Role of isoprostanes in human male infertility
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Source: Systems Biology in Reproductive Medicine - August 25, 2020 Category: Sexual Medicine Authors: Cinzia Signorini Elena Moretti Giulia Collodel Source Type: research

Role of isoprostanes in human male infertility.
This article discusses the role of isoprostanes as relevant factors both to sperm FA composition and sperm membrane integrity. Additionally, isoprostane's influence on sperm quality is reviewed. With reference to male reproductive dysfunction, increasing evidence indicates isoprostanes, detectable in biological fluids or sperm membrane, as the specific index of 1) exposure to chemical etiological agents, 2) oxidative damage, 3) reduced antioxidant response, and 4) sperm immaturity. ABBREVIATIONS: OS: oxidative stress; ROS: reactive oxygen species; PUFAs: polyunsaturated fatty acids; ARA: arachidonic acid, F2-IsoPs; F2...
Source: Systems Biology in Reproductive Medicine - August 24, 2020 Category: Reproduction Medicine Authors: Signorini C, Moretti E, Collodel G Tags: Syst Biol Reprod Med Source Type: research

The role of environmental optimization for storing bulls' sperm cells.
Abstract Artificial insemination has achieved a dynamic increase in genetic progress, and this is due to the improvement of sperm preservation technology. In recent years, a lot of attention has been paid to optimizing bull sperm storage environment and objectifying methods of sperm quality analysis. This review presents bull sperm preservation methods and ways to modify their storage environment. The main purpose of sperm preparation for artificial insemination is to obtain sperm with a high percentage of viable, motile sperm with normal morphology and low DNA fragmentation rates. Currently conducted experiments ...
Source: Systems Biology in Reproductive Medicine - August 17, 2020 Category: Reproduction Medicine Authors: Kowalczyk A, Kuczaj M, Czerniawska-Piątkowska E Tags: Syst Biol Reprod Med Source Type: research

The role of environmental optimization for storing bulls ’ sperm cells
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Source: Systems Biology in Reproductive Medicine - August 17, 2020 Category: Sexual Medicine Authors: Alicja Kowalczyk Marian Kuczaj Ewa Czerniawska-Pi ątkowska Source Type: research

Transforming growth factor beta 1 (TGF β1) polymorphisms and unexplained infertility: A genetic association study
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Source: Systems Biology in Reproductive Medicine - July 30, 2020 Category: Sexual Medicine Authors: Farnaz Marhemati Ramazan Rezaei Anahita Mohseni Meybodi Robabeh Taheripanah Shayan Mostafaei Davar Amani Source Type: research

Transforming growth factor beta 1 (TGF β1) polymorphisms and unexplained infertility: A genetic association study.
Transforming growth factor beta 1 (TGFβ1) polymorphisms and unexplained infertility: A genetic association study. Syst Biol Reprod Med. 2020 Jul 31;:1-14 Authors: Marhemati F, Rezaei R, Mohseni Meybodi A, Taheripanah R, Mostafaei S, Amani D Abstract The prevalence of infertility is increasing and worrisome. About 10 to 30% of infertility is classified as idiopathic or unexplained infertility (UI).TGF-β is multifunctional and immunoregulatry cytokine which regulates both implantation and adhesion of trophoblasts to the extracellular matrix during pregnancy. The aim of the current study was to...
Source: Systems Biology in Reproductive Medicine - July 30, 2020 Category: Reproduction Medicine Authors: Marhemati F, Rezaei R, Mohseni Meybodi A, Taheripanah R, Mostafaei S, Amani D Tags: Syst Biol Reprod Med Source Type: research

Effects of testicular sperm aspiration upon first cycle ICSI-ET for type 2 diabetic male patients.
Abstract Male diabetes mellitus (DM) can affect erectile function and sperm quality. In severe cases, DM can lead to retrograde or no ejaculation, so testicular sperm aspiration (TESA) is combined with intracytoplasmic sperm injection (ICSI) to treat subfertility and infertility for DM couples. However, the effect of TESA upon ICSI (TESA-ICSI) for DM patients remains unclear. This research investigated the effect of TESA-ICSI on first cycle ICSI-embryo transfer (ICSI-ET) for type 2 diabetic mellitus (T2DM) patients and the potential mechanisms. The subjects consisted of 1219 male patients with azoospermia or ...
Source: Systems Biology in Reproductive Medicine - July 26, 2020 Category: Reproduction Medicine Authors: Liu X, Gao M, Sun J, Sun Z, Song J, Xue X, Zhang Z, Shi J, Xing J Tags: Syst Biol Reprod Med Source Type: research

