Fast fixing and comprehensive identification to help improve real-time ligands discovery based on formaldehyde crosslinking, immunoprecipitation and SDS-PAGE separation
Conclusions:
Fast cross-linking was achieved. The 4 F strategy can be used to identify real-time in situ interactions without prior intervention and to comprehensively identify ligands of particular target proteins with fewer false positives.
Source: Proteome Science - Category: Bioinformatics Authors: Lisi ZhuMenglin LiLilong WeiXuejiao LiuJianrui YinYouhe Gao Source Type: research