Current status of sperm functional genomics and its diagnostic potential of fertility in bovine (Bos taurus).
Abstract With artificial insemination (AI) and other precision dependent assisted reproductive technologies (ART) being followed in large scale in human and animal reproduction, assessing semen quality and fertilizability is under continuous scrutiny. Various tests have been developed to predict semen quality, but so far no single, highly reliable test is available. In this regard, transcriptomic profiling of spermatozoa assumes significance as it carries the information about spermatogenesis, sperm function, and paternal roles in post-fertilization events. Human spermatozoal transcriptome profiling has been carri...
Source: Systems Biology in Reproductive Medicine - March 14, 2018 Category: Reproduction Medicine Authors: Selvaraju S, Parthipan S, Somashekar L, Binsila BK, Kolte AP, Arangasamy A, Ravindra JP, Krawetz SA Tags: Syst Biol Reprod Med Source Type: research

Triiodothyronine stimulates VEGF expression and secretion via steroids and HIF-1 α in murine Leydig cells.
Triiodothyronine stimulates VEGF expression and secretion via steroids and HIF-1α in murine Leydig cells. Syst Biol Reprod Med. 2018 Feb 08;:1-11 Authors: Dhole B, Gupta S, Venugopal SK, Kumar A Abstract Leydig cells are the principal steroidogenic cells of the testis. Leydig cells also secrete a number of growth factors including vascular endothelial growth factor (VEGF) which has been shown to regulate both testicular steroidogenesis and spermatogenesis. The thyroid hormone, T3, is known to stimulate steroidogenesis in Leydig cells. T3 has also been shown to stimulate VEGF production in a vari...
Source: Systems Biology in Reproductive Medicine - February 8, 2018 Category: Reproduction Medicine Authors: Dhole B, Gupta S, Venugopal SK, Kumar A Tags: Syst Biol Reprod Med Source Type: research

Evaluation of the impact of sperm morphology on embryo aneuploidy rates in a donor oocyte program.
This study investigates the correlation between sperm morphology and the incidence of embryo aneuploidy in an oocyte donation program. A total of 1,165 embryos from 103 patients have been analyzed by fluorescent in situ hybridization (FISH) for numerical abnormalities in chromosome numbers 13, 18, 21, X, and Y. Data has been evaluated in five groups according to sperm morphology, which has been assessed according to the Kruger's strict criteria. The results did not show any difference in paternal (p = 0.878), maternal (p = 0.873), and donor ages (p = 0.871), sperm counts (p = 0.782) ...
Source: Systems Biology in Reproductive Medicine - January 20, 2018 Category: Reproduction Medicine Authors: Coban O, Serdarogullari M, Onar Sekerci Z, Bilgin EM, Serakinci N Tags: Syst Biol Reprod Med Source Type: research

Effects of different open cryo-carriers on embryo survival and clinical outcome in frozen embryo transfer cycle patients.
Abstract The purpose of this study was to compare the efficacy of different open cryo-carriers: the CryoloopTM, CryotopTM, and CryoleafTM, in embryo survival and clinical outcome in patients with frozen embryo transfer (FET) cycle. We analyzed the embryo survival rate and clinical outcome in 325 patients of 348 FET cycles vitrified with the CryoloopTM (160 cycles), CryotopTM (105 cycles), or CryoleafTM (83 cycles). No significant differences were observed in embryo survival rate (98.8% vs. 100% vs. 97.7%, p > 0.05), HCG positive rate (58.8% vs. 63.8% vs. 57.8%, p > 0.05), biochemical preg...
Source: Systems Biology in Reproductive Medicine - January 20, 2018 Category: Reproduction Medicine Authors: Chu D, Fu L, Zhou W, Li Y Tags: Syst Biol Reprod Med Source Type: research

The role of the Fas/FasL signaling pathway in environmental toxicant-induced testicular cell apoptosis: An update.
Abstract The Fas/FasL signaling pathway is one of the major pathways that regulate apoptosis. Increasing studies have shown that the activation of the Fas/FasL signaling pathway is closely associated with testicular cell apoptosis. However, the mechanism involved is still unclear. We discuss recent findings regarding the molecular mechanisms by which environmental toxicants induce testicular pathology via Fas/FasL signaling. These findings suggest that Fas/FasL signaling is employed to impact the sensitivity (a response to external factors) of germ cells, disrupt steroidogenic hormone and cytokine metabolism media...
Source: Systems Biology in Reproductive Medicine - January 4, 2018 Category: Reproduction Medicine Authors: Wang M, Su P Tags: Syst Biol Reprod Med Source Type: research

