A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
Development of a high-throughput flow cytometric neutralization assay to screen for human enterovirus A71 (EVA71) neutralizing antibodies
This study aimed to develop a high-throughput flow cytometric neutralization assay to screen for EVA71 neutralizing antibodies, and to develop quality control materials to ensure accurate testing. The results of this study demonstrate the high potential viability of the proposed flow cytometric method. Compared to the standard method, the flow cytometric method was shown to require a smaller sample volume, provide a much faster turnaround time, provide a rapid result for interpreting the neutralizing antibody level, and allow for possible quantification of results. The observed drawbacks of the proposed method include high...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Artit Wongsa Thongkoon Priengprom Jantip Saelee Chintana Phawong Boonrat Tassaneetrithep Source Type: research
Improved Virus Concentration Methods for Wash Waters from Decontamination of Permeable and Non-Permeable Surfaces
This study adapted the dead-end hollow-fiber ultrafiltration (D-HFUF) system to wash waters, including a separate procedure for recovering particle attached viruses. Simulated wash water was created using dechlorinated tap water containing a mild surfactant (0.05% Tween 80). To determine virus recovery efficiencies, measured amounts of somatic and F+ coliphage were spiked into 2-liter volumes of wash water under the following scenarios: 1) wash water was amended with a measured amount of sterile river sediment with no sediment separation prior to filter concentration; or 2) sediment added to wash water was allowed to settl...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Brittany N Hurst Asja Korajkic Adin Pemberton Brian R McMinn Source Type: research
A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses
J Virol Methods. 2023 Sep 29:114824. doi: 10.1016/j.jviromet.2023.114824. Online ahead of print.ABSTRACTPrimary infection or reactivation of latent human cytomegalovirus (HCMV) or herpes simplex viruses (HSV) 1 or 2 during pregnancy can transmit the virus in utero or during natural childbirth to the fetus. The majority of these infections are asymptomatic at birth but may present later with potentially lethal disseminated infection or meningitis (HSV), or long-term neurodevelopmental sequelae including sensorineural hearing loss or neurodevelopmental impairments (HCMV). Unfortunately, early signs and symptoms of disseminat...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Anshu Gupta Shelley M Lawrence Stephanie I Fraley Source Type: research
Comparative study between virus neutralisation testing and other serological methods detecting anti-SARS-CoV-2 antibodies in Europe, 2021
J Virol Methods. 2023 Sep 29:114825. doi: 10.1016/j.jviromet.2023.114825. Online ahead of print.ABSTRACTOne consequence of the ongoing coronavirus disease pandemic was the rapid development of both in-house and commercial serological assays detecting anti-SARS-CoV-2 antibodies, in an effort to reliably detect acute and past SARS-CoV-2 infections. It is crucial to evaluate the quality of these serological tests and consequently the sero-epidemiological studies that are performed with the respective tests. Here, we describe the set-up and results of a comparative study, in which a laboratory contracted by the European Centre...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Ramona M ögling Johan Reimerink Kamelia R Stanoeva Maria Keramarou Raquel Guiomar In ês Costa Anu Haveri Barbara Holzer G ülay Korukluoğlu Trung Nguyen Gatis Pakarna Katarzyna Pancer Katarina Prosenc Trilar Jelena Protic Marijana Stojanovi ć Riccardo Source Type: research
Evaluation of Illumina ® COVIDSeq™ as a Tool for Omicron SARS-CoV-2 Characterisation
This study demonstrates the effectiveness and versatility of the amplicon-based NGS characterisation method for SARS-CoV-2, providing a foundation for further research and development of custom-designed amplicon panels targeting different microorganisms.PMID:37778540 | DOI:10.1016/j.jviromet.2023.114827 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Kym Lowry Michelle J Bauer Cameron Buckley Claire Wang Amanda Bordin Steven Badman Patrick N A Harris Ian Mackay David Whiley Source Type: research
Development of a high-throughput flow cytometric neutralization assay to screen for human enterovirus A71 (EVA71) neutralizing antibodies