Effects of testicular sperm aspiration upon first cycle ICSI-ET for type 2 diabetic male patients
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Source: Systems Biology in Reproductive Medicine - July 26, 2020 Category: Sexual Medicine Authors: Xiang Liu Ming Gao Jianhua Sun Zheng Sun Juan Song Xia Xue Zhou Zhang Juanzi Shi Junping Xing Source Type: research

A method to improve embryo development potential when fertilization is delayed in mice.
Abstract The purpose of this study was to explore the application of glucose to improve embryo development potential when fertilization is delayed in mice. After recovery, mouse oocytes were cultured alone for 6 h before fertilization in three fertilization media: G-IVF PLUS, G-IVF PLUS with 5 mM and 10 mM glucose. G-IVF PLUS group was used as the control group. Then, in vitro fertilization (IVF) was performed and blastocysts were transferred at Day 4. To compare the effects of different glucose concentrations on embryo development and birth outcomes, conventional IVF and embryo transfer were carrie...
Source: Systems Biology in Reproductive Medicine - June 29, 2020 Category: Reproduction Medicine Authors: Chu D, Wang H, Fu L, Zhou W, Li Y Tags: Syst Biol Reprod Med Source Type: research

Monocyte chemotactic protein-1 plays a role in ovarian dysfunction related to high-fat diet-induced obesity.
Abstract Obesity, known to cause a systemic elevation in monocyte chemotactic protein-1 (MCP-1), adversely affects normal ovarian function. The aim of this study was to determine whether MCP-1 plays a role in ovarian dysfunction that is related to obesity induced by high-fat (HF) diet intake. Wild type (WT) C57BL/6J mice were fed either normal chow (NC) (Group 1, control group) or HF diet (Group 2). To assess whether MCP-1 is involved in HF-diet-induced ovarian dysfunction, MCP-1 knock-out mice were fed HF diet (Group 3). Body weight, body fat composition, number of oocytes collected following ovarian superovulati...
Source: Systems Biology in Reproductive Medicine - June 29, 2020 Category: Reproduction Medicine Authors: Asemota O, Thornton K, Merhi Z, Berger DS, Williams L, Seki Y, Du XQ, Jindal S, Charron MJ, Buyuk E Tags: Syst Biol Reprod Med Source Type: research

A method to improve embryo development potential when fertilization is delayed in mice
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Source: Systems Biology in Reproductive Medicine - June 29, 2020 Category: Sexual Medicine Authors: Dapeng Chu Haiping Wang Lei Fu Wenhui Zhou Yuan Li Source Type: research

Monocyte chemotactic protein-1 plays a role in ovarian dysfunction related to high-fat diet-induced obesity
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Source: Systems Biology in Reproductive Medicine - June 29, 2020 Category: Sexual Medicine Authors: Obehi Asemota Kimberley Thornton Zaher Merhi Dara S. Berger Lyda Williams Yoshinori Seki Xiu Quan Du Sangita Jindal Maureen J. Charron Erkan Buyuk Source Type: research

The limited knowledge of placental damage due to neglected infections: ongoing problems in Latin America
Volume 66, Issue 3, June 2020, Page 151-169 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 1, 2020 Category: Sexual Medicine Authors: Isabela Moreti Ribeiro Paula Cristina Souza Souto Alexandre U. Borbely Eloiza Lopes Lira Tanabe Angela Cadavid Angela M. Alvarez Julio Bueno Olga Agudelo Reggie Garc ía Robles Paola Ayala-Ram írez Flavia Sacerdoti Theodora Szasz Alicia E. Damiano Cristi Source Type: research

The limited knowledge of placental damage due to neglected infections: ongoing problems in Latin America.
ni FR Abstract The placenta works as a selective barrier, protecting the fetus from potential infections that may affect the maternal organism during pregnancy. In this review, we will discuss several challenging infections that are common within Latin American countries and that may affect the maternal-fetal interface and pose risks to fetal development. Specifically, we will focus on emerging infectious diseases including the arboviruses, malaria, leishmaniasis, and the bacterial foodborne disease caused by Shiga toxin-producing Escherichia coli. We will also highlight some topics of interest currently being stu...
Source: Systems Biology in Reproductive Medicine - May 31, 2020 Category: Reproduction Medicine Authors: Ribeiro IM, Souto PCS, Borbely AU, Tanabe ELL, Cadavid A, Alvarez AM, Bueno J, Agudelo O, Robles RG, Ayala-Ramírez P, Sacerdoti F, Szasz T, Damiano AE, Ibarra C, Escudero C, Lima VV, Giachini FR Tags: Syst Biol Reprod Med Source Type: research

