Pre-analytical stability of the plasma proteomes based on the storage temperature
Conclusions:
Although there are many studies concerning protein stability of clinical samples during storage these findings may help to provide a better understanding of the changes of proteins induced by storage temperature. (Source: Proteome Science)
Source: Proteome Science - March 21, 2013 Category: Bioinformatics Authors: Sara PasellaAngela BarallaElisabetta CanuSara PinnaJames VaupelMarta DeianaClaudio FranceschiGiovannella BaggioAngelo ZinelluSalvatore SotgiaGiuseppe CastaldoCiriaco CarruLuca Deiana Source Type: research
Monitoring post mortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate
Conclusions:
Muscle exudate provided valuable information about the pathways and processes underlying the post mortem ageing period, highlighting the importance of post mortem modification of proteins and their interaction for the development of meat quality traits. (Source: Proteome Science)
Source: Proteome Science - March 20, 2013 Category: Bioinformatics Authors: Alessio Di LucaGiuliano EliaAnne MullenRuth Hamill Source Type: research
Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86
Conclusions:
Although post-anthesis temperature and fertilizer have very different effects on grain development and yield, the two treatments elicit surprisingly similar effects on the accumulation of gluten proteins. The similarity of the responses to the different treatments is likely due to source-sink activities of nitrogen reserves in the wheat plant. Because each protein that showed a response in this study is linked to a gene sequence, the work sets the stage for transgenic studies that will better elucidate the roles of specific proteins in flour quality and in the response to the environment. (Source: Proteome Science)
Source: Proteome Science - February 22, 2013 Category: Bioinformatics Authors: William HurkmanCharlene TanakaWilliam VenselRoger ThilmonySusan Altenbach Source Type: research
Proteins from Tuber magnatum Pico fruiting bodies naturally grown in different areas of Italy
Conclusions:
The results indicate that proteomic analysis is a suitable method for characterizing those differences occurring in samples and induced by the different environmental conditions present in the various Italian areas where T. magnatum can grow. The positive protein identification by MS analysis has proved that this method can be applied with success even in a species whose genome, at the moment, has not been sequenced. (Source: Proteome Science)
Source: Proteome Science - February 1, 2013 Category: Bioinformatics Authors: Federico VitaValentina LucarottiEmanuele AlpiRaffaella BalestriniAntonietta MelloAngela BachiMassimo AlessioAmedeo Alpi Source Type: research
Identification of differentially expressed proteins of Arthrospira (Spirulina) plantensis-YZ under salt-stress conditions by proteomics and qRT-PCR analysis
Arthrospira (Spirulina) platensis as a representative species of cyanobacteria has been recognized and used worldwide as a source of protein in the food, which possesses some unusual and valuable physiological characteristics, such as alkali and salt tolerance. Based on complete genome sequencing of Arthrospira (Spirulina) plantensis-YZ, we compared the protein expression profiles of this organism under different salt-stress conditions (i.e. 0.02 M, 0.5 M and 1.0 M NaCl, respectively), using 2-D electrophoresis and peptide mass fingerprinting, and retrieved 141 proteins showing significantly differential expression in resp...
Source: Proteome Science - January 30, 2013 Category: Bioinformatics Authors: Huili WangYanmei YangWei ChenLi DingPeizhen LiXiaokai ZhaoXuedong WangAiying LiQiyu Bao Source Type: research
On protein abundance distributions in complex mixtures
Mass spectrometry, an analytical technique that measures the mass-to-charge ratio of ionized atoms or molecules, dates back more than 100 years, and has both qualitative and quantitative uses for determining chemical and structural information. Quantitative proteomic mass spectrometry on biological samples focuses on identifying the proteins present in the samples, and establishing the relative abundances of those proteins. Such protein inventories create the opportunity to discover novel biomarkers and disease targets. We have previously introduced a normalized, label-free method for quantification of protein abundances u...
