Host defence related responses in bovine milk during an experimentally induced Streptococcus uberis infection
Conclusions:
These data provide a foundation for understanding the role of milk in host-microbe interaction. Furthermore they provide candidate biomarkers for mastitis diagnosis, and will inform efforts to develop dairy products with improved health-promoting properties. (Source: Proteome Science)
Source: Proteome Science - April 11, 2014 Category: Bioinformatics Authors: Grant SmolenskiMarita BroadhurstKerst StelwagenBrendan HaighThomas Wheeler Source Type: research
An improved peptide-spectral matching algorithm through distributed search over multiple cores and multiple CPUs
Conclusions:
The improved RT-PSM algorithms can achieve the processing speed requirement without sacrificing the level of inference accuracy. With some configuration adjustments, the proposed algorithm can support many peptide identification programs, such as X!Tandem, CUDA version RT-PSM, etc. (Source: Proteome Science)
Source: Proteome Science - April 11, 2014 Category: Bioinformatics Authors: Jian SunBolin ChenFang-Xiang Wu Source Type: research
Maize IgE binding proteins: each plant a different profile?
Conclusions:
These results lead us to conclude that, within each species, plant allergenic potential varies with genotype. Moreover, considering the stress-related IgE binding proteins identified, we hypothesise that the environment, particularly stress conditions, may alter IgE binding protein profiles of plant components. (Source: Proteome Science)
Source: Proteome Science - March 20, 2014 Category: Bioinformatics Authors: Cátia FonsecaSébastien PlanchonCarla PinheiroJenny RenautCândido RicardoM OliveiraRita Batista Source Type: research
SILAC labeling coupled to shotgun proteomics analysis of membrane proteins of liver stem/hepatocyte allows to candidate the inhibition of TGF-beta pathway as causal to differentiation
Conclusions:
Taken together, this study increases the understanding of the underlying mechanisms modulating the complex biological processes of hepatic stem cell proliferation and differentiation. (Source: Proteome Science)
Source: Proteome Science - March 15, 2014 Category: Bioinformatics Authors: Claudia MontaldoCarmine ManconeAlice ConigliaroAngela CozzolinoValeria de NonnoMarco Tripodi Source Type: research
Metabolic labeling with stable isotope nitrogen (15N) to follow amino acid and protein turnover of three plastid proteins in Chlamydomonas reinhardtii
Conclusion:
We describe a more rapid and non-radioactive method to measure free amino acid and protein turnover. Our approach is applicable for determination of protein turnover for various proteins, which will lead to a better understanding of the relationship between protein lifetime and functionality. (Source: Proteome Science)
Source: Proteome Science - March 3, 2014 Category: Bioinformatics Authors: Marie-Laure SauerBing XuFedora Sutton Source Type: research
Proteogenomics of selective susceptibility to endotoxin using circulating acute phase biomarkers and bioassay development in sheep: a review
Scientists have injected endotoxin into animals to investigate and understand various pathologies and novel therapies for several decades. Recent observations have shown that there is selective susceptibility to Escherichia coli lipopolysaccharide (LPS) endotoxin in sheep, despite having similar breed characteristics. The reason behind this difference is unknown, and has prompted studies aiming to explain the variation by proteogenomic characterisation of circulating acute phase biomarkers. It is hypothesised that genetic trait, biochemical, immunological and inflammation marker patterns contribute in defining and predicti...
