Recent progress in unraveling the biosynthesis of natural sunscreens mycosporine-like amino acids
J Ind Microbiol Biotechnol. 2023 Nov 9:kuad038. doi: 10.1093/jimb/kuad038. Online ahead of print.ABSTRACTExposure to ultraviolet (UV) rays is a known risk factor for skin cancer, which can be notably mitigated through the application of sun care products. However, escalating concerns regarding the adverse health and environmental impacts of synthetic anti-UV chemicals underscore a pressing need for the development of biodegradable and eco-friendly sunscreen ingredients. Mycosporine-like amino acids (MAAs) represent a family of water-soluble anti-UV natural products synthesized by various organisms. These compounds can prov...
Source: Journal of Industrial Microbiology and Biotechnology - November 11, 2023 Category: Microbiology Authors: Manyun Chen Yujia Jiang Yousong Ding Source Type: research

Antifungal activity and mechanism of action of natural product derivates as potential environmental disinfectants
J Ind Microbiol Biotechnol. 2023 Nov 10:kuad036. doi: 10.1093/jimb/kuad036. Online ahead of print.ABSTRACTThere have been a considerable number of antifungal studies that evaluated natural products, such as medicinal plants, and their secondary metabolites, (phenolic compounds, alkaloids), essential oils, as well as propolis extracts. These studies have investigated natural antifungal substances for use as food preservatives, medicinal agents, or in agriculture as green pesticides because they represent an option of safe, low impact and environmentally friendly antifungal compounds, however, few have studied these natural ...
Source: Journal of Industrial Microbiology and Biotechnology - November 11, 2023 Category: Microbiology Authors: Norma Patricia Silva-Beltr án Stephanie A Boon M Khalid Ijaz Julie McKinney Charles P Gerba Source Type: research

Recent progress in unraveling the biosynthesis of natural sunscreens mycosporine-like amino acids
J Ind Microbiol Biotechnol. 2023 Nov 9:kuad038. doi: 10.1093/jimb/kuad038. Online ahead of print.ABSTRACTExposure to ultraviolet (UV) rays is a known risk factor for skin cancer, which can be notably mitigated through the application of sun care products. However, escalating concerns regarding the adverse health and environmental impacts of synthetic anti-UV chemicals underscore a pressing need for the development of biodegradable and eco-friendly sunscreen ingredients. Mycosporine-like amino acids (MAAs) represent a family of water-soluble anti-UV natural products synthesized by various organisms. These compounds can prov...
Source: Journal of Industrial Microbiology and Biotechnology - November 11, 2023 Category: Microbiology Authors: Manyun Chen Yujia Jiang Yousong Ding Source Type: research

Antifungal activity and mechanism of action of natural product derivates as potential environmental disinfectants
J Ind Microbiol Biotechnol. 2023 Nov 10:kuad036. doi: 10.1093/jimb/kuad036. Online ahead of print.ABSTRACTThere have been a considerable number of antifungal studies that evaluated natural products, such as medicinal plants, and their secondary metabolites, (phenolic compounds, alkaloids), essential oils, as well as propolis extracts. These studies have investigated natural antifungal substances for use as food preservatives, medicinal agents, or in agriculture as green pesticides because they represent an option of safe, low impact and environmentally friendly antifungal compounds, however, few have studied these natural ...
Source: Journal of Industrial Microbiology and Biotechnology - November 11, 2023 Category: Microbiology Authors: Norma Patricia Silva-Beltr án Stephanie A Boon M Khalid Ijaz Julie McKinney Charles P Gerba Source Type: research

Characterization of the off-flavor from Pichia pastoris GS115 during the over-expression of a α-L-rhamnosidase
J Ind Microbiol Biotechnol. 2023 Nov 6:kuad035. doi: 10.1093/jimb/kuad035. Online ahead of print.ABSTRACTThe off-flavor of Pichia pastoris strains is a negative characteristic of proteins overexpressed with this yeast. In the present study, the P. pastoris GS115 overexpressing a α-L-rhamnosidase was taken as the example to characterize the off-flavor via sensory evaluation, gas chromatography-mass spectrometer (GC-MS), gas chromatography-olfaction (GC-O), and omission test. The result showed the off-flavor was attributed to the strong sweaty note, moderate metallic and plastic notes. Four volatile compounds, i.e. tetramet...
Source: Journal of Industrial Microbiology and Biotechnology - November 9, 2023 Category: Microbiology Authors: YuXuan Yao ShengLan Zheng ShiLin Chi Feng Chen Ning Cai ZhenZhen Cai Zhipeng Li Hui Ni Source Type: research

Characterization of the off-flavor from Pichia pastoris GS115 during the over-expression of a α-L-rhamnosidase
J Ind Microbiol Biotechnol. 2023 Nov 6:kuad035. doi: 10.1093/jimb/kuad035. Online ahead of print.ABSTRACTThe off-flavor of Pichia pastoris strains is a negative characteristic of proteins overexpressed with this yeast. In the present study, the P. pastoris GS115 overexpressing a α-L-rhamnosidase was taken as the example to characterize the off-flavor via sensory evaluation, gas chromatography-mass spectrometer (GC-MS), gas chromatography-olfaction (GC-O), and omission test. The result showed the off-flavor was attributed to the strong sweaty note, moderate metallic and plastic notes. Four volatile compounds, i.e. tetramet...
Source: Journal of Industrial Microbiology and Biotechnology - November 9, 2023 Category: Microbiology Authors: YuXuan Yao ShengLan Zheng ShiLin Chi Feng Chen Ning Cai ZhenZhen Cai Zhipeng Li Hui Ni Source Type: research

Characterization of the off-flavor from Pichia pastoris GS115 during the over-expression of a α-L-rhamnosidase
J Ind Microbiol Biotechnol. 2023 Nov 6:kuad035. doi: 10.1093/jimb/kuad035. Online ahead of print.ABSTRACTThe off-flavor of Pichia pastoris strains is a negative characteristic of proteins overexpressed with this yeast. In the present study, the P. pastoris GS115 overexpressing a α-L-rhamnosidase was taken as the example to characterize the off-flavor via sensory evaluation, gas chromatography-mass spectrometer (GC-MS), gas chromatography-olfaction (GC-O), and omission test. The result showed the off-flavor was attributed to the strong sweaty note, moderate metallic and plastic notes. Four volatile compounds, i.e. tetramet...
Source: Journal of Industrial Microbiology and Biotechnology - November 9, 2023 Category: Microbiology Authors: YuXuan Yao ShengLan Zheng ShiLin Chi Feng Chen Ning Cai ZhenZhen Cai Zhipeng Li Hui Ni Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research