Is the chronic use of Ferula harmonis to enhance mice erectile function effective and safe? A histopathological study.
This study aimed to investigate the impact of the chronic administration of F. hermonis root extract on the structure of the male mice reproductive organs and their fertility and to study the possible protective role of vitamin C. Sixty male albino mice were divided into 3 groups: the control, the experimental group that received F. hermonis root extract orally (6 mg/kg) for six weeks, and the treated group that received F. hermonis plus vitamin C for six weeks. Serum testosterone level and mice fertility were assessed. At the end of the experiment mice were sacrificed; testis, epididymis, and seminal vesicle were d...
Source: Systems Biology in Reproductive Medicine - May 28, 2014 Category: Reproduction Medicine Authors: Ayuob NN, Al-Harbi MS, Abdulhadi SS Tags: Syst Biol Reprod Med Source Type: research

Connexin 43 expression in Sprague-Dawley rat seminiferous epithelium after in utero exposure to flutamide.
This study explored the expression of connexin 43 (Cx43) in the testes of prepubertal Sprague-Dawley (SD) rats following in utero flutamide (Flu) exposure. Connexins constitute the major protein type in gap junctions. Connexin 43, the most prominent connexin family member expressed by testes, is localized at the base of seminiferous tubules in humans and rodents, and may be involved in fertility. Flutamide was injected subcutaneously into pregnant SD rats on gestational days 12-21 (25 mg/kg/day). Immunohistochemistry, Western blotting, and real-time PCR was used to investigate the distribution and the expression of ...
Source: Systems Biology in Reproductive Medicine - May 27, 2014 Category: Reproduction Medicine Authors: Cai C, Zhao D, Ma C, Zhang Y, Wu X, Wei G, He D Tags: Syst Biol Reprod Med Source Type: research

Recovery of ovary function impaired by chemotherapy using Chinese herbal medicine in a rat model.
In conclusion, Chinese herbal medicine showed a beneficial role in the recovery of ovary function in these rat models and has significant potential in the clinic. PMID: 24831605 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - May 15, 2014 Category: Reproduction Medicine Authors: Xia T, Fu Y, Gao H, Zhao Z, Zhao L, Han B Tags: Syst Biol Reprod Med Source Type: research

Mechanisms of oligozoospermia: an oxidative stress perspective.
Abstract Abstract Infertile patients presenting with poor semen concentration, motility, and morphology have significantly higher levels of reactive oxygen species (ROS). In this cross-sectional study, our goal was to: 1) determine how semen parameters such as concentration, motility, morphology, as well as ROS are altered in oligozoospermic men alone and those in combination with poor sperm concentration, motility, morphology, and compare these with three control groups; unproven donors, donors with proven fertility 2 years; 2) establish the cutoff, sensitivity, specificity for ROS, and see how it compared in the...
Source: Systems Biology in Reproductive Medicine - May 9, 2014 Category: Reproduction Medicine Authors: Agarwal A, Mulgund A, Sharma R, Sabanegh E Tags: Syst Biol Reprod Med Source Type: research

Successful pregnancy following assisted reproduction and transmyometrial embryo transfer in a patient with anatomical distortion of the cervical canal.
Abstract Abstract Presented is the case report of a patient noted to have gross distortion of the internal cervical canal during her attempt at embryo transfer following an in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI) procedure. Multiple attempts at cervical dilation were unsuccessful and the patient was ultimately treated by transmyometrial embryo transfer also known as the Towako method. She successfully achieved a singleton pregnancy and delivered at 41 weeks by primary cesarean section because of arrest of cervical dilation. Transmyometrial embryo transfer represents a viable option ...
Source: Systems Biology in Reproductive Medicine - May 5, 2014 Category: Reproduction Medicine Authors: Sullivan-Pyke CS, Kort DH, Sauer MV, Douglas NC Tags: Syst Biol Reprod Med Source Type: research

Effect of chocolate and Propolfenol on rabbit spermatogenesis and sperm quality following bacterial lipopolysaccharide treatment.
Abstract Abstract The aims of the study were to evaluate the effects of chocolate and propolis-enriched diets on rabbit spermatogenesis, sperm motility, and ultrastructure following bacterial lipopolysaccharide (LPS) treatment. Thirty-two New Zealand White rabbits were divided into four groups. The LPS-Propolfenol® group received propolis (500 mg/kg/day) in their diet for 15 days, while the LPS-chocolate group was fed 70% cacao chocolate (1 g/1 kg/day) for the same period. Following the diet treatments, rabbits in the LPS-Propolfenol® and LPS-chocolate groups, and an LPS group received...
Source: Systems Biology in Reproductive Medicine - April 30, 2014 Category: Reproduction Medicine Authors: Collodel G, Moretti E, Del Vecchio MT, Biagi M, Cardinali R, Mazzi L, Brecchia G, Maranesi M, Manca D, Castellini C Tags: Syst Biol Reprod Med Source Type: research