The role of the Fas/FasL signaling pathway in environmental toxicant-induced testicular cell apoptosis: An update
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Source: Systems Biology in Reproductive Medicine - January 4, 2018 Category: Sexual Medicine Authors: Mei Wang Ping Su Source Type: research

Cleavage-stage embryo micromanipulation in the clinical setting.
Abstract Embryo micromanipulation was developed after introduction of microinjection to overcome infertility. Embryo micromanipulation may be performed at any embryo stage from pronuclear to blastocyst. The technique started out as basic and turned out to be increasingly more complex. Embryo micromanipulation at the cleavage-stage includes a wide range of techniques, from opening the zona pellucida in order to improve the chance of implantation, to removing detrimental components from the embryo to enhance embryo development or blastomeres for preimplantation genetic diagnosis and embryo splitting. Evaluating the ...
Source: Systems Biology in Reproductive Medicine - January 3, 2018 Category: Reproduction Medicine Authors: Halvaei I, Ghazali S, Nottola SA, Khalili MA Tags: Syst Biol Reprod Med Source Type: research

Cleavage-stage embryo micromanipulation in the clinical setting
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - January 3, 2018 Category: Sexual Medicine Authors: Iman Halvaei Shahin Ghazali Stefania A. Nottola Mohammad Ali Khalili Source Type: research

Does genome organization matter in spermatozoa? A refined hypothesis to awaken the silent vessel
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Source: Systems Biology in Reproductive Medicine - January 2, 2018 Category: Sexual Medicine Authors: Dimitrios Ioannou Helen G. Tempest Source Type: research

Bayesian analysis improves pulse secretion characterization in reproductive hormones
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 30, 2017 Category: Sexual Medicine Authors: Huayu Liu Alex J. Polotsky Gary K. Grunwald Nichole E. Carlson Source Type: research

Bayesian analysis improves pulse secretion characterization in reproductive hormones.
The objective of this study was to extensively validate a Bayesian analysis approach for analyzing pulsatile hormones in settings that occur in reproductive studies. An investigative approach was chosen so that clinical research teams will have the knowledge to adopt this newer analysis approach in practice. Three experimental conditions were investigated: luteinizing hormone (LH) profiles in ovariectomized ewes (N=6; high signal-to-noise setting), LH profiles in young ovulating women (N=12; lower signal-to-noise setting), and computer-simulated scenarios (N=200). For each experimental condition, differences in luteinizing...
Source: Systems Biology in Reproductive Medicine - December 29, 2017 Category: Reproduction Medicine Authors: Liu H, Polotsky AJ, Grunwald GK, Carlson NE Tags: Syst Biol Reprod Med Source Type: research

The first decade of Systems Biology in Reproductive Medicine
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 14, 2017 Category: Sexual Medicine Source Type: research

The first decade of Systems Biology in Reproductive Medicine.
Authors: PMID: 29239675 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 14, 2017 Category: Reproduction Medicine Tags: Syst Biol Reprod Med Source Type: research

The first decade of Systems Biology in Reproductive Medicine
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 14, 2017 Category: Sexual Medicine Source Type: research

Effect of the induction of transgenerational obesity on maternal-fetal parameters
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 11, 2017 Category: Sexual Medicine Authors: Thaigra Sousa Soares Ana Paula Andreolla Carolina Abreu Miranda Eduardo Kl öppel Luhara Silva Rodrigues Rafaianne Queiroz Moraes-Souza D ébora Cristina Damasceno Gustavo Tadeu Volpato Kleber Eduardo Campos Source Type: research

Sex hormone binding globulin - an important biomarker for predicting PCOS risk: A systematic review and meta-analysis
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 11, 2017 Category: Sexual Medicine Authors: Ritu Deswal Arun Yadav Amita Suneja Dang Source Type: research

Sex hormone binding globulin - an important biomarker for predicting PCOS risk: A systematic review and meta-analysis.
Abstract Sex hormone-binding globulin (SHBG) is a glycoprotein which regulates bioavailability of sex steroid hormones. Interest in SHBG has escalated in recent years because of its inverse association with polycystic ovary syndrome (PCOS), obesity, insulin resistance, metabolic syndrome, and diabetes type II. This meta-analysis was performed to examine the associations of SHBG with PCOS and to correlate serum SHBG levels with various PCOS associated endocrine and metabolic dysregulation as well as to determine the effects of various therapeutic agents on serum SHBG levels in PCOS patients in order to assess the t...
Source: Systems Biology in Reproductive Medicine - December 11, 2017 Category: Reproduction Medicine Authors: Deswal R, Yadav A, Dang AS Tags: Syst Biol Reprod Med Source Type: research

