Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)
J Virol Methods. 2024 Mar 29:114922. doi: 10.1016/j.jviromet.2024.114922. Online ahead of print.ABSTRACTA 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell layers. Upon challenge with NNV in a setup containing both autoclaved salt water and L15 media in the apical compartment, viral replication akin to that anticipated based on previous studies was observed. Consequently, we advocat...
Source: Journal of Virological Methods - March 31, 2024 Category: Virology Authors: Rahul Rajendran Rahul Krishnan Myung-Joo Oh Source Type: research
Improved SARS-CoV-2 RNA Recovery in Wastewater Matrices using a CTAB-based Extraction Method
J Virol Methods. 2024 Mar 29:114918. doi: 10.1016/j.jviromet.2024.114918. Online ahead of print.ABSTRACTWastewater-based epidemiology has allowed tracking the magnitude and distribution of SARS-CoV-2 in communities, allowing public health officials to prepare for impending outbreaks. While many factors influence recovery of SARS-CoV-2 from wastewater, proper extraction, concentration, and purification of RNA are key steps to ensure accurate detection of viral particles. The aim of this study was to compare the efficiency of four commonly used RNA extraction methods for detection of the SARS-CoV-2 RNA genome in sewage sampl...
Source: Journal of Virological Methods - March 31, 2024 Category: Virology Authors: Mar ía Julia Ousset Luis Alfredo Pianciola Melina Mazzeo Juan Mart ín Oteiza Mar ía Soledad Jaureguiberry Andr és Venturino Patricia Ang élica Barril Source Type: research
Optimal Conditions for Adenoviral Transduction of Immature Dendritic Cells Without Affecting the Tolerogenic Activity of DC-Based Immunotherapy
J Virol Methods. 2024 Mar 27:114921. doi: 10.1016/j.jviromet.2024.114921. Online ahead of print.ABSTRACTDendritic cells (DCs) play a pivotal role in maintaining immune tolerance. Using recombinant adenovirus (rAd) to deliver vectors to immature dendritic cells (imDCs) is an important method for studying the tolerogenic function of DCs. We found that using RPMI medium and a higher MOI during transduction increased the expression of CD80, CD86, and MHC-II on the surface of imDCs. Our data reveal a significant increase in the secretion of the pro-inflammatory cytokine IL-6 in the group showing the most pronounced phenotypic c...
Source: Journal of Virological Methods - March 29, 2024 Category: Virology Authors: Qian Jian Zongli Fu Hanyu Wang Hanyuan Zhang Yi Ma Source Type: research
Optimal Conditions for Adenoviral Transduction of Immature Dendritic Cells Without Affecting the Tolerogenic Activity of DC-Based Immunotherapy
J Virol Methods. 2024 Mar 27:114921. doi: 10.1016/j.jviromet.2024.114921. Online ahead of print.ABSTRACTDendritic cells (DCs) play a pivotal role in maintaining immune tolerance. Using recombinant adenovirus (rAd) to deliver vectors to immature dendritic cells (imDCs) is an important method for studying the tolerogenic function of DCs. We found that using RPMI medium and a higher MOI during transduction increased the expression of CD80, CD86, and MHC-II on the surface of imDCs. Our data reveal a significant increase in the secretion of the pro-inflammatory cytokine IL-6 in the group showing the most pronounced phenotypic c...
Source: Journal of Virological Methods - March 29, 2024 Category: Virology Authors: Qian Jian Zongli Fu Hanyu Wang Hanyuan Zhang Yi Ma Source Type: research
The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
The Effect of Enzymatic and Viability Dye Treatment in Combination with Long-Range PCR on Assessing Tulane Virus Infectivity
J Virol Methods. 2024 Mar 24:114919. doi: 10.1016/j.jviromet.2024.114919. Online ahead of print.ABSTRACTHuman norovirus (HuNoV) is regularly involved in food-borne infections. To detect infectious HuNoV in food, RT-qPCR remains state of the art but also amplifies non-infectious virus. The present study combines pre-treatments, RNase and propidium monoazide, with three molecular analyses, including long-range PCR, to predominantly detect infectious Tulane virus (TuV), a culturable HuNoV surrogate. TuV was exposed to inactivating conditions to assess which molecular method most closely approximates the reduction in infectiou...
