Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma
Conclusions:
In summary, the current study used a comprehensive lung adenocarcinoma-based proteomic approach for the identification of GR-modulated protein expression in response to UVB-irradiation. To our knowledge, this is the first global proteomic analysis to investigate the role of GR under UVB-irradiation in mammalian cell model. (Source: Proteome Science)
Source: Proteome Science - January 9, 2014 Category: Bioinformatics Authors: Hsiu-Chuan ChouHong-Lin Chan Source Type: research
2D-DIGE proteome analysis on the platelet proteins of patients with major depression
Conclusions:
Platelet provided valuable information about the pathways and processes of inflammation/immunity, oxidative stress, and neurogenesis, related to major depression. (Source: Proteome Science)
Source: Proteome Science - January 3, 2014 Category: Bioinformatics Authors: Tiao-Lai HuangMei-Lan SungTai-Yuan Chen Source Type: research
Exploring the ¿dark matter¿ of a mammalian proteome by protein structure and function modeling
Conclusions:
These results strongly indicate that many small proteins adopt three-dimensional structures and are fully functional, playing important roles in transcriptional regulation, cell signaling and metabolism. Data collected through this work is freely available to the academic community at http://www.brylinski.org/content/databases to support future studies oriented on elucidating the functions of hypothetical small proteins. (Source: Proteome Science)
Source: Proteome Science - December 9, 2013 Category: Bioinformatics Authors: Michal Brylinski Source Type: research
Exploring the "dark matter" of a mammalian proteome by protein structure and function modeling
Conclusions:
These results strongly indicate that many small proteins adopt three-dimensional structures and are fully functional, playing important roles in transcriptional regulation, cell signaling and metabolism. Data collected through this work is freely available to the academic community at http://www.brylinski.org/content/databases to support future studies oriented on elucidating the functions of hypothetical small proteins. (Source: Proteome Science)
Source: Proteome Science - December 9, 2013 Category: Bioinformatics Authors: Michal Brylinski Source Type: research
Comparative proteomic analysis of genetically modified maize grown under different agroecosystems conditions in Brazil
Conclusions:
To the best of our knowledge this study represents the first evidence of protein identities with differentially expressed isoforms in Brazilian MON810 genetic background hybrid grown under field conditions. As global databases on outputs from "omics" analysis become available, these could provide a highly desirable benchmark for safety assessments. (Source: Proteome Science)
Source: Proteome Science - December 4, 2013 Category: Bioinformatics Authors: Sarah Agapito-TenfenMiguel GuerraOdd-Gunnar WikmarkRubens Nodari Source Type: research
Surface-exposed proteins of pathogenic mycobacteria and the role of cu-zn superoxide dismutase in macrophages and neutrophil survival
Pathogenic mycobacteria are important agents causing human disease. Mycobacterium avium subsp. hominissuis (M. avium) is a species of recalcitrant environmental pathogen. The bacterium forms robust biofilms that allow it to colonize and persist in austere environments, such as residential and commercial water systems. M. avium is also an opportunistic pathogen that is a significant source of mortality for immune-compromised individuals. Proteins exposed at the bacterial surface play a central role in mediating the relationship between the bacterium and its environment. The processes underlying both biofilm formation and pa...
