Nonprofessional phagocytosis in trophectoderm cells of human preimplantation blastocysts.
In this study, we determined the phagocytosis potential in human trophectoderm cells of blastocysts prior to implantation. Fluorescent microspheres were used as markers for phagocytic analysis under transmission electron microscope (TEM) and fluorescence microscopy. Phagocytosis of 1 μm fluorescent microspheres was observed in most (9/11) day-6 and even some (2/9) day-5 blastocysts. More effective phagocytosis occurred in blastocysts at the morula-blastocyst stage of day-6. Furthermore, we observed an increased trend of phagocytic acitivities in polar trophectoderm. Our findings indicated phagocytic ability exists in hu...
Source: Systems Biology in Reproductive Medicine - June 16, 2016 Category: Reproduction Medicine Authors: Li Y, Xu J, Zhou CQ, Zhang CL, Zhuang GL Tags: Syst Biol Reprod Med Source Type: research

The impact of supervised weight loss and intentional weight regain on sex hormone binding globulin and testosterone in premenopausal women
Volume 62, Issue 4, August 2016, pages 283-289 10.1080/19396368.2016.1177619 Mira Aubuchon (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - May 17, 2016 Category: Sexual Medicine Authors: Mira Aubuchon Ying Liu Gregory F. Petroski Tom R. Thomas Alex J. Polotsky Source Type: research

The impact of supervised weight loss and intentional weight regain on sex hormone binding globulin and testosterone in premenopausal women.
Abstract What is the impact of intentional weight loss and regain on serum androgens in women? We conducted an ancillary analysis of prospectively collected samples from a randomized controlled trial. The trial involved supervised 10% weight loss (8.5 kg on average) with diet and exercise over 4-6 months followed by supervised intentional regain of 50% of the lost weight (4.6 kg on average) over 4-6 months. Participants were randomized prior to the partial weight regain component to either continuation or cessation of endurance exercise. Analytic sample included 30 obese premenopausal women (mean age of 40 ±...
Source: Systems Biology in Reproductive Medicine - May 17, 2016 Category: Reproduction Medicine Authors: Aubuchon M, Liu Y, Petroski GF, Thomas TR, Polotsky AJ Tags: Syst Biol Reprod Med Source Type: research

Corpus luteum as a novel target of weight changes that contribute to impaired female reproductive physiology and function
Volume 62, Issue 4, August 2016, pages 227-242 10.3109/19396368.2016.1173743 Satu Kuokkanen (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - May 16, 2016 Category: Sexual Medicine Authors: Satu Kuokkanen Alex J. Polotsky Justin Chosich Andrew P. Bradford Anna Jasinska Tzu Phang Nanette Santoro Susan E. Appt Source Type: research

Corpus luteum as a novel target of weight changes that contribute to impaired female reproductive physiology and function.
Abstract Obesity and malnutrition are associated with decreased fecundity in women. Impaired reproductive capacity in obese women is often attributed to anovulation. However, obese women with ovulatory cycles also have reduced fertility, but the etiology of their impaired reproduction is only partially understood. Accumulating evidence suggests that obesity directly impairs oocyte and embryo quality as well as endometrial receptivity. In obese women, urinary progesterone metabolite excretion is decreased, but in excess of what can be explained by suppressed gonadotropin secretion, suggesting that apart from its ce...
Source: Systems Biology in Reproductive Medicine - May 16, 2016 Category: Reproduction Medicine Authors: Kuokkanen S, Polotsky AJ, Chosich J, Bradford AP, Jasinska A, Phang T, Santoro N, Appt SE Tags: Syst Biol Reprod Med Source Type: research

Gonadotrophin-releasing hormone agonist protocol of controlled ovarian hyperstimulation as an efficient treatment in Bologna-defined poor ovarian responders
Volume 62, Issue 4, August 2016, pages 290-296 10.3109/19396368.2016.1170229 Martin Stimpfel (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - April 24, 2016 Category: Sexual Medicine Authors: Martin Stimpfel Eda Vrta čnik-Bokal Barbara Pozlep Jerneja Kmecl Irma Virant-Klun Source Type: research

Gonadotrophin-releasing hormone agonist protocol of controlled ovarian hyperstimulation as an efficient treatment in Bologna-defined poor ovarian responders.
Abstract In this retrospective study the outcomes of two protocols of controlled ovarian hyperstimulation and natural cycle in poor ovarian responders defined according to the Bologna criteria were compared to elucidate which approach is more suitable for the treatment of these patients. We comparatively analyzed 142 cycles of GnRH antagonist (GnRH-ant) protocol, 53 cycles of GnRH agonist (GnRH-a) protocol, and 36 natural cycles. The mean number of oocytes (2.8±1.8) and embryos (1.6±1.2) per aspiration was significantly higher in GnRH-a protocol in comparison to GnRH-ant protocol and natural cycle, b...
Source: Systems Biology in Reproductive Medicine - April 24, 2016 Category: Reproduction Medicine Authors: Stimpfel M, Vrtačnik-Bokal E, Pozlep B, Kmecl J, Virant-Klun I Tags: Syst Biol Reprod Med Source Type: research

