Effects of high progesterone on outcomes of in vitro fertilization-embryo transfer in patients with different ovarian responses.
Abstract The data of 3,841 cycles undergoing in vitro fertilization-embryo transfer (IVF-ET) in our reproductive Center between January 2003 and December 2013 were retrospectively analyzed. According to the number of oocytes retrieved, this study was divided into the high ovarian response group (oocyte retrieval ≥20, 842 cycles), the moderate ovarian response group (5
Source: Systems Biology in Reproductive Medicine - April 27, 2015 Category: Reproduction Medicine Authors: Li PF, Zhu H, Tan L, Zhao DM, Ma LY, Xiang YG, Zhang D, Dou Q, Lu N Tags: Syst Biol Reprod Med Source Type: research

In Silico analysis of perturbed steroidogenesis and gonad growth in fathead minnows (P. promelas) exposed to 17α-ethynylestradiol.
In this study, an in silico stoichiometric model of piscine steroidogenesis was augmented with a 'biomass' reaction associating the underlying stoichiometry of steroidogenesis with a reaction representative of gonad growth. The ability of the in silico model to predict perturbed steroidogenesis and subsequent effects on gonad growth was tested by exposing reproductively active male and female fathead minnows (Pimephales promelas) to 88 ng/L of the synthetic estrogen, 17α-ethynylestradiol (EE2). The in silico model was parameterized (or constrained) with experimentally quantified concentrations of selected ster...
Source: Systems Biology in Reproductive Medicine - April 24, 2015 Category: Reproduction Medicine Authors: Hala D, Petersen LH, Martinović D, Huggett DB Tags: Syst Biol Reprod Med Source Type: research

Successful outcomes achieved in assisted reproduction cycles using sperm with high levels of high DNA stainability.
Abstract The sperm chromatin structure assay (SCSA) has been proposed as a useful addition to the battery of tests routinely used to explore semen quality and hence to give an indication of the likelihood of a successful pregnancy. As usually performed at present, the assay yields two main sperm variables, the DNA fragmentation index (DFI) and the high DNA stainability (HDS). In the present study 275 patients undergoing 215 in vitro fertilization (IVF) and 215 intracytoplasmic sperm injection (ICSI) cycles were studied with the purpose of defining the clinical significance of HDS in IVF and ICSI cycles. Using the ...
Source: Systems Biology in Reproductive Medicine - April 21, 2015 Category: Reproduction Medicine Authors: Speyer BE, Pizzey AR, Abramov B, Saab W, Doshi A, Sarna U, Harper JC, Serhal P Tags: Syst Biol Reprod Med Source Type: research

Intrinsic and extrinsic apoptotic pathways are involved in rat testis by cold water immersion-induced acute and chronic stress.
Del Socorro Retana-Márquez M Abstract Testicular apoptosis is activated by stress, but it is not clear which signaling pathway is activated in response to stress. The aim of this study was to investigate whether intrinsic, extrinsic, or both apoptotic signaling pathways are activated by acute and chronic stress. Adult male rats were subjected to cold water immersion-induced stress for 1, 20, 40, and 50 consecutive days. The seminiferous tubules:apoptotic cell ratio was assayed on acute (1 day) and chronic (20, 40, 50 days) stress. Apoptotic markers, including cleaved-caspase 3 and 8, the pro-apoptotic Bax a...
Source: Systems Biology in Reproductive Medicine - April 13, 2015 Category: Reproduction Medicine Authors: Juárez-Rojas AL, García-Lorenzana M, Aragón-Martínez A, Gómez-Quiroz LE, Del Socorro Retana-Márquez M Tags: Syst Biol Reprod Med Source Type: research

A single nucleotide polymorphism in the MTOR gene is associated with recurrent spontaneous abortion in the Chinese female population.
Abstract Recurrent spontaneous abortion (RSA) is a multi-factor disease. The mammalian target of the the rapamycin (MTOR) gene has been reported to be involved in mouse embryo development and regulates the proliferation of embryonic stem cells. Our study explored the relationship between the single nucleotide polymorphism (SNP) rs17027478 in the promoter region of MTOR gene and the development of RSA. A total of 306 patients with RSA and 127 healthy females as the controls were recruited in the case-control study. The predesigned TaqMan SNP Genotyping Assay was adopted to analyze the association between rs17027478...
Source: Systems Biology in Reproductive Medicine - April 7, 2015 Category: Reproduction Medicine Authors: Xiang H, Liu S, Zong C, Li Z, Liu Y, Ma X, Cao Y Tags: Syst Biol Reprod Med Source Type: research

Sperm mRNAs and microRNAs as candidate markers for the impact of toxicants on human spermatogenesis: an application to tobacco smoking.
Abstract Spermatozoa contain a complex population of RNAs including messenger RNAs (mRNAs) and small RNAs such as microRNAs (miRNA). It has been reported that these RNAs can be used to understand the mechanisms by which toxicological exposure affects spermatogenesis. The aim of our study was to compare mRNA and miRNA profiles in spermatozoa from eight smokers and eight non-smokers, and search for potential relationships between mRNA and miRNA variation. All men were selected based on their answers to a standard toxic exposure questionnaire, and sperm parameters. Using mRNA and miRNA microarrays, we showed that mRN...
Source: Systems Biology in Reproductive Medicine - March 30, 2015 Category: Reproduction Medicine Authors: Metzler-Guillemain C, Victorero G, Lepoivre C, Bergon A, Yammine M, Perrin J, Sari-Minodier I, Boulanger N, Rihet P, Nguyen C Tags: Syst Biol Reprod Med Source Type: research