Source: Proteome Science - January 29, 2013 Category: Bioinformatics Authors: JA KoziolNM GriffinF LongY LiM LatterichJE Schnitzer Source Type: research
Comparative proteomic analysis implicates eEF2 as a novel target of PI3K¿ in the MDA-MB-231 metastatic breast cancer cell line
Conclusions:
Our data imply a novel role for PI3Kγ in facilitating cell migration by regulating phosphorylation of eEF2. (Source: Proteome Science)
Source: Proteome Science - January 15, 2013 Category: Bioinformatics Authors: Meizhi NiuManuela Klingler-HoffmannJulie BrazzattiBriony ForbesChareeporn AkekawatchaiPeter HoffmannShaun McColl Source Type: research
Major soluble proteome changes in Deinococcus deserti over the earliest stages following gamma-ray irradiation
Conclusions:
Post-translational modifications of GyrA (N-terminal methionine removal and acetylation) have been evidenced and their significance discussed. We found that the Deide_02842 restriction enzyme, which is specifically found in D. deserti, is a new potential member of the radiation/desiccation response regulon, highlighting the specificities of D. deserti compared to the D. radiodurans model. (Source: Proteome Science)
Source: Proteome Science - January 15, 2013 Category: Bioinformatics Authors: Alain DedieuElodie SahinovicPhilippe GuérinLaurence BlanchardSylvain FochesatoBruno MeunierArjan de GrootJean Armengaud Source Type: research
Comparative proteomic analysis implicates eEF2 as a novel target of PI3Kgamma in the MDA-MB-231 metastatic breast cancer cell line
Conclusions:
Our data imply a novel role for PI3Kgamma in facilitating cell migration by regulating phosphorylation of eEF2. (Source: Proteome Science)
Source: Proteome Science - January 15, 2013 Category: Bioinformatics Authors: Meizhi NiuManuela Klingler-HoffmannJulie BrazzattiBriony ForbesChareeporn AkekawatchaiPeter HoffmannShaun McColl Source Type: research
Unrestrictive identification of post-translational modifications in the urine proteome without enrichment
Conclusions:
In this study, we identified PTMs of urine proteins without the need for enrichment. Our investigation may provide a useful reference for biomarker discovery in the future. (Source: Proteome Science)
Source: Proteome Science - January 14, 2013 Category: Bioinformatics Authors: Liu LiuXuejiao LiuWei SunMingxi LiYouhe Gao Source Type: research
Improved spatial learning and memory by perilla diet is correlated with immunoreactivities to neurofilament and ¿-synuclein in hilus of dentate gyrus
Background:
Perilla (Perilla frutescens) oil is very rich in α-linolenic acid, an omega-3 fatty acid. As it is widely reported that omega-3 fatty acid supplementation improves cognitive function in children and adults, feeding rats with perilla diets followed by analysis of proteomic changes in the hippocampus can provide valuable information on the mechanism of learning and memory at the molecular level. To identify proteins playing roles in learning and memory, differentially expressed proteins in the hippocampus of the 5 week old rats fed perilla diets for 3 weeks or 3 months were identified by proteomic analysis and v...
Source: Proteome Science - December 5, 2012 Category: Bioinformatics Authors: Jinwoo LeeSunmin ParkJu-Young LeeYeong Keun YeoJong Sang KimJinkyu Lim Source Type: research
Erratum to: A comparison of imputation procedures and statistical tests for the analysis of two-dimensional electrophoresis data
(Source: Proteome Science)
Source: Proteome Science - March 29, 2011 Category: Bioinformatics Authors: Jeffrey C Miecznikowski, Senthilkumar Damodaran, Kimberly F Sellers, Donald E Coling, Richard Salvi and Richard A Rabin Source Type: research
Publishing proteomic data
Scientific publications should provide sufficient detail in terms of methodology and presented data to enable the community to reproduce the methodology to generate similar data and arrive at the same conclusi... (Source: Proteome Science)
Source: Proteome Science - April 28, 2006 Category: Bioinformatics Authors: Martin Latterich Source Type: research
Molecular systems biology at the crossroads: to know less about more, or to know more about less?
Systems biology is a rapidly evolving discipline that endeavours to understand the detailed coordinated workings of entire organisms, with the ultimate goal to detect differences between health and disease, or... (Source: Proteome Science)
Source: Proteome Science - October 28, 2005 Category: Bioinformatics Authors: Martin Latterich Source Type: research