Anti-Müllerian hormone in serum and seminal plasma in comparison with other male fertility parameters.
Anti-Müllerian hormone in serum and seminal plasma in comparison with other male fertility parameters. Syst Biol Reprod Med. 2016 Apr 25;:1-4 Authors: Kucera R, Ulcova-Gallova Z, Windrichova J, Losan P, Topolcan O Abstract Anti-Müllerian hormone (AMH) is a factor most associated with female fertility and especially with the ovarian reserve. AMH is also used as a parameter of fertility in men as it arises from the epithelium of the seminiferous tubules that contain Sertoli cells which produce the AMH. To investigate the relationship between AMH production and sperm related parameters we compa...
Source: Systems Biology in Reproductive Medicine - April 24, 2016 Category: Reproduction Medicine Authors: Kucera R, Ulcova-Gallova Z, Windrichova J, Losan P, Topolcan O Tags: Syst Biol Reprod Med Source Type: research

Expression pattern of G protein‑coupled estrogen receptor 1 (GPER) in human cumulus granulosa cells (CGCs) of patients with PCOS.
Abstract Estradiol mediates its actions by binding to classical nuclear receptors, estrogen receptor α (ER-α) and estrogen receptor β (ER-β), and the non-classical G protein-coupled estrogen receptor 1(GPER). Several gene knockdown models have shown the importance of the receptors for growth of the oocyte and for ovulation. The aim of our study was to identify the pattern of GPER expression in human cumulus granulosa cells (CGCs) from ovarian follicles at different stages of oocyte maturation, and the differences of GPER expression between polycystic ovary syndrome (PCOS) patients and non-PCO...
Source: Systems Biology in Reproductive Medicine - April 24, 2016 Category: Reproduction Medicine Authors: Zang L, Zhang Q, Zhou Y, Zhao Y, Lu L, Jiang Z, Peng Z, Zou S Tags: Syst Biol Reprod Med Source Type: research

Microfluidics: The future of microdissection TESE?
Abstract Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic techno...
Source: Systems Biology in Reproductive Medicine - April 21, 2016 Category: Reproduction Medicine Authors: Samuel R, Badamjav O, Murphy KE, Patel DP, Son J, Gale BK, Carrell DT, Hotaling JM Tags: Syst Biol Reprod Med Source Type: research

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesis in vitro but has no effect on embryo development.
Åkerud H Abstract Histidine-rich glycoprotein (HRG) is an abundant plasma protein involved in multiple biological processes including immunology, vascularisation, and coagulation. These processes are of importance in regulating embryo development and implantation. A specific polymorphism in the HRG gene, HRG C633T, has an impact on various aspects of fertility, such as oocyte quality, endometrial receptivity, and possibly the capacity of the embryo itself to implant. To further examine the potential role of the HRG C633T polymorphism in regulating endometrial angiogenesis and on embryo development, two HRG p...
Source: Systems Biology in Reproductive Medicine - March 29, 2016 Category: Reproduction Medicine Authors: Lindgren KE, Hreinsson J, Helmestam M, Wånggren K, Poromaa IS, Kårehed K, Åkerud H Tags: Syst Biol Reprod Med Source Type: research

The importance of transmission electron microscopy analysis of spermatozoa: Diagnostic applications and basic research.
Abstract This review is aimed at discussing the role of ultrastructural studies on human spermatozoa and evaluating transmission electron microscopy as a diagnostic tool that can complete andrology protocols. It is clear that morphological sperm defects may explain decreased fertilizing potential and acquire particular value in the field of male infertility. Electron microscopy is the best method to identify systematic or monomorphic and non-systematic or polymorphic sperm defects. The systematic defects are characterized by a particular anomaly that affects the vast majority of spermatozoa in a semen sample, wher...
Source: Systems Biology in Reproductive Medicine - March 16, 2016 Category: Reproduction Medicine Authors: Moretti E, Sutera G, Collodel G Tags: Syst Biol Reprod Med Source Type: research

Association of G22A and A4223C ADA1 gene polymorphisms and ADA activity with PCOS.
This study investigates serum total ADA activity (tADA), ADA1 and ADA2 isoenzyme activities, and genotype and allele frequencies of G22A and A4223C polymorphisms in healthy and PCOS women. In this case-control study 200 PCOS patients and 200 healthy women were enrolled. Genomic DNA was extracted from whole blood and the PCR-RFLP technique was used to determine the G22A and A4223C variants. The genotype frequencies were calculated and the association between polymorphic genotypes and enzyme activities were determined. tADA activity was significantly lower in the PCOS group compared with the control group (27.76±6.0 v...
Source: Systems Biology in Reproductive Medicine - March 15, 2016 Category: Reproduction Medicine Authors: Salehabadi M, Farimani M, Tavilani H, Ghorbani M, Poormonsefi F, Poorolajal J, Shafiei G, Ghasemkhani N, Khodadadi I Tags: Syst Biol Reprod Med Source Type: research