Comparison between embryos transferred with self-spent and fresh medium on reproductive outcomes: a prospective randomized trial.
Abstract As the final and critical step in in vitro fertilization (IVF), embryo transfer has always received much attention and deserves continuous optimization. In the present study, to explore the role of autocrine factors in embryo self-spent culture media, we prospectively compared embryo transfer with self-spent culture medium and fresh medium on clinical pregnancy outcomes. A total of 318 fresh IVF/intracytoplasmic sperm injection (ICSI) cycles were randomly allocated into two subgroups based on their transfer media (using a self-spent culture medium or new pre-equilibrated culture media), and the clinical o...
Source: Systems Biology in Reproductive Medicine - May 30, 2020 Category: Reproduction Medicine Authors: Wu B, Yan B, Hu R, Tian S, Ni Y, Liang Y, Wang Y, Zhang Y Tags: Syst Biol Reprod Med Source Type: research

Comparison between embryos transferred with self-spent and fresh medium on reproductive outcomes: a prospective randomized trial
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Source: Systems Biology in Reproductive Medicine - May 30, 2020 Category: Sexual Medicine Authors: Bin Wu Bo Yan Rui Hu Shan Tian Yali Ni Yu Liang Yunshan Wang Yingchun Zhang Source Type: research

Toxicity and antimicrobial effect of silver nanoparticles in swine sperms
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Source: Systems Biology in Reproductive Medicine - May 26, 2020 Category: Sexual Medicine Authors: Francisco P érez-Duran Laura Susana Acosta-Torres Paloma Netzayeli Serrano-D íaz Irma Arcelia Toscano-Torres Ingrid Brenda Olivo-Zepeda Edwin Garc ía-Caxin Rosa Elvira Nu ñez-Anita Source Type: research

Toxicity and antimicrobial effect of silver nanoparticles in swine sperms.
, Nuñez-Anita RE Abstract Bacterial contamination in swine semen affects the quality and longevity of sperm and consequently fertility is reduced. Antibiotics have been used to prevent bacterial growth, but the frequency of bacterial resistance to various antibiotics are increasing. Silver nanoparticles (AgNPs) of 10-20 nm in size have shown a biocide effect in bacteria and fungi microorganisms without toxicity to certain mammalian cells. The goal of this study was to analyze both, antimicrobial activity against Staphylococcus aureus and toxicity in swine sperms after 10-20 nm AgNPs treatment. S. ...
Source: Systems Biology in Reproductive Medicine - May 25, 2020 Category: Reproduction Medicine Authors: Pérez-Duran F, Acosta-Torres LS, Serrano-Díaz PN, Toscano-Torres IA, Olivo-Zepeda IB, García-Caxin E, Nuñez-Anita RE Tags: Syst Biol Reprod Med Source Type: research

Co-expressed functional module-related genes in ovarian cancer stem cells represent novel prognostic biomarkers in ovarian cancer
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Source: Systems Biology in Reproductive Medicine - May 21, 2020 Category: Sexual Medicine Authors: Esra Gov Source Type: research

Re-determination of upper reference range of follicular stimulating hormone in infertile men
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Source: Systems Biology in Reproductive Medicine - May 21, 2020 Category: Sexual Medicine Authors: Muharrem Ozkaya Unal Oztekin Mehmet Caniklioglu Oguz Ekmekcioglu Source Type: research

Co-expressed functional module-related genes in ovarian cancer stem cells represent novel prognostic biomarkers in ovarian cancer.
Abstract Ovarian cancer is the leading cause of death from gynecologic malignancies. Cancer stem cells (CSC) seem to play a crucial role in tumor metastasis, recurrence, and chemoresistance. Therefore, CSCs offer significant potential for developing therapeutic targets and to understand tumor recurrence and chemoresistance mechanisms. In the present study, our aim was the identification of the gene group in ovarian CSCs (O-CSCs) and the potential of the resultant gene group in ovarian cancer prognosis. Two different microarray data sets were analyzed by comparing gene expression levels between O-CSCs and cancer sa...
Source: Systems Biology in Reproductive Medicine - May 21, 2020 Category: Reproduction Medicine Authors: Gov E Tags: Syst Biol Reprod Med Source Type: research