The role of mitochondrial aconitate (ACO2) in human sperm motility.
Abstract Abstract Deficiencies in tricarboxylic acid (TCA) cycle enzymes have been shown to cause a wide spectrum of human diseases, including malignancies and neurological and cardiac diseases. In mammalian spermatozoa mitochondria, the TCA cycle is known to be a crucial metabolic pathway that contributes to produce ATP. There is little known about the role and mechanism of mitochondrial aconitase (ACO2), which is an important regulatory enzyme of the TCA cycle, in asthenozoospermia. In the current study, immunofluorescence staining localized ACO2 to the human sperm mid-piece. By immunoblotting, we demonstrated t...
Source: Systems Biology in Reproductive Medicine - April 30, 2014 Category: Reproduction Medicine Authors: Tang M, Liu BJ, Wang SQ, Xu Y, Han P, Li PC, Wang ZJ, Song NH, Zhang W, Yin CJ Tags: Syst Biol Reprod Med Source Type: research

Protective effects of different antioxidants against cadmium induced oxidative damage in rat testis and prostate tissues.
Abstract Abstract The present study was performed to determine the effects of different antioxidants on testicular histopathology and oxidative damage induced by cadmium (Cd) in rat testis and prostate. Twenty five rats were equally divided into five groups (n = 5/group). The control group was injected subcutaneously with saline while the Cd alone treated group received a subcutaneous injection of 0.2 mg/kg CdCl2. Other groups were treated with sulphoraphane (25 µg/rat), vitamin E (75 mg/kg), and Ficus Religiosa plant extract (100 mg/kg) orally along with subcutaneou...
Source: Systems Biology in Reproductive Medicine - April 23, 2014 Category: Reproduction Medicine Authors: Jahan S, Zahra A, Irum U, Iftikhar N, Ullah H Tags: Syst Biol Reprod Med Source Type: research

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function.
Abstract Abstract Despite extensive research carried out for optimization and commercialization of sperm cryopreservation media, percentage of motility and viability remain low following cryopreservation. These adverse effects have been partially ascribed to reactive oxygen species (ROS) production during cryopreservation. Therefore, we proposed that addition of a cell permeable antioxidant like Tempol, with superoxide dismutase (SOD) mimetic action, may overcome these effects in an optimized commercially available cryo-protective medium. Therefore, semen samples were cryopreserved in the presence or absence of Te...
Source: Systems Biology in Reproductive Medicine - April 7, 2014 Category: Reproduction Medicine Authors: Bateni Z, Azadi L, Tavalaee M, Kiani-Esfahani A, Fazilati M, Nasr-Esfahani MH Tags: Syst Biol Reprod Med Source Type: research

Effects of occupational exposure - is there a link between exposure based on an occupational questionnaire and semen quality?
This study showed associations between self-reported occupational exposures and impaired semen parameters. The occupational exposure questionnaire may be useful in clinical practice for patients and physicians to identify the work factors associated with poorer semen quality. PMID: 24702586 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - April 7, 2014 Category: Reproduction Medicine Authors: Jurewicz J, Radwan M, Sobala W, Radwan P, Bochenek M, Hanke W Tags: Syst Biol Reprod Med Source Type: research

Effect of various commercial buffers on sperm viability and capacitation.
din L Abstract Abstract A wide variety of sperm preparation protocols are currently available for assisted conception. They include density gradient separation and washing methods. Both aim at isolating and capacitating as much motile sperm as possible for subsequent oocyte fertilization. The aim of this study was to examine the effects of four commercial sperm washing buffers on sperm viability and capacitation. Semen samples from 48 healthy donors (normal values of sperm count, motility, morphology, and volume) were analyzed. After separation (density gradient 40/80%), sperm were incubated in various buffers the...
Source: Systems Biology in Reproductive Medicine - March 27, 2014 Category: Reproduction Medicine Authors: Andrisani A, Donà G, Ambrosini G, Bonanni G, Bragadin M, Cosmi E, Clari G, Armanini D, Bordin L Tags: Syst Biol Reprod Med Source Type: research

Effect of oxamic analogues on functional mice sperm parameters.
uez-Páez L Abstract Abstract The present study evaluates the effect of oxamate derivatives (N-ethyl, N-propyl, N-butyl oxamates) on functional murine sperm parameters, towards a new male non-hormonal contraceptive. These derivatives are selective inhibitors of lactate dehydrogenase-C4 (LDH-C4). LDH-C4 is a sperm-specific enzyme that plays an important role in ATP production for maintaining progressive motility as well as to induce capacitation and hyperactivation. The results demonstrate that all oxamate derivatives selectively inhibited LDH-C4 in mouse sperm extracts. The IC50 values for hexokinase and gly...
Source: Systems Biology in Reproductive Medicine - March 21, 2014 Category: Reproduction Medicine Authors: Cordero-Martínez J, Aguirre-Alvarado C, Wong C, Rodríguez-Páez L Tags: Syst Biol Reprod Med Source Type: research