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss.
Abstract Contribution from a defective paternal genome has been attributed to be an important cause for spontaneous recurrent pregnancy loss (RPL). Increased oxidative stress results in decreased detoxification and is a cause for damage to chromatin, proteins, and membrane lipids. The present study aimed to explore if there is a significant relationship between retained histones due to defective packaging of DNA in spermatozoa and oxidative stress. RPL patients (n=16) with a history of ≥2 embryo losses before the 20th week of gestation and no female factor abnormality, and fertile healthy volunteers (n=20) as c...
Source: Systems Biology in Reproductive Medicine - March 15, 2016 Category: Reproduction Medicine Authors: Mohanty G, Swain N, Goswami C, Kar S, Samanta L Tags: Syst Biol Reprod Med Source Type: research

A rapidly progressive defective spermatogenesis in a Mexican family affected by spino-bulbar muscular atrophy.
In this report we describe a Mexican family with three affected brothers with primary infertility caused by a progressive impairment of spermatogenesis leading to azoospermia before 40 years of age. They presented common features associated to patients affected by SMBA, such as gynecomastia, high level of CPK, muscle cramps, fasciculations, muscle wastage, and impaired swallowing. Two intracytoplasmic sperm injection (ICSI) cycles were performed in one of the patients resulting in fertilization failure. Molecular analysis of AR gene exon 1 revealed 54 CAG repeats in DNA extracted from leukocytes in affected patients and 22...
Source: Systems Biology in Reproductive Medicine - February 22, 2016 Category: Reproduction Medicine Authors: Piña-Aguilar RE, Regalado-Hernández MÁ, Moreno-García JD, Buentello-Volante B, Chacón-Camacho OF, Gallegos-Rivas MC, Kazakova E, Santillán-Hernández Y, Zenteno JC Tags: Syst Biol Reprod Med Source Type: research

Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes.
Abstract Epigenetic abnormalities and abnormal chromatin structure in sperm may lead to male infertility. Protamine deficiency is among the disorders of chromatin structure in sperm. The study of epigenetic changes in male pronuclei is necessary since abnormal sperm is sometimes used to create embryos using assisted reproductive techniques. The present study was carried out to compare epigenetic global marks in male pronuclei derived from normal and protamine deficient sperm cells. To do so, interspecies fertilization was used to obtain the male pronucleus. Normal and protamine deficient sperm cells, which were id...
Source: Systems Biology in Reproductive Medicine - February 22, 2016 Category: Reproduction Medicine Authors: Rajabi H, Mohseni-Kouchesfehani H, Mohammadi-Sangcheshmeh A, Farifteh-Nobijari F, Salehi M Tags: Syst Biol Reprod Med Source Type: research

Good quality blastocyst from non-/mono-pronuclear zygote may be used for transfer during IVF.
Abstract Although healthy infants have developed from non- and mono-pronuclear zygotes, the transfer of embryos from non- and mono-pronuclear zygotes is not recommended because there are no proper selection criteria. In the present study, we discuss how to select non- and mono-pronuclear embryos with the highest developmental potential at 19-20 hours post-insemination. We found that the percentage of blastocysts with normal chromosome constitution in non-pronuclear zygotes was slightly higher than in mono-pronuclear zygotes. Non- and mono-pronuclear embryos that were at the 4-cell stage on D2 and/or at the 6- to 8...
Source: Systems Biology in Reproductive Medicine - February 22, 2016 Category: Reproduction Medicine Authors: Yin BL, Hao HY, Zhang YN, Wei D, Zhang CL Tags: Syst Biol Reprod Med Source Type: research

Effects of mechanical stresses on sperm function and fertilization rate in mice.
In this study, we investigated whether any of the observed changes in mouse sperm function tests secondary to mechanical stresses (centrifugation and pipetting) correlate with sperm fertilization ability. Chinese Kunming mice were used as sperm and oocyte donors. Sperm samples were allocated evenly into centrifugation, pipette, and control groups. Sperm plasma membrane integrity (PMI), mitochondrial membrane permeability (MMP), baseline and stimulated intracellular ROS, and sperm fertilization ability were measured by hypo-osmotic swelling, flow cytometry, and fertilization tests. Parallel studies were conducted and all te...
Source: Systems Biology in Reproductive Medicine - February 18, 2016 Category: Reproduction Medicine Authors: Shi X, Wang T, Qiu ZL, Li K, Li L, Chan CP, Chan SM, Li TC, Quan S Tags: Syst Biol Reprod Med Source Type: research