Re-determination of upper reference range of follicular stimulating hormone in infertile men.
In this study 1,893 infertile male patients were evaluated retrospectively. Physical examination, hormone analysis (total testosterone (TT), FSH, luteinizing hormone (LH), estradiol (E2), sex hormone binding globulin (SHBG) and prolactin (PRL)), semen analyzes were recorded and analyzed retrospectively. Logistic regression analysis, 95% confidence intervals and probability ratios were calculated to show the relationship between categorical hormone levels (quarters) and semen parameters. Hormone levels were categorized using the distribution quarters in the study population. FSH values of 62% of the cases with sperm concentration
Source: Systems Biology in Reproductive Medicine - May 21, 2020 Category: Reproduction Medicine Authors: Ozkaya M, Oztekin U, Caniklioglu M, Ekmekcioglu O Tags: Syst Biol Reprod Med Source Type: research

Effect of epigallocatechin-3-gallate (EGCG) on embryos inseminated with oxidative stress-induced DNA damage sperm.
Abstract Cryopreservation can induce damage in human spermatozoa through reactive oxygen species (ROS) generation. To reduce the potential risk of oxidative stress-induced sperm DNA damage, addition of different epigallocatechin-3-gallate (EGCG) concentrations were performed to determine the optimum concentration which was beneficial for IVF outcome for both fresh and frozen-thawed sperm. Next, the mouse sperm model exhibiting oxidative stress-induced DNA damage by exogenously treating with H2O2 but overcoming the low fertilization rate of frozen-thawed sperm was used to investigate the effect of EGCG on the embry...
Source: Systems Biology in Reproductive Medicine - May 18, 2020 Category: Reproduction Medicine Authors: Chen M, Liu W, Li Z, Xiao W Tags: Syst Biol Reprod Med Source Type: research

Effect of epigallocatechin-3-gallate (EGCG) on embryos inseminated with oxidative stress-induced DNA damage sperm
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Source: Systems Biology in Reproductive Medicine - May 18, 2020 Category: Sexual Medicine Authors: Man Chen Wanmin Liu Zhiling Li Wanfen Xiao Source Type: research

The effect of low and ultra-low oxygen tensions on mammalian embryo culture and development in experimental and clinical IVF.
This article reviews the literature data of the last four decades and discusses the current evidence on the use of low and ultra-low O2 in embryo culture, and examines the impact of multiple factors on IVF outcomes.Abbreviations: O2: oxygen tension; IVF: in vitro fertilization; IVC: in vitro culture; ET: embryo transfer; ROS: reactive oxygen species; ARTs: assisted reproductive technologies. PMID: 32379506 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - May 6, 2020 Category: Reproduction Medicine Authors: Belli M, Antonouli S, Palmerini MG, Bianchi S, Bernardi S, Khalili MA, Donfrancesco O, Nottola SA, Macchiarelli G Tags: Syst Biol Reprod Med Source Type: research

The effect of low and ultra-low oxygen tensions on mammalian embryo culture and development in experimental and clinical IVF
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Source: Systems Biology in Reproductive Medicine - May 6, 2020 Category: Sexual Medicine Authors: Manuel Belli Sevastiani Antonouli Maria Grazia Palmerini Serena Bianchi Sara Bernardi Mohammad Ali Khalili Orlando Donfrancesco Stefania Annarita Nottola Guido Macchiarelli Source Type: research

Flaws (and quality) in research today: can artificial intelligence intervene?
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Source: Systems Biology in Reproductive Medicine - April 7, 2020 Category: Sexual Medicine Authors: Charalampos Siristatidis Abraham Pouliakis Source Type: research

Optimizing the protocol for vitrification of individual spermatozoa by adjusting equilibration time.
Abstract Efficient cryopreservation of small numbers of human spermatozoa is essential in cases of severe male infertility, especially those requiring surgical sperm retrieval. Although vitrifying individual spermatozoa on sperm vitrification devices (SpermVD®) provided optimal cell retrieval upon warming, motility rates tended to be lower than with bulk-freezing. Post-warming motility is directly affected by cryoprotectant exposure; however, optimal cryoprotectant equilibration time is unknown. We evaluated several timeframes exposing individual spermatozoa to cryoprotectant before freezing and different cryo...
Source: Systems Biology in Reproductive Medicine - March 23, 2020 Category: Reproduction Medicine Authors: Belenky M, Itzhakov D, Freger V, Roseman O, Abehsera S, Miller N, Berkovitz A Tags: Syst Biol Reprod Med Source Type: research

