Artificial oocyte activation: physiological, pathophysiological and ethical aspects
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 12, 2018 Category: Sexual Medicine Authors: George Anifandis Alexandros Michopoulos Alexandros Daponte Katerina Chatzimeletiou Mara Simopoulou Christina I. Messini Nikolas P. Polyzos Katerina Vassiou Konstantinos Dafopoulos Dimitrios G. Goulis Source Type: research

Artificial oocyte activation: physiological, pathophysiological and ethical aspects.
In conclusion, in mammalian eggs, the cytosolic Ca+2 oscillations derive from a periodic release of Ca+2 from intracellular pools. PLCζ, as well as artificial stimulants, have been used to produce Ca+2 oscillations for AOA. As the latter may increase the risk of epigenetic induced malformations, further studies are required to clarify whether AOA constitutes an effective and safe method to overcome ICSI failure. ABBREVIATIONS: AOA: artificial oocyte activation; AOD: oocyte activation deficiency; Ca+2: Calcium; CAMKII: Ca+2/calmodulin-dependent protein kinase II; CICR: calcium-induced calcium-release; DAG: diacylg...
Source: Systems Biology in Reproductive Medicine - September 12, 2018 Category: Reproduction Medicine Authors: Anifandis G, Michopoulos A, Daponte A, Chatzimeletiou K, Simopoulou M, Messini CI, Polyzos NP, Vassiou K, Dafopoulos K, Goulis DG Tags: Syst Biol Reprod Med Source Type: research

Artificial oocyte activation: physiological, pathophysiological and ethical aspects
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 12, 2018 Category: Sexual Medicine Authors: George Anifandis Alexandros Michopoulos Alexandros Daponte Katerina Chatzimeletiou Mara Simopoulou Christina I. Messini Nikolas P. Polyzos Katerina Vassiou Konstantinos Dafopoulos Dimitrios G. Goulis Source Type: research

Research advances in the regulation of the putative ovarian germline stem cell niche on female germline stem cells
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 11, 2018 Category: Sexual Medicine Authors: Yangchun Liu Jiao Xu Feiyin Zhu Haifeng Ye Chuan Hu Jian Huang Yuehui Zheng Source Type: research

Research advances in the regulation of the putative ovarian germline stem cell niche on female germline stem cells.
Abstract Stem cells are ideal seeding cells, which have the potential for self-renewal and multiple differentiation, and they play a fundamental role in maintaining homeostasis and regenerating and repairing tissue. The discovery of female germline stem cells (FGSCs) brings much hope for the postnatal renewal of oocytes and solving some female infertility problems. Ovarian function declines with increasing female age. Moreover, ovarian germline stem cell niche-aging could be the main cause of ovarian senescence, which ultimately leads to decreased follicle generation, declining female fertility, and age-related di...
Source: Systems Biology in Reproductive Medicine - September 11, 2018 Category: Reproduction Medicine Authors: Liu Y, Xu J, Zhu F, Ye H, Hu C, Huang J, Zheng Y Tags: Syst Biol Reprod Med Source Type: research

Research advances in the regulation of the putative ovarian germline stem cell niche on female germline stem cells
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 11, 2018 Category: Sexual Medicine Authors: Yangchun Liu Jiao Xu Feiyin Zhu Haifeng Ye Chuan Hu Jian Huang Yuehui Zheng Source Type: research

Protein evolution revisited
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 4, 2018 Category: Sexual Medicine Authors: Peter L. Davies Laurie A. Graham Source Type: research

Protein evolution revisited.
Abstract Antifreeze proteins (AFPs) protect marine fishes from freezing in icy seawater. They evolved relatively recently, most likely in response to the formation of sea ice and Cenozoic glaciations that occurred less than 50 million years ago, following a greenhouse Earth event. Based on their diversity, AFPs have independently evolved on many occasions to serve the same function, with some remarkable examples of convergent evolution at the structural level, and even instances of lateral gene transfer. For some AFPs, the progenitor gene is recognizable. The intense selection pressure exerted by icy seawater, whi...
Source: Systems Biology in Reproductive Medicine - September 4, 2018 Category: Reproduction Medicine Authors: Davies PL, Graham LA Tags: Syst Biol Reprod Med Source Type: research