Source: Proteome Science - November 28, 2013 Category: Bioinformatics Authors: Michael McNamaraShin-Cheng TzengClaudia MaierMartin WuLuiz Bermudez Source Type: research
The Aeromonas salmonicida subsp. salmonicida exoproteome: global analysis, moonlighting proteins and putative antigens for vaccination against furunculosis
Conclusions:
In this part of our work we elucidated the whole in vitro exoproteome of hypervirulent A. salmonicida subsp. salmonicida and showed the secretion of several highly conserved cytoplasmic proteins with putative moonlighting functions and roles in virulence. All together, our results offer new information about the pathogenesis of furunculosis and point out potential candidates for vaccine development. (Source: Proteome Science)
Source: Proteome Science - October 15, 2013 Category: Bioinformatics Authors: Philippe Vanden BerghManfred HellerSophie Braga-LagacheJoachim Frey Source Type: research
Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients
Conclusions:
The present study reveals differences in protein abundance between medial/lateral femur condyles in OA patients. These regulatory differences expand the knowledge regarding OA disease markers and mechanisms. (Source: Proteome Science)
Source: Proteome Science - October 4, 2013 Category: Bioinformatics Authors: Johan StenbergUlla RüetschiEva SkiöldebrandJohan KärrholmAnders Lindahl Source Type: research
The Aeromonas salmonicida subsp. salmonicida exoproteome: determination of the complete repertoire of Type-Three Secretion System effectors and identification of other virulence factors
Conclusions:
We demonstrated the whole in vitro secretome and T3SS repertoire of hypervirulent A. salmonicida. Several toxins, adhesins and enzymes that are not part of the T3SS secretome were secreted to a higher extent in the extremely low-virulent DeltaascV mutant. All together, our results show the high importance of an intact T3SS to initiate the furunculosis and offer new information about the pathogenesis. (Source: Proteome Science)
Source: Proteome Science - September 27, 2013 Category: Bioinformatics Authors: Philippe Vanden BerghManfred HellerSophie Braga-LagacheJoachim Frey Source Type: research
Proteomic analysis of Fusarium oxysporum f. sp. cubense tropical race 4-inoculated response to Fusarium wilts in the banana root cells
Conclusions:
This report is the first to use proteomic profiling to study the molecular mechanism of banana roots infected with Foc4. The differentially regulated proteins involved in different defense pathways are likely associated with different resistant levels of the three banana cultivars. (Source: Proteome Science)
Source: Proteome Science - September 26, 2013 Category: Bioinformatics Authors: Xingshen LiTingting BaiYunfeng LiXiaolei RuanHuaping Li Source Type: research
The proteome of the calcified layer organic matrix of turkey (Meleagris gallopavo) eggshell
Conclusions:
Even members of the same avian family show important differences in eggshell matrix composition and more studies on the proteome and the transcriptome level will be necessary to identify a common toolkit of eggshell mineralization and to work out species differences among functional eggshell protein sets and their role in eggshell production. (Source: Proteome Science)
Source: Proteome Science - August 27, 2013 Category: Bioinformatics Authors: Karlheinz MannMatthias Mann Source Type: research
Potential biomarkers for adult acute myeloid leukemia minimal residual disease assessment searched by serum peptidome profiling
Conclusions:
We speculate the peptides can be used as potential markers for monitoring minimal residual disease and clinical outcome assessment. (Source: Proteome Science)
Source: Proteome Science - August 3, 2013 Category: Bioinformatics Authors: Ju BaiAili HeWanggang ZhangChen HuangJuan YangYun YangJianli WangYang Zhang Source Type: research
Label-free mass spectrometry proteome quantification of human embryonic kidney cells following 24 hours of sialic acid overproduction
Conclusions:
In this experiment we show that increased metabolic flux through sialic acid producing pathway affects the abundance of several protein transport, epithelial adherens junction, signaling and metabolic pathway related proteins. (Source: Proteome Science)
Source: Proteome Science - August 1, 2013 Category: Bioinformatics Authors: Ville ParviainenSakari JoenvääräNiina TohmolaRisto Renkonen Source Type: research
Differential protein expression in perfusates from metastasized rat livers
Conclusions:
These results show that perfusate proteome can be used as an alternative initial resource for biomarker identification, which ultimately requires validation in serum. (Source: Proteome Science)
Source: Proteome Science - July 29, 2013 Category: Bioinformatics Authors: Yang ZhangMenglin LiLilong WeiLisi ZhuSiqi HuShuzhen WuSucan MaYouhe Gao Source Type: research
Comparison of first dimension IPG and NEPHGE techniques in two-dimensional gel electrophoresis experiment with cytosolic unfolded protein response in Saccharomyces cerevisiae
Conclusions:
Comparison of broad range (pH 3--10) gradient-based 2DE methods suggests that NEPHGE-based method is preferable over IPG (Invitrogen) 2DE method for the analysis of basic proteins. Nevertheless, the narrow range (pH 4--7) IPG technique is a method of choice for the analysis of acidic proteins. (Source: Proteome Science)
Source: Proteome Science - July 27, 2013 Category: Bioinformatics Authors: Rimantas SlibinskasRaimundas Ra¿anskasR¿ta Zinkevi¿i¿t¿Evaldas ¿iplys Source Type: research