Clinical outcomes of frozen-thawed single blastocyst transfer in patients requiring whole embryo freezing.
Abstract We compared clinical outcomes amongst frozen-thawed cleavage-stage embryo, double and single blastocyst transfers in patients requiring whole embryo freezing. Data of infertile patients undergoing in-vitro fertilization and embryo transfer (IVF-ET) in our Reproductive Medicine Center from January 2010 to December 2012 were retrospectively analyzed. According to patients' wishes, patients were divided into cleavage-stage embryo transfer groups (group A, n = 456), double blastocyst transfer group (group B, n = 106), and single blastocyst transfer group (group C, n = 402). We found that the number of frozen ...
Source: Systems Biology in Reproductive Medicine - February 18, 2016 Category: Reproduction Medicine Authors: He QH, Wang L, Liang LL, Zhang HL, Zhang CL, Li HS, Cui SH Tags: Syst Biol Reprod Med Source Type: research

Low-dose ionizing irradiation triggers a 53BP1 response to DNA double strand breaks in mouse spermatogonial stem cells.
Abstract The present study aims to examine the effect of low-dose ionizing irradiation on DNA double strand breaks (DSB) in mouse spermatogonial stem cells (SSCs) and reveal the underlying pathways for the DNA repair for DSB in SSCs. Eighteen one-month-old mice were divided into 6 groups and sacrificed separately at 45 minutes, 2 hours, 24 hours, 48 hours, and 72 hours after 0.1Gy X-ray irradiation (mice without receiving ionizing irradiation served as control). After perfusion fixation, testes were removed, sectioned, and followed by staining of γH2AX, 53BP1, Caspase 3, and promyelocytic leukemia zinc-finge...
Source: Systems Biology in Reproductive Medicine - February 8, 2016 Category: Reproduction Medicine Authors: Le W, Qi L, Li J, Wu D, Xu J, Zhang J Tags: Syst Biol Reprod Med Source Type: research

Comparative systems genetics view of endometriosis and uterine leiomyoma: Two sides of the same coin?
Abstract Endometriosis (EM) and uterine leiomyoma (UL) are two most frequent benign tumors of monoclonal origin affecting about 30% of all women in their reproductive age. Modern molecular technologies have made a tremendous impact in understanding both disorders. Here is the first comparative analysis of molecular mechanisms underlying development of EM and UL as it looks from the platform of systems genetics. Similarities and differences of EM and UL at their incipient stages are enlightened with special emphasis on their gene networks, gene expression, and epigenetic regulation, of pathologic development. The a...
Source: Systems Biology in Reproductive Medicine - February 1, 2016 Category: Reproduction Medicine Authors: Baranov VS, Ivaschenko TE, Yarmolinskaya MI Tags: Syst Biol Reprod Med Source Type: research

Citrus limon extract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice.
Abstract The effect of oral administration of 50% ethanolic leaf extract of Citrus limon (500 and 1,000 mg/kg body weight/day) for 35 days on fertility and various male reproductive endpoints was evaluated in Parkes strain of mice. Testicular indices such as histology, 3β- and 17β-HSD enzymes activity, immunoblot expression of StAR and P450scc, and germ cell apoptosis by TUNEL and CASP- 3 expression were assessed. Motility, viability, and number of spermatozoa in the cauda epididymidis, level of serum testosterone, fertility indices, and toxicological parameters were also evaluated. Histologically...
Source: Systems Biology in Reproductive Medicine - January 22, 2016 Category: Reproduction Medicine Authors: Singh N, Singh SK Tags: Syst Biol Reprod Med Source Type: research

Therapeutic effects of quercetin against bisphenol A induced testicular damage in male Sprague Dawley rats.
Abstract The present study was designed to investigate protective effects of quercetin against bisphenol A (BPA) induced testicular toxicity in male Sprague Dawley rats. Twenty adult male rats were divided into four groups. The first group served as the control and was provided with normal saline. The second group of rats was treated with 50 mg/kg of BPA dissolved in alcoholic saline. The third group received oral gavage of 50 mg/kg quercetin while the fourth group was treated with quercetin (50 mg/kg) along with BPA (50 mg/kg). All of the treatments were carried out for 52 days. Testicular tissues and epididymis ...
Source: Systems Biology in Reproductive Medicine - January 19, 2016 Category: Reproduction Medicine Authors: Jahan S, Ain QU, Ullah H Tags: Syst Biol Reprod Med Source Type: research

A unique mosaic Turner syndrome patient with androgen receptor gene derived marker chromosome.
rakinci N Abstract Patients with Turner syndrome are generally characterized by having short stature with no secondary sexual characteristics. Some abnormalities, such as webbed neck, renal malformations (>50%) and cardiac defects (10%) are less common. The intelligence of these patients is considered normal. Non-mosaic monosomy X is observed in approximately 45% of postnatal patients with Turner syndrome and the rest of the patients have structural abnormalities or mosaicism involving 46,X,i(Xq), 45,X/46,XX, 45,X and other variants. The phenotype of 45,X/46,X,+mar individuals varies by the genetic continent an...
Source: Systems Biology in Reproductive Medicine - January 8, 2016 Category: Reproduction Medicine Authors: Kalkan R, Özdağ N, Bundak R, Çirakoğlu A, Serakinci N Tags: Syst Biol Reprod Med Source Type: research

