Corrigendum to "Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections" J. Virol. Methods 206 (2014) 38-41
J Virol Methods. 2023 Aug 25;321:114803. doi: 10.1016/j.jviromet.2023.114803. Online ahead of print.NO ABSTRACTPMID:37634273 | DOI:10.1016/j.jviromet.2023.114803 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - August 27, 2023 Category: Virology Authors: Alonzo Alfaro-N úñez M Thomas P Gilbert Source Type: research
Corrigendum to "Validation of a sensitive PCR assay for the detection of Chelonid fibropapilloma-associated herpesvirus in latent turtle infections" J. Virol. Methods 206 (2014) 38-41
J Virol Methods. 2023 Aug 25;321:114803. doi: 10.1016/j.jviromet.2023.114803. Online ahead of print.NO ABSTRACTPMID:37634273 | DOI:10.1016/j.jviromet.2023.114803 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - August 27, 2023 Category: Virology Authors: Alonzo Alfaro-N úñez M Thomas P Gilbert Source Type: research
Optimizing Virus Inactivation Methods for Molecular Detection Techniques: Implications for Viral Protein and RNA Measurements
J Virol Methods. 2023 Aug 23:114801. doi: 10.1016/j.jviromet.2023.114801. Online ahead of print.ABSTRACTTo facilitate the development of effective viral detection techniques, a positive control material is required for validating their quantitative performance. Inactivated viruses serve as viable control materials, as they can be handled without the constraints of biohazard safety facilities. However, inactivation alters the structure of viral component molecules, necessitating the selection of inactivation methods that have minimal effects on the target molecules relevant to molecular detection techniques. Only a limited ...
Source: Journal of Virological Methods - August 25, 2023 Category: Virology Authors: Takema Hasegawa Sachie Shibayama Yukiko Osumi Megumi Kato Source Type: research
Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification
J Virol Methods. 2023 Aug 23:114802. doi: 10.1016/j.jviromet.2023.114802. Online ahead of print.ABSTRACTQuantification of mpox virus (MPXV) across different human body anatomical sites can provide insights about the most likely transmission routes, so methods able to release absolute and exact quantitative values of MPXV DNA are crucial. Here, we optimized a new QIAcuity digital PCR (dPCR) protocol for the detection and quantification of MPXV DNA in clinical samples and assessed the performance of the assay by comparing the results obtained in 144 biological samples with those resulting from the use of an in-house real-tim...
Source: Journal of Virological Methods - August 25, 2023 Category: Virology Authors: Eliana Specchiarello Fabrizio Carletti Giulia Matusali Isabella Abbate Gabriella Rozera Claudia Minosse Elisabetta Petrivelli Valeria Ferraioli Roberta Sciamanna Fabrizio Maggi Source Type: research
Optimizing Virus Inactivation Methods for Molecular Detection Techniques: Implications for Viral Protein and RNA Measurements
J Virol Methods. 2023 Aug 23:114801. doi: 10.1016/j.jviromet.2023.114801. Online ahead of print.ABSTRACTTo facilitate the development of effective viral detection techniques, a positive control material is required for validating their quantitative performance. Inactivated viruses serve as viable control materials, as they can be handled without the constraints of biohazard safety facilities. However, inactivation alters the structure of viral component molecules, necessitating the selection of inactivation methods that have minimal effects on the target molecules relevant to molecular detection techniques. Only a limited ...
Source: Journal of Virological Methods - August 25, 2023 Category: Virology Authors: Takema Hasegawa Sachie Shibayama Yukiko Osumi Megumi Kato Source Type: research
Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification
J Virol Methods. 2023 Aug 23:114802. doi: 10.1016/j.jviromet.2023.114802. Online ahead of print.ABSTRACTQuantification of mpox virus (MPXV) across different human body anatomical sites can provide insights about the most likely transmission routes, so methods able to release absolute and exact quantitative values of MPXV DNA are crucial. Here, we optimized a new QIAcuity digital PCR (dPCR) protocol for the detection and quantification of MPXV DNA in clinical samples and assessed the performance of the assay by comparing the results obtained in 144 biological samples with those resulting from the use of an in-house real-tim...
