Improved Virus Concentration Methods for Wash Waters from Decontamination of Permeable and Non-Permeable Surfaces

This study adapted the dead-end hollow-fiber ultrafiltration (D-HFUF) system to wash waters, including a separate procedure for recovering particle attached viruses. Simulated wash water was created using dechlorinated tap water containing a mild surfactant (0.05% Tween 80). To determine virus recovery efficiencies, measured amounts of somatic and F+ coliphage were spiked into 2-liter volumes of wash water under the following scenarios: 1) wash water was amended with a measured amount of sterile river sediment with no sediment separation prior to filter concentration; or 2) sediment added to wash water was allowed to settle prior to filter concentrating clarified liquid portions, while precipitated sediment was subjected to viral extraction techniques to recover particle attached virus; and 3) the optimized method was deployed on non-porous and porous surfaces to simulate a decontamination clean-up event. Separation of sediment prior to D-HFUF significantly increased recovery of coliphages, (P=<0.0001) versus filtration of sediment and liquids simultaneously. A tryptic soy broth (TSB) elution solution was significantly more effective (P=≤0.010) for recovery of both somatic and F+ coliphage, (108±9% and 92±9%, respectively), compared to elution buffers containing various surfactants (sodium hexametaphosphate, Tween 80) for recovering particle attached virus. Simulating a biocontaminate clean-up event (using the optimized sediment separation and elution protocol) resulte...

Source: Journal of Virological Methods - October 1, 2023 Category: Virology Authors: Brittany N Hurst Asja Korajkic Adin Pemberton Brian R McMinn Source Type: research