Colorectal cancer derived organotypic spheroids maintain essential tissue characteristics but adapt their metabolism in culture
Conclusions:
Our analysis of organotypic CRC tumor spheroids has identified biological processes associated with a mixture of cell types and states, including protein markers for mesenchymal and stem-like cells. This 3D tumor model in which tumor heterogeneity is preserved may represent an advantageous model system to investigate novel therapeutic approaches. (Source: Proteome Science)
Source: Proteome Science - July 11, 2014 Category: Bioinformatics Authors: Uros RajcevicJaco KnolSander PiersmaSébastien BougnaudFred FackEirik SundlisaeterKarl SøndenaaReidar MyklebustThang PhamSimone NiclouConnie Jiménez Source Type: research
Comparative proteomic analysis of the membrane proteins of two Haemophilus parasuis strains to identify proteins that may help in habitat adaptation and pathogenesis
Conclusions:
The outcome demonstrated the presence of some proteins which raise the speculation for their importance in helping in habitat adaption or pathogenesis within the host. (Source: Proteome Science)
Source: Proteome Science - July 7, 2014 Category: Bioinformatics Authors: Luhua ZhangYiping WenYing LiXingliang WeiXuefeng YanXintian WenRui WuXiaobo HuangYong HuangQigui YanMafeng LiuSanjie Cao Source Type: research
Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
Conclusions:
The differential expression of three proteins (enolase, esterase and ADH3) induced by PEG6 treatment could play an important role in maturation, and these proteins could be characterized as candidate biomarkers of somatic embryogenesis in papaya. (Source: Proteome Science)
Source: Proteome Science - June 26, 2014 Category: Bioinformatics Authors: Ellen de Moura ValeAngelo HeringerTatiana BarrosoAndré da Silva FerreiraMonique da CostaJonas PeralesClaudete Santa-CatarinaVanildo Silveira Source Type: research
Comparative secretome analysis of Streptomyces scabiei during growth in the presence or absence of potato suberin
Conclusion:
This study demonstrated that the presence of suberin in S. scabiei growth medium induced the production of a wide variety of glycosyl hydrolases. Furthermore, this study has allowed the identification of extracellular enzymes that could be involved in the degradation of suberin, including enzymes of the lipid metabolism and feruloyl esterases. (Source: Proteome Science)
Source: Proteome Science - June 25, 2014 Category: Bioinformatics Authors: Doaa KomeilRebeca Padilla-ReynaudSylvain LeratAnne-Marie Simao-BeaunoirCarole Beaulieu Source Type: research
Proteomic analysis of Cucumis sativus cotyledons after glucohexaose treatment as a part of ROS accumulation related resistance mechanism.
Glucohexaose is a safe farm chemical used for pathogen prevention, which can induce systemic acquired resistance in cucumber. We found that glucohexaose treatment of cucumber plant induced an accumulation of the reactive oxidative species (ROS). Histochemistry showed sharp increases in O2- and H2O2 5h after glucohexaose treatment. After 5 h, the O2- content decreased to a normal level, but the H2O2 content remained at a high level 10 h after glucohexaose treatment. And antioxidant enzymes were also changed after glucohexaose treatment. We also investigated the relationship between ROS accumulation and glucohexaose-induced...
Source: Proteome Science - June 17, 2014 Category: Bioinformatics Authors: Yuhan HaoChunmei LinHaiyan FanYang YuNing LiShaoli Chen Source Type: research
Examination of the leaf proteome during flooding stress and the induction of programmed cell death in maize
Conclusions:
Following flooding stress, the photosynthetic systems were damaged, resulting in a disruption in energy metabolism, with the noted photosynthetic decline also possibly attributed to ROS production. The observed PCD could be regulated by TCTP with a possible role for H2O2 in TCTP induction under flooding stress. Additionally, increased SAMS2 expression was closely associated with an increased polyamine synthesis during flooding treatment. (Source: Proteome Science)
Source: Proteome Science - June 11, 2014 Category: Bioinformatics Authors: Yu ChenXi ChenHongjuan WangYiqun BaoWei Zhang Source Type: research
Inflammation: an important parameter in the search of prostate cancer biomarkers
Conclusions:
The present study indicates that inflammation might be a confounding parameter during the proteomic research of candidate biomarkers of PCa. These results indicate that some possible biomarker-candidate proteins are strongly influenced by the presence of inflammation, hence only a well-selected protein pattern should be considered for potential marker of PCa. (Source: Proteome Science)
Source: Proteome Science - June 9, 2014 Category: Bioinformatics Authors: Stefania BergaminiElisa BelleiLuca Reggiani BonettiEmanuela MonariAurora CuoghiFrancesco BorelliMaria SighinolfiGiampaolo BianchiTomris OzbenAldo Tomasi Source Type: research
Protein phosphorylation differs significantly among ontogenetic phases in Malus seedlings
Conclusions:
Protein phosphorylation varied significantly during vegetative phase change and floral transition in apple seedlings. Most of the observed changes were consistent among seedlings and between hybrid populations. (Source: Proteome Science)