Liposome encapsulated soy lecithin and cholesterol can efficiently replace chicken egg yolk in human semen cryopreservation medium.
In conclusion, chemically defined cryopreservation medium with liposome encapsulated soy lecithin and cholesterol can effectively replace the chicken egg yolk from human semen cryopreservation medium without compromising post-thaw outcome. PMID: 24654557 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - March 21, 2014 Category: Reproduction Medicine Authors: Mutalik S, Salian SR, Avadhani K, Menon J, Joshi H, Hegde AR, Kumar P, Kalthur G, Adiga SK Tags: Syst Biol Reprod Med Source Type: research

Successful pregnancy after SrCl2 oocyte activation in couples with repeated low fertilization rates following calcium ionophore treatment.
This report describes a successful pregnancy and delivery following oocyte activation with strontium chloride (SrCl2) in couples with repeated complete fertilization failure or low fertilization rates even after calcium ionophore treatment. Eight infertile couples who showed complete fertilization failure or low fertilization rates after conventional intracytoplasmic sperm injection (ICSI) and calcium ionophore treatment. When the results of fertilization were not satisfactory in the cycles, the oocytes were artificially activated by SrCl2 for the next attempts. Oocyte activation with SrCl2 significantly increased the fert...
Source: Systems Biology in Reproductive Medicine - March 19, 2014 Category: Reproduction Medicine Authors: Kim JW, Kim SD, Yang SH, Yoon SH, Jung JH, Lim JH Tags: Syst Biol Reprod Med Source Type: research

Urinary thyroid hormone parameters test for evaluating the thyroid function during pregnancy.
This study collected the serum specimens of 30 healthy pregnant women at 9-12, 14-17, 23-26, and 37-40 weeks of gestation, respectively, simultaneously along with random urine specimens. This study compared the median levels of free triiodothyronine (FT3), free thyroxine (FT4), and thyrotropin (TSH) in serum and urine among four gestational stages. The differences were statistically significant (p 
Source: Systems Biology in Reproductive Medicine - March 13, 2014 Category: Reproduction Medicine Authors: Cai J, Zhao X, Lei T, Meng Q, Zhou H, Zhang M Tags: Syst Biol Reprod Med Source Type: research

Long-term study of vasectomy in Macaca radiata - histological and ultrasonographic analysis of testis and duct system.
This study was aimed to investigate the long-term effect of vasectomy using the bonnet monkey (Macaca radiata) as a primate animal model. Animals weighing around 6 to 8 kg were randomly chosen for bilateral, unilateral vasectomy and sham-control. The postoperative periods of six months and two years were considered as short and long-term, respectively. Sperm were collected and subjected to analysis before euthanasia. The testes and epididymides were excised from euthanized animals then embedded in paraffin. Normal histological changes were observed in sham-operated animals and short-term contralateral testes. In con...
Source: Systems Biology in Reproductive Medicine - March 4, 2014 Category: Reproduction Medicine Authors: Seppan P, Krishnaswamy K Tags: Syst Biol Reprod Med Source Type: research

Proteomic analysis of bovine blastocoel fluid and blastocyst cells.
sen C Abstract Abstract The understanding of the early mammalian development is a prerequisite for the advancement of in vitro fertilization and improvement of derivation and culturing of embryonic stem cells. While, whole genome transcriptomic analysis on bovine blastocysts has identified genes active in early development, little information is available about the protein complement of early embryos. Modern, sensitive proteomic technology (nano HPLC tandem mass spectrometry) allowed us to describe the proteome of the scarce blastocoel fluid and cell material of expanded bovine blastocysts isolated by micromanipul...
Source: Systems Biology in Reproductive Medicine - February 25, 2014 Category: Reproduction Medicine Authors: Jensen PL, Grøndahl ML, Beck HC, Petersen J, Stroebech L, Christensen ST, Yding Andersen C Tags: Syst Biol Reprod Med Source Type: research

The ESR1 gene in unexplained recurrent spontaneous abortion.
Abstract Abstract Recurrent spontaneous abortion (RSA) is a health problem that affects nearly 1% of fertile couples. However, the underlying etiology and mechanism(s) remain elusive. The aim of this study was to investigate estrogen receptor (ESR) 1 gene polymorphisms for risk association of unexplained recurrent spontaneous abortion (URSA) in the Chinese Han population. The entire coding region of the ESR1 gene was sequenced from 129 URSA patients and 183 healthy controls. There was a significant difference between the G allele and GG genotype distributions, of the ESR1 gene (XbaI) polymorphism, between the URSA...
Source: Systems Biology in Reproductive Medicine - February 25, 2014 Category: Reproduction Medicine Authors: Pan H, Suo P, Liu C, Wang J, Zhou S, Ma X, Wang B Tags: Syst Biol Reprod Med Source Type: research