Optimizing the protocol for vitrification of individual spermatozoa by adjusting equilibration time
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Source: Systems Biology in Reproductive Medicine - March 23, 2020 Category: Sexual Medicine Authors: Michael Belenky Diana Itzhakov Vita Freger Orna Roseman Sarah Abehsera Netanella Miller Arie Berkovitz Source Type: research

Microstructures of the spermatic cord with three-dimensional reconstruction of sections of the cord and application to varicocele.
Abstract The aim of the study was to investigate the micro-structures of the spermatic cord using histological examination with three-dimensional (3D) reconstruction of the serial tissue sections of the cord for clinical application in microscopic varicocelectomy. Human spermatic cord specimens obtained from 13 adult male cadavers were used to prepare serial transverse sections. The sections were stained to allow observation of the spermatic cord microstructures. The 3D reconstruction was performed with digitized serial sections by Mimics software. The microscopic varicocelectomy was performed based on the anatomi...
Source: Systems Biology in Reproductive Medicine - March 22, 2020 Category: Reproduction Medicine Authors: Yang Y, Wu X, Leng Q, Su W, Wang S, Xing R, Zhou X, Lv D, Li B, Mao X Tags: Syst Biol Reprod Med Source Type: research

Microstructures of the spermatic cord with three-dimensional reconstruction of sections of the cord and application to varicocele
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Source: Systems Biology in Reproductive Medicine - March 22, 2020 Category: Sexual Medicine Authors: Yu Yang Xiaoqiang Wu Qu Leng Wei Su Shuo Wang Rongwei Xing Xumin Zhou Daojun Lv Bingkun Li Xiangming Mao Source Type: research

Comparison between semen parameters in specimens collected early in the morning and in the evening.
Abstract The majority of facilities in Japan that offer artificial insemination as part of assisted reproduction programs currently perform semen collection in the early morning. The total motile sperm count of the semen used in intrauterine insemination is an important factor in achieving successful fertilization and subsequent childbirth. The present study was initiated to determine whether semen parameters varied with the time of day at which the semen sample was collected. The study subjects were 20 fertile males and 20 infertile males with abnormal seminograms who attended our Reproduction Center. Semen was c...
Source: Systems Biology in Reproductive Medicine - March 19, 2020 Category: Reproduction Medicine Authors: Shimomura Y, Shin T, Osaka A, Inoue Y, Iwahata T, Kobori Y, Ide H, Soh S, Okada H Tags: Syst Biol Reprod Med Source Type: research

Comparison between semen parameters in specimens collected early in the morning and in the evening
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Source: Systems Biology in Reproductive Medicine - March 19, 2020 Category: Sexual Medicine Authors: Yukihito Shimomura Takeshi Shin Akiyoshi Osaka Yasuyuki Inoue Toshiyuki Iwahata Yoshitomo Kobori Hisamitsu Ide Shigehiro Soh Hiroshi Okada Source Type: research

Versican expression level in cumulus cells is associated with human oocyte developmental competence.
In conclusion, the level of VCAN expression in cumulus cells is positively correlated with the early embryo morphology score, and with further development could perhaps be used to evaluate oocyte developmental competence to complement embryonic morphological assessment.Abbreviations: CCs: cumulus cells; GDF9: growth differentiation factor 9; BMP15: bone morphogenetic protein 15; PTGS2: prostaglandin synthase 2; HAS2: hyaluronic acid synthase 2; VCAN: versican; GREM1: gremlin 1; PFKP: phosphofructokinase, platelet; PKM2: pyruvate kinase isozyme type M2; LDHA: lactic dehydrogenase; GFPT: glucosaminefructo-6-phosphate transam...
Source: Systems Biology in Reproductive Medicine - March 5, 2020 Category: Reproduction Medicine Authors: Shen Q, Chen M, Zhao X, Liu Y, Ren X, Zhang L Tags: Syst Biol Reprod Med Source Type: research