Induction of spermatogenesis by rhFSH for azoospermia due to spermatogenic dysfunction with maturation arrest: five case series.
Abstract When sperm cannot be retrieved from the testes of patients with azoospermia due to spermatogenic dysfunction (ASD), there is no rational way for the patient to become a biological father. We investigated the possibility of inducing spermatogenesis in such patients by hormonal therapy with recombinant human follicle-stimulating hormone (rhFSH) alone. Twenty-six ASD patients who could not obtain spermatozoa by microdissection testicular sperm extraction (micro-TESE) were confirmed to have arrested spermatogenesis at the late stage of maturation arrest. They were subsequently treated with 75-150 IU two times...
Source: Systems Biology in Reproductive Medicine - March 25, 2015 Category: Reproduction Medicine Authors: Kobori Y, Suzuki K, Iwahata T, Shin T, Sato R, Nishio K, Yagi H, Arai G, Soh S, Okada H Tags: Syst Biol Reprod Med Source Type: research

No association of TP53 codon 72 SNP with male infertility: a study in a Chinese population and a meta-analysis.
Abstract Abstract Genetic polymorphisms may affect human male fertility. Even though TP53 plays a role in spermatogenesis we know little about the association of the functional polymorphism at codon 72 of TP53 with respect to susceptibility to male infertility. We conducted a case-control study to investigate this association in a Chinese population and performed a meta-analysis in different populations to clarify this association. The single nucleotide polymorphism (SNP) of TP53 codon 72 (rs1042522 G>C) was genotyped by PCR-RFLP in 83 Chinese male infertility patients and 401 healthy controls. Meta-analysis wa...
Source: Systems Biology in Reproductive Medicine - March 6, 2015 Category: Reproduction Medicine Authors: Chan Y, Jiang H, Ma L, Chen J, Li D, Meng Y, Luo Y, Tang W Tags: Syst Biol Reprod Med Source Type: research

DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele.
This study aimed to assess and compare global DNA methylation (GDM) between fertile men and infertile men with varicocele. In addition, we evaluated the correlations between DNA methylation with reactive oxygen species (ROS), protamine deficiency, and DNA integrity. Semen samples were collected from 44 men with grades II and III varicocele, and 15 fertile men for assessment of semen parameters, DNA methylation, DNA fragmentation, oxidative stress, and protamine deficiency. Samples were evaluated by the World Health Organization (WHO) criteria, immunostaining, the TUNEL assay, 2', 7'-dichlorodihydrofluorescein diacetate (DC...
Source: Systems Biology in Reproductive Medicine - March 6, 2015 Category: Reproduction Medicine Authors: Bahreinian M, Tavalaee M, Abbasi H, Kiani-Esfahani A, Shiravi AH, Nasr-Esfahani MH Tags: Syst Biol Reprod Med Source Type: research

Effects of dehydroepiandrosterone (DHEA) on follicular dynamics in a diminished ovarian reserve in vivo model.
In conclusion, our findings suggest that DHEA administration in DOR rats due to VCD results in a larger follicular pool. Decreased atresia may be one of the possible effects of DHEA in DOR cases. Whatever the mechanism, DHEA treatment potentially may be useful clinically as a means to increase the number of gonadotropin-responsive follicles for ovarian stimulation. PMID: 25671506 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - February 11, 2015 Category: Reproduction Medicine Authors: Hassa H, Aydin Y, Ozatik O, Erol K Tags: Syst Biol Reprod Med Source Type: research

Can serum estradiol levels on the fourth day of IVF/ICSI cycle predict outcome in poor responder women?
Abstract Abstract We aimed to investigate the value of fourth day serum estradiol levels in predicting cycle outcome in poor responders. The medical records of 426 patients with low oocyte yield following controlled ovarian hyperstimulation (COH) treatment for a procedure in our institution's center for ART between 2008 and 2013 were evaluated. Eighty-eight patients exhibiting poor ovarian response (POR) were included in the study. The clinical outcomes of IVF/ICSI were compared based on the basal hormone profile, clinical, and laboratory parameters. Cycle day 4 E2 levels below 110 pg/ml had an odds ratio o...
Source: Systems Biology in Reproductive Medicine - February 11, 2015 Category: Reproduction Medicine Authors: Kahyaoglu S, Yumusak OH, Ozgu-Erdinc AS, Yilmaz S, Kahyaoglu I, Engin-Ustun Y, Yilmaz N Tags: Syst Biol Reprod Med Source Type: research

Oxidative status in testis and epididymal sperm parameters after acute and chronic stress by cold-water immersion in the adult rat.
The objective of this study was to characterize the role of oxidative stress induced by cold-water immersion stress in the testis of adult male rats and its relation with alterations in cauda epididymal sperm. Adult male rats were exposed to acute stress or chronic stress by cold-water immersion. Rats were sacrificed at 0, 6, 12, and 24 hours immediately following acute stress exposure, and after 20, 40, and 50 days of chronic stress. ROS production increased only at 6 hours post-stress, while the activity and expression of antioxidant enzymes, lipid peroxidation (LPO), and sperm parameters were not modified in the testis....
Source: Systems Biology in Reproductive Medicine - February 2, 2015 Category: Reproduction Medicine Authors: García-Díaz EC, Gómez-Quiroz LE, Arenas-Ríos E, Aragón-Martínez A, Ibarra-Arias JA, Retana-Márquez MD Tags: Syst Biol Reprod Med Source Type: research

