Analysis of the subcellular location of lncRNA SLC16A1-AS1 and its interaction with premature miR-5088-5p in oral squamous cell carcinoma
AbstractSLC16A1-AS1 is a newly identified lncRNA with different roles in different cancers. MiR-5088-5p is an oncogenic miRNA in breast cancer. However, their participation in oral squamous cell carcinoma (OSCC) is unknown. We predicted the interaction between SLC16A1-AS1 and miR-5088-5p, and this study was carried out to explore the crosstalk between them in OSCC. A total of 56 OSCC patients donated OSCC and paired non-tumor tissues, which were used to detect the differential expression of SLC16A1-AS1 and miR-5088-5p (mature and premature). Analysis of the subcellular location of SLC16A1-AS1 in OSCC cells and its direct i...
Source: Odontology - July 12, 2022 Category: Dentistry Source Type: research
Neural network approach to evaluate the physical properties of dentin
This study intended to evaluate the effects of inorganic trace elements such as magnesium (Mg), strontium (Sr), and zinc (Zn) on root canal dentin using an Artificial Neural Network (ANN). The authors obtained three hundred extracted human premolars from type II diabetic individuals and divided them into three groups according to the solutions used (Mg, Sr, or Zn). The authors subdivided the specimens for each experimental group into five subgroups according to the duration for which the authors soaked the teeth in the solution: 0 (control group), 1, 2, 5, and 10 min (n = 20). The authors then tested the specimens fo...
Source: Odontology - July 10, 2022 Category: Dentistry Source Type: research
Effects of continuous chelation on the instrumentation of curved canals with a martensitic NiTi file system: an ex vivo micro-CT evaluation
This study aimed to evaluate the influence of continuous chelation on apical transportation and centrality in shaped curved canals prepared with a martensitic file system and evaluated by micro-CT. Twenty-four lower molar canals with a curvature of 25 º–40º were scanned pre-operatively with micro-CT and divided into 2 groups according to two different irrigation regimens (n = 12). Group 1 was irrigated with NaOCl, group 2 with Dual Rinse. All canals were prepared with VDW.ROTATE system. The samples were scanned post-operatively and evaluated at 1, 3 and 5 mm from the apex to determine apical transportation and can...
Source: Odontology - July 7, 2022 Category: Dentistry Source Type: research
Regeneration of periodontal bone defects with mesenchymal stem cells in animal models. Systematic review and meta-analysis
AbstractThe aim of this study was to evaluate the efficacy of mesenchymal stem cells (MSCs) in the regeneration of periodontal bone defects in animal models. A systematic review and meta-analysis were conducted following the PRISMA guidelines, and the study was recorded in PROSPERO under reference number CDR42021247462. The PICO question was: is periodontal regeneration (cementum, periodontal ligament and alveolar bone) with MSCs more effective than other techniques? Three groups were considered: Group 1: MSCs alone or mixed with regenerative materials. Group 2: only regenerative materials. Group 3: no regenerative materia...
Source: Odontology - July 5, 2022 Category: Dentistry Source Type: research
The relationship between FOSB and SOCS3 gene polymorphisms and the susceptibility to periodontitis and osteopenia in the Chinese population
AbstractThe aim is to analyze the relationship and significance of theFOS,FOSB, Suppressors of cytokine signaling (SOCS), and hypoxia-inducible factor 1 (HIF1) gene loci and their polymorphisms with periodontitis and osteopenia in the Chinese population. In this case –control study, data on questionnaires, periodontal examination, bone mineral density, andFOS,FOSB,SOCS3, and HIF1 gene loci and their polymorphisms were obtained from 474 participants. The data were analyzed using the analysis of variance, Kruskal –Wallis test,χ2 test, and logistic regression. The incidence of osteopenia was significantly increased in pa...
Source: Odontology - June 4, 2022 Category: Dentistry Source Type: research
The optimization of ligature/bone defect-induced periodontitis model in rats
In this study, we created periodontitis rat models in silk ligation group, bone defect group and bone defect/silk ligation group, respectively. Obvious periodontal inflammation but slight alveolar bone resorption was observed in the ligation group, while surgical trauma was not robust enough to continually worsen the constructed bone defect area in the bone defect group. In the bone defect/ligature group, significant and stable periodontal inflammation was the most enduring with similar evolving pathological patterns of human periodontitis. It also exhibited enhanced clinical similarity and confirmed its superiority in qua...
Source: Odontology - June 3, 2022 Category: Dentistry Source Type: research
Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction
AbstractIn the current study, we explored the role of Mg2+-doped CaSO4/ β-TCP composite biopolymer in regulating macrophage polarization and its relation with enhanced osteogenic differentiation of periodontal ligament stem cells. Furthermore, mechanism underling the regulation of macrophage polarization by CaSO4/ β-TCP was evaluated. Mg2+-doped CaSO4/ β-TCP composite was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Macrophage polarization was characterized using flow cytometry analysis. Macrophage morphometric analysis was conducted by FITC phalloidin staining. Western blot and qRT-P...