Differential genes in adipocytes induced from polycystic and non-polycystic ovary syndrome-derived human embryonic stem cells.
Abstract Abstract We explored the molecular mechanisms of obesity and insulin resistance in patients with polycystic ovary syndrome (PCOS) using a human embryonic stem cell model (hESCs). Three PCOS-derived and one non-PCOS-derived hESC lines were induced into adipocytes, and then total RNA was extracted. The differentially expressed PCOS-derived and non-PCOS-derived adipocytes genes were identified using the Boao Biological human V 2.0 whole genome oligonucleotide microarray. Signals of interest were then validated by real-time PCR. A total of 153 differential genes were expressed of which 91 genes were up-regula...
Source: Systems Biology in Reproductive Medicine - February 12, 2014 Category: Reproduction Medicine Authors: Wang F, Liu WW, Chen XM, Kong HJ, Li J, Sun YP Tags: Syst Biol Reprod Med Source Type: research

Comparison of fertilization outcome between microdrop and open insemination methods in non-male factor IVF patients.
Abstract Abstract Both microdrop and open methods are commonly used for in vitro fertilization (IVF) protocols for embryo culture as well as oocyte insemination. However, few comparative studies evaluating the microdrop or open method of insemination on the fertilization outcome and subsequent embryo development have been performed. A randomized study was conducted to compare microdrop and open fertilization with respect to fertilization rate and embryo development among non-male factor patients undergoing in vitro fertilization and embryo transfer (IVF-ET). The results presented in this study demonstrate that the...
Source: Systems Biology in Reproductive Medicine - February 5, 2014 Category: Reproduction Medicine Authors: Li Y, Li T, Mai Q, Long L, Ou J Tags: Syst Biol Reprod Med Source Type: research

Alterations in the estrogen environment of the testis contribute to declining sperm production in aging rats.
This study was designed to test the hypothesis that the estrogen environment of the testis is altered during aging and that these changes are associated with declining sperm production. Sprague Dawley rats were examined at three, 15, 18, and 21 months of age to detail changes in sperm production and testicular concentration of testosterone and estradiol; five rats were used at three and 21 months and three rats were used at 15 and 18 months. Daily sperm production declined 49% from 15 to 21 months of age. Testicular concentrations of estradiol declined 53% from 15 to 21 months of age; testosterone concentrations were not s...
Source: Systems Biology in Reproductive Medicine - February 5, 2014 Category: Reproduction Medicine Authors: Clarke M, Pearl CA Tags: Syst Biol Reprod Med Source Type: research

SBiRM: Systems Biology in Reproductive Medicine - Year 7.
PMID: 24423097 [PubMed - in process] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - January 17, 2014 Category: Reproduction Medicine Authors: Krawetz S Tags: Syst Biol Reprod Med Source Type: research

Analysis of inter-examination differences in sperm nuclear vacuoles among male patients with infertility.
In conclusion, when multiple ejaculates were obtained, in the subset of male patients with infertility, the proportion of spermatozoa with LNVs could be different. The number of LNVs decreased following varicocele repair. PMID: 24423098 [PubMed - in process] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - January 17, 2014 Category: Reproduction Medicine Authors: Komiya A, Watanabe A, Kawauchi Y, Fuse H Tags: Syst Biol Reprod Med Source Type: research

Royal jelly protects from taxol-induced testicular damages via improvement of antioxidant status and up-regulation of E2f1.
This study was carried out to evaluate the protective effects of royal jelly (RJ) on taxol (TXL)-induced damage of the testis. Wistar rats were divided into control and test groups. The test group was divided into five subgroups; the first four groups along with TXL administration (7.5 mg/kg body weight (bw), weekly), received various doses of RJ (0, 50, 100, and 150 mg/kg bw). The last group received only RJ at 100 mg/kg. Royal jelly lowered the TXL-induced malondialdehyde and nitric oxide levels and enhanced the total thiol molecules in the testis. Remarkably RJ reduced the TXL-induced pathological i...
Source: Systems Biology in Reproductive Medicine - December 31, 2013 Category: Reproduction Medicine Authors: Delkhoshe-Kasmaie F, Malekinejad H, Khoramjouy M, Rezaei-Golmisheh A, Janbaze-Acyabar H Tags: Syst Biol Reprod Med Source Type: research

Erratum.
ERRATUM. Syst Biol Reprod Med. 2013 Dec 30; Authors: PMID: 24377703 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 30, 2013 Category: Reproduction Medicine Tags: Syst Biol Reprod Med Source Type: research