A 24-chromosome FISH technique in preimplantation genetic diagnosis: validation of the method.
This study evaluates a novel 24-chromosome FISH technique protocol (FISH-24). A total of 337 embryos were analyzed using the traditional 9-chromosome FISH technique (FISH-9) while 251 embryos were evaluated using the new FISH-24 technique. Embryos deemed nontransferable on Day 3 were cultured in vitro to Day 5 of development, then fixed and reanalyzed according to the technique allocated to each treatment cycle (107 embryos analyzed by FISH-9 and 111 by FISH-24). The global error rate (discrepancy between Day 3 and Day 5 results for a single embryo) was 2.8% after FISH-9 and 3.6% after FISH-24, with a p value of 0.95. Thus...
Source: Systems Biology in Reproductive Medicine - January 13, 2015 Category: Reproduction Medicine Authors: Fernández SF, Toro E, Colomar A, López-Teijón M, Velilla E Tags: Syst Biol Reprod Med Source Type: research

[In Process Citation].
Abstract Re: Heidar Tavilani, Amir Fattahi, Maryam Esfahani, Iraj Khodadadi, Jamshid Karimi, Elham Bahrayni, et al. (2014) Genotype and phenotype frequencies of paraoxonase 1 in fertile and infertile men. Syst Biol Reprod Med 60(6):361-366. PMID: 25544566 [PubMed - in process] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - January 1, 2015 Category: Reproduction Medicine Authors: Agilli M, Aydin FN, Gulcan Kurt Y, Cayci T Tags: Syst Biol Reprod Med Source Type: research

Seminal quality in the first fraction of ejaculate.
e;nchez-Martín P Abstract Abstract Semen samples from 40 patients were collected in consecutive fractions. The variability in semen quality of each fraction was then determined. The first ejaculated fraction (FEF) primarily contained prostatic secretions, while the second ejaculate fraction (SEF) held the majority of the spermatozoa suspended in the secretions from the seminal vesicle. Differences in sperm quality were observed when the FEF was compared to the SEF and the total ejaculate fraction (TEF). These included the seminal parameters (volume, sperm concentration, motility) and sperm DNA fragmentation...
Source: Systems Biology in Reproductive Medicine - December 30, 2014 Category: Reproduction Medicine Authors: Hebles M, Dorado M, Gallardo M, González-Martínez M, Sánchez-Martín P Tags: Syst Biol Reprod Med Source Type: research

Alleviative effect of quercetin on rat testis against arsenic: a histological and biochemical study.
Abstract Abstract The preventive effect of quercetin on arsenic stimulated reproductive ailments in male Sprague Dawely (SD) rats was investigated. Twenty rats were divided into four groups. The first group served as a control and was provided tap water. The second group of rats was treated with sodium arsenite at the dose of 50 ppm in drinking water. The third group served as a positive control and received an oral dose of quercetin (50 mg/kg). In the fourth group, quercetin (50 mg/kg) was co-administered orally with arsenic (50 ppm in drinking water). All the treatments were carried o...
Source: Systems Biology in Reproductive Medicine - December 24, 2014 Category: Reproduction Medicine Authors: Jahan S, Iftikhar N, Ullah H, Rukh G, Hussain I Tags: Syst Biol Reprod Med Source Type: research

Hormonal therapy (hCG and rhFSH) for infertile men with adult-onset idiopathic hypogonadotropic hypogonadism.
This study was conducted to examine the efficacy and safety of a combination of human chorionic gonadotropin (hCG) and recombinant human follicle-stimulating hormone (rhFSH) for inducing spermatogenesis in men with adult-onset IMHH. Seven men (34-45 years of age) with azoospermia and/or sexual dysfunction, with a low serum testosterone concentration, and apulsatile secretion of luteinizing hormone, were referred to our hospital for infertility. All had normal secondary sexual characteristics. Thorough endocrinologic examination and magnetic resonance imaging revealed no identifiable cause of hypogonadotropic hypogonadism. ...
Source: Systems Biology in Reproductive Medicine - December 18, 2014 Category: Reproduction Medicine Authors: Kobori Y, Suzuki K, Iwahata T, Shin T, Sato R, Nishio K, Yagi H, Arai G, Soh S, Okada H Tags: Syst Biol Reprod Med Source Type: research

The balancing act - PPAR-γ's roles at the maternal-fetal interface.
The balancing act - PPAR-γ's roles at the maternal-fetal interface. Syst Biol Reprod Med. 2014 Dec 5;:1-7 Authors: Kadam L, Kohan-Ghadr HR, Drewlo S Abstract Abstract Peroxisome proliferator-activated receptor-gamma (PPAR-γ) belongs to the nuclear hormone receptor superfamily. Apart from being involved in lipid metabolism, like its other subtypes PPAR α and β, it is implicated to be crucial for successful placentation. While its role in extravillous trophoblast (EVT) differentiation has been studied, the involvement in villous trophoblast (VT) differentiation, fatty-acid metabol...
Source: Systems Biology in Reproductive Medicine - December 5, 2014 Category: Reproduction Medicine Authors: Kadam L, Kohan-Ghadr HR, Drewlo S Tags: Syst Biol Reprod Med Source Type: research