Effect of the induction of transgenerational obesity on maternal-fetal parameters.
KE Abstract Maternal obesity can cause complications for both women and their offspring for generations. Therefore, we intended to verify the repercussions of induction of transgenerational obesity on biochemical parameters, reproductive performance, and congenital anomaly frequency in Wistar rats. Female rats were used from successive generations. The female rats of parental generation (F0, n=10) were mated to obtain their offspring (F1 generation). F1 female rats received a monosodium glutamate (MSG) solution to induce obesity (n=07) or vehicle (control, n=06) during the neonatal period. These adult female rats...
Source: Systems Biology in Reproductive Medicine - December 11, 2017 Category: Reproduction Medicine Authors: Soares TS, Andreolla AP, Miranda CA, Klöppel E, Rodrigues LS, Moraes-Souza RQ, Damasceno DC, Volpato GT, Campos KE Tags: Syst Biol Reprod Med Source Type: research

Effect of the induction of transgenerational obesity on maternal-fetal parameters
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 11, 2017 Category: Sexual Medicine Authors: Thaigra Sousa Soares Ana Paula Andreolla Carolina Abreu Miranda Eduardo Kl öppel Luhara Silva Rodrigues Rafaianne Queiroz Moraes-Souza D ébora Cristina Damasceno Gustavo Tadeu Volpato Kleber Eduardo Campos Source Type: research

Sex hormone binding globulin - an important biomarker for predicting PCOS risk: A systematic review and meta-analysis
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 11, 2017 Category: Sexual Medicine Authors: Ritu Deswal Arun Yadav Amita Suneja Dang Source Type: research

Regulation of follicle growth through hormonal factors and mechanical cues mediated by Hippo signaling pathway
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 9, 2017 Category: Sexual Medicine Authors: Ikko Kawashima Kazuhiro Kawamura Source Type: research

Regulation of follicle growth through hormonal factors and mechanical cues mediated by Hippo signaling pathway.
Abstract The ovary is an interesting organ that shows major structural changes within a short period of time during each reproductive cycle. Follicle development is controlled by local paracrine and systemic endocrine factors. Many hormonal and molecular analyses have been conducted to find the mechanisms underlying structural changes in ovaries, However, exact mechanisms still remain to be determined. Recent development of mechanobiology facilitates the understanding on the contribution of physical forces and changes in the mechanical properties of cells and tissues to physiology and pathophysiology. The Hippo si...
Source: Systems Biology in Reproductive Medicine - December 9, 2017 Category: Reproduction Medicine Authors: Kawashima I, Kawamura K Tags: Syst Biol Reprod Med Source Type: research

Regulation of follicle growth through hormonal factors and mechanical cues mediated by Hippo signaling pathway
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 9, 2017 Category: Sexual Medicine Authors: Ikko Kawashima Kazuhiro Kawamura Source Type: research

Sperm RNA elements as markers of health
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 4, 2017 Category: Sexual Medicine Authors: Rayanne B. Burl Stephanie Clough Edward Sendler Molly Estill Stephen A. Krawetz Source Type: research

Sperm RNA elements as markers of health.
Abstract Idiopathic infertility, an etiology not identified as part of standard clinical assessment, represents approximately 20% of all infertility cases. Current male infertility diagnosis focuses on the concentration, motility, and morphology of spermatozoa. This is of limited value when predicting birth success and of limited utility when selecting the optimum treatment. At fertilization, spermatozoa provide their genomic contribution, as well as a set of RNAs and proteins that have distinct roles in development. The potential of spermatozoal RNAs to be used as a prognostic of live birth has been shown [Jodar ...
Source: Systems Biology in Reproductive Medicine - December 3, 2017 Category: Reproduction Medicine Authors: Burl RB, Clough S, Sendler E, Estill M, Krawetz SA Tags: Syst Biol Reprod Med Source Type: research

Initial germ cell to somatic cell ratio impacts the efficiency of SSC expansion in vitro
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Source: Systems Biology in Reproductive Medicine - December 1, 2017 Category: Sexual Medicine Authors: Itai Gat Leila Maghen Melissa Filice Shlomit Kenigsberg Brandon Wyse Khaled Zohni Peter Saraz Andr ée Gauthier Fisher Clifford Librach Source Type: research