Increased IVF pregnancy rates after microarray preimplantation genetic diagnosis due to parental translocations.
Abstract Abstract We successfully performed preimplantation genetic diagnosis (PGD) and simultaneous preimplantation genetic screening (PGS) using single nucleotide polymorphism (SNP) microarrays for couples with balanced chromosome rearrangements in China. A total of 428 molecular karyotypes were diagnosed from 62 couples undergoing 68 in vitro fertilization (IVF) cycles. Of these, 48.1% of the embryos were chromosomally normal without translocation errors or aneuploidy. Of the 428 total embryos, 18.0% embryos were euploid, but were imbalanced due to the transmission of single translocation chromosome derivatives...
Source: Systems Biology in Reproductive Medicine - December 30, 2013 Category: Reproduction Medicine Authors: Li G, Jin H, Xin Z, Su Y, Brezina PR, Benner AT, Kearns WG, Sun Y Tags: Syst Biol Reprod Med Source Type: research

Concentration-dependent Sildenafil citrate (Viagra) effects on ROS production, energy status, and human sperm function.
Abstract Abstract Literature regarding the effects of sildenafil citrate on sperm function remains controversial. In the present study, we specifically wanted to determine if mitochondrial dysfunction, namely membrane potential, reactive oxygen species production, and changes in energy content, are involved in in vitro sildenafil-induced alterations of human sperm function. Sperm samples of healthy men were incubated in the presence of 0.03, 0.3, and 3 μM sildenafil citrate in a phosphate buffered saline (PBS)-based medium for 2, 3, 12, and 24 hours. Sperm motility and viability were evaluated and mitoch...
Source: Systems Biology in Reproductive Medicine - December 18, 2013 Category: Reproduction Medicine Authors: Sousa MI, Amaral S, Tavares RS, Paiva C, Ramalho-Santos J Tags: Syst Biol Reprod Med Source Type: research

Motility, viability, and calcium in the sperm cells.
Abstract Abstract Sperm cells are complicated in vitro models. Their viability is limited, and physiology is complex. The study of their properties is of great application in the animal production as viable and functional gametes are essential. It has been shown that the decrease of sperm cell viability parallels an increase of the reactive oxygen species (ROS). Reactive oxygen species is secondary to normal metabolic processes of the cell-like flagellar movement. There is evidence of strategies that reduce ROS levels by using exogenous or endogenous antioxidants with the intention that seminal plasma protects the...
Source: Systems Biology in Reproductive Medicine - December 13, 2013 Category: Reproduction Medicine Authors: Parodi J Tags: Syst Biol Reprod Med Source Type: research

Role of oxygen in the regulation of Leydig tumor derived MA-10 cell steroid production: the effect of cobalt chloride.
This study suggests that hypoxia could be as important as gonadotropins in regulating Leydig cell steroidogenesis. PMID: 24328340 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 12, 2013 Category: Reproduction Medicine Authors: Kumar A, Rani L, Dhole B Tags: Syst Biol Reprod Med Source Type: research

Erratum.
Authors: PMID: 24304326 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - December 5, 2013 Category: Reproduction Medicine Tags: Syst Biol Reprod Med Source Type: research

Endometrial receptivity markers in infertile women stimulated with letrozole compared with clomiphene citrate and natural cycles.
Abstract Abstract Though various advantages associated with the use of letrozole as an ovulation inducing drug are documented, there is inadequate information regarding its effect on endometrial receptivity. Expression of endometrial receptivity markers including αvβ3 integrin, L-selectin, LIF, and pinopods during the implantation window in infertile women stimulated with letrozole or clomiphene citrate (CC), and spontaneous cycles is investigated. A total of 36 women were included in the study out of which 16 women were diagnosed with primary unexplained infertility and the other 20 women with severe o...
Source: Systems Biology in Reproductive Medicine - December 5, 2013 Category: Reproduction Medicine Authors: Ganesh A, Chauhan N, Das S, Chakravarty B, Chaudhury K Tags: Syst Biol Reprod Med Source Type: research

Oxidative status shifts in uterine cervical incompetence patients.
Abstract Abstract Uterine cervical incompetence (UCI) is a pregnancy complication affecting about 10% of the pregnancies in the western world. Studying the etiology of the UCI requires a specific approach adequate for this highly heterogenous syndrome. Oxidative status disorders are associated with various pathologies, including pregnancy complications. As such, general oxidative status profiling is a promising methodology to treat UCI. We aimed at assaying the closely interrelated oxidative status markers in the uterine cervical incompetence patients by means of the systems biology-oriented approach. Chemilumines...
Source: Systems Biology in Reproductive Medicine - December 5, 2013 Category: Reproduction Medicine Authors: Zolotukhin P, Aleksandrova A, Goncharova A, Shestopalov A, Rymashevskiy A, Shkurat T Tags: Syst Biol Reprod Med Source Type: research