Embryo developmental potential of microsurgically corrected human three-pronuclear zygotes.
In this study, there were three groups, the 3 → 2PN group (338 zygotes), the non-corrected 3PN group (381 zygotes), and the normal 2PN group (359 zygotes). The first cleavage mode (2-cell cleavage or 3-cell cleavage), 6-8 cell embryogenesis rate, high-quality embryogenesis rate and Day 5/Day 6 blastulation rate were compared between the three groups. The success rate of enucleation was 92.9%. The 2-cell cleavage rate was significantly higher in the 3 → 2PN group (74.3%) than in the 3PN group (36.4%) (P  0.05). The 6-8 cell embryogenesis rate was significantly higher in th...
Source: Systems Biology in Reproductive Medicine - November 20, 2014 Category: Reproduction Medicine Authors: Jin HX, Dai SJ, Song WY, Yao GD, Shi SL, Sun YP Tags: Syst Biol Reprod Med Source Type: research

The effect of environmental exposure to pyrethroids and DNA damage in human sperm.
Abstract Abstract The present study was designed to investigate whether environmental exposure to pyrethroids was associated with sperm DNA damage. Between January 2008 and April 2011 286 men under 45 years of age with a normal sperm concentration of 15-300 10(6)/ml [WHO 2010] were recruited from an infertility clinic in Lodz, Poland. Participants were interviewed and provided urine, saliva, and semen samples. The pyrethroids metabolites: 3-phenoxybenzoic acid (3PBA), cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (CDCCA), trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic a...
Source: Systems Biology in Reproductive Medicine - November 7, 2014 Category: Reproduction Medicine Authors: Jurewicz J, Radwan M, Wielgomas B, Sobala W, Piskunowicz M, Radwan P, Bochenek M, Hanke W Tags: Syst Biol Reprod Med Source Type: research

Small supernumerary marker chromosomes and the nuclear architecture of sperm - a study in a fertile and an infertile brother.
Abstract Abstract Small supernumerary marker chromosomes (sSMC) are found about four times more frequently in subfertile compared to the general population. The reason for this finding is still unclear. However, a connection of interphase architecture and genome function is suggested. And as we found in a previous study the presence of sSMC influences the nuclear architecture of peripheral blood cells and fibroblasts, we hypothesized that sSMC could have similar effects in sperm cells possibly leading to infertility. Here we applied for the first time 3-dimensional interphase fluorescence in situ hybridization (3D...
Source: Systems Biology in Reproductive Medicine - November 6, 2014 Category: Reproduction Medicine Authors: Karamysheva T, Kosyakova N, Guediche N, Liehr T Tags: Syst Biol Reprod Med Source Type: research

Genetic association study of RNF8 and BRDT variants with non-obstructive azoospermia in the Chinese Han population.
Abstract Abstract Increasing evidence indicates that polymorphisms in genes relevant to spermatogenesis might modulate the efficiency of reproduction in men. Ring finger protein 8 (RNF8) and bromodomain testis-specific (BRDT) are two candidate genes associated with spermatogenesis. Here, we considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia (NOA). We analyzed 361 men with NOA and 368 fertile controls by using Sequenom iplex technology. Our data did not reveal any variants associated with NOA susceptibility. Howe...
Source: Systems Biology in Reproductive Medicine - November 6, 2014 Category: Reproduction Medicine Authors: Zhang Y, Song B, Du WD, He XJ, Ruan J, Zhou FS, Zuo XB, Ye L, Xie XS, Cao YX Tags: Syst Biol Reprod Med Source Type: research

Effect of sperm storage and selection techniques on sperm parameters.
This article will discuss these sperm preservation and selection techniques. PMID: 25354153 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - October 29, 2014 Category: Reproduction Medicine Authors: Sharma R, Kattoor AJ, Ghulmiyyah J, Agarwal A Tags: Syst Biol Reprod Med Source Type: research

The relationship between serum anti-Müllerian hormone levels and the follicular arrest for women with polycystic ovary syndrome.
The relationship between serum anti-Müllerian hormone levels and the follicular arrest for women with polycystic ovary syndrome. Syst Biol Reprod Med. 2014 Oct 20;:1-7 Authors: Li J, Li R, Yu H, Zhao S, Yu Y, Qiao J Abstract Abstract The aim of this study was to ascertain whether higher levels of serum anti-Müllerian hormone (AMH) are associated with the ovarian follicular arrest in women with polycystic ovary syndrome (PCOS). This prospective study compared AMH levels between serum and dominant follicular fluid (FF) in ovulatory polycystic ovary (PCO) women and anovulatory (menstrual cycle ...
Source: Systems Biology in Reproductive Medicine - October 20, 2014 Category: Reproduction Medicine Authors: Li J, Li R, Yu H, Zhao S, Yu Y, Qiao J Tags: Syst Biol Reprod Med Source Type: research

Influence of BHT inclusion on post-thaw attributes of human semen.
Abstract Abstract The aim of this study was to determine the effect of butylated hydroxytoluene (BHT) supplemented cryopreservation medium on sperm parameters during the freeze-thaw process. In addition, sperm lipid peroxidation, DNA damage, and the amount of reactive oxygen species (ROS) were determined. Semen samples were obtained from 75 donors. Fifteen semen samples were used for optimizing BHT concentration and incubation time and 60 samples were used for the final experiments. After the determination of basic parameters, groups of three sample with similar parameters were pooled and processed by Pure Sperm g...
Source: Systems Biology in Reproductive Medicine - October 6, 2014 Category: Reproduction Medicine Authors: Ghorbani M, Amiri I, Khodadadi I, Fattahi A, Atabakhsh M, Tavilani H Tags: Syst Biol Reprod Med Source Type: research

