WDR5 promotes the tumorigenesis of oral squamous cell carcinoma via CARM1/ β-catenin axis
AbstractOral squamous cell carcinoma (OSCC) is a malignancy all over the world. WD repeat domain 5 (WDR5) is involved in cancer progression. In addition, it was reported that WDR5 is upregulated in head and neck cancer, while its role in OSCC is unknown. First, the expression of WDR5 in oral cancer tissues and cells was examined by qRT-PCR, IHF and western blot. CCK-8 assay was performed to test the cell viability. Cell migration was assessed by transwell assay. Knocking down WDR5 or CARM1 in oral cancer cells to detect its function on cancer growth, WDR5 and CARM1 were significantly upregulated in OSCC. Silencing WDR5 sup...
Source: Odontology - August 16, 2021 Category: Dentistry Source Type: research
Micro-tomographic analysis of the root trunk and pre-furcation area of the first mandibular molars
This study analyzed the root trunk (RT) and the pre-furcation area (PFA) of mandibular first molars. Thirty lower first mandibular molars extracted due to advanced periodontal disease were evaluated in a high-energy spiral computerized micro-tomography. Two gutta-percha markings on the cementoenamel junction (CEJ) and at the furcation entrance (FE) at buccal and lingual surfaces served as reference points for measurements of RT length, and PFA width and depth, at the levels of CEJ, 1 mm apical to CEJ, 2 mm apical to CEJ, and at the FE. The mean RT length was 2.49 mm at buccal and 3.18 mm at lingual sides. The mean wid...
Source: Odontology - August 13, 2021 Category: Dentistry Source Type: research
Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers
AbstractThe aim of this study was to characterize a clonal human periodontal ligament (PDL) stem cell line (line 2 –23 cells) cultured with root canal sealers based on methacrylate resin (SuperBond sealer; SB), bioactive glass (Nishika Canal Sealer BG; BG), or silicon (GuttaFlow 2; GF). The sealers were set in rubber molds to form sealer discs. Line 2–23 cells were cultured with or without the discs for 3 d ays. The cell viability was evaluated by direct cell counting and MTT assay. Inflammation-, PDL-, collagen-, and cell cycle-related gene expression was investigated by real-time RT-PCR. Collagen production was anal...
Source: Odontology - August 11, 2021 Category: Dentistry Source Type: research
Influence of intracoronal bleaching agents on the bond strength of MTA cements to composite resin and their surface morphology
The objective of the present in vitro study was to evaluate micro-tensile bond strength ( µSBS) of MTA cements to composite resin using a universal adhesive after internal bleaching procedure, and to examine surface characteristics of MTA cements exposed to bleaching agents. MTA specimens were divided into three subgroups according to the bleaching agent used which were, 37% carbamide p eroxide (CP), 35% hydrogen peroxide (HP), and no exposure as a control group (n = 12). After exposure to bleaching agent, composite resins were applied to MTA surfaces using a universal adhesive in self-etch mode. The specimens were ex...
Source: Odontology - August 9, 2021 Category: Dentistry Source Type: research
Stability of masticatory movements after placement of implant-supported denture
AbstractThe purpose of this study was to clarify the stability of masticatory movement after placement of implant-supported denture. Fourteen patients (patient group) with mandibular implants denture and maxillary complete denture and 30 dentate adults (control group) were asked to chew a boiled fishpaste, and the masticatory movement was recorded using MKG. For the 10 cycles beginning with the 5th cycle of mastication, the parameters representing the stability of masticatory movement were calculated. Data collected at 1, 3, 6, 9 months and 1, 2, 3 years after insertion of implants denture were compared between sessions...
Source: Odontology - August 7, 2021 Category: Dentistry Source Type: research
Examining the effects of acid etching duration on the bond strength between two CAD/CAM materials and one composite resin
This study aimed to evaluate the effect of three hydrofluoric acid (HF) etching periods on the micro-tensile bond strength between two CAD –CAM ceramic systems [Vita Suprinity (VS) and feldspathic CEREC blocs (CB)] and a composite resin. The ceramics were categorized into six groups based on the surface conditioning protocol used, as follows: G1: CB-HF 5% for 20 s; G2: CB-HF 5% for 40 s; G3: CB-HF 5% for 60 s; G4: VS-HF 5% for 20 s; G5: VS-HF 5% for 40 s; G6: VS-HF 5% for 60 s. Scotchbond Universal was applied onto the pretreated ceramic surfaces and covered with Filtek Z350 XT composite resin. After 24 h, the sp...
Source: Odontology - August 6, 2021 Category: Dentistry Source Type: research
The effect of diabetes mellitus on the shear bond strength of composite resin to dentin and enamel
This study aimed to determine the effect of two types of diabetes on the shear bond strength of enamel and dentin, by using the single bond universal bonding system. Sixty specimens [from 15 teeth; 5 from each group—non-diabetic (ND), Diabetic type I (D1), and Diabetic type II (D2)], were prepared with equal amounts of dentin (n = 5) and enamel (n = 5). Enamel specimens (E20) were etched with 37% phosphoric acid, for 20 s, and dentin specimens (D15) were etched for 15 s. A standard shear bond strength test was performed on all specimens. Their failure modes were also studied under a scanning electron microsco...
