Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research

Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research

Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research

Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research

Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research

Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV
CONCLUSIONS: In this study, a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established for the detection of VZV. The assay has good sensitivity, specificity and repeatability, which provides strong technical support and product guarantee for the rapid clinical detection of VZV.PMID:38154579 | DOI:10.1016/j.jviromet.2023.114874 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 28, 2023 Category: Virology Authors: Aiping Wang Na Liu Jianguo Zhao Yan Niu Yumei Chen Jingming Zhou Enping Liu Gaiping Zhang Source Type: research

A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research

A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research

A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research

A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China
In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2~8.9% and 2.6~8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiologi...
Source: Journal of Virological Methods - December 24, 2023 Category: Virology Authors: Dalin Hong Jinni Bian Lingyou Zeng Shiting Huang Yifeng Qin Ying Chen Zuzhang Wei Weijian Huang Kang Ouyang Source Type: research

m-PIMA ™ HIV1/2 VL: a suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa
This study evaluated the performance of the m-PIMA™ HIV1/2 Viral Load for the quantification of both HIV-1 and HIV-2 RNA viral load. A total of 555 HIV-1 and 90 HIV-2 samples previously tested by Abbott RealTime HIV-1 (Abbott, Chicago, USA) and Generic HIV-2® Charge virale (Biocentric, France) were tested using the m-PIMA™ HIV1/2 Viral Load at the HIV National Reference lab in Senegal. For HIV-1, Pearson correlation and Bland-Altman plots showed a coefficient r=0.97 and a bias of -0.11 log10 copies/ml (95% confidence interval [CI]: -0.086 to -0.133 log10 copies/ml) for the m-PIMA™ HIV1/2 Viral Load, respectively. Se...
Source: Journal of Virological Methods - December 21, 2023 Category: Virology Authors: Halimatou Diop-Ndiaye Pauline Yacine S ène Khadidiatou Coulibaly Mari ème Diallo Sada Diallo Karim Diop Aissatou Sow-Ndoye Mengue Fall Anna Julienne Selbe Ndiaye Evans Mathebula Adjratou Aissatou Ba Charlotte Lejeune Ndeye Marie Pascaline Manga Makhtar Source Type: research

m-PIMA ™ HIV1/2 VL: a suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa
This study evaluated the performance of the m-PIMA™ HIV1/2 Viral Load for the quantification of both HIV-1 and HIV-2 RNA viral load. A total of 555 HIV-1 and 90 HIV-2 samples previously tested by Abbott RealTime HIV-1 (Abbott, Chicago, USA) and Generic HIV-2® Charge virale (Biocentric, France) were tested using the m-PIMA™ HIV1/2 Viral Load at the HIV National Reference lab in Senegal. For HIV-1, Pearson correlation and Bland-Altman plots showed a coefficient r=0.97 and a bias of -0.11 log10 copies/ml (95% confidence interval [CI]: -0.086 to -0.133 log10 copies/ml) for the m-PIMA™ HIV1/2 Viral Load, respectively. Se...
Source: Journal of Virological Methods - December 21, 2023 Category: Virology Authors: Halimatou Diop-Ndiaye Pauline Yacine S ène Khadidiatou Coulibaly Mari ème Diallo Sada Diallo Karim Diop Aissatou Sow-Ndoye Mengue Fall Anna Julienne Selbe Ndiaye Evans Mathebula Adjratou Aissatou Ba Charlotte Lejeune Ndeye Marie Pascaline Manga Makhtar Source Type: research

m-PIMA ™ HIV1/2 VL: a suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa
This study evaluated the performance of the m-PIMA™ HIV1/2 Viral Load for the quantification of both HIV-1 and HIV-2 RNA viral load. A total of 555 HIV-1 and 90 HIV-2 samples previously tested by Abbott RealTime HIV-1 (Abbott, Chicago, USA) and Generic HIV-2® Charge virale (Biocentric, France) were tested using the m-PIMA™ HIV1/2 Viral Load at the HIV National Reference lab in Senegal. For HIV-1, Pearson correlation and Bland-Altman plots showed a coefficient r=0.97 and a bias of -0.11 log10 copies/ml (95% confidence interval [CI]: -0.086 to -0.133 log10 copies/ml) for the m-PIMA™ HIV1/2 Viral Load, respectively. Se...
Source: Journal of Virological Methods - December 21, 2023 Category: Virology Authors: Halimatou Diop-Ndiaye Pauline Yacine S ène Khadidiatou Coulibaly Mari ème Diallo Sada Diallo Karim Diop Aissatou Sow-Ndoye Mengue Fall Anna Julienne Selbe Ndiaye Evans Mathebula Adjratou Aissatou Ba Charlotte Lejeune Ndeye Marie Pascaline Manga Makhtar Source Type: research

Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples
In conclusion, due to its simplicity, rapidness, sensitivity, and specificity, RT-LAMP has tremendous potential as a point-of-care tool; nevertheless, more research is needed to utilize it for detecting SARS-CoV-2, particularly in direct swab samples.PMID:38103738 | DOI:10.1016/j.jviromet.2023.114871 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 16, 2023 Category: Virology Authors: Ramin Pourakbari Mohammad Gholami Ali Shakerimoghaddam Farhad Motavalli Khiavi Mojgan Mohammadimehr Mehdi Shakouri Khomartash Source Type: research

Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples
In conclusion, due to its simplicity, rapidness, sensitivity, and specificity, RT-LAMP has tremendous potential as a point-of-care tool; nevertheless, more research is needed to utilize it for detecting SARS-CoV-2, particularly in direct swab samples.PMID:38103738 | DOI:10.1016/j.jviromet.2023.114871 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - December 16, 2023 Category: Virology Authors: Ramin Pourakbari Mohammad Gholami Ali Shakerimoghaddam Farhad Motavalli Khiavi Mojgan Mohammadimehr Mehdi Shakouri Khomartash Source Type: research