Differentiation of neonate mouse spermatogonial stem cells on three-dimensional agar/polyvinyl alcohol nanofiber scaffold.
In this study, we aimed to examine the effects of agar/polyvinyl alcohol nanofiber (PVA) scaffold on the proliferation efficiency and differentiation potential of neonate mouse spermatogonial stem cells (SCCs). Testicular cells were isolated from testes of 40 mouse pups and were seeded in: 1) 2D cell culture plates in the absence (2D/-GF) or presence (2D/+GF) of growth factors and 2) onto agar/PVA scaffold in the absence (3D/-GF) or presence (3D/+GF) of growth factors. The cells were subsequently cultured for 4 weeks. First 2 weeks were dedicated to proliferative phase, whereas the next 2 weeks emphasized the differen...
Source: Systems Biology in Reproductive Medicine - March 5, 2020 Category: Reproduction Medicine Authors: Ziloochi Kashani M, Bagher Z, Asgari HR, Najafi M, Koruji M, Mehraein F Tags: Syst Biol Reprod Med Source Type: research

Differentiation of neonate mouse spermatogonial stem cells on three-dimensional agar/polyvinyl alcohol nanofiber scaffold
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Source: Systems Biology in Reproductive Medicine - March 5, 2020 Category: Sexual Medicine Authors: Marzieh Ziloochi Kashani Zohreh Bagher Hamid Reza Asgari Mohammad Najafi Morteza Koruji Fereshteh Mehraein Source Type: research

Versican expression level in cumulus cells is associated with human oocyte developmental competence
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Source: Systems Biology in Reproductive Medicine - March 5, 2020 Category: Sexual Medicine Authors: Qiuzi Shen Mei Chen Xue Zhao Yu Liu Xinling Ren Ling Zhang Source Type: research

2019 Systems Biology in Reproductive Medicine Selected Highlights
Volume 66, Issue 1, February 2020, Page 76-78 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - March 4, 2020 Category: Sexual Medicine Source Type: research

Moving forward in 2020 integrating systems biology in reproductive medicine
Volume 66, Issue 1, February 2020, Page 1-2 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - March 4, 2020 Category: Sexual Medicine Authors: Stephen A. Krawetz Source Type: research

The impact of hyaluronan-enriched culture medium and intrauterine infusion of human chorionic gonadotropin on clinical outcomes in blastocyst transfer cycles.
Abstract Over the last few decades, advances in ovarian hormonal stimulation, embryology laboratory technologies and embryo genetic testing, have significantly enhanced clinical outcomes in human assisted reproduction technologies (ART). However, embryo implantation remains a major bottleneck in achieving better pregnancy and live birth rates. Thus, there is growing interest in establishing new approaches to enhance implantation efficiency after embryo transfer. With advanced molecular techniques, many promising biomarkers associated with embryonic and endometrial changes occurring prior to and during embryo impla...
Source: Systems Biology in Reproductive Medicine - March 3, 2020 Category: Reproduction Medicine Authors: Balakier H, Kuznyetsova I, Librach CL Tags: Syst Biol Reprod Med Source Type: research

The impact of hyaluronan-enriched culture medium and intrauterine infusion of human chorionic gonadotropin on clinical outcomes in blastocyst transfer cycles.
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Source: Systems Biology in Reproductive Medicine - March 3, 2020 Category: Sexual Medicine Authors: Hanna Balakier Iryna Kuznyetsova Clifford L. Librach Source Type: research

Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy
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Source: Systems Biology in Reproductive Medicine - February 17, 2020 Category: Sexual Medicine Authors: Marcello Rubessa Mikhail E. Kandel Sierra Schreiber Sasha Meyers Douglas H. Beck Gabriel Popescu Matthew B. Wheeler Source Type: research

Early and late paternal effects of reactive oxygen species in semen on embryo development after intracytoplasmic sperm injection
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Source: Systems Biology in Reproductive Medicine - February 16, 2020 Category: Sexual Medicine Authors: Shinnosuke Kuroda Teppei Takeshima Kazumi Takeshima Kimitsugu Usui Kengo Yasuda Hiroyuki Sanjo Takashi Kawahara Hiroji Uemura Mariko Murase Yasushi Yumura Source Type: research