Protein evolution revisited
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 4, 2018 Category: Sexual Medicine Authors: Peter L. Davies Laurie A. Graham Source Type: research

New monoclonal antibodies specific for mammalian protamines P1 and P2
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 31, 2018 Category: Sexual Medicine Authors: Rod Balhorn Klaus Steger Martin Bergmann Hans-Christian Schuppe Stefanie Neuhauser Monique C. Balhorn Source Type: research

New monoclonal antibodies specific for mammalian protamines P1 and P2.
Abstract The expression of protamines and the binding of these small arginine-rich proteins to DNA complete the process of spermatid chromatin reorganization and the global inactivation of the male's haploid genome that occurs during the final stages of sperm development in mammals. While a number of anti-protamine antibodies have been created during the last 40 years, only a few have proven useful for detecting the presence of the protamines, determining the timing of their expression and deposition in chromatin, and investigating their structure and function in both maturing spermatids and sperm. The aim of...
Source: Systems Biology in Reproductive Medicine - August 31, 2018 Category: Reproduction Medicine Authors: Balhorn R, Steger K, Bergmann M, Schuppe HC, Neuhauser S, Balhorn MC Tags: Syst Biol Reprod Med Source Type: research

Gordon H. Dixon ’s trace in my personal career and the quantic jump experienced in regulatory information
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 23, 2018 Category: Sexual Medicine Authors: Jovita Mezquita-Pla Source Type: research

Antisense techniques provide robust decrease in GnRH receptor expression with minimal cytotoxicity in GT1-7 cells*
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 23, 2018 Category: Sexual Medicine Authors: Maurice Andre Recanati Hongling Du Katherine J. Kramer Maik H üttemann Robert A. Welch Source Type: research

Antisense techniques provide robust decrease in GnRH receptor expression with minimal cytotoxicity in GT1-7 cells.
This study shows methods and optimized conditions established for the generation of a stable GT1-7 cell line containing an inducible construct allowing the downregulation of GnRH receptor expression. ABBREVIATIONS: ANOVA: analysis of the variance; DMEM: Dulbecco's modified Eagle's medium; GnRH: gonadotropin-releasing hormone; RXR: retinoid X receptor. PMID: 30136857 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 23, 2018 Category: Reproduction Medicine Authors: Recanati MA, Du H, Kramer KJ, Hüttemann M, Welch RA Tags: Syst Biol Reprod Med Source Type: research

Gordon H. Dixon's trace in my personal career and the quantic jump experienced in regulatory information.
Abstract Even before Rosalin Franklin had discovered the DNA double helix, in her impressive X-ray diffraction image pattern, Erwin Schröedinger, described, in his excellent book, What is Life, how the finding of aperiodic crystals in biological systems surprised him (an aperiodic crystal, which, in my opinion is the material carrier of life). In the 21st century and still far from being able to define life, we are attending to a quick acceleration of knowledge on regulatory information. With the discovery of new codes and punctuation marks, we will greatly increase our understanding in front of an impressive...
Source: Systems Biology in Reproductive Medicine - August 23, 2018 Category: Reproduction Medicine Authors: Mezquita-Pla J Tags: Syst Biol Reprod Med Source Type: research

Gordon H. Dixon ’s trace in my personal career and the quantic jump experienced in regulatory information
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 23, 2018 Category: Sexual Medicine Authors: Jovita Mezquita-Pla Source Type: research

Antisense techniques provide robust decrease in GnRH receptor expression with minimal cytotoxicity in GT1-7 cells*
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 23, 2018 Category: Sexual Medicine Authors: Maurice Andre Recanati Hongling Du Katherine J. Kramer Maik H üttemann Robert A. Welch Source Type: research

Gordon Dixon, protamines, and the atypical patterns of gene expression in spermatogenic cells
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 21, 2018 Category: Sexual Medicine Authors: Kenneth Cole Kleene Source Type: research

