How do carious root lesions develop after the end of professional preventive measures? —Preliminary findings of a randomized clinical trial
AbstractAim of this randomized clinical trial was to assess the development of root caries lesions with and without (adjuvant) professional prevention treatment over 24 months. 20 participants with two or three non-cavitated root carious lesions were included (n = 52), whereby lesions were randomly assigned to one out of three groups depending on varnish application (CF: Cervitec F [n = 20], P: placebo [n = 20], DP: Duraphate [n = 12]). All lesions were assessed by quantitative light-induced fluorescence (QLF; QRayCam); following outcome parameters were analyzed: fluorescence loss (ΔF %), lesion volume (...
Source: Odontology - April 11, 2022 Category: Dentistry Source Type: research
Inflammatory activity and apoptosis are associated with tissue degeneration in the submandibular gland of rats submitted to paradoxical sleep deprivation
AbstractThe aim of this study was to evaluate if paradoxical sleep deprivation is able to induce tissue degeneration, inflammatory activity and apoptosis in the submandibular gland of rats. A total of 24 male Wistar rats were distributed into the following groups: group 1-control (CTRL;n = 8): the animals were not submitted to any procedures; group 2-sleep deprivation (PS;n = 8): the animals were submitted to paradoxical sleep deprivation for 96 h and group 3-recovery (R;n = 8): the animals were submitted to sleep deprivation for 96 h, followed by a period of 96 h without any intervention. The following para...
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
Microgap and microleakage of a hybrid connection platform-switched implant system in the absence or presence of a silicone-based sealing agent
AbstractThe present study aims to characterize, for the first time, the microgap and bacterial microleakage of a platform-switched implant system with hybrid connection, screwed at distinct torque values (manufacturer recommended torque —25 N cm—and a reduced torque—5 N cm—mimicking the long-term functional use), in the absence or presence of a silicon-based sealing agent. Microgap was determined through scanning electron microscopy and bacterial microleakage was evaluated in vitro, uponEnterococcus faecalis colonization of the system. The sealing efficacy was evaluated in the absence or presence of a commercia...
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
Effects of rice fermented extracts, “Sake Lees”, on the functional activity of odontoblast-like cells (KN-3 cells)
This study was designed to investigate the effects of Sake Lees extracts (SLE, Sake Kasu) on the functional activity of odontoblastic cells and tooth pulp of the rats. For in vitro studies, a rat clonal odontoblast-like cell line, KN-3 cells were cultured. SLE significantly decreased KN-3 cell proliferation, but showed no significant cytotoxicity. SLE effects on several protein productions of KN-3 cells were compared with PBS. SLE and PBS increased alkaline phosphatase (ALP), dentin sialoprotein (DSP), and osterix in a day-course dependent manner, while SLE increased the induction of ALP on day 9 –21 and DSP on day 15–...
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
Effects of phytosomal curcumin treatment on modulation of immunomodulatory and pulp regeneration genes in dental pulp mesenchymal stem cells
AbstractDental pulp stem cells (DPSCs) are a new population of mesenchymal stem cells (MSCs) located in the oral cavity with potential capacities for tissue regeneration and immunomodulation. The purpose from this study was to determine effects of curcumin nanoparticle into phytosomal formulation (PC) on the relative expression of DSPP, VEGF-A, HLA-G5, VCAM1, RelA and STAT3 genes which are among the most important factors influencing processes of immunomodulatory and tissue regenerative by DPSCs. After isolation and culture of DPSCs, these cells were characterized according to predetermined criteria including flow cytometr...
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
Fracture strength of extended class I composite restorations with different restorative techniques
AbstractTo evaluate the fracture strength of extended Class I restorations with different restorative techniques using nanofilled and nanohybrid composites. Sixty extracted human third molars were prepared with extended Class I cavities and divided into six groups: groups FS-F (Filtek bulk-fill Flow + Filtek Supreme Ultra, 3 M) and GR-F (X-tra base + GrandioSO, VOCO), restored with a flowable bulk-fill composite as a base covered by a nanofilled or nanohybrid composite; groups FB (Filtek One Bulk-Fill, 3 M) and AF (Admira Fusion X-tra, VOCO), restored with a bulk-fill resin composite ; and groups FS (Filtek Supr...
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
Occlusal problems, mental health issues and non-carious cervical lesions
AbstractNon-carious cervical lesions (NCCLs) are characterized by a loss of hard dental tissue near the cement –enamel junction with multifactorial etiology. The aim of this study was to demonstrate that occlusal factors as attrition, malocclusion, and bruxism, and mental disorders as depression, stress, and anxiety are involved in the etiology of NCCLs. Salivary samples and clinical data of 340 individual s selected from 6,112 participants were obtained from the University of Pittsburgh School of Dental Medicine Dental Registry and DNA Repository project. The affected group was formed by individuals with NCCL (34 female...
