Diagnostic Evaluation of Panbio ™ Antigen Rapid Diagnostic Test for SARS-CoV-2: A Systematic Review and Meta-Analysis
CONCLUSION: Panbio™ Ag-RDT presented an outstanding diagnostic accuracy in the detection of the SARS-CoV-2 virus in both adults and children with or without symptoms.PMID:37696303 | DOI:10.1016/j.jviromet.2023.114811 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - September 11, 2023 Category: Virology Authors: Safa Boujemaa Gurparsad Singh Suri Gurleen Kaur Source Type: research
Development and validation of a RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples
J Virol Methods. 2023 Sep 8:114808. doi: 10.1016/j.jviromet.2023.114808. Online ahead of print.ABSTRACTEpizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has become a concern in the European Union as a novel strain of the serotype 8 (EHDV-8) of clear Northern African origin, has been recently discovered in symptomatic cattle in Italy (islands of Sardinia and Sicily), Spain, and Portugal. Current molecular typing methods targeting the S2 nucleotide sequences -coding for the outermost protein of the virion-VP2- are not able to detect the novel emerging EHDV-8 st...
Source: Journal of Virological Methods - September 10, 2023 Category: Virology Authors: Ottavio Portanti Sarah Thabet Elena Abenza Eugenia Ciarrocchi Maura Pisciella Roberta Irelli Giovanni Savini Salah Hammami Simone Pulsoni Claudia Casaccia Lauren Coetzee Maurilia Marcacci Marco Di Domenico Alessio Lorusso Source Type: research
Development and validation of a RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples
J Virol Methods. 2023 Sep 8:114808. doi: 10.1016/j.jviromet.2023.114808. Online ahead of print.ABSTRACTEpizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has become a concern in the European Union as a novel strain of the serotype 8 (EHDV-8) of clear Northern African origin, has been recently discovered in symptomatic cattle in Italy (islands of Sardinia and Sicily), Spain, and Portugal. Current molecular typing methods targeting the S2 nucleotide sequences -coding for the outermost protein of the virion-VP2- are not able to detect the novel emerging EHDV-8 st...
Source: Journal of Virological Methods - September 10, 2023 Category: Virology Authors: Ottavio Portanti Sarah Thabet Elena Abenza Eugenia Ciarrocchi Maura Pisciella Roberta Irelli Giovanni Savini Salah Hammami Simone Pulsoni Claudia Casaccia Lauren Coetzee Maurilia Marcacci Marco Di Domenico Alessio Lorusso Source Type: research
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus
J Virol Methods. 2023 Sep 7:114810. doi: 10.1016/j.jviromet.2023.114810. Online ahead of print.ABSTRACTDuck Tembusu virus disease, caused by duck Tembusu virus (DTMUV), brings great harm to duck industry. Early diagnosis is of great significance for the prevention and control of this disease. In order to develop a specific and sensitive method for rapid diagnosis of DTMUV, reverse-transcriptase recombinase aided amplification combined with lateral flow dipstick (RT-RAA-LFD) method for detection of DTMUV was established. Firstly, downstream primer was labeled with biotin and probe was labeled with FAM, and primer concentrat...
Source: Journal of Virological Methods - September 9, 2023 Category: Virology Authors: Yixin Wang Xue Zhang Zhenyan Zhang Ruixue Xu Pingping Yang Peng Yuan Lianzhi Zhang Yanshun Cui Zhijing Xie Jianliang Li Source Type: research
Establishment of two visual interpretation methods of DIV1 LAMP amplification products
This study established loop-mediated isothermal amplification (LAMP) along with two visual interpretation methods, LAMP-dye and LAMP-LFD, to detect DIV1. The newly developed method would not cause cross-reactions with other shrimp pathogens such as white spot syndrome virus (WSSV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), Enterocytozoon hepatopenaei (EHP), and Vibrio parahaemolyticus acute hepatopancreatic necrosis disease (VpAHPND). The detection limit of DIV1 LAMP was as low as 103 copies of DIV1 per reaction, with a reaction time of less than 40minutes. The diagnostic sensitivity and diagnostic sp...
