Journal of Industrial Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Computer-Aided, Resistance Gene-Guided Genome Mining for Proteasome and HMG-CoA Reductase Inhibitors
J Ind Microbiol Biotechnol. 2023 Dec 7:kuad045. doi: 10.1093/jimb/kuad045. Online ahead of print.ABSTRACTSecondary metabolites (SMs) are biologically active small molecules, many of which are medically valuable. Fungal genomes contain vast numbers of SM biosynthetic gene clusters (BGCs) with unknown products, suggesting that huge numbers of valuable SMs remain to be discovered. It is challenging, however, to identify SM BGCs, among the millions present in fungi, that produce useful compounds. One solution is resistance gene-guided genome mining, which takes advantage of the fact that some BGCs contain a gene encoding a res...
Source: Journal of Industrial Microbiology and Biotechnology - December 7, 2023 Category: Microbiology Authors: Cory B Jenkinson Adam R Podgorny Cuncong Zhong Berl R Oakley Source Type: research

Lessons from assembling a microbial natural product and pre-fractionated extract library in an academic laboratory
J Ind Microbiol Biotechnol. 2023 Dec 5:kuad042. doi: 10.1093/jimb/kuad042. Online ahead of print.ABSTRACTMicrobial natural products are specialized metabolites that are sources of many bioactive compounds including antibiotics, antifungals, antiparasitics, anticancer agents, and probes of biology. The assembly of libraries of producers of natural products has traditionally been the province of the pharmaceutical industry. This sector has gathered significant historical collections of bacteria and fungi to identify new drug leads with outstanding outcomes-upwards of 60% of drug scaffolds originate from such libraries. Despi...
Source: Journal of Industrial Microbiology and Biotechnology - December 5, 2023 Category: Microbiology Authors: Michael A Cook Daniel Pallant Linda Ejim Arlene D Sutherland Xiaodong Wang Jarrod W Johnson Susan McCusker Xuefei Chen Maya George Sommer Chou Kalinka Koteva Wenliang Wang Christian Hobson Dirk Hackenberger Nicholas Waglechner Obie Ejim Tracey Campbell Ri Source Type: research

Bacterial natural product discovery by heterologous expression
J Ind Microbiol Biotechnol. 2023 Dec 5:kuad044. doi: 10.1093/jimb/kuad044. Online ahead of print.ABSTRACTNatural products have found important applications in the pharmaceutical and agricultural sectors. In bacteria, the genes that encode the biosynthesis of natural products are often colocalized in the genome, forming biosynthetic gene clusters. It has been predicted that only 3% of natural products encoded in bacterial genomes have been discovered thus far, in part because gene clusters may be poorly expressed under laboratory conditions. Heterologous expression can help convert bioinformatics predictions into products. ...
Source: Journal of Industrial Microbiology and Biotechnology - December 5, 2023 Category: Microbiology Authors: Adjo E Kadjo Alessandra S Eust áquio Source Type: research

Characterization and Catalytic Investigation of Fungal Single-Module Nonribosomal Peptide Synthetase in Terpene-Amino Acid Meroterpenoid Biosynthesis
In this study, we performed biochemical characterization of AneB by in vitro characterization, molecular docking and site-directed mutagenesis. The enzyme reaction analyses, performed with L-proline and daucane/nordaucane sesquiterpene substrates, revealed that AneB specifically esterifies the C10-OH of aculenes with L-proline. Notably, in contrast to ThmA in CJ-12662 biosynthesis, which exclusively recognizes oxygenated amorpha-4,11-diene sesquiterpenes for L-tryptophan transfer, AneB demonstrates broad substrate selectivity, including oxygenated amorpha-4,11-diene and 2-phenylethanol, resulting in the production of diver...
Source: Journal of Industrial Microbiology and Biotechnology - December 4, 2023 Category: Microbiology Authors: Cheng-Chung Tseng Li-Xun Chen Chi-Fang Lee Zhijay Tu Chun-Hung Lin Hsiao-Ching Lin Source Type: research

Determining mating type and ploidy in Rhodotorula toruloides and its effect on growth on sugars from lignocellulosic biomass
J Ind Microbiol Biotechnol. 2023 Feb 17;50(1):kuad040. doi: 10.1093/jimb/kuad040.ABSTRACTRhodotorula toruloides is being developed for the use in industrial biotechnology processes because of its favorable physiology. This includes its ability to produce and store large amounts of lipids in the form of intracellular lipid bodies. Nineteen strains were characterized for mating type, ploidy, robustness for growth, and accumulation of lipids on inhibitory switchgrass hydrolysate (SGH). Mating type was determined using a novel polymerase chain reaction (PCR)-based assay, which was validated using the classical microscopic test...
Source: Journal of Industrial Microbiology and Biotechnology - November 21, 2023 Category: Microbiology Authors: Daiane Dias Lopes Bruce S Dien Ronald E Hector Vijay Singh Stephanie R Thompson Patricia J Slininger Kyria Boundy-Mills Sujit S Jagtap Christopher V Rao Source Type: research