Source: Journal of Virological Methods - March 26, 2024 Category: Virology Authors: Sarah M Stoppel Bj ørn Tore Lunestad Mette Myrmel Source Type: research
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
J Virol Methods. 2024 Mar 17:114917. doi: 10.1016/j.jviromet.2024.114917. Online ahead of print.ABSTRACTBagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and the Middle East. The virus has been associated with neurological disease and fatalities in various wild bird species. Association with human disease is not confirmed although limited serological evidence has suggested human infection. Surveillance programs for screening mosquitoes for evidence of arbovirus infection play an important role in providing information regarding the circulation and spread of ...
Source: Journal of Virological Methods - March 19, 2024 Category: Virology Authors: T R Sekee R Bubuluma D van Jaarsveldt P A Bester F J Burt Source Type: research
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
J Virol Methods. 2024 Mar 17:114917. doi: 10.1016/j.jviromet.2024.114917. Online ahead of print.ABSTRACTBagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and the Middle East. The virus has been associated with neurological disease and fatalities in various wild bird species. Association with human disease is not confirmed although limited serological evidence has suggested human infection. Surveillance programs for screening mosquitoes for evidence of arbovirus infection play an important role in providing information regarding the circulation and spread of ...
Source: Journal of Virological Methods - March 19, 2024 Category: Virology Authors: T R Sekee R Bubuluma D van Jaarsveldt P A Bester F J Burt Source Type: research
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
J Virol Methods. 2024 Mar 17:114917. doi: 10.1016/j.jviromet.2024.114917. Online ahead of print.ABSTRACTBagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and the Middle East. The virus has been associated with neurological disease and fatalities in various wild bird species. Association with human disease is not confirmed although limited serological evidence has suggested human infection. Surveillance programs for screening mosquitoes for evidence of arbovirus infection play an important role in providing information regarding the circulation and spread of ...
Source: Journal of Virological Methods - March 19, 2024 Category: Virology Authors: T R Sekee R Bubuluma D van Jaarsveldt P A Bester F J Burt Source Type: research
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
J Virol Methods. 2024 Mar 17:114917. doi: 10.1016/j.jviromet.2024.114917. Online ahead of print.ABSTRACTBagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and the Middle East. The virus has been associated with neurological disease and fatalities in various wild bird species. Association with human disease is not confirmed although limited serological evidence has suggested human infection. Surveillance programs for screening mosquitoes for evidence of arbovirus infection play an important role in providing information regarding the circulation and spread of ...
Source: Journal of Virological Methods - March 19, 2024 Category: Virology Authors: T R Sekee R Bubuluma D van Jaarsveldt P A Bester F J Burt Source Type: research
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
J Virol Methods. 2024 Mar 17:114917. doi: 10.1016/j.jviromet.2024.114917. Online ahead of print.ABSTRACTBagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and the Middle East. The virus has been associated with neurological disease and fatalities in various wild bird species. Association with human disease is not confirmed although limited serological evidence has suggested human infection. Surveillance programs for screening mosquitoes for evidence of arbovirus infection play an important role in providing information regarding the circulation and spread of ...
Source: Journal of Virological Methods - March 19, 2024 Category: Virology Authors: T R Sekee R Bubuluma D van Jaarsveldt P A Bester F J Burt Source Type: research
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa
J Virol Methods. 2024 Mar 17:114917. doi: 10.1016/j.jviromet.2024.114917. Online ahead of print.ABSTRACTBagaza virus (BAGV) is a mosquito-borne orthoflavivirus known to occur in regions of southern Europe, Africa, India and the Middle East. The virus has been associated with neurological disease and fatalities in various wild bird species. Association with human disease is not confirmed although limited serological evidence has suggested human infection. Surveillance programs for screening mosquitoes for evidence of arbovirus infection play an important role in providing information regarding the circulation and spread of ...
Source: Journal of Virological Methods - March 19, 2024 Category: Virology Authors: T R Sekee R Bubuluma D van Jaarsveldt P A Bester F J Burt Source Type: research
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.PMID:38479084 | DOI:10.1016/j.jviromet.2024.114906 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - March 13, 2024 Category: Virology Authors: Uzma Jabeen Kailash Singh Bisht Huildore Bommanna Ranjitha Madhusudan Hosamani Beeragere Parameshwaraiah Sreenivasa Pratik M Kulkarni Dombesara Chandrashekar Nidhi Rajegowdanadoddi Lakshmana Amulya Veerakyathappa Bhanuprakash Hosur Joyappa Dechamma Aniket Source Type: research
