Hsa_circ_0081069 facilitates tongue squamous cell carcinoma progression by modulating MAP2K4 expression via miR-634
AbstractIt has been demonstrated that circular RNA (circRNA) is involved in the progression of tongue squamous cell carcinoma (TSCC). The aim of this study was to investigate the intrinsic mechanism of circ_0081069 in TSCC progression. The expression levels of circ_00081069, miR-634, and mitogen-activated protein kinase kinase 4 (MAP2K4) in TSCC tissues and cells were detected by quantitative real-time PCR (qRT-PCR). Cell counting kit 8 assay, Edu assay, and flow cytometry assay were used to detect cell proliferation and cell cycle distribution. Transwell assay was used to detect cell migration and invasion abilities. West...
Source: Odontology - October 1, 2022 Category: Dentistry Source Type: research
Permeability of P. gingivalis or its metabolic products through collagen and dPTFE membranes and their effects on the viability of osteoblast-like cells: an in vitro study
AbstractMembrane exposure is a widely reported and relatively common complication in Guided Bone Regeneration (GBR) procedures. The introduction of micro-porous dPTFE barriers, which are impervious to bacterial cells, could reduce the technique sensitivity to membrane exposure, even if there are no studies investigating the potential passage of bacterial metabolites through the barrier. Aim of this study was the in vitro evaluation of the permeability of three different GBR membranes (dPTFE, native and cross-linked collagen membranes) toPorphyromonas gingivalis; in those cases, where bacterial penetration could not be obse...
Source: Odontology - September 10, 2022 Category: Dentistry Source Type: research
Effects of ultraviolet irradiation on beta-tricalcium phosphate as a bone graft substitute
AbstractSurface modification of various materials using ultraviolet (UV) irradiation improves their wettability. The purpose of this study was to investigate the wettability of a β-tricalcium phosphate (TCP) surface and the composition changes and bioactivity of β-TCP after UV irradiation. We applied 172 nm UV treatment to a β-TCP surface and measured the contact angle before and after UV irradiation. Energy-dispersive X-ray and Fourier transform infrared spectroscopy ex aminations were performed on the β-TCP disk with or without UV treatment. In an adhesion test of bone marrow cells using β-TCP disks with and withou...
Source: Odontology - September 10, 2022 Category: Dentistry Source Type: research
Effect of thickness on color appearance of multilayer CAD/CAM composite resin blocks
In conclusion, the co lor appearance of CAD/CAM blocks was significantly influenced by both the thickness and layer.L*a*b* decreased with thickness, and a negative exponential relationship between TP and thickness was observed for all layers and brands. (Source: Odontology)
Source: Odontology - September 10, 2022 Category: Dentistry Source Type: research
Gut flora alterations due to lipopolysaccharide derived from Porphyromonas gingivalis
AbstractGut dysbiosis induces ‘leaky gut,’ a condition associated with diabetes, NASH, and various auto-immune diseases.Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow. Oral administration ofP. gingivalis induced gut dysbiosis in mice model, but no systemic administration ofP. gingivalis has been reported thus far. In the present study, we investigated the effect ofP. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model. Eight-week-old C57BL/6J mice were intraperitoneally adminis...
Source: Odontology - September 10, 2022 Category: Dentistry Source Type: research
Comparison of the dimensional and morphological accuracy of three-dimensional digital dental casts digitized using different methods
AbstractThe purpose of this study was to compare the accuracy of digital dental casts from plaster cast scanning (PCS), impression scanning (IPS), intraoral scanning (IOS), and cone-beam computed tomography (CBCT) scanning (CCS) methods. The maxillary and mandibular dental casts of 15 patients who needed CBCT scans for oral examination or treatment were digitized via four methods. 12 linear distance measurements of all digital dental casts were selected and acquired with software and compared to those of the reference plaster cast to evaluate the dimensional accuracy. Three-dimensional deviation analysis of the IPS, IOS an...
Source: Odontology - September 7, 2022 Category: Dentistry Source Type: research
Modification of glass-ionomer cement properties by quaternized chitosan-coated nanoparticles
AbstractGlass ionomers (GICs), because of their qualities, are in a good position to be modified to resist masticatory stresses as permanent posterior restoration and prevent recurrent caries. The purpose of the present study was to evaluate the effect of adding quaternized chitosan-coated mesoporous silica nanoparticles (HTCC@MSNs) to conventional GIC on its mechanical properties, antimicrobial activity and fluoride release and the effect of 1- and 3-month water aging on the studied properties. HTCC@MSNs was synthesized, added to commercially available conventional GIC at 1%, 3%, and 5% by weight forming three experimenta...
Source: Odontology - September 7, 2022 Category: Dentistry Source Type: research
Long-term changes in oral feeding behaviors of growing rats
AbstractOral feeding is critical for survival in both humans and animals. However, few studies have reported quantitative behavioral measures associated with the development of oral feeding behaviors. Therefore, the present study investigated developmental changes in the oral feeding behaviors of rats by quantitatively assessing pasta eating and licking behaviors. In the pasta eating test, the time to finish pasta sticks of three different thicknesses ( Φ = 0.9, 1.4, and 1.9 mm, 4 cm long) was recorded between postnatal day 29 (P29) and P49, because all rats were able to finish eating these pasta sticks on P29. A de...
