Journal of Industrial Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Mechanistic aspects of IPTG (isopropylthio- β-galactoside) transport across the cytoplasmic membrane of Escherichia coli - a rate limiting step in the induction of recombinant protein expression
J Ind Microbiol Biotechnol. 2023 Oct 17:kuad034. doi: 10.1093/jimb/kuad034. Online ahead of print.ABSTRACTCoupling transcription of a cloned gene to the lac operon with induction by isopropylthio-β-galactoside (IPTG) has been a favoured approach for recombinant protein expression using Escherichia coli as a heterologous host for more than six decades. Despite a wealth of experimental data gleaned over this period, a quantitative relationship between extracellular IPTG concentration and consequent levels of recombinant protein expression remains surprisingly elusive across a broad spectrum of experimental conditions. This ...
Source: Journal of Industrial Microbiology and Biotechnology - October 18, 2023 Category: Microbiology Authors: Rodrigo G Simas Adalberto Pessoa Junior Paul F Long Source Type: research

Correction to: Fungi as a commercial source of eumelanin: current understanding & amp; prospects
J Ind Microbiol Biotechnol. 2023 Feb 17;50(1):kuad032. doi: 10.1093/jimb/kuad032.NO ABSTRACTPMID:37824207 | PMC:PMC10569374 | DOI:10.1093/jimb/kuad032 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - October 12, 2023 Category: Microbiology Source Type: research

Diverse Mechanisms of Bioproduction Heterogeneity in Fermentation and Their Control Strategies
J Ind Microbiol Biotechnol. 2023 Oct 3:kuad033. doi: 10.1093/jimb/kuad033. Online ahead of print.ABSTRACTMicrobial bioproduction often faces challenges related to populational heterogeneity, where cells exhibit varying biosynthesis capabilities. Bioproduction heterogeneity can stem from genetic and non-genetic factors, resulting in decreased titer, yield, stability, and reproducibility. Consequently, understanding and controlling bioproduction heterogeneity are crucial for enhancing the economic competitiveness of large-scale biomanufacturing. In this review, we provide a comprehensive overview of current understandings of...
Source: Journal of Industrial Microbiology and Biotechnology - October 4, 2023 Category: Microbiology Authors: Xinyue Mu Fuzhong Zhang Source Type: research