Early and late paternal effects of reactive oxygen species in semen on embryo development after intracytoplasmic sperm injection.
Abstract Although reactive oxygen species in semen are associated with unfavorable results with respect to assisted reproductive technology, their effects based on the detailed stages of embryo development are unclear. We investigated the relationship between reactive oxygen species in semen and the oocyte fertilization rate, cleavage rate, and blastulation rate of intracytoplasmic sperm injections. This retrospective study enrolled 77 couples who underwent intracytoplasmic sperm injection and analyzed 887 eggs from 141 cycles of intracytoplasmic sperm injection. The reactive oxygen species level in semen was comp...
Source: Systems Biology in Reproductive Medicine - February 15, 2020 Category: Reproduction Medicine Authors: Kuroda S, Takeshima T, Takeshima K, Usui K, Yasuda K, Sanjo H, Kawahara T, Uemura H, Murase M, Yumura Y Tags: Syst Biol Reprod Med Source Type: research

Follicular fat-soluble vitamins as markers of oocyte competency.
Abstract The main aim of this prospective study was to investigate the effect of the concentration of fat-soluble vitamins A, D, E and K in individual follicles on oocyte quality and developmental competence. The analysis was performed on 313 follicular fluid (FF) samples from 50 patients undergoing ovarian stimulation with intracytoplasmic sperm injection. We demonstrated that the mean concentration of individual vitamins in FF correlated with their level in serum (p
Source: Systems Biology in Reproductive Medicine - February 13, 2020 Category: Reproduction Medicine Authors: Skowrońska P, Kunicki M, Pastuszek E, Konieczna L, Bączek T, Łukaszuk K Tags: Syst Biol Reprod Med Source Type: research

Follicular fat-soluble vitamins as markers of oocyte competency
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Source: Systems Biology in Reproductive Medicine - February 13, 2020 Category: Sexual Medicine Authors: Patrycja Skowro ńska Micha ł Kunicki Ewa Pastuszek Lucyna Konieczna Tomasz B ączek Krzysztof Łukaszuk Source Type: research

Comprehensive profiling of Small RNAs in human embryo-conditioned culture media by improved sequencing and quantitative PCR methods.
We report the first comprehensive sequencing of sncRNAs in ECCM with a sequencing sensitivity of 3 single embryo drops, capturing ~150 miRNAs and an abundance of tRNA-derived small RNAs (tsRNAs). We then profiled 15 sncRNAs by qPCR and determined that the assay maintains sensitivity in single ECCM drops. Finally, we found significant differences in these sncRNA expression between control and ECCM drops. Improving embryo selection is crucial for reducing time to pregnancy. Here we describe a sensitive technique for biomarker discovery by sequencing and qPCR validation in ECCM, demonstrating that the majority of sncRNAs are ...
Source: Systems Biology in Reproductive Medicine - February 12, 2020 Category: Reproduction Medicine Authors: Russell SJ, Menezes K, Balakier H, Librach C Tags: Syst Biol Reprod Med Source Type: research

Comprehensive profiling of Small RNAs in human embryo-conditioned culture media by improved sequencing and quantitative PCR methods
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Source: Systems Biology in Reproductive Medicine - February 12, 2020 Category: Sexual Medicine Authors: Stewart J. Russell Karen Menezes Hanna Balakier Clifford Librach Source Type: research

Transcriptional alterations of genes related to fertility decline in male rats induced by chronic sleep restriction.
In this study, a rat model of chronic sleep restriction (CSR) was established using the modified multiplatform method. The effects of CSR on the fertility of male rats were evaluated first based on sexual behavior. Serum hormones, including testosterone (T), prolactin (PRL), luteinizing hormone (LH) and follicle-stimulating hormone (FSH), and sperm parameters (concentration, viability, motility, deformation rate) were measured, and testicular histology was analysed by hematoxylin and eosin staining. The transcriptional differences between CSR rats and control rats were detected by RNA sequencing (RNA-Seq), and DNA methylat...
Source: Systems Biology in Reproductive Medicine - February 9, 2020 Category: Reproduction Medicine Authors: Chen W, Guo X, Jin Z, Li R, Shen L, Li W, Cai W, Zhang G Tags: Syst Biol Reprod Med Source Type: research

Transcriptional alterations of genes related to fertility decline in male rats induced by chronic sleep restriction
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Source: Systems Biology in Reproductive Medicine - February 9, 2020 Category: Sexual Medicine Authors: Wenyang Chen Xingdao Guo Zhiping Jin Runan Li Lixia Shen Wei Li Wangting Cai Guirong Zhang Source Type: research