Initial germ cell to somatic cell ratio impacts the efficiency of SSC expansion in vitro.
Abstract Spermatogonial Stem Cell (SSC) expansion in vitro remains a major challenge in efforts to preserve fertility among pubertal cancer survivor boys. The current study focused on innovative approaches to optimize SSC expansion. Six- to eight-week-old CD-1 murine testicular samples were harvested by mechanical and enzymatic digestion. Cell suspensions were incubated for differential plating (DP). After DP, we established two experiments comparing single vs. repetitive DP (S-DP and R-DP, respectively) until passage 2 (P2) completion. Each experiment included a set of cultures consisting of 5 floating-to-attache...
Source: Systems Biology in Reproductive Medicine - December 1, 2017 Category: Reproduction Medicine Authors: Gat I, Maghen L, Filice M, Kenigsberg S, Wyse B, Zohni K, Saraz P, Fisher AG, Librach C Tags: Syst Biol Reprod Med Source Type: research

Initial germ cell to somatic cell ratio impacts the efficiency of SSC expansion in vitro
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 1, 2017 Category: Sexual Medicine Authors: Itai Gat Leila Maghen Melissa Filice Shlomit Kenigsberg Brandon Wyse Khaled Zohni Peter Saraz Andr ée Gauthier Fisher Clifford Librach Source Type: research

2017 Systems Biology in Reproductive Medicine selected highlights.
Authors: PMID: 29182398 [PubMed - in process] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 30, 2017 Category: Reproduction Medicine Tags: Syst Biol Reprod Med Source Type: research

The effect of repeated light-dark shifts on uterine receptivity and early gestation in mice undergoing embryo transfer
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 30, 2017 Category: Sexual Medicine Authors: Cathy A. Goldstein Louise M. O ’Brien Ingrid L. Bergin Thomas L. Saunders Source Type: research

2017 Systems Biology in Reproductive Medicine selected highlights
Volume 63, Issue 6, December 2017, Page 405-407 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 28, 2017 Category: Sexual Medicine Source Type: research

Anogenital distance and variability in semen parameters
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 27, 2017 Category: Sexual Medicine Authors: Jos é J. López-Espín Consuelo P érez-Palazón Ana B. Maldonado-C árceles Jos é D. Román-Arias Jaime Mendiola Alberto M. Torres-Cantero Source Type: research

Anogenital distance and variability in semen parameters.
s JD, Mendiola J, Torres-Cantero AM Abstract The purpose of this study was to analyze whether the anogenital distance (AGD) was associated with variability in semen parameters. Semen parameters analyzed following the WHO guidelines and sperm DNA fragmentation were evaluated in 160 semen samples obtained over a period of a year from 16 healthy male volunteers. Two types of AGD measurements from the anus to the rear base of the scrotum (AGDAS) and to the cephalic insertion of the penis (AGDAP) were taken in each individual. The association between AGDs and semen parameters were studied using three statistical tools:...
Source: Systems Biology in Reproductive Medicine - November 27, 2017 Category: Reproduction Medicine Authors: López-Espín JJ, Pérez-Palazón C, Maldonado-Cárceles AB, Román-Arias JD, Mendiola J, Torres-Cantero AM Tags: Syst Biol Reprod Med Source Type: research

Anogenital distance and variability in semen parameters
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 27, 2017 Category: Sexual Medicine Authors: Jos é J. López-Espín Consuelo P érez-Palazón Ana B. Maldonado-C árceles Jos é D. Román-Arias Jaime Mendiola Alberto M. Torres-Cantero Source Type: research

A comparative evaluation of migration sedimentation method for sperm preparation
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 21, 2017 Category: Sexual Medicine Authors: Sevil Kiratli Mehmet Yuncu Kenan Kose Sinan Ozkavukcu Source Type: research

Experimental diabetes impairs maternal reproductive performance in pregnant Wistar rats and their offspring
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 21, 2017 Category: Sexual Medicine Authors: Leticia Bequer Tahiry G ómez Jos é L. Molina Alain Álvarez Claudia Chaviano Sonia Clap és Source Type: research

A comparative evaluation of migration sedimentation method for sperm preparation
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 21, 2017 Category: Sexual Medicine Authors: Sevil Kiratli Mehmet Yuncu Kenan Kose Sinan Ozkavukcu Source Type: research

Experimental diabetes impairs maternal reproductive performance in pregnant Wistar rats and their offspring
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 21, 2017 Category: Sexual Medicine Authors: Leticia Bequer Tahiry G ómez Jos é L. Molina Alain Álvarez Claudia Chaviano Sonia Clap és Source Type: research