Source: Odontology - July 27, 2021 Category: Dentistry Source Type: research
Inter-measurement variation of masticatory performance test using gummy jelly
AbstractThe purpose of this study was to clarify the inter-measurement variation of the masticatory performance test. Forty healthy adults were divided into group A (10 males and 10 females), who did not chew the test food before experiment, and group B (10 males and 10 females) who chewed the test food before the experiment. Subjects were asked to chew a gummy jelly for 20 s on the habitual chewing side, and the amount of glucose extraction was measured. The test was repeated three times with an interval of 1 min in both groups (Group A: A1, A2, A3; Group B: B1, B2, B3). In both groups A and B, the difference between t...
Source: Odontology - July 20, 2021 Category: Dentistry Source Type: research
Influence of two carbodiimides on the bond strength of universal adhesives to dentin
AbstractThe pretreatment of dentin with cross-linking agents during bonding procedures has been proposed to improve the mechanical properties of the collagen in the hybrid layer and reduce the biodegradation of the adhesive interface. The aim of this study was to evaluate the influence of pretreatment with two carbodiimides on the dentin bond strength of universal adhesives, after thermocycling. Three universal adhesives in the self-etching mode were used (Single Bond Universal —SBU, Clearfil Universal—CLU and Ambar Universal—ABU). A self-etching adhesive system (Clearfil SE Bond—CSB) was used as control. Two carbo...
Source: Odontology - July 19, 2021 Category: Dentistry Source Type: research
Peri-implant tissue augmentation by volume-stable collagen matrix transplantation: a study of dog mandibles
AbstractThe aim of this study was to investigate histologically the amount of peri-implant tissue augmentation after volume-stable porcine collagen matrix transplantation. Six male beagle dogs were used in the experiment. P2, P4, and M1 distal roots were extracted under general anesthesia. After 6 months, implants were placed in the same sites, and volume-stable porcine collagen matrix transplantation was performed. Impressions were taken at 1 and 2 weeks and at 1, 2, and 3 months after transplantation. The dogs were euthanized at 3 months, and their mandibles were removed and scanned us ing micro-computed tomography....
Source: Odontology - July 19, 2021 Category: Dentistry Source Type: research
Vertical root fracture resistance and dentinal crack formation of root canal-treated teeth instrumented with different nickel –titanium rotary systems: an in-vitro study
AbstractTo compare the vertical root fracture (VRF) resistance of root canal-treated teeth instrumented with four different nickel –titanium (NiTi) rotary file systems and examine the dentinal crack pattern and direction using a new classification. Eighty mature mandibular premolars were selected and decoronated, leaving 13 mm of the root. The root samples were mounted in acrylic resin and divided randomly into five groups o f different NiTi file systems: Group 1—control, Group 2—T-Pro, Group 3—HyFlex CM, Group 4—TG6 and lastly Group 5—ZenFlex. Samples in Group 2 and Group 3 were instrumented up to size 25/0.0...
Source: Odontology - July 16, 2021 Category: Dentistry Source Type: research
Effect of sandblasting and/or priming treatment on the shear bond strength of self-adhesive resin cement to CAD/CAM blocks
AbstractThe aim of this study was to investigate the effect of two different priming agents and/or sandblasting on the shear bond strength of self-adhesive resin cement to the resin composite for core build-up to CAD/CAM blocks. A CAD/CAM ceramic block (GN I CERAMIC BLOCK, GC) and a CAD/CAM resin composite block (CERASMART 270, GC), a self-adhesive resin cement (G-CEM ONE, GC) and two different primers, i.e., a multipurpose primer (MP; G-Multi Primer, GC) and a ceramic primer (CP; Ceramic Primer II, GC), were examined. Five different surface treatments with priming and/or sandblasting and no surface treatment (control) wer...
Source: Odontology - July 16, 2021 Category: Dentistry Source Type: research
Prevalence of bifid and trifid mandibular canals with unusual patterns of nerve branching using cone beam computed tomography
This study reported a high prevalence (54%) of canal branching, which reinforces the importance of cone beam CT in pre-surgical planning. (Source: Odontology)
Source: Odontology - July 15, 2021 Category: Dentistry Source Type: research
Arrest of root caries with an adjuvant chlorhexidine –fluoride varnish over a 12-months observation period: a QLF-analyzed, placebo-controlled, randomized, clinical trial (RCT)
This study aimed at evaluating the effectiveness of an adjuvant chlorhexidine –fluoride varnish (Cervitec F) for prevention and arrest of root caries on elderly participants using quantitative light-induced fluorescence (QLF). 23 participants with two or three non-cavitated root carious lesions were included and assigned to three groups of different varnishes (CF: Cervitec F, P: placebo, DP: Duraphate). Agents were applied once to root surface at baseline and in follow-up after 3, 6 and 9 months. The lesions were assessed clinically and with QLF. QLF-images were analyzed regarding fluorescence loss (ΔF), lesion volume ...
Source: Odontology - July 13, 2021 Category: Dentistry Source Type: research
Clinical, radiographic and restorative parameters for short tuberosity implants placed in smokers: a retrospective study with 5 year follow-up
This study aimed to estimate and compare the clinical, radiographic, and restorative parameters around short tuberosity implants (STIs) placed in cigarette smokers (CS) and never smokers (NS). In this 60-month follow-up retrospective study, a total of 50 (37 males + 13 females) individuals who had received 82 dental implants were included. These participants were categorized into two groups as follows: (i) Group-1: 25 self-reported systemically healthy CS with 43 STIs; and (ii) Group-2: 25 self-reported systemically healthy NS with 39 STIs. In both group s, peri-implant plaque index (PI), probing depth (PD), bleeding ...
Source: Odontology - July 9, 2021 Category: Dentistry Source Type: research