Genotype and phenotype frequencies of paraoxonase 1 in fertile and infertile men.
This study was aimed to investigate frequencies of phenotypes and Q192R genotypes of PON1 in fertile and infertile males. Q192R variants of PON1 were determined in 150 fertile and 150 infertile men using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Plasma arylesterase and paraoxonase activities were detected by spectrophotometry and malondialdehyde (MDA) level was measured using thiobarbituric acid. Our results showed no significant difference in the distribution of PON1 genotypes and alleles between fertile and infertile groups. However morphology and motility of sperm were ...
Source: Systems Biology in Reproductive Medicine - September 29, 2014 Category: Reproduction Medicine Authors: Tavilani H, Fattahi A, Esfahani M, Khodadadi I, Karimi J, Bahrayni E, Vatannejad A, Vaisi-Raygani A, Ghorbani M, Latifi Z Tags: Syst Biol Reprod Med Source Type: research

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes.
Abstract Abstract The aim of this study was to evaluate the influence of in vitro fertilization (IVF) versus intracytoplasmic sperm injection (ICSI), fertilization mode embryonic morphology at day 3, and female age on blastocyst development, on the clinical outcomes of pregnancy after blastocyst transfer. A total of 471 cycles were retrospectively investigated. The rates of blastocyst formation and of good blastocyst morphology were higher in IVF than in ICSI cycles but there were no significant differences in the clinical pregnancies or in the miscarriage rates. The rates of formation of blastocyst and of blastoc...
Source: Systems Biology in Reproductive Medicine - September 29, 2014 Category: Reproduction Medicine Authors: Yin H, Jiang H, He R, Wang C, Zhu J, Luan K Tags: Syst Biol Reprod Med Source Type: research

PGD for a carrier of an intrachromosomal insertion using aCGH.
We present a case of preimplantation genetic diagnosis (PGD) using array comparative genomic hybridization (aCGH) following trophectoderm biopsy of embryos created after in vitro fertilization for a carrier of an intrachromosomal insertion on chromosome 1 [46,XX, ins(1)(q44q23q32.1)]. The PGD analysis of 6 blastocysts demonstrated 67% unbalanced embryos. No pregnancy was achieved after the transfer of 2 euploid embryos. To the best of our knowledge, this is the first reported case of PGD using aCGH following trophectoderm biopsy for a carrier of an intrachromosomal insertion. PMID: 25247722 [PubMed - as supplied by pu...
Source: Systems Biology in Reproductive Medicine - September 23, 2014 Category: Reproduction Medicine Authors: Jones CA, Kolomietz E, Maire G, Vlasschaert M, Joseph-George AM, Myles-Reid D, Chong K, Chitayat D, Arthur R Tags: Syst Biol Reprod Med Source Type: research

Inflammatory bowel disease in subfertile men and the effect of mesalazine on fertility.
This study aimed to examine the effect of mesalazine on fertility. In this retrospective analysis of 1,225 male subfertile patients, we reviewed the cases of inflammatory bowel disease (IBD) in the medical records and evaluated the prevalence of the disease in our reproduction center. Specifically, we examined IBD patients who ceased mesalazine during male infertility treatment, and compared the seminogram of these patients before and after discontinuation of mesalazine. We also analyzed pregnancy outcome after discontinuation. The prevalence rate of Crohn's disease and ulcerative colitis in our male infertile patients was...
Source: Systems Biology in Reproductive Medicine - August 21, 2014 Category: Reproduction Medicine Authors: Shin T, Kobori Y, Suzuki K, Iwahata T, Yagi H, Soh S, Arai G, Okada H Tags: Syst Biol Reprod Med Source Type: research

Chronic alcohol administration causes expression of calprotectin and RAGE altering the distribution of zinc ions in mouse testis.
In this study we investigated whether chronic alcohol administration causes expression of CP and RAGE in mouse testis. The distribution of free and loosely bound Zn(2+) (FLB-Zn(2+)) in the testicular tissues was also evaluated. Alcohol-induced testicular damage was documented by measuring testosterone blood levels and by light and electron microscope studies. Twenty mice were treated daily for three weeks with 3.0 g/kg of a 25% solution of alcohol. Ten mice were treated in the same period of time with a solution of maltose dextrins, isocaloric to alcohol. Twenty untreated mice were used as controls. Alcohol treated ...
Source: Systems Biology in Reproductive Medicine - August 20, 2014 Category: Reproduction Medicine Authors: Giannessi F, Scavuzzo MC, Giambelluca MA, Fornai F, Morelli G, Ruffoli R Tags: Syst Biol Reprod Med Source Type: research

The effect of melatonin on procarbazine induced testicular toxicity on rats.
, Erdem O Abstract Abstract Procarbazine (P) is an effective chemotherapeutic drug especially used in lymphoma treatment; however testicular toxicity is a limiting factor. Various ways of treatment were tried to preserve testicular function including hormonal treatment, antioxidant treatment, and sperm cryopreservation but resulted with low rates of satisfaction. Procarbazine is a well known agent causing sterility even in the first doses of chemotherapy. Antioxidants such as N acetylcysteine and ascorbate have been used for protective purposes and were very successful. Melatonin (M) is another powerful antioxidan...
Source: Systems Biology in Reproductive Medicine - August 20, 2014 Category: Reproduction Medicine Authors: Alp BF, Kesik V, Malkoç E, Yiğit N, Saldır M, Babacan O, Akgül EO, Poyraz Y, Korkmazer N, Gulgun M, Erdem O Tags: Syst Biol Reprod Med Source Type: research