Gordon Dixon, protamines, and the atypical patterns of gene expression in spermatogenic cells.
Abstract Gordon Dixon's pioneering work on the replacement of histones by protamines during spermatogenesis inspired research as recombinant DNA became widely used to analyze gene expression in mammalian spermatogenic cells. The impact of recombinant DNA began immediately with the identification of mouse protamine 1 as a haploid-expressed mRNA, resolving a decades-long controversy whether gene expression in haploid spermatogenic cells distorts transmission of alleles to progeny. Numerous insights into the biology of spermatogenesis followed as the sequences of many mRNAs revealed that the patterns of gene expressi...
Source: Systems Biology in Reproductive Medicine - August 21, 2018 Category: Reproduction Medicine Authors: Kleene KC Tags: Syst Biol Reprod Med Source Type: research

Gordon Dixon, protamines, and the atypical patterns of gene expression in spermatogenic cells
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 21, 2018 Category: Sexual Medicine Authors: Kenneth Cole Kleene Source Type: research

Effects of endometrial preparations and transferred embryo types on pregnancy outcome from patients with advanced maternal age
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 9, 2018 Category: Sexual Medicine Authors: Jie Liu Jie Zheng Ya-lan Lei Xiao-feng Wen Source Type: research

Effects of endometrial preparations and transferred embryo types on pregnancy outcome from patients with advanced maternal age.
Abstract We explored the strategy of frozen-thawed embryo transfer (FET) in the women with advanced maternal age (AMA). We first determined the age cut-off point of AMA by retrospective analysis of pregnancy outcomes in the patients undergoing FET. The patients with AMA were divided into 3 groups including natural cycle (NC) group, controlled ovarian stimulation (COS) group, and hormone replacement therapy (HRT) group, and simultaneously were divided into 2 groups including cleavage-stage embryo transfer (CET) group and blastocyst-stage embryo transfer (BET) group. The clinical pregnancy, embryo implantation, abor...
Source: Systems Biology in Reproductive Medicine - August 9, 2018 Category: Reproduction Medicine Authors: Liu J, Zheng J, Lei YL, Wen XF Tags: Syst Biol Reprod Med Source Type: research

Effects of endometrial preparations and transferred embryo types on pregnancy outcome from patients with advanced maternal age
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 9, 2018 Category: Sexual Medicine Authors: Jie Liu Jie Zheng Ya-lan Lei Xiao-feng Wen Source Type: research

Making IVF more effective through the evolution of prediction models: is prognosis the missing piece of the puzzle?
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 8, 2018 Category: Sexual Medicine Authors: Mara Simopoulou Konstantinos Sfakianoudis Nikolaos Antoniou Evangelos Maziotis Anna Rapani Panagiotis Bakas George Anifandis Theodoros Kalampokas Stamatis Bolaris Agni Pantou Konstantinos Pantos Michael Koutsilieris Source Type: research

Making IVF more effective through the evolution of prediction models: is prognosis the missing piece of the puzzle?
Abstract Assisted reproductive technology has evolved tremendously since the emergence of in vitro fertilization (IVF). In the course of the recent decade, there have been significant efforts in order to minimize multiple gestations, while improving percentages of singleton pregnancies and offering individualized services in IVF, in line with the trend of personalized medicine. Patients as well as clinicians and the entire IVF team benefit majorly from 'knowing what to expect' from an IVF cycle. Hereby, the question that has emerged is to what extent prognosis could facilitate toward the achievement of the above g...
Source: Systems Biology in Reproductive Medicine - August 8, 2018 Category: Reproduction Medicine Authors: Simopoulou M, Sfakianoudis K, Antoniou N, Maziotis E, Rapani A, Bakas P, Anifandis G, Kalampokas T, Bolaris S, Pantou A, Pantos K, Koutsilieris M Tags: Syst Biol Reprod Med Source Type: research

Making IVF more effective through the evolution of prediction models: is prognosis the missing piece of the puzzle?
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 8, 2018 Category: Sexual Medicine Authors: Mara Simopoulou Konstantinos Sfakianoudis Nikolaos Antoniou Evangelos Maziotis Anna Rapani Panagiotis Bakas George Anifandis Theodoros Kalampokas Stamatis Bolaris Agni Pantou Konstantinos Pantos Michael Koutsilieris Source Type: research

Lifestyle and demographic factors associated with human semen quality and sperm function
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 23, 2018 Category: Sexual Medicine Authors: Xiao Shi Carol Pui Shan Chan Tarah Waters Ling Chi David Yiu Leung Chan Tin-Chiu Li Source Type: research

