A method for screening CDV microneutralization activity in microvolume samples
CONCLUSION: This study established an economical, stable, and easy-to-operate CDV microneutralizing activity high-throughput screening method, laying a methodological foundation for the development of native CDV neutralizing antibodies based on single B cells.PMID:38195043 | DOI:10.1016/j.jviromet.2024.114883 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - January 9, 2024 Category: Virology Authors: Xiaoyu Deng Jiazi Su Bo Hu Xue Bai Source Type: research
A method for screening CDV microneutralization activity in microvolume samples
CONCLUSION: This study established an economical, stable, and easy-to-operate CDV microneutralizing activity high-throughput screening method, laying a methodological foundation for the development of native CDV neutralizing antibodies based on single B cells.PMID:38195043 | DOI:10.1016/j.jviromet.2024.114883 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - January 9, 2024 Category: Virology Authors: Xiaoyu Deng Jiazi Su Bo Hu Xue Bai Source Type: research
Quantification of low-level Cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR
CONCLUSION: dPCR can quantify low-level herpes virus DNAemia with CVs below 8%. Our results indicate that using serum-derived cell-free DNA and droplet-based dPCR is optimal for quantitating low-level viral DNAemia; however, plasma and chip-based approaches are acceptable alternatives and suitable for epidemiologic investigation.PMID:38184072 | DOI:10.1016/j.jviromet.2023.114876 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - January 6, 2024 Category: Virology Authors: DeVon N Hunter-Schlichting Rachel I Vogel Melissa A Geller Heather H Nelson Source Type: research
Quantification of low-level Cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR
CONCLUSION: dPCR can quantify low-level herpes virus DNAemia with CVs below 8%. Our results indicate that using serum-derived cell-free DNA and droplet-based dPCR is optimal for quantitating low-level viral DNAemia; however, plasma and chip-based approaches are acceptable alternatives and suitable for epidemiologic investigation.PMID:38184072 | DOI:10.1016/j.jviromet.2023.114876 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - January 6, 2024 Category: Virology Authors: DeVon N Hunter-Schlichting Rachel I Vogel Melissa A Geller Heather H Nelson Source Type: research
Quantification of low-level Cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR
CONCLUSION: dPCR can quantify low-level herpes virus DNAemia with CVs below 8%. Our results indicate that using serum-derived cell-free DNA and droplet-based dPCR is optimal for quantitating low-level viral DNAemia; however, plasma and chip-based approaches are acceptable alternatives and suitable for epidemiologic investigation.PMID:38184072 | DOI:10.1016/j.jviromet.2023.114876 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - January 6, 2024 Category: Virology Authors: DeVon N Hunter-Schlichting Rachel I Vogel Melissa A Geller Heather H Nelson Source Type: research
Identification of a potent and specific retinoic acid-inducible gene 1 pathway activator as a Hepatitis B Virus antiviral through a novel cell-based reporter assay
J Virol Methods. 2024 Jan 2:114875. doi: 10.1016/j.jviromet.2023.114875. Online ahead of print.ABSTRACTChronic Hepatitis B Virus (HBV) infection remains a global burden. To identify small molecule RIG-I agonists as antivirals against HBV, we developed an HBV-pgRNA-based interferon-β (IFN-β) luciferase reporter assay with high level of assay sensitivity, specificity and robustness. Through HTS screening, lead compound (JJ#1) was identified to activate RIG-I signaling pathway by inducing TBK1 phosphorylation. Knockdown experiments demonstrated that JJ#1-induced retinoic acid-inducible gene 1 (RIG-I) signaling pathway activ...
Source: Journal of Virological Methods - January 4, 2024 Category: Virology Authors: Liping Shi Guangyang Guo Jinying Zhou Zhanling Cheng Ren Zhu George Kukolj Chris Li Source Type: research
Identification of a potent and specific retinoic acid-inducible gene 1 pathway activator as a Hepatitis B Virus antiviral through a novel cell-based reporter assay
J Virol Methods. 2024 Jan 2:114875. doi: 10.1016/j.jviromet.2023.114875. Online ahead of print.ABSTRACTChronic Hepatitis B Virus (HBV) infection remains a global burden. To identify small molecule RIG-I agonists as antivirals against HBV, we developed an HBV-pgRNA-based interferon-β (IFN-β) luciferase reporter assay with high level of assay sensitivity, specificity and robustness. Through HTS screening, lead compound (JJ#1) was identified to activate RIG-I signaling pathway by inducing TBK1 phosphorylation. Knockdown experiments demonstrated that JJ#1-induced retinoic acid-inducible gene 1 (RIG-I) signaling pathway activ...
Source: Journal of Virological Methods - January 4, 2024 Category: Virology Authors: Liping Shi Guangyang Guo Jinying Zhou Zhanling Cheng Ren Zhu George Kukolj Chris Li Source Type: research
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research
A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research
A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research