A randomized trial comparing time intervals from HCG trigger to intrauterine insemination for cycles utilizing GnRH antagonists.
Abstract Abstract Intrauterine insemination (IUI) during ovarian stimulation cycles is typically performed 36 hours after human chorionic gonadotropin (hCG) injection. We hypothesized that adjusting the time interval to IUI to better coincide with ovulation may increase pregnancy rates. Patients undergoing induction of ovulation utilizing gonadotropins and gonadotropin releasing hormone (GnRH) antagonists and IUI were divided to three groups based on the time from hCG injection to IUI: 36, 42, and 48 hours. Primary outcome was defined as the clinical pregnancy rate. Secondary outcomes comprised additional paramete...
Source: Systems Biology in Reproductive Medicine - August 18, 2014 Category: Reproduction Medicine Authors: Weiss A, Beck-Fruchter R, Lavee M, Geslevich Y, Golan J, Ermoshkin A, Shalev E Tags: Syst Biol Reprod Med Source Type: research

Is sperm hyaluronic acid binding ability predictive for clinical success of intracytoplasmic sperm injection: PICSI vs. ICSI?
e;r Z, Jakab A, Ujfalusi A, Oláh E Abstract Abstract Although intracytoplasmic sperm injection (ICSI) is now a widely-used technique, it is still of interest to improve our knowledge as to which is the best spermatozoon to be selected for ICSI. Infertile men have increased risks of producing aneuploid spermatozoa. Using hyaluronic acid (HA)-binding sperm selection may reduce the genetic risks such as chromosomal aberrations of offspring. In the present study we examined the clinical success of ICSI with HA-selected sperm ('physiologic' ICSI, PICSI) compared to conventional ICSI, as well as the necessity to ...
Source: Systems Biology in Reproductive Medicine - August 14, 2014 Category: Reproduction Medicine Authors: Mokánszki A, Tóthné EV, Bodnár B, Tándor Z, Molnár Z, Jakab A, Ujfalusi A, Oláh E Tags: Syst Biol Reprod Med Source Type: research

Different segregation patterns in five carriers due to a pericentric inversion of chromosome 1.
Abstract Abstract Pericentric inversion can produce recombinant gametes; however, meiotic segregation studies on the relationship between the frequency of recombinants and the inverted segment size are rare. Triple-color fluorescence in situ hybridization (FISH) was performed to analyze the meiotic behavior in five inv(1) carriers with different breakpoints. Recombination gametes were absent in Patient 1, whereas the percentages of the recombinants in Patients 2, 3, 4, and 5 were of 9.2%, 15.3%, 17.3%, and 40.9%, respectively. A significant difference was present for the frequencies of the recombinant spermatozoa ...
Source: Systems Biology in Reproductive Medicine - August 6, 2014 Category: Reproduction Medicine Authors: Luo Y, Xu C, Sun Y, Wang L, Chen S, Jin F Tags: Syst Biol Reprod Med Source Type: research

A comparison of sperm RNA-seq methods.
In this study, the following four commercially available RNA-seq amplification and library protocols for the preparation of low quantity/highly fragmented samples, SMARTer™ Ultra Low RNA (SU) for Illumina® Sequencing, SeqPlex RNA Amplification (SP), Ovation® RNA-Seq System V2 (OR), and Ovation® RNA-Seq Formalin Fixed Paraffin Embedded System (FFPES) were assessed using human sperm RNAs. Further investigation analyzed the effects on the end results of two different library preparation methods, Encore NGS Multiplex System I (Enc) and Ovation Ultralow Library Systems (UL), that appeared best suited to this t...
Source: Systems Biology in Reproductive Medicine - July 31, 2014 Category: Reproduction Medicine Authors: Mao S, Sendler E, Goodrich RJ, Hauser R, Krawetz SA Tags: Syst Biol Reprod Med Source Type: research

Polymorphisms of glutathione peroxidase 1 (GPX1 Pro198Leu) and catalase (CAT C-262T) in women with spontaneous abortion.
In conclusion, this study indicates that CAT -262T/T genotype confers less susceptibility to spontaneous abortion, while GPX1 Pro198Leu polymorphism may not be correlated with the disease. PMID: 25033027 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 17, 2014 Category: Reproduction Medicine Authors: Eskafi Sabet E, Salehi Z, Khodayari S, Sabouhi Zarafshan S, Zahiri Z Tags: Syst Biol Reprod Med Source Type: research

Comparative study of sperm washing and selection methods after cryopreservation and its influence on sperm subpopulational structure in a bovine model.
al CL Abstract Abstract The effect of different sperm washing-selection methods on sperm morphometric characteristics as a study to detect differences in the subpopulational structure has been carried out in detail in a bovine model. Cryopreserved sperm samples from 5 bulls were thawed, pooled, and processed by TALP-washing centrifugation method (TWCM), selective Percoll discontinuous density-gradient centrifugation method (PDGM), and self-migration swim-up separation method (SUMM). Live-dead assay (SYBR-14/ethidium homodimer-1), chlortetracycline assay (CTC), and sperm motility were assessed, and aliquots of sper...
Source: Systems Biology in Reproductive Medicine - July 9, 2014 Category: Reproduction Medicine Authors: García-Herreros M, Leal CL Tags: Syst Biol Reprod Med Source Type: research

