Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India
In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of the PCR amplicons on agarose gel. Their diagnostic performance relative to the mPCR assay in use in India was evaluated on 169 clinical samples and 210 cell culture grown virus isolates. The relative diagnostic sensitivity was found to be 99.69%, 98.78% and 99.08% for primer combinations 1, 2 and 3, respectively. These assays proved their worth by detecting s...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Jajati Keshari Mohapatra Manoranjan Rout Saravanan Subramaniam Priyabrata Giri Shyam Singh Dahiya Sagar Sangam Rautaray Jitendra Kumar Biswal Nihar Ranjan Sahoo Rabindra Prasad Singh Source Type: research
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage
J Virol Methods. 2023 Sep 30:114830. doi: 10.1016/j.jviromet.2023.114830. Online ahead of print.ABSTRACTTurkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study we report their functionality after storage for 18 months, at -80 °C. Three influenza A subtypes, namely, H3N2, H1N1 and H5N1, were used in the study. Hemagglutination assay was p...
Source: Journal of Virological Methods - October 2, 2023 Category: Virology Authors: Shailesh D Pawar Deeksha S Tare Sadhana S Kode Sachin S Keng Source Type: research