Source: Journal of Virological Methods - August 25, 2023 Category: Virology Authors: Eliana Specchiarello Fabrizio Carletti Giulia Matusali Isabella Abbate Gabriella Rozera Claudia Minosse Elisabetta Petrivelli Valeria Ferraioli Roberta Sciamanna Fabrizio Maggi Source Type: research
Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples
We presently deplete chicken- and selected bacterial-specific rRNAs in poultry field RNA samples with complementary DNA probes in a commercially available probe hybridization buffer followed by digestion of the RNA:DNA hybrids with RNase H. Because the current buffer is an expensive special order reagent of proprietary composition, we tested in-house and other commercially available buffers and identified a viable alternative that yields equivalent host rRNA depletion and viral-specific reads in poultry samples as the current special order reagent but at a reduced cost.PMID:37604238 | DOI:10.1016/j.jviromet.2023.114793 (So...
Source: Journal of Virological Methods - August 21, 2023 Category: Virology Authors: Abhijeet Bakre Henry M Karithii David L Suarez Source Type: research
Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples
We presently deplete chicken- and selected bacterial-specific rRNAs in poultry field RNA samples with complementary DNA probes in a commercially available probe hybridization buffer followed by digestion of the RNA:DNA hybrids with RNase H. Because the current buffer is an expensive special order reagent of proprietary composition, we tested in-house and other commercially available buffers and identified a viable alternative that yields equivalent host rRNA depletion and viral-specific reads in poultry samples as the current special order reagent but at a reduced cost.PMID:37604238 | DOI:10.1016/j.jviromet.2023.114793 (So...
Source: Journal of Virological Methods - August 21, 2023 Category: Virology Authors: Abhijeet Bakre Henry M Karithii David L Suarez Source Type: research
Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples
We presently deplete chicken- and selected bacterial-specific rRNAs in poultry field RNA samples with complementary DNA probes in a commercially available probe hybridization buffer followed by digestion of the RNA:DNA hybrids with RNase H. Because the current buffer is an expensive special order reagent of proprietary composition, we tested in-house and other commercially available buffers and identified a viable alternative that yields equivalent host rRNA depletion and viral-specific reads in poultry samples as the current special order reagent but at a reduced cost.PMID:37604238 | DOI:10.1016/j.jviromet.2023.114793 (So...
Source: Journal of Virological Methods - August 21, 2023 Category: Virology Authors: Abhijeet Bakre Henry M Karithii David L Suarez Source Type: research
Alternative probe hybridization buffers for target RNA depletion and viral sequence recovery in NGS for poultry samples
We presently deplete chicken- and selected bacterial-specific rRNAs in poultry field RNA samples with complementary DNA probes in a commercially available probe hybridization buffer followed by digestion of the RNA:DNA hybrids with RNase H. Because the current buffer is an expensive special order reagent of proprietary composition, we tested in-house and other commercially available buffers and identified a viable alternative that yields equivalent host rRNA depletion and viral-specific reads in poultry samples as the current special order reagent but at a reduced cost.PMID:37604238 | DOI:10.1016/j.jviromet.2023.114793 (So...
Source: Journal of Virological Methods - August 21, 2023 Category: Virology Authors: Abhijeet Bakre Henry M Karithii David L Suarez Source Type: research
Successful Transduction of Target Gene Mediated by Adeno-Associated Virus 2 into Lens Epithelial Cells in Rats
In this study, we compared the efficiency of intravitreal injection of six AAV serotypes (AAV2, AAV5, AAV6, AAV8, AAV9, and AAVDJ) to transduce lens and retina in rats, The expression and localization of the reporter gene ZsGreen in the lens and retina were examined using immunofluorescence staining, and the relative expression of ZsGreen mRNA was detected using RT-qPCR. Our results demonstrated that AAV2 had the highest efficiency in transducing LECs. All six AAV serotypes could transduce the retina. To validate this observation, we further constructed an AAV2 vector with exogenous gene senescence marker protein 30 (SMP30...