Source: Journal of Virological Methods - September 9, 2023 Category: Virology Authors: Chen Li Kaiting Jiang Liang Qiu Qingli Zhang Bing Yang Source Type: research
Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Test for PPRV Antibody
J Virol Methods. 2023 Sep 6:114809. doi: 10.1016/j.jviromet.2023.114809. Online ahead of print.ABSTRACTPeste des petits ruminants virus (PPRV) causes a very devastating disease in sheep and goats. Rapid diagnosis and immunisation have been identified as key strategies for successful prevention of the disease. Therefore, a sensitive fluorescent microsphere immunochromatography test strips (FM-ICTS) was developed for rapid detection of special antibodies of PPRV in goats and sheep serum. The FM-ICTS were successfully prepared by fluorescent microspheres (FM) as tracer, which were covalently coupled to PPRV nucleocapsid prote...
Source: Journal of Virological Methods - September 8, 2023 Category: Virology Authors: Shuai Dong Weiqin Meng Zhe Yang Jinlong Chen Jianchai Liu Zhiqiang Shen Jinliang Wang Source Type: research
A rapid and affordable amplicon-based method for next-generation genome sequencing of the infectious bursal disease virus
J Virol Methods. 2023 Sep 6:114807. doi: 10.1016/j.jviromet.2023.114807. Online ahead of print.ABSTRACTThe infectious bursal disease virus (IBDV) causes a severe immunosuppressive disorder in young chickens. IBDV evolution resulted in the emergence of strains with divergent genetic, antigenic, and pathogenic characteristics. Genetic classification is typically performed by sequencing the coding region of the most immunogenic region of the viral protein 2 (VP2). Sequencing both double-stranded RNA genome segments is essential to achieve a more comprehensive IBDV classification that can detect recombinants and reassortments....
Source: Journal of Virological Methods - September 8, 2023 Category: Virology Authors: Claudia Techera Gonzalo Tom ás Sof ía Grecco Joaqu ín Williman Mart ín Hernández Valeria Olivera Alejandro Banda Ariel Vagnozzi Yanina Panzera Ana Marandino Ruben P érez Source Type: research
Development of a Sensitive Immunochromatographic Method Using Lanthanide Fluorescent Microsphere for Rapid Test for PPRV Antibody
J Virol Methods. 2023 Sep 6:114809. doi: 10.1016/j.jviromet.2023.114809. Online ahead of print.ABSTRACTPeste des petits ruminants virus (PPRV) causes a very devastating disease in sheep and goats. Rapid diagnosis and immunisation have been identified as key strategies for successful prevention of the disease. Therefore, a sensitive fluorescent microsphere immunochromatography test strips (FM-ICTS) was developed for rapid detection of special antibodies of PPRV in goats and sheep serum. The FM-ICTS were successfully prepared by fluorescent microspheres (FM) as tracer, which were covalently coupled to PPRV nucleocapsid prote...
Source: Journal of Virological Methods - September 8, 2023 Category: Virology Authors: Shuai Dong Weiqin Meng Zhe Yang Jinlong Chen Jianchai Liu Zhiqiang Shen Jinliang Wang Source Type: research
A rapid and affordable amplicon-based method for next-generation genome sequencing of the infectious bursal disease virus
J Virol Methods. 2023 Sep 6:114807. doi: 10.1016/j.jviromet.2023.114807. Online ahead of print.ABSTRACTThe infectious bursal disease virus (IBDV) causes a severe immunosuppressive disorder in young chickens. IBDV evolution resulted in the emergence of strains with divergent genetic, antigenic, and pathogenic characteristics. Genetic classification is typically performed by sequencing the coding region of the most immunogenic region of the viral protein 2 (VP2). Sequencing both double-stranded RNA genome segments is essential to achieve a more comprehensive IBDV classification that can detect recombinants and reassortments....
Source: Journal of Virological Methods - September 8, 2023 Category: Virology Authors: Claudia Techera Gonzalo Tom ás Sof ía Grecco Joaqu ín Williman Mart ín Hernández Valeria Olivera Alejandro Banda Ariel Vagnozzi Yanina Panzera Ana Marandino Ruben P érez Source Type: research
Efficient artificial microRNA-mediated resistance against zucchini yellow mosaic virus in zucchini via agroinfiltration
J Virol Methods. 2023 Sep 4:114805. doi: 10.1016/j.jviromet.2023.114805. Online ahead of print.ABSTRACTZucchini yellow mosaic virus (ZYMV) infects cucurbits causing yellow mosaic in leaves, malformations in fruits, and degradation of the product quality. RNA interference (RNAi) is a cellular mechanism in eukaryotes and it is exploited to protect them against viruses. The artificial micro RNA (amiRNA) mediated approach was employed to develop resistance against ZYMV. Four amiRNAs, amiZYMV_HC-115s and amiZYMV_HC-1162s (sense), amiZYMV_HC-182as and amiZYMV_HC-196as (antisense), were computationally designed and introduced int...
