Journal of Industrial Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Cultivation of recombinant Aspergillus niger strains on dairy whey as a carbohydrate source
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae007. doi: 10.1093/jimb/kuae007.ABSTRACTAgricultural waste valorisation provides a sustainable solution to waste management, and combining waste utilisation with commodity production allows for responsible production processes. Recombinant Aspergillus niger D15 strains expressing fungal endoglucanases (Trichoderma reesei eg1 and eg2 and Aspergillus carneus aceg) were evaluated for their ability to utilise lactose as a carbon source to determine whether dairy waste could be used as a feedstock for enzyme production. The recombinant A. niger D15[eg1]PyrG, D15[eg2]PyrG, and D15[ace...
Source: Journal of Industrial Microbiology and Biotechnology - February 1, 2024 Category: Microbiology Authors: Teagan C Crament Kayline Arendsen Shaunita H Rose Trudy Jansen Source Type: research

Transcriptional and translational flux optimization at the key regulatory node for enhanced production of naringenin using acetate in engineered Escherichia coli
In this study, transcriptional and translational regulation of pckA expression at the crucial regulatory node was conducted to optimize naringenin biosynthesis using acetate in E. coli.PMID:38285614 | PMC:PMC10853766 | DOI:10.1093/jimb/kuae006 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - January 29, 2024 Category: Microbiology Authors: Dong H Kim Hyun G Hwang Dae-Yeol Ye Gyoo Y Jung Source Type: research

One-pot chemoenzymatic syntheses of non-canonical amino acids
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae005. doi: 10.1093/jimb/kuae005.ABSTRACTDespite their prevalent use in drug discovery and protein biochemistry, non-canonical amino acids are still challenging to synthesize through purely chemical means. In recent years, biocatalysis has emerged as a transformative paradigm for small-molecule synthesis. One strategy to further empower biocatalysis is to use it in combination with modern chemical reactions and take advantage of the strengths of each method to enable access to challenging structural motifs that were previously unattainable using each method alone. In this Mini-Re...
Source: Journal of Industrial Microbiology and Biotechnology - January 25, 2024 Category: Microbiology Authors: Tsung-Han Chao Xiangyu Wu Hans Renata Source Type: research

Development of platensimycin, platencin, and platensilin overproducers by biosynthetic pathway engineering and fermentation medium optimization
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae003. doi: 10.1093/jimb/kuae003.ABSTRACTThe platensimycin (PTM), platencin (PTN), and platensilin (PTL) family of natural products continues to inspire the discovery of new chemistry, enzymology, and medicine. Engineered production of this emerging family of natural products, however, remains laborious due to the lack of practical systems to manipulate their biosynthesis in the native-producing Streptomyces platensis species. Here we report solving this technology gap by implementing a CRISPR-Cas9 system in S. platensis CB00739 to develop an expedient method to manipulate the PT...
Source: Journal of Industrial Microbiology and Biotechnology - January 23, 2024 Category: Microbiology Authors: Lucas L Fluegel Ming-Rong Deng Ping Su Edward Kalkreuter Dong Yang Jeffrey D Rudolf Liao-Bin Dong Ben Shen Source Type: research

Secretory expression of amylosucrase in Bacillus licheniformis through twin-arginine translocation pathway
This study marks the first successful secretory expression of NpAS in Bacillus species host cells, establishing a foundation for its future efficient production.ONE-SENTENCE SUMMARY: Amylosucrase was secreted in Bacillus licheniformis via the twin-arginine translocation pathway.PMID:38253396 | PMC:PMC10849164 | DOI:10.1093/jimb/kuae004 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - January 22, 2024 Category: Microbiology Authors: Caizhe Wang Dandan Niu Nokuthula Peace Mchunu Meng Zhang Suren Singh Zhengxiang Wang Source Type: research

Correction to: Characterization and catalytic investigation of fungal single-module nonribosomal peptide synthetase in terpene-amino acid meroterpenoid biosynthesis
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae002. doi: 10.1093/jimb/kuae002.NO ABSTRACTPMID:38235611 | PMC:PMC10845890 | DOI:10.1093/jimb/kuae002 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - January 18, 2024 Category: Microbiology Source Type: research

A discussion and evaluation of statistical procedures used by JIMB authors when comparing means
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuae001. doi: 10.1093/jimb/kuae001.ABSTRACTOut of the 166 articles published in Journal of Industrial Microbiology and Biotechnology (JIMB) in 2019-2020 (not including special issues or review articles), 51 of them used a statistical test to compare two or more means. The most popular test was the (Standard) t-test, which often was used to compare several pairs of means. Other statistical procedures used included Fisher's least significant difference (LSD), Tukey's honest significant difference (HSD), and Welch's t-test; and to a lesser extent Bonferroni, Duncan's Multiple Range, S...
Source: Journal of Industrial Microbiology and Biotechnology - January 11, 2024 Category: Microbiology Authors: K Thomas Klasson Source Type: research

Engineered biosynthesis of plant heteroyohimbine and corynantheine alkaloids in Saccharomyces cerevisiae
J Ind Microbiol Biotechnol. 2024 Jan 9;51:kuad047. doi: 10.1093/jimb/kuad047.ABSTRACTMonoterpene indole alkaloids (MIAs) are a class of natural products comprised of thousands of structurally unique bioactive compounds with significant therapeutic values. Due to difficulties associated with isolation from native plant species and organic synthesis of these structurally complex molecules, microbial production of MIAs using engineered hosts are highly desired. In this work, we report the engineering of fully integrated Saccharomyces cerevisiae strains that allow de novo access to strictosidine, the universal precursor to tho...
Source: Journal of Industrial Microbiology and Biotechnology - December 23, 2023 Category: Microbiology Authors: Moriel J Dror Joshua Misa Danielle A Yee Angela M Chu Rachel K Yu Bradley B Chan Lauren S Aoyama Anjali P Chaparala Sarah E O'Connor Yi Tang Source Type: research

Engineered Biosynthesis of Plant Heteroyohimbine and Corynantheine Alkaloids in Saccharomyces cerevisiae
J Ind Microbiol Biotechnol. 2023 Dec 22:kuad047. doi: 10.1093/jimb/kuad047. Online ahead of print.ABSTRACTMonoterpene indole alkaloids (MIAs) are a class of natural products comprised of thousands of structurally unique bioactive compounds with significant therapeutic values. Due to difficulties associated with isolation from native plant species and organic synthesis of these structurally complex molecules, microbial production of MIAs using engineered hosts are highly desired. In this work, we report the engineering of fully integrated Saccharomyces cerevisiae strains that allow de novo access to strictosidine, the unive...
Source: Journal of Industrial Microbiology and Biotechnology - December 23, 2023 Category: Microbiology Authors: Moriel J Dror Joshua Misa Danielle A Yee Angela M Chu Rachel K Yu Bradley B Chan Lauren S Aoyama Anjali P Chaparala Sarah E O'Connor Yi Tang Source Type: research