Source: Proteome Science - March 1, 2014 Category: Bioinformatics Authors: Saul ChemongesJohn-Paul TungJohn Fraser Source Type: research
Proteomic analysis on effectors involved in BMP-2-induced osteogenic differentiation of beagle bone marrow mesenchymal stem cells
Conclusions:
Among the 20 rhBMP-2-regulated factors, there is empirical evidence supporting the involvement of LASP1 and ferritin in osteogenic differentiation. LASP1 plays an important role in the regulation of the activity of the cytoskeleton, and ferritin is an important molecule in cellular iron homeostasis. Further studies focused on these 20 proteins will help elucidate the molecular mechanism(s) through which rhBMP-2 induces osteogenic differentiation of BMSCs. (Source: Proteome Science)
Source: Proteome Science - March 1, 2014 Category: Bioinformatics Authors: Ji-jie HuYa-wei LiuMin-yi HeDan JinHui ZhaoBin Yu Source Type: research
Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine
Conclusion:
Brain cortex of rats exposed to increasing doses of morphine is far from being adapted. Significant up-regulation of proteins functionally related to oxidative stress and apoptosis suggests a major change of energy metabolism resulting in the state of severe brain cell "discomfort" or even death. (Source: Proteome Science)
Source: Proteome Science - February 14, 2014 Category: Bioinformatics Authors: Hana UjcikovaAdam EckhardtDmytro KaganLenka RoubalovaPetr Svoboda Source Type: research
Proteomic sample preparation for blast wound characterization
Conclusion:
High abundant protein removal was necessary for both wound effluent and serum. This is the first study to show a successful method for high abundant protein depletion from wound effluent which is compatible with downstream 2-D DIGE analysis. This development allows for improved biomarker discovery in wound effluent and serum samples. (Source: Proteome Science)
Source: Proteome Science - February 14, 2014 Category: Bioinformatics Authors: Brett ChromyAngela EldridgeJonathan ForsbergTrevor BrownBenjamin KirkupEric ElsterPaul Luciw Source Type: research
Glucose induces rapid changes in the secretome of Saccharomyces cerevisiae
Conclusions:
Our data indicate that transferring cells from low to high glucose media induces a rapid decline in levels of a large number of extracellular proteins and the disappearance of small vesicles from the extracellular fraction. Therefore, we conclude that the secretome undergoes dynamic changes during transition from glucose-deficient to glucose-rich media. Most of these extracellular proteins do not contain typical ER signal sequences, suggesting that they are secreted via the non-classical pathway. (Source: Proteome Science)
Source: Proteome Science - February 12, 2014 Category: Bioinformatics Authors: Bennett GiardinaBruce StanleyHui-Ling Chiang Source Type: research
Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry
Conclusions:
This is the first study to report the accumulation of gliadins containing odd numbers of cysteine residues in the SDS-extractable glutenin polymer fraction, supporting the hypothesis that these gliadins serve as chain terminators of the polymer chains. These data make it possible to formulate hypotheses about how protein composition influences polymer size and structure and provide a foundation for future experiments aimed at determining how environment affects glutenin polymer distribution. In addition, the analysis revealed additional layers of complexity to the wheat flour proteome that should be considered...
Source: Proteome Science - February 11, 2014 Category: Bioinformatics Authors: William VenselCharlene TanakaSusan Altenbach Source Type: research
Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)
Conclusions:
In a conclusion, different proteins in response to Marssonina coronaria were identified by proteomic analysis. Among of these proteins, there are some PR proteins, for example class III endo-chitinase, beta-1,3-glucanase and thaumatine-like protein, and some antioxidant related proteins including aldo/keto reductase AKR, ascorbate peroxidase and phi class glutathione S-transferase protein that were associated with disease resistance. The transcription levels of class III endo-chitinase (L13) and beta-1, 3-glucanase (L17) have a good relation with the abundance of the encoded protein's accumulation, however, th...
Source: Proteome Science - February 7, 2014 Category: Bioinformatics Authors: Miaomiao LiJianhua XuZonghao QiuJuan ZhangFengwang MaJunke Zhang Source Type: research
Fast fixing and comprehensive identification to help improve real-time ligands discovery based on formaldehyde crosslinking, immunoprecipitation and SDS-PAGE separation
Conclusions:
Fast cross-linking was achieved. The 4 F strategy can be used to identify real-time in situ interactions without prior intervention and to comprehensively identify ligands of particular target proteins with fewer false positives. (Source: Proteome Science)
Source: Proteome Science - February 1, 2014 Category: Bioinformatics Authors: Lisi ZhuMenglin LiLilong WeiXuejiao LiuJianrui YinYouhe Gao Source Type: research
Plasma protein profiling of Mild Cognitive Impairment and Alzheimer¿s disease using iTRAQ quantitative proteomics
Conclusion:
These findings demonstrate that expression level changes in multiple proteins are observed in MCI and AD plasma. Some of these, such as afamin and IGHM, may be candidate biomarkers for AD and the predementia condition of MCI. (Source: Proteome Science)
Source: Proteome Science - January 17, 2014 Category: Bioinformatics Authors: Fei SongAnne PoljakNicole KochanMark RafteryHenry BrodatyGeorge SmythePerminder Sachdev Source Type: research
Comparative proteomics analysis of oral cancer cell lines: identification of cancer associated proteins
Conclusions:
The outcome of this analysis demonstrated that a comparative study of whole cell proteome of cancer versus normal cell lines can be used to identify cancer associated proteins. (Source: Proteome Science)
Source: Proteome Science - January 15, 2014 Category: Bioinformatics Authors: Saiful KarsaniNor SaihenRosnah ZainSok-Ching CheongMariati Abdul Rahman Source Type: research