Estrogen and progesterone differentially regulate carbonic anhydrase II, III, IX, XII, and XIII in ovariectomized rat uteri.
In conclusion, high expression of CAII, III, XII, and XIII under the influence of progesterone and estrogen plus progesterone could result in the reduction of uterine tissue and fluid pH; however, the significance of high levels of CAIX expression under the influence of estrogen remains unclear. PMID: 26709452 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 28, 2015 Category: Reproduction Medicine Authors: Karim K, Giribabu N, Muniandy S, Salleh N Tags: Syst Biol Reprod Med Source Type: research

WNT4-like protein is a cortical granule component in mouse oocytes and functions in regulating preimplantation embryogenesis.
Abstract Mammalian cortical granules (CG) are membrane-bound organelles located in the cortex of the unfertilized oocytes. Upon fertilization, CG undergo exocytosis to function in blocking polyspermy. While cortical granules are important in fertilization, their exact biochemical composition and reproductive function have not been fully defined. In the present study, a 66 kDa wingless-type MMTV integration site family, member 4 (WNT4)-like protein, with mouse CG origin was identified. Oocytes that were double labeled with lectin Lens culinaris agglutinin (LCA) and WNT4 antibody showed colocalization of the ...
Source: Systems Biology in Reproductive Medicine - December 23, 2015 Category: Reproduction Medicine Authors: Liu M, Yang HT Tags: Syst Biol Reprod Med Source Type: research

Complex preimplantation genetic diagnosis for beta-thalassaemia, sideroblastic anaemia, and human leukocyte antigen (HLA)-typing.
We describe a case where the couple who requested HLA-PGD, were both carriers of two serious haematological diseases, beta-thalassaemia and sideroblastic anaemia. Their daughter, affected with sideroblastic anaemia, was programmed to have HSCT. A multiplex-fluorescent-touchdown-PCR protocol was optimized for the simultaneous amplification of: the two HBB-gene mutated regions (c.118C > T, c.25-26delAA), four short tandem repeats (STRs) in chr11p15.5 linked to the HBB gene, the SLC25A38 gene mutation (c.726C > T), two STRs in chr3p22.1 linked to the SLC25A38 gene, plus eleven informative STR...
Source: Systems Biology in Reproductive Medicine - December 4, 2015 Category: Reproduction Medicine Authors: Kakourou G, Vrettou C, Kattamis A, Destouni A, Poulou M, Moutafi M, Kokkali G, Pantos K, Davies S, Kitsiou-Tzeli S, Kanavakis E, Traeger-Synodinos J Tags: Syst Biol Reprod Med Source Type: research

Impact of supplementary royal jelly on in vitro maturation of sheep oocytes: genes involved in apoptosis and embryonic development.
Abstract Optimizing culture conditions lead to the improvement of oocyte developmental competence and additives with anti-oxidative activity in culture media improved embryonic development. Royal jelly (RJ) is a product from the cephalic glands of nurse bees that has considerable health effects. The aim of this study was to investigate the effect of different concentrations of RJ on the maturation, cleavage, and blastocyst rates and gene expression in the oocyte and cumulus cells during in vitro maturation (IVM) of sheep oocyte. IVM of oocyte was performed in the presence of control (RJ0), 2.5 (RJ2.5), 5 (RJ5), 10...
Source: Systems Biology in Reproductive Medicine - December 4, 2015 Category: Reproduction Medicine Authors: Valiollahpoor Amiri M, Deldar H, Ansari Pirsaraei Z Tags: Syst Biol Reprod Med Source Type: research

Nitric oxide synthase in diabetic rat testicular tissue and the effects of pentoxifylline therapy.
l;nül E, Dündar M Abstract Diabetes is known to be associated with erectile dysfunction, retrograde ejaculation, level of testicular hormone, and a decrease in semen quality, respectively. In this project, we aimed to investigate at the molecular level, the effects of NOS on testes pathology in diabetes and examine the effects of pentoxifylline on healing. A total of 50 Wistar albino male rats were divided into five groups: Group I control; Group II only diabetes; Group III and IV diabetes + pentoxifylline; Group V only pentoxifylline. Group III rats received 50 mg/kg/day pentoxifyll...
Source: Systems Biology in Reproductive Medicine - November 13, 2015 Category: Reproduction Medicine Authors: Sönmez MF, Kılıç E, Karabulut D, Çilenk K, Deligönül E, Dündar M Tags: Syst Biol Reprod Med Source Type: research

The continuum of high ovarian response: a rational approach to the management of high responder patient subgroups.
Abstract Ovarian follicular responsiveness to controlled ovarian hyperstimulation (COH) with gonadotropins is extremely variable between individual patients, and even from cycle to cycle for the same patient. High responder patients are characterized by an exaggerated response to gonadotropin administration, accompanied by a higher risk for ovarian hyperstimulation syndrome (OHSS). In spite of its importance, the literature regarding high responders is characterized by heterogeneous classification methodologies. A clear separation should be drawn between risk factors for a high ovarian response and the actual resp...
Source: Systems Biology in Reproductive Medicine - October 30, 2015 Category: Reproduction Medicine Authors: Gat I, Shlush E, Quach K, Librach CL Tags: Syst Biol Reprod Med Source Type: research

