Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples
In conclusion, due to its simplicity, rapidness, sensitivity, and specificity, RT-LAMP has tremendous potential as a point-of-care tool; nevertheless, more research is needed to utilize it for detecting SARS-CoV-2, particularly in direct swab samples.PMID:38103738 | DOI:10.1016/j.jviromet.2023.114871 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 16, 2023 Category: Virology Authors: Ramin Pourakbari Mohammad Gholami Ali Shakerimoghaddam Farhad Motavalli Khiavi Mojgan Mohammadimehr Mehdi Shakouri Khomartash Source Type: research
Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples
In conclusion, due to its simplicity, rapidness, sensitivity, and specificity, RT-LAMP has tremendous potential as a point-of-care tool; nevertheless, more research is needed to utilize it for detecting SARS-CoV-2, particularly in direct swab samples.PMID:38103738 | DOI:10.1016/j.jviromet.2023.114871 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 16, 2023 Category: Virology Authors: Ramin Pourakbari Mohammad Gholami Ali Shakerimoghaddam Farhad Motavalli Khiavi Mojgan Mohammadimehr Mehdi Shakouri Khomartash Source Type: research
Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples
In conclusion, due to its simplicity, rapidness, sensitivity, and specificity, RT-LAMP has tremendous potential as a point-of-care tool; nevertheless, more research is needed to utilize it for detecting SARS-CoV-2, particularly in direct swab samples.PMID:38103738 | DOI:10.1016/j.jviromet.2023.114871 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 16, 2023 Category: Virology Authors: Ramin Pourakbari Mohammad Gholami Ali Shakerimoghaddam Farhad Motavalli Khiavi Mojgan Mohammadimehr Mehdi Shakouri Khomartash Source Type: research
Development of an accurate and rapid method for whole genome characterization of canine parvovirus
J Virol Methods. 2023 Dec 10:114870. doi: 10.1016/j.jviromet.2023.114870. Online ahead of print.ABSTRACTCanine parvovirus is a highly contagious pathogen affecting domestic dogs and other carnivores globally. Monitoring CPV through continuous genomic surveillance is crucial for mapping variability and developing effective control measures. Here, we developed a method using multiplex-PCR-next-generation sequencing to obtain full-length CPV genomes directly from clinical samples. This approach utilizes tiling and tailed amplicons to amplify overlapping fragments of roughly 250 base pairs. This enables the creation of Illumin...
Source: Journal of Virological Methods - December 12, 2023 Category: Virology Authors: Emma Condon Sof ía Grecco Ana Marandino Jaime Aldaz Javier Enciso Luis Alfaro Danilo Bucafusco Ruben P érez Yanina Panzera Source Type: research
Development of an accurate and rapid method for whole genome characterization of canine parvovirus
J Virol Methods. 2023 Dec 10:114870. doi: 10.1016/j.jviromet.2023.114870. Online ahead of print.ABSTRACTCanine parvovirus is a highly contagious pathogen affecting domestic dogs and other carnivores globally. Monitoring CPV through continuous genomic surveillance is crucial for mapping variability and developing effective control measures. Here, we developed a method using multiplex-PCR-next-generation sequencing to obtain full-length CPV genomes directly from clinical samples. This approach utilizes tiling and tailed amplicons to amplify overlapping fragments of roughly 250 base pairs. This enables the creation of Illumin...
Source: Journal of Virological Methods - December 12, 2023 Category: Virology Authors: Emma Condon Sof ía Grecco Ana Marandino Jaime Aldaz Javier Enciso Luis Alfaro Danilo Bucafusco Ruben P érez Yanina Panzera Source Type: research
Development of an accurate and rapid method for whole genome characterization of canine parvovirus
J Virol Methods. 2023 Dec 10:114870. doi: 10.1016/j.jviromet.2023.114870. Online ahead of print.ABSTRACTCanine parvovirus is a highly contagious pathogen affecting domestic dogs and other carnivores globally. Monitoring CPV through continuous genomic surveillance is crucial for mapping variability and developing effective control measures. Here, we developed a method using multiplex-PCR-next-generation sequencing to obtain full-length CPV genomes directly from clinical samples. This approach utilizes tiling and tailed amplicons to amplify overlapping fragments of roughly 250 base pairs. This enables the creation of Illumin...
Source: Journal of Virological Methods - December 12, 2023 Category: Virology Authors: Emma Condon Sof ía Grecco Ana Marandino Jaime Aldaz Javier Enciso Luis Alfaro Danilo Bucafusco Ruben P érez Yanina Panzera Source Type: research
Development of an accurate and rapid method for whole genome characterization of canine parvovirus
J Virol Methods. 2023 Dec 10:114870. doi: 10.1016/j.jviromet.2023.114870. Online ahead of print.ABSTRACTCanine parvovirus is a highly contagious pathogen affecting domestic dogs and other carnivores globally. Monitoring CPV through continuous genomic surveillance is crucial for mapping variability and developing effective control measures. Here, we developed a method using multiplex-PCR-next-generation sequencing to obtain full-length CPV genomes directly from clinical samples. This approach utilizes tiling and tailed amplicons to amplify overlapping fragments of roughly 250 base pairs. This enables the creation of Illumin...
