Detection of HIV-1 matrix protein p17 in sera of viremic or aviremic patients
J Virol Methods. 2023 Nov 27:114858. doi: 10.1016/j.jviromet.2023.114858. Online ahead of print.ABSTRACTPeople living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the development of these HIV-1-associated conditions. The HIV-1 matrix protein p17 (p17) is released and accumulates in different...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Alberto Zani Serena Messali Matteo Uggeri Carlo Bonfanti Arnaldo Caruso Francesca Caccuri Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Detection of HIV-1 matrix protein p17 in sera of viremic or aviremic patients
J Virol Methods. 2023 Nov 27:114858. doi: 10.1016/j.jviromet.2023.114858. Online ahead of print.ABSTRACTPeople living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the development of these HIV-1-associated conditions. The HIV-1 matrix protein p17 (p17) is released and accumulates in different...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Alberto Zani Serena Messali Matteo Uggeri Carlo Bonfanti Arnaldo Caruso Francesca Caccuri Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Detection of HIV-1 matrix protein p17 in sera of viremic or aviremic patients
J Virol Methods. 2023 Nov 27:114858. doi: 10.1016/j.jviromet.2023.114858. Online ahead of print.ABSTRACTPeople living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the development of these HIV-1-associated conditions. The HIV-1 matrix protein p17 (p17) is released and accumulates in different...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Alberto Zani Serena Messali Matteo Uggeri Carlo Bonfanti Arnaldo Caruso Francesca Caccuri Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Detection of HIV-1 matrix protein p17 in sera of viremic or aviremic patients
J Virol Methods. 2023 Nov 27:114858. doi: 10.1016/j.jviromet.2023.114858. Online ahead of print.ABSTRACTPeople living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the development of these HIV-1-associated conditions. The HIV-1 matrix protein p17 (p17) is released and accumulates in different...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Alberto Zani Serena Messali Matteo Uggeri Carlo Bonfanti Arnaldo Caruso Francesca Caccuri Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Detection of HIV-1 matrix protein p17 in sera of viremic or aviremic patients
J Virol Methods. 2023 Nov 27:114858. doi: 10.1016/j.jviromet.2023.114858. Online ahead of print.ABSTRACTPeople living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the development of these HIV-1-associated conditions. The HIV-1 matrix protein p17 (p17) is released and accumulates in different...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Alberto Zani Serena Messali Matteo Uggeri Carlo Bonfanti Arnaldo Caruso Francesca Caccuri Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Detection of HIV-1 matrix protein p17 in sera of viremic or aviremic patients
J Virol Methods. 2023 Nov 27:114858. doi: 10.1016/j.jviromet.2023.114858. Online ahead of print.ABSTRACTPeople living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the development of these HIV-1-associated conditions. The HIV-1 matrix protein p17 (p17) is released and accumulates in different...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Alberto Zani Serena Messali Matteo Uggeri Carlo Bonfanti Arnaldo Caruso Francesca Caccuri Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Screening and identification of B cell epitope within the major capsid protein L1 of HPV 52, using monoclonal antibodies
In this study, HPV52 L1 protein was induced to be expressed. Monoclonal antibody (mAb) 6A7 against L1 protein were screened by cell fusion techniques. Western Blot and immunofluorescence assay (IFA) demonstrated the specificity of the mAb. The L1 protein was truncated for prokaryotic expression (N1~N7) and Dot-ELISA showed that 6A7 recognized N3 (aa 200~350). The immunodominant regions were truncated again for expression, with 6A7 recognizing N6 (aa 251~305). The N6 proteins were further truncated and then were constructed an four-segment eukaryotic expression vector. IFA showed that 6A7 could recognize amino acid 262~279....
Source: Journal of Virological Methods - November 28, 2023 Category: Virology Authors: Yumei Chen Shan Zhang Gaiping Zhang Jingming Zhou Hongliang Liu Chao Liang Enping Liu Xifang Zhu Aiping Wang Source Type: research
Screening and identification of B cell epitope within the major capsid protein L1 of HPV 52, using monoclonal antibodies
In this study, HPV52 L1 protein was induced to be expressed. Monoclonal antibody (mAb) 6A7 against L1 protein were screened by cell fusion techniques. Western Blot and immunofluorescence assay (IFA) demonstrated the specificity of the mAb. The L1 protein was truncated for prokaryotic expression (N1~N7) and Dot-ELISA showed that 6A7 recognized N3 (aa 200~350). The immunodominant regions were truncated again for expression, with 6A7 recognizing N6 (aa 251~305). The N6 proteins were further truncated and then were constructed an four-segment eukaryotic expression vector. IFA showed that 6A7 could recognize amino acid 262~279....
Source: Journal of Virological Methods - November 28, 2023 Category: Virology Authors: Yumei Chen Shan Zhang Gaiping Zhang Jingming Zhou Hongliang Liu Chao Liang Enping Liu Xifang Zhu Aiping Wang Source Type: research
Screening and identification of B cell epitope within the major capsid protein L1 of HPV 52, using monoclonal antibodies
In this study, HPV52 L1 protein was induced to be expressed. Monoclonal antibody (mAb) 6A7 against L1 protein were screened by cell fusion techniques. Western Blot and immunofluorescence assay (IFA) demonstrated the specificity of the mAb. The L1 protein was truncated for prokaryotic expression (N1~N7) and Dot-ELISA showed that 6A7 recognized N3 (aa 200~350). The immunodominant regions were truncated again for expression, with 6A7 recognizing N6 (aa 251~305). The N6 proteins were further truncated and then were constructed an four-segment eukaryotic expression vector. IFA showed that 6A7 could recognize amino acid 262~279....
Source: Journal of Virological Methods - November 28, 2023 Category: Virology Authors: Yumei Chen Shan Zhang Gaiping Zhang Jingming Zhou Hongliang Liu Chao Liang Enping Liu Xifang Zhu Aiping Wang Source Type: research