Profiling of sperm proteins and association of sperm PDC-109 with bull fertility.
Abstract The composition of sperm proteins influences the fertilizing ability of sperm and hence the present study was conducted (i) to profile sperm proteins expression patterns in bulls of differing fertility index and (ii) to identify and relate the abundant sperm proteins with bull fertility. The semen samples were collected from Holstein-Friesian bulls (n = 12) varying in conception rate (CR) (high/low). The frozen semen straws (three ejaculates, from each bull) were used to study (a) sperm kinetic parameters, (b) plasmalemma integrity, (c) mitochondrial membrane potential, and (d) chromatin dis...
Source: Systems Biology in Reproductive Medicine - October 20, 2015 Category: Reproduction Medicine Authors: Somashekar L, Selvaraju S, Parthipan S, Ravindra JP Tags: Syst Biol Reprod Med Source Type: research

Identification of kinases, phosphatases, and phosphorylation sites in human and porcine spermatozoa.
Abstract Multiple inter-connected signaling pathways, involving kinases and phosphatases, form a framework that controls sperm motility, function, and fertilizing ability. Methods that give a broad view of the proteomic landscape may prove valuable in uncovering new crosstalk connections, as well as in discovering new proteins within this regulatory framework. A multi-immunoblotting strategy was utilized to evaluate this concept on human and porcine spermatozoa samples. In human and porcine spermatozoa, a diversity of kinases were identified including protein kinase A (PKA), protein kinase B (PKB), isoforms of pro...
Source: Systems Biology in Reproductive Medicine - October 15, 2015 Category: Reproduction Medicine Authors: Lackey BR, Gray SL Tags: Syst Biol Reprod Med Source Type: research

Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats.
Abstract Age related decline in reproductive performance in women is well documented and apoptosis has been considered as one of the reasons for the decline of primordial follicle reserve. Recently we observed a decline in the efficiency of DNA repair ability in aged rat primordial follicles as demonstrated by decreased mRNA levels of DNA repair genes BRCA1 and H2AX. In the present study, a two-dimensional electrophoresis (2DE) proteomic approach was employed to identify differentially expressed proteins in primordial follicles isolated from ovaries of immature (∼20 days) and aged (∼400-450 days) rats. Usi...
Source: Systems Biology in Reproductive Medicine - September 22, 2015 Category: Reproduction Medicine Authors: Govindaraj V, Rao AJ Tags: Syst Biol Reprod Med Source Type: research

Mammalian pre-implantation chromosomal instability: species comparison, evolutionary considerations, and pathological correlations.
Abstract Pre-implantation embryo development in mammals begins at fertilization with the migration and fusion of the maternal and paternal pro-nuclei, followed by the degradation of inherited factors involved in germ cell specification and the activation of embryonic genes required for subsequent cell divisions, compaction, and blastulation. The majority of studies on early embryogenesis have been conducted in the mouse or non-mammalian species, often requiring extrapolation of the findings to human development. Given both conserved similarities and species-specific differences, however, even comparison between cl...
Source: Systems Biology in Reproductive Medicine - September 14, 2015 Category: Reproduction Medicine Authors: Carbone L, Chavez SL Tags: Syst Biol Reprod Med Source Type: research

Nitric oxide and oxidative stress in placental explant cultures.
In conclusion, the levels of NO and lipid peroxidation follow a pattern similar to LDH and contrary to hCG. Future placental explant studies to evaluate oxidative stress and NO should consider the physiological changes inherent during the time of culture. PMID: 26366632 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 14, 2015 Category: Reproduction Medicine Authors: Goncalves JM, Casart YC, Camejo MI Tags: Syst Biol Reprod Med Source Type: research

Detection of DNA damage in cumulus cells using a chromatin dispersion assay.
We report the development of a chromatin dispersion test that allows for a fast evaluation of double strand DNA (ds-DNA) damage in CCs. The whole experiment was performed using CCs from 103 oocyte retrieval cycles evaluating the prototype D3-MAX ability (a chromatin dispersion based assay) to detect DNA breaks against in situ nick translation (ISNT) and a two tailed comet assay (TT-comet). Samples were collected from women younger than 35 years of age with a good response to stimulation. Pooled cumulus cells of MII oocytes were used. The chromatin dispersion assay results correlate with the double strand-DNA breaks values ...
Source: Systems Biology in Reproductive Medicine - August 25, 2015 Category: Reproduction Medicine Authors: Barcena P, López-Fernández C, García-Ochoa C, Obradors A, Vernaeve V, Gosálvez J, Vassena R Tags: Syst Biol Reprod Med Source Type: research