Source: Journal of Virological Methods - August 17, 2023 Category: Virology Authors: Yongshun Liang Tian Lan Qingqiao Gan Hao Liang Source Type: research
Successful Transduction of Target Gene Mediated by Adeno-Associated Virus 2 into Lens Epithelial Cells in Rats
In this study, we compared the efficiency of intravitreal injection of six AAV serotypes (AAV2, AAV5, AAV6, AAV8, AAV9, and AAVDJ) to transduce lens and retina in rats, The expression and localization of the reporter gene ZsGreen in the lens and retina were examined using immunofluorescence staining, and the relative expression of ZsGreen mRNA was detected using RT-qPCR. Our results demonstrated that AAV2 had the highest efficiency in transducing LECs. All six AAV serotypes could transduce the retina. To validate this observation, we further constructed an AAV2 vector with exogenous gene senescence marker protein 30 (SMP30...
Source: Journal of Virological Methods - August 17, 2023 Category: Virology Authors: Yongshun Liang Tian Lan Qingqiao Gan Hao Liang Source Type: research
Successful Transduction of Target Gene Mediated by Adeno-Associated Virus 2 into Lens Epithelial Cells in Rats
In this study, we compared the efficiency of intravitreal injection of six AAV serotypes (AAV2, AAV5, AAV6, AAV8, AAV9, and AAVDJ) to transduce lens and retina in rats, The expression and localization of the reporter gene ZsGreen in the lens and retina were examined using immunofluorescence staining, and the relative expression of ZsGreen mRNA was detected using RT-qPCR. Our results demonstrated that AAV2 had the highest efficiency in transducing LECs. All six AAV serotypes could transduce the retina. To validate this observation, we further constructed an AAV2 vector with exogenous gene senescence marker protein 30 (SMP30...
Source: Journal of Virological Methods - August 17, 2023 Category: Virology Authors: Yongshun Liang Tian Lan Qingqiao Gan Hao Liang Source Type: research
Validation of a TaqMan one-step real-time RT-PCR assay targeting ISAV segment 7 spliced mRNA
J Virol Methods. 2023 Aug 8:114791. doi: 10.1016/j.jviromet.2023.114791. Online ahead of print.ABSTRACTInfectious salmon anaemia virus (ISAV) can cause severe systemic infection in Atlantic salmon (Salmo salar L.), and a timely diagnosis is critical. Conventional real-time reverse transcription PCR (RT-qPCR) assays target unspliced RNA from either ISAV segment 7 or 8 and provide data on viral load. Here, we evaluate a TaqMan one-step RT-qPCR assay that detects explicitly a spliced messenger RNA (mRNA) of ISAV segment 7, thus providing evidence of active viral transcription. Assay performance was comparable with existing un...
Source: Journal of Virological Methods - August 10, 2023 Category: Virology Authors: Petra Elisabeth Petersen Maria Marjunard óttir Dahl Nicolina Maria Ovad óttir Vest Mona Dverdal Jansen Johanna Hol Fosse Knut Falk Debes Hammershaimb Christiansen Source Type: research
Validation of a TaqMan one-step real-time RT-PCR assay targeting ISAV segment 7 spliced mRNA
J Virol Methods. 2023 Aug 8:114791. doi: 10.1016/j.jviromet.2023.114791. Online ahead of print.ABSTRACTInfectious salmon anaemia virus (ISAV) can cause severe systemic infection in Atlantic salmon (Salmo salar L.), and a timely diagnosis is critical. Conventional real-time reverse transcription PCR (RT-qPCR) assays target unspliced RNA from either ISAV segment 7 or 8 and provide data on viral load. Here, we evaluate a TaqMan one-step RT-qPCR assay that detects explicitly a spliced messenger RNA (mRNA) of ISAV segment 7, thus providing evidence of active viral transcription. Assay performance was comparable with existing un...
Source: Journal of Virological Methods - August 10, 2023 Category: Virology Authors: Petra Elisabeth Petersen Maria Marjunard óttir Dahl Nicolina Maria Ovad óttir Vest Mona Dverdal Jansen Johanna Hol Fosse Knut Falk Debes Hammershaimb Christiansen Source Type: research
