Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18;322:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and effic...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18;322:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and effic...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and efficient...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and efficient...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1)
J Virol Methods. 2023 Sep 16:114813. doi: 10.1016/j.jviromet.2023.114813. Online ahead of print.ABSTRACTNewcastle disease (ND) caused by virulent avian paramyxovirus type I (APMV-1) is a WOAH and EU listed disease affecting poultry worldwide. ND exhibits different clinical manifestations that may either be neurological, respiratory and/or gastrointestinal, accompanied by high mortality. In contrast, mild or subclinical forms are generally caused by lentogenic APMV-1 and are not subject to notification. The rapid discrimination of virulent and avirulent viruses is paramount to limit the spread of virulent APMV-1. The approp...
Source: Journal of Virological Methods - September 18, 2023 Category: Virology Authors: Andrea Fortin Andrea Laconi Isabella Monne Siamak Zohari Kristofer Andersson Christian Grund Mattia Cecchinato Marika Crimaudo Viviana Valastro Valeria D'Amico Alessio Bortolami Michele Gastaldelli Maria Varotto Newcastle Disease Collaborating Diagnostic Source Type: research
A novel array of real-time RT-PCR assays for the rapid pathotyping of type I avian paramyxovirus (APMV-1)
J Virol Methods. 2023 Sep 16:114813. doi: 10.1016/j.jviromet.2023.114813. Online ahead of print.ABSTRACTNewcastle disease (ND) caused by virulent avian paramyxovirus type I (APMV-1) is a WOAH and EU listed disease affecting poultry worldwide. ND exhibits different clinical manifestations that may either be neurological, respiratory and/or gastrointestinal, accompanied by high mortality. In contrast, mild or subclinical forms are generally caused by lentogenic APMV-1 and are not subject to notification. The rapid discrimination of virulent and avirulent viruses is paramount to limit the spread of virulent APMV-1. The approp...
Source: Journal of Virological Methods - September 18, 2023 Category: Virology Authors: Andrea Fortin Andrea Laconi Isabella Monne Siamak Zohari Kristofer Andersson Christian Grund Mattia Cecchinato Marika Crimaudo Viviana Valastro Valeria D'Amico Alessio Bortolami Michele Gastaldelli Maria Varotto Newcastle Disease Collaborating Diagnostic Source Type: research
Diagnostic evaluation of Panbio ™ antigen rapid diagnostic test for SARS-CoV-2: A systematic review and meta-analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Diagnostic evaluation of Panbio ™ antigen rapid diagnostic test for SARS-CoV-2: A systematic review and meta-analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Diagnostic evaluation of Panbio ™ antigen rapid diagnostic test for SARS-CoV-2: A systematic review and meta-analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Diagnostic Evaluation of Panbio ™ Antigen Rapid Diagnostic Test for SARS-CoV-2: A Systematic Review and Meta-Analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Diagnostic Evaluation of Panbio ™ Antigen Rapid Diagnostic Test for SARS-CoV-2: A Systematic Review and Meta-Analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Diagnostic Evaluation of Panbio ™ Antigen Rapid Diagnostic Test for SARS-CoV-2: A Systematic Review and Meta-Analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Development and validation of a RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples
J Virol Methods. 2023 Sep 8:114808. doi: 10.1016/j.jviromet.2023.114808. Online ahead of print.ABSTRACTEpizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has become a concern in the European Union as a novel strain of the serotype 8 (EHDV-8) of clear Northern African origin, has been recently discovered in symptomatic cattle in Italy (islands of Sardinia and Sicily), Spain, and Portugal. Current molecular typing methods targeting the S2 nucleotide sequences -coding for the outermost protein of the virion-VP2- are not able to detect the novel emerging EHDV-8 st...
Source: Journal of Virological Methods - September 10, 2023 Category: Virology Authors: Ottavio Portanti Sarah Thabet Elena Abenza Eugenia Ciarrocchi Maura Pisciella Roberta Irelli Giovanni Savini Salah Hammami Simone Pulsoni Claudia Casaccia Lauren Coetzee Maurilia Marcacci Marco Di Domenico Alessio Lorusso Source Type: research
Development and validation of a RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples
J Virol Methods. 2023 Sep 8:114808. doi: 10.1016/j.jviromet.2023.114808. Online ahead of print.ABSTRACTEpizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has become a concern in the European Union as a novel strain of the serotype 8 (EHDV-8) of clear Northern African origin, has been recently discovered in symptomatic cattle in Italy (islands of Sardinia and Sicily), Spain, and Portugal. Current molecular typing methods targeting the S2 nucleotide sequences -coding for the outermost protein of the virion-VP2- are not able to detect the novel emerging EHDV-8 st...
Source: Journal of Virological Methods - September 10, 2023 Category: Virology Authors: Ottavio Portanti Sarah Thabet Elena Abenza Eugenia Ciarrocchi Maura Pisciella Roberta Irelli Giovanni Savini Salah Hammami Simone Pulsoni Claudia Casaccia Lauren Coetzee Maurilia Marcacci Marco Di Domenico Alessio Lorusso Source Type: research
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus
J Virol Methods. 2023 Sep 7:114810. doi: 10.1016/j.jviromet.2023.114810. Online ahead of print.ABSTRACTDuck Tembusu virus disease, caused by duck Tembusu virus (DTMUV), brings great harm to duck industry. Early diagnosis is of great significance for the prevention and control of this disease. In order to develop a specific and sensitive method for rapid diagnosis of DTMUV, reverse-transcriptase recombinase aided amplification combined with lateral flow dipstick (RT-RAA-LFD) method for detection of DTMUV was established. Firstly, downstream primer was labeled with biotin and probe was labeled with FAM, and primer concentrat...
Source: Journal of Virological Methods - September 9, 2023 Category: Virology Authors: Yixin Wang Xue Zhang Zhenyan Zhang Ruixue Xu Pingping Yang Peng Yuan Lianzhi Zhang Yanshun Cui Zhijing Xie Jianliang Li Source Type: research