Lifestyle and demographic factors associated with human semen quality and sperm function.
In this study, we sought to investigate the effect of lifestyle and demographic factors on classic and functional semen parameters. Three hundred and twenty-eight subjects who underwent semen analysis were recruited. Routine SA, sperm vitality, acrosome reaction (AR) assay and sperm DNA fragmentation index (DFI) were analyzed. Demographic and lifestyle information, including (1) BMI, (2) current smoking and alcohol drinking frequency, (3) sleep habits, (4) daily fluid intake, (5) weekly meat intake, (6) sports frequency, (7) trouser cell phone use, (8) age, and (9) abstinence time, were collected. Generalized additive mode...
Source: Systems Biology in Reproductive Medicine - July 23, 2018 Category: Reproduction Medicine Authors: Shi X, Chan CPS, Waters T, Chi L, Chan DYL, Li TC Tags: Syst Biol Reprod Med Source Type: research

Lifestyle and demographic factors associated with human semen quality and sperm function
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 23, 2018 Category: Sexual Medicine Authors: Xiao Shi Carol Pui Shan Chan Tarah Waters Ling Chi David Yiu Leung Chan Tin-Chiu Li Source Type: research

Comparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 9, 2018 Category: Sexual Medicine Authors: Soraya Labied Caroline Jouan Fr édéric Wenders St éphanie Ravet Olivier Gaspard Fabienne Thonon Virginie Gridelet Laurie Henry Sophie Perrier d ’Hauterive Michelle Nisolle Source Type: research

Comparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study.
Abstract The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil™, a paraffin oil, and LiteOil®, a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by  intracytoplasmic sperm injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randoml...
Source: Systems Biology in Reproductive Medicine - July 9, 2018 Category: Reproduction Medicine Authors: Labied S, Jouan C, Wenders F, Ravet S, Gaspard O, Thonon F, Gridelet V, Henry L, Perrier d'Hauterive S, Nisolle M Tags: Syst Biol Reprod Med Source Type: research

Comparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 9, 2018 Category: Sexual Medicine Authors: Soraya Labied Caroline Jouan Fr édéric Wenders St éphanie Ravet Olivier Gaspard Fabienne Thonon Virginie Gridelet Laurie Henry Sophie Perrier d ’Hauterive Michelle Nisolle Source Type: research

Is growth hormone administration essential for in vitro fertilization treatment of female patients with growth hormone deficiency?
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 5, 2018 Category: Sexual Medicine Authors: Drago ş Albu Alice Albu Source Type: research

Is growth hormone administration essential for in vitro fertilization treatment of female patients with growth hormone deficiency?
In conclusion, our case report suggests that adequate levels of growth hormone are essential for an adequate competence of the oocytes in infertile patients with growth hormone deficiency. Therefore, growth hormone administration should be taken into consideration for patients with this deficiency in order to optimize the results of infertility treatment. ABBREVIATIONS: GH: growth hormone; GHD: growth hormone deficiency; HCG: human chorionic gonadotropin; IVF: in vitro fertilization; IGF: insulin like growth factor. PMID: 29975109 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 5, 2018 Category: Reproduction Medicine Authors: Albu D, Albu A Tags: Syst Biol Reprod Med Source Type: research

Application of metabolomics to preeclampsia diagnosis
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 2, 2018 Category: Sexual Medicine Authors: B. Fatemeh Nobakht M. Gh Source Type: research

Application of metabolomics to preeclampsia diagnosis.
Abstract Preeclampsia is a multifactorial disorder defined by hypertension and increased urinary protein excretion during pregnancy. It is a significant cause of maternal and neonatal deaths worldwide. Despite various research efforts to clarify pathogenies of preeclampsia and predict this disease before beginning of symptoms, the pathogenesis of preeclampsia is unclear. Early prediction and diagnosis of women at risk of preeclampsia has not markedly improved. Therefore, the objective of this study was to perform a review on metabolomic articles assessing predictive and diagnostic biomarkers of preeclampsia. Four ...
Source: Systems Biology in Reproductive Medicine - July 2, 2018 Category: Reproduction Medicine Authors: Nobakht M Gh BF Tags: Syst Biol Reprod Med Source Type: research

