Journal of Industrial Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Perkinsus marinus in bioreactor: Growth and a cost-reduced growth medium
This study opens the door to furthering P. marinus as a valid heterologous expression system by showing the ability to grow in bioreactors.PMID:37669897 | DOI:10.1093/jimb/kuad023 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - September 5, 2023 Category: Microbiology Authors: Caitlin Murphy Jos é A Fernández Robledo G Peter Van Walsum Source Type: research

Streptomyces lividans 66 produces a protease inhibitor via a tRNA-utilizing enzyme interacting with a C-minus NRPS
J Ind Microbiol Biotechnol. 2023 Sep 5:kuad021. doi: 10.1093/jimb/kuad021. Online ahead of print.ABSTRACTSmall peptide aldehydes (SPAs) with protease inhibitory activity are naturally occurring compounds shown to be synthesized by nonribosomal peptide synthetases (NRPS). SPAs are widely used in biotechnology and have been utilized as therapeutic agents. They are also physiologically relevant and have been postulated to regulate the development of their producing microorganisms. Previously, we identified an NRPS-like biosynthetic gene cluster in Streptomyces lividans 66 that lacked a condensation (C) domain but included a t...
Source: Journal of Industrial Microbiology and Biotechnology - September 5, 2023 Category: Microbiology Authors: C ésar Aguilar Karina Verdel-Aranda Hilda E Ramos-Aboites Cuauht émoc Licona-Cassani Francisco Barona-G ómez Source Type: research

Optimizing the strain engineering process for industrial scale production of bio-based molecules
J Ind Microbiol Biotechnol. 2023 Sep 1:kuad025. doi: 10.1093/jimb/kuad025. Online ahead of print.ABSTRACTBiomanufacturing could contribute as much as $30 trillion to the global economy by 2030. But the success of the growing bioeconomy depends on our ability to manufacture high-performing strains in a time- and cost-effective manner. The Design-Build-Test-Learn (DBTL) framework has proven to be an effective strain engineering approach. Significant improvements have been made in genome engineering, genotyping, and phenotyping throughput over the last couple of decades that have greatly accelerated the DBTL cycles. However, ...
Source: Journal of Industrial Microbiology and Biotechnology - September 1, 2023 Category: Microbiology Authors: Eric Abbate Jennifer Andrion Amanda Apel Matthew Biggs Julie Chaves Kristi Cheung Anthony Ciesla Alia Clark-ElSayed Michael Clay Riarose Contridas Richard Fox Glenn Hein Dan Held Andrew Horwitz Stefan Jenkins Karolina Kalbarczyk Nandini Krishnamurthy Mona Source Type: research

Production of S-methyl-methionine using engineered Saccharomyces cerevisiae sake K6
In this study, we attempted to produce it in an engineered microorganism, Saccharomyces cerevisiae, by introducing an MMT gene encoding a Methionine S-methyltransferase from Arabidopsis thaliana. The S. cerevisiae Sake K6 strain which is a Generally Recognized as Safe (GRAS) strain, was chosen as the host because it produces a significant amount of S-adenosylmethionine (SAM), a precursor of SMM. To increase SMM production in the host, MHT1 and SAM4 genes encoding Homocysteine S-methyltransferase were knocked out to prevent SMM degradation. Additionally, MMP1 which encodes S-methyl-methionine permease, was deleted to preven...
Source: Journal of Industrial Microbiology and Biotechnology - August 31, 2023 Category: Microbiology Authors: Jun-Min Lee Min-Ho Park Bu-Soo Park Min-Kyu Oh Source Type: research

Monitoring biofilm growth and dispersal in real-time with impedance biosensors
This study utilized a custom flow cell system with integrated sensors to make real-time impedance measurements of biofilm growth under flow conditions, which were correlated with confocal laser scanning microscopy (CLSM) imaging. Biofilm growth on EIS biosensors in basic aqueous growth media (tryptic soy broth; TSB) and an oil-water emulsion (metalworking fluid; MWF) attenuated in a sigmoidal decay pattern, which lead to an ∼22 to 25% decrease in impedance after 24 hours. Subsequent treatment of established biofilms increased impedance by ∼14% and ∼41% in TSB and MWF, respectively. In the presence of Furanone C-30, a...
Source: Journal of Industrial Microbiology and Biotechnology - August 31, 2023 Category: Microbiology Authors: Matthew McGlennen Markus Dieser Christine M Foreman Stephan Warnat Source Type: research

Diversity and taxonomic distribution of bacterial biosynthetic gene clusters predicted to produce compounds with therapeutically relevant bioactivities
J Ind Microbiol Biotechnol. 2023 Aug 31:kuad024. doi: 10.1093/jimb/kuad024. Online ahead of print.ABSTRACTBacteria have long been a source of natural products with diverse bioactivities that have been developed into therapeutics to treat human disease. Historically, researchers have focused on a few taxa of bacteria, mainly Streptomyces and other actinomycetes. This strategy was initially highly successful and resulted in the golden era of antibiotic discovery. The golden era ended when the most common antibiotics from Streptomyces had been discovered. Rediscovery of known compounds has plagued natural product discovery ev...
Source: Journal of Industrial Microbiology and Biotechnology - August 31, 2023 Category: Microbiology Authors: Max L Beck Siyeon Song Isra E Shuster Aarzu Miharia Allison S Walker Source Type: research

Small Molecule Inducers of Actinobacteria Natural Product Biosynthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad019. doi: 10.1093/jimb/kuad019. Online ahead of print.ABSTRACTActinobacteria are a large and diverse group of bacteria that are known to produce a wide range of secondary metabolites, many of which have important biological activities, including antibiotics, anti-cancer agents, and immunosuppressants. The biosynthesis of these compounds is often highly regulated with many natural products being produced at very low levels in laboratory settings. Environmental factors, such as small molecule elicitors, can induce the production of secondary metabolites. Specifically, they can incr...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Amir Y Alwali Elizabeth I Parkinson Source Type: research