Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus
J Virol Methods. 2024 Mar 12;326:114916. doi: 10.1016/j.jviromet.2024.114916. Online ahead of print.ABSTRACTLumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection. Using western blot, indirect immune-fluorescence assay, flow cytometry, and transmission electron microscopy methods, this study is the first to identify the murine osteoblastic cell line MC3T3-E...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Ting You Meng Wang Hongqiang Zhang Xiangwei Wang Xiaolong Gao Xiangping Yin Yuefeng Sun Guirong Wang Hao-Tai Chen Shanhui Ren Source Type: research
Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: A study in swine
J Virol Methods. 2024 Mar 12;326:114915. doi: 10.1016/j.jviromet.2024.114915. Online ahead of print.ABSTRACTCrimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability of a multi-species ELISA has led to an increase in recent serosurveillance studies as well as in the range of species reported to be exposed to CCHFV in the field, including wild boar (Sus scrofa). However, development and validatio...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Caroline Bost Sabrina Castro-Scholten Balal Sadeghi David Cano-Terriza Mario Fr ías Sa úl Jiménez-Ruiz Martin H Groschup Ignacio Garc ía-Bocanegra Kerstin Fischer Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus
J Virol Methods. 2024 Mar 12;326:114916. doi: 10.1016/j.jviromet.2024.114916. Online ahead of print.ABSTRACTLumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection. Using western blot, indirect immune-fluorescence assay, flow cytometry, and transmission electron microscopy methods, this study is the first to identify the murine osteoblastic cell line MC3T3-E...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Ting You Meng Wang Hongqiang Zhang Xiangwei Wang Xiaolong Gao Xiangping Yin Yuefeng Sun Guirong Wang Hao-Tai Chen Shanhui Ren Source Type: research
Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: a study in swine
J Virol Methods. 2024 Mar 11:114915. doi: 10.1016/j.jviromet.2024.114915. Online ahead of print.ABSTRACTCrimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability of a multi-species ELISA has led to an increase in recent serosurveillance studies as well as in the range of species reported to be exposed to CCHFV in the field, including wild boar (Sus scrofa). However, development and validation of...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Caroline Bost Sabrina Castro-Scholten Balal Sadeghi David Cano-Terriza Mario Fr ías Sa úl Jiménez-Ruiz Martin H Groschup Ignacio Garc ía-Bocanegra Kerstin Fischer Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to Lumpy skin disease virus
J Virol Methods. 2024 Mar 11:114916. doi: 10.1016/j.jviromet.2024.114916. Online ahead of print.ABSTRACTLumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection. Using western blot, indirect immune-fluorescence assay, flow cytometry, and transmission electron microscopy methods, this study is the first to identify the murine osteoblastic cell line MC3T3-E1 as...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Ting You Meng Wang Hongqiang Zhang Xiangwei Wang Xiaolong Gao Xiangping Yin Yuefeng Sun Guirong Wang Hao-Tai Chen Shanhui Ren Source Type: research
Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: a study in swine
J Virol Methods. 2024 Mar 11:114915. doi: 10.1016/j.jviromet.2024.114915. Online ahead of print.ABSTRACTCrimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability of a multi-species ELISA has led to an increase in recent serosurveillance studies as well as in the range of species reported to be exposed to CCHFV in the field, including wild boar (Sus scrofa). However, development and validation of...
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Caroline Bost Sabrina Castro-Scholten Balal Sadeghi David Cano-Terriza Mario Fr ías Sa úl Jiménez-Ruiz Martin H Groschup Ignacio Garc ía-Bocanegra Kerstin Fischer Source Type: research
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
J Virol Methods. 2024 Mar 6;326:114914. doi: 10.1016/j.jviromet.2024.114914. Online ahead of print.ABSTRACTPolioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family Picornaviridae. As we approach polio eradication, accurate and timely detection of poliovirus in stool from AFP cases becomes vital to success for the eradication efforts. Direct detection of PV from clinical diagnostic samples using nucleic acid (NA) extraction and real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead of the current standard method of virus i...
Source: Journal of Virological Methods - March 8, 2024 Category: Virology Authors: Stacey Jeffries Miles Chelsea Harrington Hong Sun Ashley Deas M Steven Oberste W Allan Nix Everardo Vega Nancy Gerloff Source Type: research
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
J Virol Methods. 2024 Mar 6;326:114914. doi: 10.1016/j.jviromet.2024.114914. Online ahead of print.ABSTRACTPolioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family Picornaviridae. As we approach polio eradication, accurate and timely detection of poliovirus in stool from AFP cases becomes vital to success for the eradication efforts. Direct detection of PV from clinical diagnostic samples using nucleic acid (NA) extraction and real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead of the current standard method of virus i...