Successful application of a single warming protocol for embryos cryopreserved by either slow freezing or vitrification techniques
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 28, 2018 Category: Sexual Medicine Authors: Munevver Serdarogullari Onder Coban Fazilet Kubra Boynukalin Ekrem Murat Bilgin Necati Findikli Mustafa Bahceci Source Type: research

Successful application of a single warming protocol for embryos cryopreserved by either slow freezing or vitrification techniques.
Abstract The aim of this study was to evaluate the feasibility and efficiency of using a sucrose gradient-based warming protocol as a universal warming approach on human cleavage stage embryos. Between January 2013 and November 2014, a total of 118 warming cycles were performed on 705 embryos which had previously been cryopreserved/thawed by slow freezing protocols or cryopreserved by slow freezing and warmed by vitrification thaw solution. Clinical outcomes have been retrospectively analyzed depending on cryopreservation and warming techniques used, embryo viability, day of cryopreservation, clinical pregnancy, i...
Source: Systems Biology in Reproductive Medicine - June 28, 2018 Category: Reproduction Medicine Authors: Serdarogullari M, Coban O, Boynukalin FK, Bilgin EM, Findikli N, Bahceci M Tags: Syst Biol Reprod Med Source Type: research

Identification of a complex population of chromatin-associated proteins in the European sea bass (Dicentrarchus labrax) sperm
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 25, 2018 Category: Sexual Medicine Authors: Ferran Barrachina Dafni Anastasiadi Meritxell Jodar Judit Castillo Josep Maria Estanyol Francesc Piferrer Rafael Oliva Source Type: research

Identification of a complex population of chromatin-associated proteins in the European sea bass (Dicentrarchus labrax) sperm.
Abstract A very common conception about the function of the spermatozoon is that its unique role is to transmit the paternal genome to the next generation. Most of the sperm genome is known to be condensed in many species by protamines, which are small and extremely positively charged proteins (50-70% arginine) with the functions of streamlining the sperm cell and protecting its DNA. However, more recently, it has been shown in mammals that 2-10% of its mature sperm chromatin is also associated to a complex population of histones and chromatin-associated proteins differentially distributed in the genome. These pro...
Source: Systems Biology in Reproductive Medicine - June 25, 2018 Category: Reproduction Medicine Authors: Barrachina F, Anastasiadi D, Jodar M, Castillo J, Estanyol JM, Piferrer F, Oliva R Tags: Syst Biol Reprod Med Source Type: research

Maternal reproductive performance and fetal development of the Wistar Audiogenic Rat (WAR) strain
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 21, 2018 Category: Sexual Medicine Authors: Eduardo H. Umeoka Matheus C. Eiras Iara G. Viana Vanessa S. Giorgi Aline Bueno D ébora C. Damasceno Norberto Garcia-Cairasco Paula A. Navarro Source Type: research

Maternal reproductive performance and fetal development of the Wistar Audiogenic Rat (WAR) strain.
Abstract Wistar Audiogenic Rat (WAR) strain is an animal model for epilepsy studies, the chronic multifactorial disease that affects millions of people worldwide. The animals of this strain are genetically predisposed to sound-induced seizures, called audiogenic seizures, and have been used for many years in studies to understand the mechanisms involved in the epilepsies and their neuropsychiatric comorbidities, as well as the screening of potential anti-convulsant agents. Nevertheless, little is known about the reproductive characteristics of these animals. The main goal of this study was to characterize the fema...
Source: Systems Biology in Reproductive Medicine - June 21, 2018 Category: Reproduction Medicine Authors: Umeoka EH, Eiras MC, Viana IG, Giorgi VS, Bueno A, Damasceno DC, Garcia-Cairasco N, Navarro PA Tags: Syst Biol Reprod Med Source Type: research

Association of estrogen, progesterone and follicle stimulating hormone receptor polymorphisms with in vitro fertilization outcomes
Volume 64, Issue 4, August 2018, Page 260-265 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 19, 2018 Category: Sexual Medicine Authors: Vijaya Ganesh Vettriselvi Venkatesan Teena Koshy Sanjeeva Nellapalli Reddy Suruli Muthumuthiah Solomon Franklin Durairaj Paul Source Type: research