Association between TP53 gene Arg72Pro polymorphism and idiopathic infertility in southeast Chinese Han males.
This study indicated that the TP53 gene Arg72Pro polymorphism of the spermatogenic pathway may be associated with idiopathic infertility in southeast Chinese Han males. PMID: 24266512 [PubMed - in process] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - November 27, 2013 Category: Reproduction Medicine Authors: Jin Q, Wang B, Wang J, Liu T, Yu X, Jia C, Fang X, Peng Y, Ma X Tags: Syst Biol Reprod Med Source Type: research

(1)H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential.
The objective of this study was to employ NMR based metabolic profiling analysis of spent embryo culture media to identify novel biomarkers of embryo viability and provide insight into the metabolism of a viable embryo. A total of 37 patients undergoing IVF/ICSI treatment were recruited and 58 media samples were collected from embryos that were transferred back to the uterus. (1)H NMR spectra were acquired and analyzed resulting in the quantification of 12 metabolites in the media samples. Analysis of metabolite ratios revealed significant differences between those patients with positive (n = 27) and negative...
Source: Systems Biology in Reproductive Medicine - November 21, 2013 Category: Reproduction Medicine Authors: Wallace M, Cottell E, Cullinane J, McAuliffe FM, Wingfield M, Brennan L Tags: Syst Biol Reprod Med Source Type: research

Lycopene, a powerful antioxidant, significantly reduces the development of the adhesion phenotype.
In this study, we sought to determine whether lycopene, a potent antioxidant, reduces markers characteristic of the adhesion phenotype. Primary fibroblast cultures from normal peritoneum and adhesion tissues were utilized to determine mRNA levels of adhesion phenotype markers type I collagen, transforming growth factor-beta 1 (TGF-β1), and vascular endothelial growth factor (VEGF) in response to lycopene (24 hours, 10 μM) treatment. There was a 2 (p 
Source: Systems Biology in Reproductive Medicine - November 12, 2013 Category: Reproduction Medicine Authors: Fletcher NM, Awonuga AO, Saed MG, Abu-Soud HM, Diamond MP, Saed AG Tags: Syst Biol Reprod Med Source Type: research

Comparative analysis of antioxidants against cadmium induced reproductive toxicity in adult male rats.
Abstract Abstract The present study was conducted to compare and evaluate the potential benefits of three different antioxidants in reversing cadmium (Cd)-induced reproductive toxicity in adult male rats. Rats (n = 5) weighing 180 ± 20 gm were divided into five groups (control, Cd, Cd + sulforaphane, Cd + vitamin E, and Cd + plant extract). Treated groups received CdCl2 (0.2 mg/kg), sulforaphane (25 µg/rat), vitamin E (75 mg/kg), and plant extract (100 mg/kg) for 15 days. Blood samples and testicul...
Source: Systems Biology in Reproductive Medicine - October 24, 2013 Category: Reproduction Medicine Authors: Jahan S, Khan M, Ahmed S, Ullah H Tags: Syst Biol Reprod Med Source Type: research

Copper: a biphasic regulator of caprine sperm forward progression.
Abstract Abstract Copper is essential for spermatogenesis and its presence has been demonstrated in male and female reproductive fluids in several mammalian species. However, little is known about the physiological significance of this trace element in the regulation of forward progression of mammalian sperm cells which is essential for sperm fertility potential in vivo. The purpose of this investigation was to determine the physiological role of the bivalent copper ion (Cu(2+)) on mammalian sperm forward motility using a chemically-defined medium and caprine cauda epididymal sperm model. Sperm forward motility wa...
Source: Systems Biology in Reproductive Medicine - October 24, 2013 Category: Reproduction Medicine Authors: Roy D, Dey S, Majumder GC, Bhattacharyya D Tags: Syst Biol Reprod Med Source Type: research

Ovarian cancer evolution through stochastic genome alterations: defining the genomic role in ovarian cancer.
Abstract Abstract Ovarian cancer is the fifth leading cause of death among women worldwide. Characterized by complex etiology and multi-level heterogeneity, its origins are not well understood. Intense research efforts over the last decade have furthered our knowledge by identifying multiple risk factors that are associated with the disease. However, it is still unclear how genetic heterogeneity contributes to tumor formation, and more specifically, how genome-level heterogeneity acts as the key driving force of cancer evolution. Most current genomic approaches are based on 'average molecular profiling.' While eff...
Source: Systems Biology in Reproductive Medicine - October 22, 2013 Category: Reproduction Medicine Authors: Abdallah BY, Horne SD, Kurkinen M, Stevens JB, Liu G, Ye CJ, Barbat J, Bremer SW, Heng HH Tags: Syst Biol Reprod Med Source Type: research