Source: Proteome Science - May 24, 2014 Category: Bioinformatics Authors: Yan WangYi WangYong Bo ZhaoDong Mei ChenZhen Hai HanXin Zhong Zhang Source Type: research
Pepsin-pancreatin protein hydrolysates from extruded amaranth inhibit markers of atherosclerosis in LPS-induced THP-1 macrophages-like human cells by reducing expression of proteins in LOX-1 signaling pathway
Conclusions:
Extruded amaranth hydrolysate showed potential anti-atherosclerotic effect in LPS-induced THP-1 human macrophage-like cells by reducing the expression of proteins associated with LOX-1 signaling pathway. (Source: Proteome Science)
Source: Proteome Science - May 19, 2014 Category: Bioinformatics Authors: Alvaro Montoya-RodríguezJorge Milán-CarrilloVermont DiaCuauhtémoc Reyes-MorenoElvira González de Mejía Source Type: research
The Lottia gigantea shell matrix proteome: re-analysis including MaxQuant iBAQ quantitation and phosphoproteome analysis
Conclusions:
We show that iBAQ quantitation may be a useful tool to narrow down the group of functional biomineral matrix protein candidates for further research in cell biology, genetics or materials research. Knowledge of posttranslational modifications in these major proteins could be a valuable addition to previously published proteomes. This is true especially for phosphorylation, because this modification was already shown to modify mineralization processes in some instances. (Source: Proteome Science)
Source: Proteome Science - May 18, 2014 Category: Bioinformatics Authors: Karlheinz MannEric Edsinger Source Type: research
Assessment of vascular endothelial growth factor in formalin fixed, paraffin embedded colon cancer specimens by means of a well-based reverse phase protein array
Conclusions:
The well-based RPPA method is a high throughput and sensitive approach, is an excellent tool for quantification of marker proteins. Notably, this method may be helpful for more objective evaluation of protein expression in cancer patients. (Source: Proteome Science)
Source: Proteome Science - May 13, 2014 Category: Bioinformatics Authors: Joon-Yong ChungTill BraunschweigSeung-Mo HongDavid KwonSoo-Heang EoHyungJun ChoStephen Hewitt Source Type: research
Forced expression of S100A10 reduces sensitivity to oxaliplatin in colorectal cancer cells
Conclusions:
The present results have shown that protein expression of S100A10 was associated with resistance to L-OHP, but not 5-FU, supporting the hypothesis that S100A10 expression may predict L-OHP sensitivity. Thus, our present study provides basic findings to support that S100A10 expression can be used as a predictive marker for tumor sensitivity to L-OHP. (Source: Proteome Science)
Source: Proteome Science - May 9, 2014 Category: Bioinformatics Authors: Sayo SuzukiYusuke Tanigawara Source Type: research
Recovery of brain biomarkers following peroxisome proliferator-activated receptor agonist neuroprotective treatment before ischemic stroke
Conclusions:
Pre-treatment with PPARalpha agonist or atorvastatin show potential neuroprotective effects by inhibiting the PDI overexpression in conjunction with the preservation of other neuronal markers, several of which are associated with the regulation of protein homeostasis, signal transduction and maintenance of synaptic plasticity. This proteomic study therefore suggests that neuroprotective effect of PPARalpha agonists supposes the preservation of the expression of several proteins essential for the maintenance of protein homeostasis not necessarily directly linked to PPARalpha known-regulated targets. (Source: Proteome Science)
Source: Proteome Science - May 6, 2014 Category: Bioinformatics Authors: Patrick GeléValérie VingtdeuxCamille PoteyHervé DrobecqAntoine GhestemPatricia MelnykLuc BuéeNicolas SergeantRégis Bordet Source Type: research
Quantitative proteomics analysis of early recurrence/metastasis of huge hepatocellular carcinoma following radical resection
Background:
Hepatic resection is the preferred treatment for huge hepatocellular carcinoma (>10 cm in diameter; H-HCC). However, the patients with H-HCC suffer from poor prognosis due to the early recurrence/metastasis. The underlying mechanism of H-HCC's early recurrence/metastasis is currently not well understood.
Results:
Here, we describe an Isobaric Tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics approach to analyze the early recurrence/metastasis related proteins of H-HCC after radical resection through multidimensional chromatography coupled with tandem mass spectrometry (2DLC-MS/...
Source: Proteome Science - May 1, 2014 Category: Bioinformatics Authors: Xinhui HuangYongyi ZengXiaohua XingJinhua ZengYunzhen GaoZhixiong CaiBo XuXiaolong LiuAimin HuangJingfeng Liu Source Type: research
Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions
Conclusions:
The reported data suggest that while the general metabolic function of the sessile bacteria is minimal under high fluid shear stress conditions, they seem to retain the binding capacity to initiate new infections. (Source: Proteome Science)
Source: Proteome Science - April 30, 2014 Category: Bioinformatics Authors: Nazrul IslamYonghyun KimJulia RossMark Marten Source Type: research