This study aimed to develop a high-throughput flow cytometric neutralization assay to screen for EVA71 neutralizing antibodies, and to develop quality control materials to ensure accurate testing. The results of this study demonstrate the high potential viability of the proposed flow cytometric method. Compared to the standard method, the flow cytometric method was shown to require a smaller sample volume, provide a much faster turnaround time, provide a rapid result for interpreting the neutralizing antibody level, and allow for possible quantification of results. The observed drawbacks of the proposed method include high...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Artit Wongsa Thongkoon Priengprom Jantip Saelee Chintana Phawong Boonrat Tassaneetrithep Source Type: research
Improved Virus Concentration Methods for Wash Waters from Decontamination of Permeable and Non-Permeable Surfaces
This study adapted the dead-end hollow-fiber ultrafiltration (D-HFUF) system to wash waters, including a separate procedure for recovering particle attached viruses. Simulated wash water was created using dechlorinated tap water containing a mild surfactant (0.05% Tween 80). To determine virus recovery efficiencies, measured amounts of somatic and F+ coliphage were spiked into 2-liter volumes of wash water under the following scenarios: 1) wash water was amended with a measured amount of sterile river sediment with no sediment separation prior to filter concentration; or 2) sediment added to wash water was allowed to settl...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Brittany N Hurst Asja Korajkic Adin Pemberton Brian R McMinn Source Type: research
A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses
J Virol Methods. 2023 Sep 29:114824. doi: 10.1016/j.jviromet.2023.114824. Online ahead of print.ABSTRACTPrimary infection or reactivation of latent human cytomegalovirus (HCMV) or herpes simplex viruses (HSV) 1 or 2 during pregnancy can transmit the virus in utero or during natural childbirth to the fetus. The majority of these infections are asymptomatic at birth but may present later with potentially lethal disseminated infection or meningitis (HSV), or long-term neurodevelopmental sequelae including sensorineural hearing loss or neurodevelopmental impairments (HCMV). Unfortunately, early signs and symptoms of disseminat...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Anshu Gupta Shelley M Lawrence Stephanie I Fraley Source Type: research
Comparative study between virus neutralisation testing and other serological methods detecting anti-SARS-CoV-2 antibodies in Europe, 2021
J Virol Methods. 2023 Sep 29:114825. doi: 10.1016/j.jviromet.2023.114825. Online ahead of print.ABSTRACTOne consequence of the ongoing coronavirus disease pandemic was the rapid development of both in-house and commercial serological assays detecting anti-SARS-CoV-2 antibodies, in an effort to reliably detect acute and past SARS-CoV-2 infections. It is crucial to evaluate the quality of these serological tests and consequently the sero-epidemiological studies that are performed with the respective tests. Here, we describe the set-up and results of a comparative study, in which a laboratory contracted by the European Centre...
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Ramona M ögling Johan Reimerink Kamelia R Stanoeva Maria Keramarou Raquel Guiomar In ês Costa Anu Haveri Barbara Holzer G ülay Korukluoğlu Trung Nguyen Gatis Pakarna Katarzyna Pancer Katarina Prosenc Trilar Jelena Protic Marijana Stojanovi ć Riccardo Source Type: research
Evaluation of Illumina ® COVIDSeq™ as a Tool for Omicron SARS-CoV-2 Characterisation
This study demonstrates the effectiveness and versatility of the amplicon-based NGS characterisation method for SARS-CoV-2, providing a foundation for further research and development of custom-designed amplicon panels targeting different microorganisms.PMID:37778540 | DOI:10.1016/j.jviromet.2023.114827 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Kym Lowry Michelle J Bauer Cameron Buckley Claire Wang Amanda Bordin Steven Badman Patrick N A Harris Ian Mackay David Whiley Source Type: research
Establishment of a p30-based lateral flow assay for African swine fever virus detection
In this study, we developed a lateral flow assay to detect ASFV on-site. The VDRG® ASFV Ag Rapid Kit was established using two monoclonal antibodies (mAbs) against the p30 protein. The conjunction pad of the kit was coated with a mixture of the mAb and colloidal gold. This rapid kit was capable of detecting 11.5 ng of antigen and 0.16 HAD50 of virus from samples, in 20 min for the entire procedure. It passed cross-specific tests using common viruses that cause infectious diseases in pigs. ASFV was detected after 4 days in experimental infection in pigs by the kit. The specificity and sensitivity of the kit for clinical sa...
Source: Journal of Virological Methods - September 25, 2023 Category: Virology Authors: Thi Thu Hang Vu Van Phan Le Dae Gwin Jeong Minjoo Yeom Jinsik Oh BoKyu Kang Song-Kyu Park Daesub Song Source Type: research