Source: Journal of Virological Methods - December 12, 2023 Category: Virology Authors: Emma Condon Sof ía Grecco Ana Marandino Jaime Aldaz Javier Enciso Luis Alfaro Danilo Bucafusco Ruben P érez Yanina Panzera Source Type: research
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome
In this study, performance characteristics such as the quantification range, sensitivity, precision, and specificity of the dPCR technique was compared to qPCR technique for the detection and quantification of IBV genome loads in serial dilutions of IBV positive plasmid DNA, and IBV infected chicken tissue and swab samples. The quantification range of the qPCR assay was wider than that of the dPCR assay, however dPCR had a higher sensitivity compared to qPCR. The precision of quantification of DNA in plasmid samples in terms of repeatability and reproducibility of results was higher when using the dPCR assay compared to qP...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Ishara M Isham Shahnas M Najimudeen Susan C Cork Ashish Gupta Mohamed Faizal Abdul-Careem Source Type: research
Assessing the Efficacy of Different Bead-Based Assays in Capturing Hepatitis E Virus
J Virol Methods. 2023 Dec 5:114860. doi: 10.1016/j.jviromet.2023.114860. Online ahead of print.ABSTRACTHepatitis E virus (HEV) generally causes acute liver infection in humans and its transmission could be waterborne, foodborne, bloodborne, or zoonotic. To date, there is no standard method for the detection of HEV from food and environmental samples. Herein, we explored the possibility of using magnetic beads for the capture and detection of HEV. For this purpose, we employed Dynabeads M-270 Epoxy magnetic beads, coated with different monoclonal antibodies (mAbs) against HEV capsid protein, and the Nanotrap Microbiome A Pa...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Jeremy Tan Jennifer Harlow Jonathon Cecillon Neda Nasheri Source Type: research
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome
In this study, performance characteristics such as the quantification range, sensitivity, precision, and specificity of the dPCR technique was compared to qPCR technique for the detection and quantification of IBV genome loads in serial dilutions of IBV positive plasmid DNA, and IBV infected chicken tissue and swab samples. The quantification range of the qPCR assay was wider than that of the dPCR assay, however dPCR had a higher sensitivity compared to qPCR. The precision of quantification of DNA in plasmid samples in terms of repeatability and reproducibility of results was higher when using the dPCR assay compared to qP...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Ishara M Isham Shahnas M Najimudeen Susan C Cork Ashish Gupta Mohamed Faizal Abdul-Careem Source Type: research
Assessing the Efficacy of Different Bead-Based Assays in Capturing Hepatitis E Virus
J Virol Methods. 2023 Dec 5:114860. doi: 10.1016/j.jviromet.2023.114860. Online ahead of print.ABSTRACTHepatitis E virus (HEV) generally causes acute liver infection in humans and its transmission could be waterborne, foodborne, bloodborne, or zoonotic. To date, there is no standard method for the detection of HEV from food and environmental samples. Herein, we explored the possibility of using magnetic beads for the capture and detection of HEV. For this purpose, we employed Dynabeads M-270 Epoxy magnetic beads, coated with different monoclonal antibodies (mAbs) against HEV capsid protein, and the Nanotrap Microbiome A Pa...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Jeremy Tan Jennifer Harlow Jonathon Cecillon Neda Nasheri Source Type: research
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome
In this study, performance characteristics such as the quantification range, sensitivity, precision, and specificity of the dPCR technique was compared to qPCR technique for the detection and quantification of IBV genome loads in serial dilutions of IBV positive plasmid DNA, and IBV infected chicken tissue and swab samples. The quantification range of the qPCR assay was wider than that of the dPCR assay, however dPCR had a higher sensitivity compared to qPCR. The precision of quantification of DNA in plasmid samples in terms of repeatability and reproducibility of results was higher when using the dPCR assay compared to qP...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Ishara M Isham Shahnas M Najimudeen Susan C Cork Ashish Gupta Mohamed Faizal Abdul-Careem Source Type: research
Assessing the Efficacy of Different Bead-Based Assays in Capturing Hepatitis E Virus
J Virol Methods. 2023 Dec 5:114860. doi: 10.1016/j.jviromet.2023.114860. Online ahead of print.ABSTRACTHepatitis E virus (HEV) generally causes acute liver infection in humans and its transmission could be waterborne, foodborne, bloodborne, or zoonotic. To date, there is no standard method for the detection of HEV from food and environmental samples. Herein, we explored the possibility of using magnetic beads for the capture and detection of HEV. For this purpose, we employed Dynabeads M-270 Epoxy magnetic beads, coated with different monoclonal antibodies (mAbs) against HEV capsid protein, and the Nanotrap Microbiome A Pa...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Jeremy Tan Jennifer Harlow Jonathon Cecillon Neda Nasheri Source Type: research
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome
In this study, performance characteristics such as the quantification range, sensitivity, precision, and specificity of the dPCR technique was compared to qPCR technique for the detection and quantification of IBV genome loads in serial dilutions of IBV positive plasmid DNA, and IBV infected chicken tissue and swab samples. The quantification range of the qPCR assay was wider than that of the dPCR assay, however dPCR had a higher sensitivity compared to qPCR. The precision of quantification of DNA in plasmid samples in terms of repeatability and reproducibility of results was higher when using the dPCR assay compared to qP...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Ishara M Isham Shahnas M Najimudeen Susan C Cork Ashish Gupta Mohamed Faizal Abdul-Careem Source Type: research
Assessing the Efficacy of Different Bead-Based Assays in Capturing Hepatitis E Virus
J Virol Methods. 2023 Dec 5:114860. doi: 10.1016/j.jviromet.2023.114860. Online ahead of print.ABSTRACTHepatitis E virus (HEV) generally causes acute liver infection in humans and its transmission could be waterborne, foodborne, bloodborne, or zoonotic. To date, there is no standard method for the detection of HEV from food and environmental samples. Herein, we explored the possibility of using magnetic beads for the capture and detection of HEV. For this purpose, we employed Dynabeads M-270 Epoxy magnetic beads, coated with different monoclonal antibodies (mAbs) against HEV capsid protein, and the Nanotrap Microbiome A Pa...
Source: Journal of Virological Methods - December 7, 2023 Category: Virology Authors: Jeremy Tan Jennifer Harlow Jonathon Cecillon Neda Nasheri Source Type: research