XIAP protein is induced by placenta growth factor (PLGF) and decreased during preeclampsia in trophoblast cells.
Abstract Abstract Increased trophoblast apoptosis has been implicated in pregnancies complicated by fetal growth restriction and preeclampsia (EC). We investigated placenta growth factor (PLGF) signaling during trophoblast apoptosis in culture and X-linked inhibitor of apoptosis protein (XIAP) and apoptosis inducible factor (AIF) in the preeclamptic placenta at term was determined. Primary trophoblasts were isolated and serum starved to induce apoptosis. Placenta growth factor was added and apoptosis markers were determined. Term preeclamptic placentae were homogenized and the levels of XIAP and XAF1 protein were ...
Source: Systems Biology in Reproductive Medicine - July 8, 2014 Category: Reproduction Medicine Authors: Arroyo J, Price M, Straszewski-Chavez S, Torry RJ, Mor G, Torry DS Tags: Syst Biol Reprod Med Source Type: research

Fluoxetine induces changes in the testicle and testosterone in adult male rats exposed via placenta and lactation.
In conclusion, exposure to 20 mg/kg fluoxetine via the placenta and during lactation may change testosterone and testicular parameters important for sperm production and male fertility in adulthood. PMID: 24983959 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 1, 2014 Category: Reproduction Medicine Authors: Monteiro Filho WO, Torres SM, Amorim MJ, Andrade AJ, Morais RN, Tenorio BM, Silva Junior VA Tags: Syst Biol Reprod Med Source Type: research

Is the chronic use of Ferula harmonis to enhance mice erectile function effective and safe? A histopathological study.
This study aimed to investigate the impact of the chronic administration of F. hermonis root extract on the structure of the male mice reproductive organs and their fertility and to study the possible protective role of vitamin C. Sixty male albino mice were divided into 3 groups: the control, the experimental group that received F. hermonis root extract orally (6 mg/kg) for six weeks, and the treated group that received F. hermonis plus vitamin C for six weeks. Serum testosterone level and mice fertility were assessed. At the end of the experiment mice were sacrificed; testis, epididymis, and seminal vesicle were d...
Source: Systems Biology in Reproductive Medicine - May 28, 2014 Category: Reproduction Medicine Authors: Ayuob NN, Al-Harbi MS, Abdulhadi SS Tags: Syst Biol Reprod Med Source Type: research

Connexin 43 expression in Sprague-Dawley rat seminiferous epithelium after in utero exposure to flutamide.
This study explored the expression of connexin 43 (Cx43) in the testes of prepubertal Sprague-Dawley (SD) rats following in utero flutamide (Flu) exposure. Connexins constitute the major protein type in gap junctions. Connexin 43, the most prominent connexin family member expressed by testes, is localized at the base of seminiferous tubules in humans and rodents, and may be involved in fertility. Flutamide was injected subcutaneously into pregnant SD rats on gestational days 12-21 (25 mg/kg/day). Immunohistochemistry, Western blotting, and real-time PCR was used to investigate the distribution and the expression of ...
Source: Systems Biology in Reproductive Medicine - May 27, 2014 Category: Reproduction Medicine Authors: Cai C, Zhao D, Ma C, Zhang Y, Wu X, Wei G, He D Tags: Syst Biol Reprod Med Source Type: research

Recovery of ovary function impaired by chemotherapy using Chinese herbal medicine in a rat model.
In conclusion, Chinese herbal medicine showed a beneficial role in the recovery of ovary function in these rat models and has significant potential in the clinic. PMID: 24831605 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - May 15, 2014 Category: Reproduction Medicine Authors: Xia T, Fu Y, Gao H, Zhao Z, Zhao L, Han B Tags: Syst Biol Reprod Med Source Type: research

Mechanisms of oligozoospermia: an oxidative stress perspective.
Abstract Abstract Infertile patients presenting with poor semen concentration, motility, and morphology have significantly higher levels of reactive oxygen species (ROS). In this cross-sectional study, our goal was to: 1) determine how semen parameters such as concentration, motility, morphology, as well as ROS are altered in oligozoospermic men alone and those in combination with poor sperm concentration, motility, morphology, and compare these with three control groups; unproven donors, donors with proven fertility 2 years; 2) establish the cutoff, sensitivity, specificity for ROS, and see how it compared in the...
Source: Systems Biology in Reproductive Medicine - May 9, 2014 Category: Reproduction Medicine Authors: Agarwal A, Mulgund A, Sharma R, Sabanegh E Tags: Syst Biol Reprod Med Source Type: research

Successful pregnancy following assisted reproduction and transmyometrial embryo transfer in a patient with anatomical distortion of the cervical canal.
Abstract Abstract Presented is the case report of a patient noted to have gross distortion of the internal cervical canal during her attempt at embryo transfer following an in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI) procedure. Multiple attempts at cervical dilation were unsuccessful and the patient was ultimately treated by transmyometrial embryo transfer also known as the Towako method. She successfully achieved a singleton pregnancy and delivered at 41 weeks by primary cesarean section because of arrest of cervical dilation. Transmyometrial embryo transfer represents a viable option ...
Source: Systems Biology in Reproductive Medicine - May 5, 2014 Category: Reproduction Medicine Authors: Sullivan-Pyke CS, Kort DH, Sauer MV, Douglas NC Tags: Syst Biol Reprod Med Source Type: research