Lifestyle and semen quality: role of modifiable risk factors.
Abstract Abstract The relationship between exposure to lifestyle factors and adverse effects on human reproductive health is debated in the scientific literature and these controversies have increased public and regulatory attention. The aim of the study was to examine the association between modifiable lifestyle factors and main semen parameters, sperm morphology, and sperm chromatin structure. The study population consisted of 344 men who were attending an infertility clinic for diagnostic purposes with normal semen concentration of 20-300 M/ml or with slight oligozoospermia (semen total concentration of ...
Source: Systems Biology in Reproductive Medicine - September 30, 2013 Category: Reproduction Medicine Authors: Jurewicz J, Radwan M, Sobala W, Ligocka D, Radwan P, Bochenek M, Hanke W Tags: Syst Biol Reprod Med Source Type: research

Somatic and reproductive development in pre-pubertal mice treated with cyclophosphamide and subsequent estrogen replacement.
Abstract We tested the hypothesis that chemotherapy would prevent the expected pubertal development of uterus, ovaries, and long bones, and that estrogen replacement subsequent to treatment with chemotherapy would restore uterine and bone development to expected sizes. Pre-pubertal female C57BL/6J mice (n = 78) were assigned to receive placebo (controls), 200 mg/kg (group A), or 120 mg/kg (group B) of cyclophosphamide (CTX) on postnatal day 18. Mice were subsequently randomized to receive estradiol placebo or long-release estradiol pellet insertion on day 22 (early estradiol dose), day ...
Source: Systems Biology in Reproductive Medicine - September 25, 2013 Category: Reproduction Medicine Authors: Uhlmann RA, Ogwo C, Williams RW, Osborne SE, Williams LJ, Detti L Tags: Syst Biol Reprod Med Source Type: research

Association of interleukin-1beta C + 3953T gene polymorphism with human male infertility.
Association of interleukin-1beta C + 3953T gene polymorphism with human male infertility. Syst Biol Reprod Med. 2013 Sep 25; Authors: Jaiswal D, Trivedi S, Agrawal NK, Singh R, Singh K Abstract Cytokines are involved in the regulation of spermatogenesis likely mediating the crosstalk among Sertoli and germ cells to facilitate germ cell movement across the seminiferous epithelium during cellular events such as germ cell differentiation. Members of the Interleukin-1 (IL-1) family are pleiotropic cytokines that are involved in inflammation, immunoregulation, and other homeostatic functions. Inte...
Source: Systems Biology in Reproductive Medicine - September 25, 2013 Category: Reproduction Medicine Authors: Jaiswal D, Trivedi S, Agrawal NK, Singh R, Singh K Tags: Syst Biol Reprod Med Source Type: research

IFN-gamma alters the human sperm membrane permeability to Ca(2)(+)
In conclusion, in vitro, IFN-γ modifies Ca(2+) sperm membrane permeability, probably via lipid peroxidation. IFN-γ in high concentration, as observed in inflammation/infection, can affect [Ca(2+)]cyto regulation and alter sperm fertilizing capacity. PMID: 24067141 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - September 25, 2013 Category: Reproduction Medicine Authors: Carrasquel G, Camejo MI, Michelangeli F, Ruiz MC Tags: Syst Biol Reprod Med Source Type: research

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?
Abstract The aim of this study was to determine if there are any associations between impaired chromatin packaging and the origin of reactive oxygen species (ROS) production. Cytosolic ROS, mitochondrial ROS, DNA protamination, and apoptosis were evaluated with dichlorofluoresceindiacetate (DCFH-DA), dihydrorhodamine 123 (DHR123), chromomycin A3 (CMA3), and YO-Pro-1 (Y1)/propidium iodide (PI), respectively, by flow cytometry (FCM) in 40 infertile individuals. Percentages DCF+ and R123+ sperm were positively associated with percentage CMA3+ sperm and negatively associated with percentage apoptotic sperm. No correla...
Source: Systems Biology in Reproductive Medicine - August 22, 2013 Category: Reproduction Medicine Authors: Kiani-Esfahani A, Bahrami S, Tavalaee M, Deemeh MR, Mahjour AA, Nasr-Esfahani MH Tags: Syst Biol Reprod Med Source Type: research

Protective effect of Cymbopogon citratus on hydrogen peroxide-induced oxidative stress in the reproductive system of male rats.
This study was conducted to evaluate the protective effect of C. citratus aqueous extract against hydrogen peroxide (H2O2)-induced oxidative stress and injury in the reproductive system of male rats. The twenty-five rats used in this study were divided into five groups, comprised of five rats each. The control group received standard food and drink. The H2O2 group received standard food and water with 0.5% H2O2. The rats in the H2O2 + C. citratus group and H2O2 + vitamin E group received standard food, H2O2, and C. citratus [100 mg·kg(-1) body weight (bw)], or vitamin E as an antio...
Source: Systems Biology in Reproductive Medicine - August 19, 2013 Category: Reproduction Medicine Authors: Rahim SM, Taha EM, Mubark ZM, Aziz SS, Simon KD, Mazlan AG Tags: Syst Biol Reprod Med Source Type: research

