths (eupnoea) on a timescale of seconds and sigh breaths on the order of minutes. Both rhythms emerge in tandem from a single brainstem site, but whether and how a single cell population can generate two disparate rhythms remains unclear. We posit that recurrent synaptic excitation in concert with synaptic depression and cellular refractoriness gives rise to the eupnoea rhythm, whereas an intracellular calcium oscillation that is slower by orders of magnitude gives rise to the sigh rhythm. ...
Source: The Journal of Physiology - February 14, 2024 Category: Physiology Authors: Daniel S Borrus Marco K Stettler Cameron J Grover Eva J Kalajian Jeffrey Gu Gregory D Conradi Smith Christopher A Del Negro Source Type: research
Cortical-brainstem circuitry attenuates physiological stress reactivity
J Physiol. 2024 Feb 14. doi: 10.1113/JP285627. Online ahead of print.ABSTRACTExposure to stressful stimuli promotes multi-system biological responses to restore homeostasis. Catecholaminergic neurons in the rostral ventrolateral medulla (RVLM) facilitate sympathetic activity and promote physiological adaptations, including glycaemic mobilization and corticosterone release. While it is unclear how brain regions involved in the cognitive appraisal of stress regulate RVLM neural activity, recent studies found that the rodent ventromedial prefrontal cortex (vmPFC) mediates stress appraisal and physiological stress responses. T...
Source: The Journal of Physiology - February 14, 2024 Category: Physiology Authors: Sebastian A Pace Ema Lukinic Tyler Wallace Carlie McCartney Brent Myers Source Type: research
Severe hypoxaemic hypercapnia compounds cerebral oxidative-nitrosative stress during extreme apnoea: Implications for cerebral bioenergetic function
We examined the extent to which apnoea-induced extremes of oxygen demand/carbon dioxide production impact redox regulation of cerebral bioenergetic function. Ten ultra-elite apnoeists (six men and four women) performed two maximal dry apnoeas preceded by normoxic normoventilation, resulting in severe end-apnoea hypoxaemic hypercapnia, and hyperoxic hyperventilation designed to ablate hypoxaemia, resulting in hyperoxaemic hypercapnia. Transcerebral exchange of ascorbate radicals (by electron paramagnetic resonance spectroscopy) and nitric oxide metabolites (by tri-iodide chemiluminescence) were calculated as the product of ...
Source: The Journal of Physiology - February 13, 2024 Category: Physiology Authors: Damian M Bailey Anthony R Bain Ryan L Hoiland Otto F Barak Ivan Drvis Benjamin S Stacey Angelo Iannetelli Gareth W Davison Rasmus H Dahl Ronan M G Berg David B MacLeod Zeljko Dujic Philip N Ainslie Source Type: research