Source: Journal of Virological Methods - September 6, 2023 Category: Virology Authors: Margarita Berbati Athanasios Kaldis Andreas Voloudakis Source Type: research
Efficient artificial microRNA-mediated resistance against zucchini yellow mosaic virus in zucchini via agroinfiltration
J Virol Methods. 2023 Sep 4:114805. doi: 10.1016/j.jviromet.2023.114805. Online ahead of print.ABSTRACTZucchini yellow mosaic virus (ZYMV) infects cucurbits causing yellow mosaic in leaves, malformations in fruits, and degradation of the product quality. RNA interference (RNAi) is a cellular mechanism in eukaryotes and it is exploited to protect them against viruses. The artificial micro RNA (amiRNA) mediated approach was employed to develop resistance against ZYMV. Four amiRNAs, amiZYMV_HC-115s and amiZYMV_HC-1162s (sense), amiZYMV_HC-182as and amiZYMV_HC-196as (antisense), were computationally designed and introduced int...
Source: Journal of Virological Methods - September 6, 2023 Category: Virology Authors: Margarita Berbati Athanasios Kaldis Andreas Voloudakis Source Type: research
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples
In this study, we developed a one-step duplex RT-qPCR assay to simultaneously test the two main human pathogenic NoV genogroups, GI and GII, in wastewater samples. The assay had low limits of detection (LOD), namely 0.52 genome copies (gc)/µl for NoVGI and 1.37 gc/µl for NoVGII. No significant concentration-dependent interactions were noted for both NoVGI and for NoVGII when the two targets were mixed at different concentrations in the samples. When tested on wastewater-derived RNA eluents, no significant difference between duplex and singleplex concentrations were found for either target. Low levels of inhibition (up to...
Source: Journal of Virological Methods - August 29, 2023 Category: Virology Authors: Natasha Alex-Sanders Nick Woodhall Kata Farkas George Scott Davey L Jones David I Walker Source Type: research
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples
In this study, we developed a one-step duplex RT-qPCR assay to simultaneously test the two main human pathogenic NoV genogroups, GI and GII, in wastewater samples. The assay had low limits of detection (LOD), namely 0.52 genome copies (gc)/µl for NoVGI and 1.37 gc/µl for NoVGII. No significant concentration-dependent interactions were noted for both NoVGI and for NoVGII when the two targets were mixed at different concentrations in the samples. When tested on wastewater-derived RNA eluents, no significant difference between duplex and singleplex concentrations were found for either target. Low levels of inhibition (up to...
Source: Journal of Virological Methods - August 29, 2023 Category: Virology Authors: Natasha Alex-Sanders Nick Woodhall Kata Farkas George Scott Davey L Jones David I Walker Source Type: research
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples
In this study, we developed a one-step duplex RT-qPCR assay to simultaneously test the two main human pathogenic NoV genogroups, GI and GII, in wastewater samples. The assay had low limits of detection (LOD), namely 0.52 genome copies (gc)/µl for NoVGI and 1.37 gc/µl for NoVGII. No significant concentration-dependent interactions were noted for both NoVGI and for NoVGII when the two targets were mixed at different concentrations in the samples. When tested on wastewater-derived RNA eluents, no significant difference between duplex and singleplex concentrations were found for either target. Low levels of inhibition (up to...
Source: Journal of Virological Methods - August 29, 2023 Category: Virology Authors: Natasha Alex-Sanders Nick Woodhall Kata Farkas George Scott Davey L Jones David I Walker Source Type: research
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples
In this study, we developed a one-step duplex RT-qPCR assay to simultaneously test the two main human pathogenic NoV genogroups, GI and GII, in wastewater samples. The assay had low limits of detection (LOD), namely 0.52 genome copies (gc)/µl for NoVGI and 1.37 gc/µl for NoVGII. No significant concentration-dependent interactions were noted for both NoVGI and for NoVGII when the two targets were mixed at different concentrations in the samples. When tested on wastewater-derived RNA eluents, no significant difference between duplex and singleplex concentrations were found for either target. Low levels of inhibition (up to...
Source: Journal of Virological Methods - August 29, 2023 Category: Virology Authors: Natasha Alex-Sanders Nick Woodhall Kata Farkas George Scott Davey L Jones David I Walker Source Type: research