Effect of chocolate and Propolfenol on rabbit spermatogenesis and sperm quality following bacterial lipopolysaccharide treatment.
Abstract Abstract The aims of the study were to evaluate the effects of chocolate and propolis-enriched diets on rabbit spermatogenesis, sperm motility, and ultrastructure following bacterial lipopolysaccharide (LPS) treatment. Thirty-two New Zealand White rabbits were divided into four groups. The LPS-Propolfenol® group received propolis (500 mg/kg/day) in their diet for 15 days, while the LPS-chocolate group was fed 70% cacao chocolate (1 g/1 kg/day) for the same period. Following the diet treatments, rabbits in the LPS-Propolfenol® and LPS-chocolate groups, and an LPS group received...
Source: Systems Biology in Reproductive Medicine - April 30, 2014 Category: Reproduction Medicine Authors: Collodel G, Moretti E, Del Vecchio MT, Biagi M, Cardinali R, Mazzi L, Brecchia G, Maranesi M, Manca D, Castellini C Tags: Syst Biol Reprod Med Source Type: research

The role of mitochondrial aconitate (ACO2) in human sperm motility.
Abstract Abstract Deficiencies in tricarboxylic acid (TCA) cycle enzymes have been shown to cause a wide spectrum of human diseases, including malignancies and neurological and cardiac diseases. In mammalian spermatozoa mitochondria, the TCA cycle is known to be a crucial metabolic pathway that contributes to produce ATP. There is little known about the role and mechanism of mitochondrial aconitase (ACO2), which is an important regulatory enzyme of the TCA cycle, in asthenozoospermia. In the current study, immunofluorescence staining localized ACO2 to the human sperm mid-piece. By immunoblotting, we demonstrated t...
Source: Systems Biology in Reproductive Medicine - April 30, 2014 Category: Reproduction Medicine Authors: Tang M, Liu BJ, Wang SQ, Xu Y, Han P, Li PC, Wang ZJ, Song NH, Zhang W, Yin CJ Tags: Syst Biol Reprod Med Source Type: research

Protective effects of different antioxidants against cadmium induced oxidative damage in rat testis and prostate tissues.
Abstract Abstract The present study was performed to determine the effects of different antioxidants on testicular histopathology and oxidative damage induced by cadmium (Cd) in rat testis and prostate. Twenty five rats were equally divided into five groups (n = 5/group). The control group was injected subcutaneously with saline while the Cd alone treated group received a subcutaneous injection of 0.2 mg/kg CdCl2. Other groups were treated with sulphoraphane (25 µg/rat), vitamin E (75 mg/kg), and Ficus Religiosa plant extract (100 mg/kg) orally along with subcutaneou...
Source: Systems Biology in Reproductive Medicine - April 23, 2014 Category: Reproduction Medicine Authors: Jahan S, Zahra A, Irum U, Iftikhar N, Ullah H Tags: Syst Biol Reprod Med Source Type: research

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function.
Abstract Abstract Despite extensive research carried out for optimization and commercialization of sperm cryopreservation media, percentage of motility and viability remain low following cryopreservation. These adverse effects have been partially ascribed to reactive oxygen species (ROS) production during cryopreservation. Therefore, we proposed that addition of a cell permeable antioxidant like Tempol, with superoxide dismutase (SOD) mimetic action, may overcome these effects in an optimized commercially available cryo-protective medium. Therefore, semen samples were cryopreserved in the presence or absence of Te...
Source: Systems Biology in Reproductive Medicine - April 7, 2014 Category: Reproduction Medicine Authors: Bateni Z, Azadi L, Tavalaee M, Kiani-Esfahani A, Fazilati M, Nasr-Esfahani MH Tags: Syst Biol Reprod Med Source Type: research

Effects of occupational exposure - is there a link between exposure based on an occupational questionnaire and semen quality?
This study showed associations between self-reported occupational exposures and impaired semen parameters. The occupational exposure questionnaire may be useful in clinical practice for patients and physicians to identify the work factors associated with poorer semen quality. PMID: 24702586 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - April 7, 2014 Category: Reproduction Medicine Authors: Jurewicz J, Radwan M, Sobala W, Radwan P, Bochenek M, Hanke W Tags: Syst Biol Reprod Med Source Type: research

Effect of various commercial buffers on sperm viability and capacitation.
din L Abstract Abstract A wide variety of sperm preparation protocols are currently available for assisted conception. They include density gradient separation and washing methods. Both aim at isolating and capacitating as much motile sperm as possible for subsequent oocyte fertilization. The aim of this study was to examine the effects of four commercial sperm washing buffers on sperm viability and capacitation. Semen samples from 48 healthy donors (normal values of sperm count, motility, morphology, and volume) were analyzed. After separation (density gradient 40/80%), sperm were incubated in various buffers the...
Source: Systems Biology in Reproductive Medicine - March 27, 2014 Category: Reproduction Medicine Authors: Andrisani A, Donà G, Ambrosini G, Bonanni G, Bragadin M, Cosmi E, Clari G, Armanini D, Bordin L Tags: Syst Biol Reprod Med Source Type: research