Source: Odontology - September 5, 2022 Category: Dentistry Source Type: research
0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats
This study was to investigate whether the programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) and T-helper 17 (Th17)/regulatory T (Treg) balance are associated with chronic apical periodontitis (CAP) relived by 0.1% nano-silver. CAP rat models were established by opening the first molars of the right and left mandible and exposing the pulp cavity to the oral cavity. CAP model was verified by cone-beam computed tomography, X-ray digital radiovisiography, and hematoxylin –eosin (H and E) staining. The rats were randomly divided into the sham, Ca(OH)2, and 0.1% nano-silver groups (n = 12 in each group) 2 weeks ...
Source: Odontology - September 4, 2022 Category: Dentistry Source Type: research
Cone beam computed tomography assessment of the prevalence and association of pulp calcification with periodontitis
In conclusion, the occurrence of PC is related to the periodontal state, and the prevalence of PC is higher in teeth with periodontitis; tooth type and periodontitis status are important risk factors for PC. (Source: Odontology)
Source: Odontology - August 14, 2022 Category: Dentistry Source Type: research
Alterations of posterior pharyngeal wall movement during swallowing in postoperative tongue cancer patients: assessment with a videofluoroscopic swallowing study
This study aimed to determine the association between the progressive contraction of the posterior pharyngeal wall and dysphagia in postoperative patients with tongue cancer. A videofluoroscopic swallowing study (VFSS) was performed in 34 patients after tongue cancer surgery. Images were analyzed using a two-dimensional video measurement software. Cases in which the processes on the posterior pharyngeal wall moved downward from the 2nd to 4th vertebral regions were defined as “normal type”, other cases were defined as “abnormal type”. Twenty-four patients showed normal movement of the posterior pharyngeal wall, whe...
Source: Odontology - August 11, 2022 Category: Dentistry Source Type: research
Accuracy of integration of dental cast and cephalograms compared with cone-beam computed tomography: a comparative study
This study proposes a method that integrates maxillary dental cast and cephalograms and evaluates its accuracy compared with cone-beam computed tomography (CBCT) scans. The study sample comprised 20 adult patients with records of dental casts, cephalograms, and craniofacial CBCT scans. The maxillary dental cast was integrated with lateral and frontal cephalograms based on best-fit registration of palatal and dental outline curves from dental cast with cephalogram tracings. Linear measurement was conducted to assess the intra- and inter-examiner reproducibility of the proposed integration method using intraclass correlation...
Source: Odontology - August 9, 2022 Category: Dentistry Source Type: research
Study of clinical factors, focus score, lymphocyte type and NF- κB pathway in Sjögren’s syndrome
In this study, we examined and com pared the results of comprehensive immunohistochemical staining of lymphocytes and NF-κB pathway in labial gland biopsies, clinical test data, and radionuclide imaging findings. One hundred labial gland biopsy specimens obtained from 20 female patients with primary SS (5 specimens per patient) were studied. Hematoxylin–eosin-stained specimens were reviewed and FS were calculated. Immunohistochemical staining of CD4, CD8, CD20, CD25, Foxp3, NF-κB, TNFAIP3 and IκBα was performed, and the results were compared with anti-SS-A/Ro (SS-A), anti-SS-B/La (SS-B) and antinuclear antibodies (AN...
Source: Odontology - August 2, 2022 Category: Dentistry Source Type: research
Comparison of heat production and bone architecture changes in the implant site preparation with compressive osteotomes, osseodensification technique, piezoelectric devices, and standard drills: an ex vivo study on porcine ribs
This study aimed at investigating differences in heat generation and bone architecture following four different implant site preparation techniques: compressive osteotomes, conventional drills, osseodensification (OD mode with osseodensification drills), and piezoelectric systems. Porcine rib bones were used as a model for implant surgery. Thermocouples were employed to measure temperature changes, and micro-CT to assess the bone architecture. The primary stability and insertion torque values of the implants placed in the differently prepared sites were assessed. The temperature changes were higher with Piezo. The average ...
Source: Odontology - July 19, 2022 Category: Dentistry Source Type: research
Effect of different irrigation activation techniques on irrigation penetration into the simulated lateral canals
This study, in which a hundred human teeth with single straight canal were used, focused on the evaluation of contrast solution penetration ability into the simulated lateral canals activated by XP-endo Finisher (XPF), EDDY, Nd:YAG, and Er:YAG laser systems with three different observation methods. The root canals were prepared up to X4 at working length using the ProTaper Next system. The teeth were decalcified and simulated lateral canals were created with #8 K-file at 2, 4, and 6 mm levels. Then the teeth were cleared using methyl salicylate and divided into 5 equal groups according to irrigation activation technique...
Source: Odontology - July 14, 2022 Category: Dentistry Source Type: research