Source: Journal of Virological Methods - March 8, 2024 Category: Virology Authors: Stacey Jeffries Miles Chelsea Harrington Hong Sun Ashley Deas M Steven Oberste W Allan Nix Everardo Vega Nancy Gerloff Source Type: research
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
J Virol Methods. 2024 Mar 6:114914. doi: 10.1016/j.jviromet.2024.114914. Online ahead of print.ABSTRACTPolioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family Picornaviridae. As we approach polio eradication, accurate and timely detection of poliovirus in stool from AFP cases becomes vital to success for the eradication efforts. Direct detection of PV from clinical diagnostic samples using nucleic acid (NA) extraction and real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead of the current standard method of virus isola...
Source: Journal of Virological Methods - March 8, 2024 Category: Virology Authors: Stacey Jeffries Miles Chelsea Harrington Hong Sun Ashley Deas M Steve Oberste W Allan Nix Everardo Vega Nancy Gerloff Source Type: research
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication
J Virol Methods. 2024 Mar 6:114914. doi: 10.1016/j.jviromet.2024.114914. Online ahead of print.ABSTRACTPolioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family Picornaviridae. As we approach polio eradication, accurate and timely detection of poliovirus in stool from AFP cases becomes vital to success for the eradication efforts. Direct detection of PV from clinical diagnostic samples using nucleic acid (NA) extraction and real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead of the current standard method of virus isola...
Source: Journal of Virological Methods - March 8, 2024 Category: Virology Authors: Stacey Jeffries Miles Chelsea Harrington Hong Sun Ashley Deas M Steve Oberste W Allan Nix Everardo Vega Nancy Gerloff Source Type: research
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region
J Virol Methods. 2024 Mar 5:114913. doi: 10.1016/j.jviromet.2024.114913. Online ahead of print.ABSTRACTHepatitis B virus (HBV) infection is a global public health burden and affects approximatively 300 million people around the world. Since, HBV population is represented with genetic diversity, having different viral effects. Development of a new prognosis method play a key role on the efficiency of the different treatment. The HBx protein of HBV has a potential role in Hepatocellular Carcinoma (HCC), which makes it a valuable target for HCC prognosis. In this context, the first quantitative real-time PCR (qRT-PCR) assay i...
Source: Journal of Virological Methods - March 7, 2024 Category: Virology Authors: Salma Madihi Chaimaa Laassili Samia Boukaira Warda Baha Meriem Khyatti Abdelmajid Zyad Sanae Ben Mkaddem Abdelouaheb Benani Source Type: research
Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in saliva samples
This study aimed to evaluate diagnostic accuracy of SARS-CoV-2 RNA detection in saliva samples treated with a guanidine-based or guanidine-free inactivator, using nasopharyngeal swab samples (NPS) as referents. Based on the NPS reverse transcription-polymerase chain reaction (RT-PCR) results, participants were classified as with or without COVID-19. Fifty sets of samples comprising NPS, self-collected raw saliva, and saliva with a guanidine-based, and guanidine-free inactivator were collected from each group. In patients with COVID-19, the sensitivity of direct RT-PCR using raw saliva and saliva treated with a guanidine-ba...
Source: Journal of Virological Methods - March 7, 2024 Category: Virology Authors: Takashi Katsuno Moto Kimura Junko Terada-Hirashima Yukumasa Kazuyama Masato Ikeda Ataru Moriya Masami Kurokawa Ayano Motohashi Erina Isaka Momoko Morishita Kazuki Kawajiri Kazuo Hakkaku Susumu Saito Yuriko Terayama Yuriko Sugiura Yoh Yamaguchi Hiroshi Tak Source Type: research
Specific molecular peak analysis by ion mobility spectrometry of volatile organic compounds in urine of COVID-19 patients: A novel diagnostic approach
This study investigated a novel diagnostic approach to Covid-19 measuring volatile organic compounds (VOC) from patients' urine.METHODS: Between June 2020 and February 2021, 84 patients with positive RT-PCR for SARS-CoV-2 were recruited as well as 54 symptomatic individuals with negative RT-PCR. Midstream urine samples were obtained for VOC analysis using ion mobility spectrometry (IMS) which detects individual molecular components of a gas sample based on their size, configuration, and charge after ionization.RESULTS: Peak analysis of the 84 Covid and 54 control samples showed good group separation. In total, 37 individua...
Source: Journal of Virological Methods - March 7, 2024 Category: Virology Authors: T Boeselt P Terhorst J Kroenig C Nell M Spielmanns U Boas M Veith C Vogelmeier T Greulich A R Koczulla B Beutel J Huber H Heers Source Type: research