Source: Odontology - June 2, 2022 Category: Dentistry Source Type: research
Orofacial problems in scuba diving: prevalence and prevention —a large-scale survey among civilian divers in France
In conclusion, oral problems in scuba diving represent frequent events that can compromise the safety of divers. Despite awareness rising, there is a lack of recourse to the dentist. This leads to incomplete information, especially concerning the preventive means available to divers. (Source: Odontology)
Source: Odontology - May 30, 2022 Category: Dentistry Source Type: research
The association between dental caries and physical activity, physical fitness, and background factors among Finnish male conscripts
AbstractStudies on measured physical fitness and oral health are sparse. The aim of this study was to investigate the associations between self-reported physical activity and measured physical fitness and oral health of young men. The study population consisted of 13,564 Finnish male conscripts who had mandatory clinical oral examinations and physical fitness tests at the beginning of military service in 2011. Finally, around 10,800 conscripts had physical fitness test outcomes available and a total of 8552 conscripts answered a computer-based questionnaire on background factors. Decayed Tooth (DT) and Decayed, Missing, or...
Source: Odontology - May 25, 2022 Category: Dentistry Source Type: research
External validation of cone-beam computed tomography- and panoramic radiography-featured prediction models for inferior alveolar nerve injury after lower third molar removal: proposal of a risk calculator
AbstractWe previously developed basic and extended models to predict inferior alveolar nerve injuries (IANI) after lower third molar (LM3) removal based on cone-beam computed tomography (CBCT) images. Although these models comprised predictors, including increased age and inferior alveolar canal-related CBCT factors, external validations were lacking. Therefore, this study externally validated these models and compared them with other related models based on their performance. Original and newly validated samples included patients who underwent LM3 removal following CBCT. Subsequently, 39 and 25 patients with IANI, then 45...
Source: Odontology - May 23, 2022 Category: Dentistry Source Type: research
Root canal narrowing patterns of mandibular first premolars on panoramic radiographs according to the number of root canals diagnosed on cone beam computed tomographic images
This study aimed to investigate the characteristic radiographic findings of mandibular first premolars possessing multiple root canals and determine the diagnostic accuracy using panoramic radiography. Four radiologists evaluated 347 mandibular first premolars in 186 patients who underwent panoramic radiography and dental cone beam computed tomography (CBCT). Two radiologists confirmed the existence of single or multiple root canals using CBCT. The remaining two radiologists evaluated the imaging findings of the root canal morphology and classified them into two patterns: gradual narrowing and sudden narrowing. In the case...
Source: Odontology - May 12, 2022 Category: Dentistry Source Type: research
Management of fear and anxiety in dental treatments: a systematic review and meta-analysis of randomized controlled trials
ConclusionsInterventions should be used in managing anxiety and fear for dental treatment. It might be effective for anxiety alleviating for adults, but there was a low certainty of evidence that interventions could reduce anxiety level in children and adolescents. Sedation drugs and audio –visual distractions might be useful for managing dental fear and anxiety. Pain levels and vital signs could not be improved form our study. High-quality randomized clinical trials are required for further study. (Source: Odontology)
Source: Odontology - May 10, 2022 Category: Dentistry Source Type: research
Vertical root fracture resistance and crack formation of root canal-treated teeth restored with different post-luting systems
AbstractThe aim was to investigate the vertical root fracture (VRF) resistance and crack formation of root canal-treated teeth restored with different post-luting systems. Human maxillary lateral incisors of similar size were decoronated, assigned to five groups (n = 18, power = 0.9) and embedded in acrylic blocks with artificial periodontal ligament. After root canal filling, post spaces were prepared to place coated fiber-reinforced composite (FRC) or sandblasted titanium (Ti) posts of the same shape and size. Half of the posts were zinc phosphate c emented (C), while the other half was adhesively luted (A). Untr...
Source: Odontology - May 6, 2022 Category: Dentistry Source Type: research
Evaluation of a hyaluronic acid hydrogel (Restylane Lyft) as a scaffold for dental pulp regeneration in a regenerative endodontic organotype model
This study aimed to evaluate the effect of an injectable synthetic scaffold (Restylane Lyft) on human bone marrow mesenchymal stem cell (hBMSC) viability, proliferation, and osteo/dentinogenic differentiation in a regenerative endodontic organotype model (REM). hBMSC were loaded in an REM either alone (hBMSC group) or mixed with the Restylane Lyft scaffold (Restylane/hBMSC group) and cultured in basal culture medium (n = 9/group). hMSC on culture plates served as controls. Cell viability and proliferation were measured using AlamarBlue assay. The loaded REM was cultured in an osteogenic differentiation medium to measur...
Source: Odontology - April 26, 2022 Category: Dentistry Source Type: research
Does the use of fiber posts increase the fracture resistance of simulated immature teeth? A systematic review
AbstractThis systematic review aimed to assess if the use of fiber posts reinforces weakened immature teeth. A systematic review was conducted of laboratory studies that evaluated the fracture resistance of simulated immature teeth restored with fiber posts compared to teeth restored exclusively with resin. An electronic search was performed using the following databases: PubMed/MEDLINE, Web of Science, Scopus and LILACS, BBO, and grey literature. Two independent researchers screened the titles and abstracts of the retrieved studies for relevance to the research question. Subsequently, the full texts of potentially relevan...
Source: Odontology - April 20, 2022 Category: Dentistry Source Type: research