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
An in-vivo study of photobiomodulation using 403 nm and 649 nm diode lasers for molar tooth extraction wound healing in wistar rats
ConclusionThe red diode laser 649 nm has been shown to accelerate the process of proliferation in wound healing post molar extraction based on histopathological and immunohistochemical test results. (Source: Odontology)
Source: Odontology - March 18, 2022 Category: Dentistry Source Type: research
Efficacy of concentrated growth factor with low-level laser for the regeneration of interdental papilla defects
This study aims to evaluate the efficacy of regenerating interdental papilla by Liquid ph ase concentrated growth factor (LPCGF) injection with low-level-laser therapy (LLLT). (Source: Odontology)
Source: Odontology - March 15, 2022 Category: Dentistry Source Type: research
Postoperative pain after SWEEPS, PIPS, sonic and ultrasonic-assisted irrigation activation techniques: a randomized clinical trial
AbstractTo investigate the efficacy of a new laser irrigation activation system [shock wave-enhanced emission photo-acoustic streaming (SWEEPS)] in terms of postoperative pain after primary root canal treatment compared with other techniques, namely photon-induced photo-acoustic streaming (PIPS), sonic system with EDDY, passive ultrasonic system (PUI), and manual dynamic activation (MDA). Two hundred patients with symptomatic irreversible pulpitis in mandibular premolar teeth were enrolled and randomly allocated to five different irrigation activation groups (n = 40) after chemo-mechanical root canal preparation. For i...
Source: Odontology - March 10, 2022 Category: Dentistry Source Type: research
Repeatability of dental shade by digital spectrophotometry in current, former, and never smokers
AbstractCigarette smoking contributes to poor oral health and dental discoloration. Therefore, stopping smoking may translate into measurable amelioration of dental shade indices. We compared dental shade parameters by digital spectrophotometry among current, former, and never smokers and verified their repeatability at 7 and 30 days. Dental shade parameters (CIE L*a*b* and corresponding whiteness index for dentistry-WID) were measured in current, former, and never smokers with a digital spectrophotometer (Vita Easyshade V) on three separate study visits: at baseline (day 0), at day 7, and day 30. Dental shade parameters...
Source: Odontology - March 10, 2022 Category: Dentistry Source Type: research
How prenatal environmental factors affect rat molar enamel formation?
AbstractAmelogenin (AMELX) and ameloblastin (AMBN) are crucial for enamel formation, and interruptions in the production of these proteins may cause enamel defects. We investigated how prenatal environmental factors (chronic stress, bisphenol A (BPA), amoxicillin, and lipopolysaccharide (LPS)) affect AMELX and AMBN production of ameloblasts. Fifteen pregnant Sprague –Dawley rats were divided into four experimental groups and a control group. Chronic-stress group rats were exposed to a 12:12 light/light cycle (LL) from day E18 until delivery. BPA group rats were orally administered 5 μg/kg BPA daily from day E1 until de...
Source: Odontology - March 9, 2022 Category: Dentistry Source Type: research
The effect of diabetes on the tensile bond strength of a restorative dental composite to dentin
This study was performed to evaluate the effect of diabetes mellitus (DM) on the tensile bond strength (TBS) of dental composite resin bonding to enamel and dentin of extracted human teeth. Thirty caries-free human premolar teeth (10 from type 1 DM (D1), 10 from type 2 DM (D2), and 10 from non-diabetic individuals (control)) were wet ground and polished to obtain flat surfaces of dentin (n = 10). 37% phosphoric acid was used to etch dentin specimens for 15 s. After the application of the adhesive system, the composite resins were applied and cured for 20 s. Then, specimens were subjected to TBS testing by Universal T...
Source: Odontology - March 4, 2022 Category: Dentistry Source Type: research
Glass fiber posts relining: can composite opacity influence retention to root canal dentin?
This study aimed at evaluating the influence of glass-fiber post (GFP) relining with composites of different opacities on resin cement layer thickness (CLT), bond strength (BS) to root dentin, and resin cement degree of conversion (DC%). Standardized roots of 52 bovine incisors had their canals prepared and were distributed into 4 groups (n = 10 for CLT and BS;n = 3 for DC%) according to the post used: WP3 (Control)—Whitepost DC3; groups DE, EN and TR—Whitepost DC0.5 relined, respectively, with dentin, enamel, and translucent shade composites. After cementation, specimens were sectioned into six 1.0 mm-thick d...
Source: Odontology - March 1, 2022 Category: Dentistry Source Type: research
Upregulation of TRPA1 and reduction of NF- κB translocation could be part of the immunomodulatory process during primary tooth inflammation
This study aimed to better understand how TRPA1 responds to inflammation induced by bacterial-related stimuli during dental caries. The pulp of 40 primary teeth and 46 permanent teeth were categorized into three stages of carious progression: intact dentin, exposed dentin and exposed pulp. We measured the percentages of cells with NF- κB nuclear translocation to verify the severity of inflammation, and then assessed TRPA1 expression by immunofluorescence technique in the pulpal horn, subodontoblastic and mid-coronal regions of the dental pulp samples at various stages of caries. We found NF-κB nuclear translocation gradu...
Source: Odontology - February 28, 2022 Category: Dentistry Source Type: research