Dual effects of molybdenum on mouse oocyte quality and ovarian oxidative stress.
In conclusion, Mo affects oocyte quality possibly through regulating ovarian oxidative stress in a dose-dependent manner. It appears that Mo may improve ovarian function at a suitable concentration, which might be a candidate for the treatment of female infertility. PMID: 23947923 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 16, 2013 Category: Reproduction Medicine Authors: Zhang YL, Liu FJ, Chen XL, Zhang ZQ, Shu RZ, Yu XL, Zhai XW, Jin LJ, Ma XG, Qi Q, Liu ZJ Tags: Syst Biol Reprod Med Source Type: research

Biochemical characterization of stallion prostasomes and comparison to their human counterparts.
We present some new proteins of horse prostasomes not found in their human counterparts. Further studies are warranted to reveal the function of these proteins. PMID: 23909385 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - August 2, 2013 Category: Reproduction Medicine Authors: Ronquist GK, Ek B, Ronquist G, Morrell J, Carlsson L, Larsson A Tags: Syst Biol Reprod Med Source Type: research

The relationship between seminal leukocytes, oxidative status in the ejaculate, and apoptotic markers in human spermatozoa.
Abstract The aim of this study was to investigate the relationship between seminal leukocytes, reactive oxygen species (ROS) production in the ejaculate, and markers of apoptosis in human spermatozoa. Semen samples were collected from 60 patients attending fertility clinics at the Reproductive Biology Unit at Tygerberg Academic Hospital and Vincent Pallotti Hospital, Cape Town, South Africa. The concentration of seminal leukocytes was determined and was correlated with ROS production in the ejaculate, the percentage of superoxide (·O2(-))- and hydrogen peroxide (H2O2)-positive spermatozoa, glutathione activ...
Source: Systems Biology in Reproductive Medicine - July 30, 2013 Category: Reproduction Medicine Authors: Mupfiga C, Fisher D, Kruger T, Henkel R Tags: Syst Biol Reprod Med Source Type: research

Antidiabetic effect of α-mangostin and its protective role in sexual dysfunction of streptozotocin induced diabetic male rats.
Antidiabetic effect of α-mangostin and its protective role in sexual dysfunction of streptozotocin induced diabetic male rats. Syst Biol Reprod Med. 2013 Jul 25; Authors: Nelli GB, K AS, Kilari EK Abstract Sexual dysfunction is one of the diabetic complications in males. The present study aimed to evaluate the antidiabetic effect of α-mangostin and its protective role in sexual dysfunction of streptozotocin (STZ) induced diabetic male rats. Male Wistar rats were divided as control, diabetic control, diabetic rats administered with 25, 50 mg/kg body weight (bw) of α-mangostin and 1&...
Source: Systems Biology in Reproductive Medicine - July 25, 2013 Category: Reproduction Medicine Authors: Nelli GB, K AS, Kilari EK Tags: Syst Biol Reprod Med Source Type: research

Evaluation of the effectiveness of semen storage and sperm purification methods for spermatozoa transcript profiling.
This study supports the view that PS purification better maintains the integrity of spermatozoal RNAs. PMID: 23869956 [PubMed - as supplied by publisher] (Source: Systems Biology in Reproductive Medicine)
Source: Systems Biology in Reproductive Medicine - July 19, 2013 Category: Reproduction Medicine Authors: Mao S, Goodrich RJ, Hauser R, Schrader SM, Chen Z, Krawetz SA Tags: Syst Biol Reprod Med Source Type: research

Expression of stem cell markers: OCT4, KIT, ITGA6, and ITGB1 in the male germinal epithelium.
usa M Abstract Efforts have been made for the isolation and characterization of human stem spermatogonia (SG) which would be of major interest for fertility preservation in oncologic patients. We evaluated the expression of mammalian SG stem cell markers, KIT, OCT4, integrin alpha 6 (ITGA6), and integrin beta 1 (ITGB1) as possible indicators for the isolation of those cells in humans. Two different types of SG were individually isolated by micromanipulation from testicular biopsies of men with conserved spermatogenesis. Expression of mRNA showed the absence of KIT and ITGB1 markers in SG. By immunocytochemistry (I...
Source: Systems Biology in Reproductive Medicine - June 13, 2013 Category: Reproduction Medicine Authors: Sá R, Miranda C, Carvalho F, Barros A, Sousa M Tags: Syst Biol Reprod Med Source Type: research