The first case of a small supernumerary marker chromosome derived from chromosome 10 in an adult woman with an apparently normal phenotype.
We report a new diagnosis of a mosaic sSMC (10) in a normal female who asked for genetic evaluation before undergoing controlled ovarian hyperstimulation, in vitro fertilization, and embryo transfer. Chromosome preparations from peripheral lymphocyte cultures were performed according to standard procedures. QFQ-banded chromosomes confirmed the presence of an sSMC: 47,XX,+mar[49]/46,XX[51]. FISH and array CGH analysis showed that the sSMC consisted of chromosome 10 with a gain of the 10p11.1p11.21 (2.5 Mb) chromosomal region. The presence of sSMC (10) was also confirmed in the patient's mother and sister. It did not ...
Source: Systems Biology in Reproductive Medicine - August 13, 2015 Category: Reproduction Medicine Authors: Santacroce R, Trunzo R, Leccese A, Pansini A, Gentile M, Margaglione M Tags: Syst Biol Reprod Med Source Type: research

The relationship between the changes in the level of progesterone and the outcome of in vitro fertilization-embryo transfer.
Abstract We observed the effects of changes in progesterone (P) during late follicular phases on the treatment outcome of in vitro fertilization-embryo transfer (IVF-ET) and intracytoplasmic sperm injection (ICSI) in patients with different ovarian responses. The data of 8,575 cycles of patients receiving gonadotropin-releasing hormone (GnRH) agonist using the long protocol were retrospectively analyzed. According to the number of oocytes retrieved, the cycles were divided into poor ovarian response group (oocyte retrieval
Source: Systems Biology in Reproductive Medicine - August 6, 2015 Category: Reproduction Medicine Authors: Dai W, Bu ZQ, Wang LL, Sun YP Tags: Syst Biol Reprod Med Source Type: research

Evidence for clock genes circadian rhythms in human full-term placenta.
lez C, Otero J, Díaz E Abstract Biological rhythms are driven by endogenous biological clocks; in mammals, the master clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus. This master pacemaker can synchronize other peripheral oscillators in several tissues such as some involved in endocrine or reproductive functions. The presence of an endogenous placental clock has received little attention. In fact, there are no studies in human full-term placentas. To test the existence of an endogenous pacemaker in this tissue we have studied the expression of circadian locomoter output cycles kapu...
Source: Systems Biology in Reproductive Medicine - August 6, 2015 Category: Reproduction Medicine Authors: Pérez S, Murias L, Fernández-Plaza C, Díaz I, González C, Otero J, Díaz E Tags: Syst Biol Reprod Med Source Type: research

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis.
This study represents to first use of sperm parameters together with NMR data as variables in the PCA analysis. Furthermore, this methodology allows the identification of the metabolites which play the most important role in identifying differences among human seminal plasma samples. PMID: 26236922 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 3, 2015 Category: Reproduction Medicine Authors: Bonechi C, Collodel G, Donati A, Martini S, Moretti E, Rossi C Tags: Syst Biol Reprod Med Source Type: research

Quantitative large scale gene expression profiling from human stem cell culture micro samples using multiplex pre-amplification.
Abstract Transcriptional profiling is a powerful tool to study biological mechanisms during stem cell differentiation and reprogramming. Genome-wide methods like microarrays or next generation sequencing are expensive, time consuming, and require special equipment and bioinformatics expertise. Quantitative RT-PCR remains one of today's most widely accepted and used methods for analyzing gene expression in biological samples. However, limitations in the amount of starting materials often hinder the quantity and quality of information that could be obtained from a given sample. Here, we present a fast 4-step workflo...
Source: Systems Biology in Reproductive Medicine - August 3, 2015 Category: Reproduction Medicine Authors: Kibschull M, Lye SJ, Okino ST, Sarras H Tags: Syst Biol Reprod Med Source Type: research

Editorial Retraction.
Authors: PMID: 26151735 [PubMed - in process] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 12, 2015 Category: Reproduction Medicine Tags: Syst Biol Reprod Med Source Type: research

Follicular fluid alterations in endometriosis: label-free proteomics by MS(E) as a functional tool for endometriosis.
This study provides a first step to the development of more sensitive diagnostic tests and treatment. PMID: 26114977 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 26, 2015 Category: Reproduction Medicine Authors: Regiani T, Cordeiro FB, da Costa LD, Salgueiro J, Cardozo K, Carvalho VM, Perkel KJ, Zylbersztejn DS, Cedenho AP, Lo Turco EG Tags: Syst Biol Reprod Med Source Type: research

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia.
This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) using spermatozoa from fresh and frozen testicular tissue from men with subgroups of non-obstructive azoospermia (NOA). A total of 110 cycles of TESE-ICSI were performed. Patients were classified into one of the following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage of embryo, and good quality embryo) and clinical (pregnancy, clinical pregnancy, implantation, and delivery) outcomes were ass...
Source: Systems Biology in Reproductive Medicine - June 26, 2015 Category: Reproduction Medicine Authors: Park YS, Lee SH, Lim CK, Cho JW, Yang KM, Seo JT Tags: Syst Biol Reprod Med Source Type: research