Experimental diabetes impairs maternal reproductive performance in pregnant Wistar rats and their offspring.
, Clapés S Abstract The aim of this study was to determine the effect of mild hyperglycemia on metabolism during pregnancy, the maternal reproductive performance, and the characteristics of the offspring in neonatal mild diabetic-induced Wistar rats. The experimental diabetes model was generated by neonatal streptozotocin administration (100 mg of streptozotocin/Kg bw/sc) in female Wistar rats. At adulthood, the control and diabetic group were mated. At the 20th day of gestation, a maternal and fetal blood sample were collected for biochemical measurement. The maternal livers, fetal livers, and placenta wer...
Source: Systems Biology in Reproductive Medicine - November 20, 2017 Category: Reproduction Medicine Authors: Bequer L, Gómez T, Molina JL, Álvarez A, Chaviano C, Clapés S Tags: Syst Biol Reprod Med Source Type: research

A comparative evaluation of migration sedimentation method for sperm preparation.
Abstract The effectiveness of semen preparation using the migration-sedimentation (MS) method was evaluated, and compared to density gradient centrifuge and swim-up combination (DGC+SU). Sperm selection using MS is based on motility, thus, deleterious effects for which centrifugation has been blamed, are believed to be avoided. Normozoospermic male patients who had more than 10% forward progressive motile sperm in their ejaculate were included in the study. Spermatozoa selected by two different methods were investigated and compared according to sperm motility, concentration, morphology, vitality, DNA fragmentatio...
Source: Systems Biology in Reproductive Medicine - November 20, 2017 Category: Reproduction Medicine Authors: Kiratli S, Yuncu M, Kose K, Ozkavukcu S Tags: Syst Biol Reprod Med Source Type: research

Aging deteriorates quality of sperm produced by male mice with partial Yq deletion
Volume 63, Issue 6, December 2017, Page 360-363 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 3, 2017 Category: Sexual Medicine Authors: Katarzyna Kotarska Andrzej Doniec Magdalena Bednarska Zbigniew Pola ński J ózefa Styrna Source Type: research

Regulatory T cells improve pregnancy rate in RIF patients after additional IVIG treatment
Volume 63, Issue 6, December 2017, Page 350-359 . (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 3, 2017 Category: Sexual Medicine Authors: Majid Ahmadi Samaneh Abdolmohammadi-Vahid Mahnaz Ghaebi Leili Aghebati-Maleki Sanam Dolati Laya Farzadi Aliyeh Ghasemzadeh Kobra Hamdi Vahid Younesi Mohammad Nouri Mehdi Yousefi Source Type: research

Regulatory T cells improve pregnancy rate in RIF patients after additional IVIG treatment.
In conclusion, IVIG therapy may be a good choice in the treatment of implantation failure in RIF women especially with an immunologic basis, and may improve the implantation and pregnancy rate by affecting immunoregulatory mechanisms such as Tregs. ABBREVIATIONS: RIF: repeated implantation failure; IVIG: intravenous immunoglobulin; Th17: T helper 17; Treg: T regulatory; NK cells: natural killer cells; PCR: polymerase chain reaction; ELISA: enzyme-linked immunosorbent assay; RORγt: RAR-related orphan receptor gamma; Foxp3: forkhead box protein P3; IL-17: interleukin-17; LMWH: low-molecular weight heparin; dNK: de...
Source: Systems Biology in Reproductive Medicine - November 3, 2017 Category: Reproduction Medicine Authors: Ahmadi M, Abdolmohammadi-Vahid S, Ghaebi M, Aghebati-Maleki L, Dolati S, Farzadi L, Ghasemzadeh A, Hamdi K, Younesi V, Nouri M, Yousefi M Tags: Syst Biol Reprod Med Source Type: research

Aging deteriorates quality of sperm produced by male mice with partial Yq deletion.
Abstract The aim of the study was to assess the cumulative effects of aging and Y-chromosome long arm deletion on sperm quality parameters. Motility, mitochondrial activity, and head morphology were evaluated for sperm of 3- and 12-month-old males from B10.BR-Y(del) and B10.BR congenic mouse strains. The study revealed that quality and fertilizing potential of sperm produced by younger and older B10.BR males persist on similar levels, but worsen significantly with age of B10.BR-Y(del) males. The findings imply that partial Yq deletions might be more harmful for spermiogenesis in advancing age and may be applicable...
Source: Systems Biology in Reproductive Medicine - November 3, 2017 Category: Reproduction Medicine Authors: Kotarska K, Doniec A, Bednarska M, Polański Z, Styrna J Tags: Syst Biol Reprod Med Source Type: research

Aging deteriorates quality of sperm produced by male mice with partial Yq deletion
. (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 3, 2017 Category: Sexual Medicine Authors: Katarzyna Kotarska Andrzej Doniec Magdalena Bednarska Zbigniew Pola ński J ózefa Styrna Source Type: research