Six polymorphisms in genes involved in DNA double-strand break repair and chromosome synapsis: association with male infertility.
Abstract Four genes involved in DNA double-strand break repair and chromosome synapsis, i.e., testis expressed gene 11 (TEX11), testis expressed gene 15 (TEX15), mutL homolog 1 (MLH1), and homolog 3 (MLH3), play critical roles in genome integrity, meiotic recombination, and gametogenesis. We explored the possible association between single nucleotide polymorphisms (SNPs) in these genes and idiopathic male infertility involving azoospermia or oligozoospermia. A total of 614 fertile control and infertile men were recruited to this study in Sichuan, China. The latter group included 244 men with azoospermia and 72 men...
Source: Systems Biology in Reproductive Medicine - June 18, 2015 Category: Reproduction Medicine Authors: Zhang X, Ding M, Ding X, Li T, Chen H Tags: Syst Biol Reprod Med Source Type: research

The reproductive outcome of women with hypogonadotropic hypogonadism undergoing in vitro fertilization.
Abstract The aim of this study was to evaluate the reproductive outcome and assisted reproductive technology (ART) outcomes of patients with hypogonadotropic hypogonadism (HH) and to compare the results with male factor (MF) infertility patients. The reproductive outcome of 33 HH patients was evaluated retrospectively and compared with results of 47 patients with mild male factor infertility. For ovulation induction, human menopausal gonadotropin (hMG) was used in HH patients and recFSH was used in MF infertility patients. HH patients were divided into subgroups according to retrieved oocyte numbers and the groups...
Source: Systems Biology in Reproductive Medicine - June 4, 2015 Category: Reproduction Medicine Authors: Yilmaz S, Ozgu-Erdinc AS, Yumusak O, Kahyaoglu S, Seckin B, Yilmaz N Tags: Syst Biol Reprod Med Source Type: research

Effects of thymoquinone on testicular structure and sperm production in male obese rats.
This study investigated the effects of TQ on obesity and testicular structure of high-fat-diet (HFD) fed rats. Obese control (OC) and obese thymoquinone (OT) groups were fed a special diet containing 40% of total calories from fat. Non-obese control (NC) and non-thymoquinone (NT) groups were fed a standard diet for nine weeks. Then, intraperitoneal TQ injections were carried out to the OT and NT groups for six weeks and testes were removed. Catalase and myeloperoxidase activity were determined in rat testis tissue. Stereological, histopathological, and immunohistochemical changes were evaluated in the testes of the rats. I...
Source: Systems Biology in Reproductive Medicine - June 4, 2015 Category: Reproduction Medicine Authors: Tüfek NH, Altunkaynak ME, Altunkaynak BZ, Kaplan S Tags: Syst Biol Reprod Med Source Type: research

A rare Robertsonian translocation rob(14;22) carrier with azoospermia, meiotic defects, and testicular sperm aneuploidy.
This study shows the benefits of preimplantation genetic diagnosis in a case of a rare Robertsonian translocation carrier with azoospermia and a relatively low frequency of chromosomally unbalanced testicular spermatozoa. PMID: 26043179 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - June 4, 2015 Category: Reproduction Medicine Authors: Sobotka V, Vozdova M, Heracek J, Rubes J Tags: Syst Biol Reprod Med Source Type: research

Vascular endothelial growth factor (-460 C/T, +405 G/C, and +936 C/T) polymorphisms and endometriosis risk in Tunisian population.
This study includes 105 women with endometriosis and 150 women with no laparoscopic evidence of disease. Genotyping of the VEGF -460 C/T, +405 G/C, and +936 C/T polymorphisms were performed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The distribution of genotypes (P = 0.006) and allele (P = 0.0009) frequencies of the +936 C/T polymorphism was significantly different between patients and controls. Patients with stages III-IV endometriosis showed a higher VEGF + 936T allele frequency than controls (P = 0.0001). However, the distribu...
Source: Systems Biology in Reproductive Medicine - May 18, 2015 Category: Reproduction Medicine Authors: Henidi B, Kaabachi W, Naouali A, Kaabachi S, Zhioua A, Haj Sassi F, Hamzaoui K Tags: Syst Biol Reprod Med Source Type: research

Testing an egg yolk supplemented diet on boars to aid in sperm adaptation at 5°C.
Testing an egg yolk supplemented diet on boars to aid in sperm adaptation at 5°C. Syst Biol Reprod Med. 2015 May 12;:1-10 Authors: Casas I, Miller-Lux Y, Osborne B, Bonet S, Althouse GC Abstract In many species, extended semen can be stored at low temperatures to slow bacterial growth. However, boar semen performs poorly at temperatures below 15°C and this poses unique challenges, as it is not easy to maintain a constant 15-19°C during shipment. Some extenders have been formulated with egg yolk for storage at 5°C but the addition of egg yolk is not applicable in the majority of commerc...
Source: Systems Biology in Reproductive Medicine - May 12, 2015 Category: Reproduction Medicine Authors: Casas I, Miller-Lux Y, Osborne B, Bonet S, Althouse GC Tags: Syst Biol Reprod Med Source Type: research