Small Molecule Inducers of Actinobacteria Natural Product Biosynthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad019. doi: 10.1093/jimb/kuad019. Online ahead of print.ABSTRACTActinobacteria are a large and diverse group of bacteria that are known to produce a wide range of secondary metabolites, many of which have important biological activities, including antibiotics, anti-cancer agents, and immunosuppressants. The biosynthesis of these compounds is often highly regulated with many natural products being produced at very low levels in laboratory settings. Environmental factors, such as small molecule elicitors, can induce the production of secondary metabolites. Specifically, they can incr...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Amir Y Alwali Elizabeth I Parkinson Source Type: research

Utilizing a divalent metal ion transporter to control biogenic nanoparticle synthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad020. doi: 10.1093/jimb/kuad020. Online ahead of print.ABSTRACTBiogenic synthesis of inorganic nanomaterials has been demonstrated for both wild and engineered bacterial strains. In many systems the nucleation and growth of nanomaterials is poorly controlled and requires concentrations of heavy metals toxic to living cells. Here we utilized the tools of synthetic biology to engineer a strain of Escherichia coli capable of synthesizing cadmium sulfide nanoparticles from low concentrations of reactants with control over the location of synthesis. Informed by simulations of bacterial...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Manasi Subhash Gangan Kyle L Naughton James Q Boedicker Source Type: research

Small Molecule Inducers of Actinobacteria Natural Product Biosynthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad019. doi: 10.1093/jimb/kuad019. Online ahead of print.ABSTRACTActinobacteria are a large and diverse group of bacteria that are known to produce a wide range of secondary metabolites, many of which have important biological activities, including antibiotics, anti-cancer agents, and immunosuppressants. The biosynthesis of these compounds is often highly regulated with many natural products being produced at very low levels in laboratory settings. Environmental factors, such as small molecule elicitors, can induce the production of secondary metabolites. Specifically, they can incr...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Amir Y Alwali Elizabeth I Parkinson Source Type: research

Utilizing a divalent metal ion transporter to control biogenic nanoparticle synthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad020. doi: 10.1093/jimb/kuad020. Online ahead of print.ABSTRACTBiogenic synthesis of inorganic nanomaterials has been demonstrated for both wild and engineered bacterial strains. In many systems the nucleation and growth of nanomaterials is poorly controlled and requires concentrations of heavy metals toxic to living cells. Here we utilized the tools of synthetic biology to engineer a strain of Escherichia coli capable of synthesizing cadmium sulfide nanoparticles from low concentrations of reactants with control over the location of synthesis. Informed by simulations of bacterial...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Manasi Subhash Gangan Kyle L Naughton James Q Boedicker Source Type: research

Small Molecule Inducers of Actinobacteria Natural Product Biosynthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad019. doi: 10.1093/jimb/kuad019. Online ahead of print.ABSTRACTActinobacteria are a large and diverse group of bacteria that are known to produce a wide range of secondary metabolites, many of which have important biological activities, including antibiotics, anti-cancer agents, and immunosuppressants. The biosynthesis of these compounds is often highly regulated with many natural products being produced at very low levels in laboratory settings. Environmental factors, such as small molecule elicitors, can induce the production of secondary metabolites. Specifically, they can incr...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Amir Y Alwali Elizabeth I Parkinson Source Type: research

Utilizing a divalent metal ion transporter to control biogenic nanoparticle synthesis
J Ind Microbiol Biotechnol. 2023 Aug 16:kuad020. doi: 10.1093/jimb/kuad020. Online ahead of print.ABSTRACTBiogenic synthesis of inorganic nanomaterials has been demonstrated for both wild and engineered bacterial strains. In many systems the nucleation and growth of nanomaterials is poorly controlled and requires concentrations of heavy metals toxic to living cells. Here we utilized the tools of synthetic biology to engineer a strain of Escherichia coli capable of synthesizing cadmium sulfide nanoparticles from low concentrations of reactants with control over the location of synthesis. Informed by simulations of bacterial...
Source: Journal of Industrial Microbiology and Biotechnology - August 16, 2023 Category: Microbiology Authors: Manasi Subhash Gangan Kyle L Naughton James Q Boedicker Source Type: research

Transcriptomics analysis and fed-batch regulation of high astaxanthin producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution
This study provides technical basis and theoretical research for promoting industrialization of astaxanthin production of P. rhodozyma.PMID:37580133 | DOI:10.1093/jimb/kuad015 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - August 14, 2023 Category: Microbiology Authors: Liang Yang Hao-Yi Yang Li You Hui Ni Ze-Dong Jiang Xi-Ping Du Yan-Bing Zhu Ming-Jing Zheng Li-Jun Li Rui Lin Zhi-Peng Li Qing-Biao Li Source Type: research

Transcriptomics analysis and fed-batch regulation of high astaxanthin producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution
This study provides technical basis and theoretical research for promoting industrialization of astaxanthin production of P. rhodozyma.PMID:37580133 | DOI:10.1093/jimb/kuad015 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - August 14, 2023 Category: Microbiology Authors: Liang Yang Hao-Yi Yang Li You Hui Ni Ze-Dong Jiang Xi-Ping Du Yan-Bing Zhu Ming-Jing Zheng Li-Jun Li Rui Lin Zhi-Peng Li Qing-Biao Li Source Type: research

Transcriptomics analysis and fed-batch regulation of high astaxanthin producing Phaffia rhodozyma/Xanthophyllomyces dendrorhous obtained through adaptive laboratory evolution
This study provides technical basis and theoretical research for promoting industrialization of astaxanthin production of P. rhodozyma.PMID:37580133 | DOI:10.1093/jimb/kuad015 (Source: Journal of Industrial Microbiology and Biotechnology)
Source: Journal of Industrial Microbiology and Biotechnology - August 14, 2023 Category: Microbiology Authors: Liang Yang Hao-Yi Yang Li You Hui Ni Ze-Dong Jiang Xi-Ping Du Yan-Bing Zhu Ming-Jing Zheng Li-Jun Li Rui Lin Zhi-Peng Li Qing-Biao Li Source Type: research

A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1
J Ind Microbiol Biotechnol. 2023 Aug 3:kuad018. doi: 10.1093/jimb/kuad018. Online ahead of print.ABSTRACTShewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances t...
Source: Journal of Industrial Microbiology and Biotechnology - August 3, 2023 Category: Microbiology Authors: Megan C Gruenberg Michaela A TerAvest Source Type: research

A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1
J Ind Microbiol Biotechnol. 2023 Aug 3:kuad018. doi: 10.1093/jimb/kuad018. Online ahead of print.ABSTRACTShewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances t...
Source: Journal of Industrial Microbiology and Biotechnology - August 3, 2023 Category: Microbiology Authors: Megan C Gruenberg Michaela A TerAvest Source Type: research

A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1
J Ind Microbiol Biotechnol. 2023 Aug 3:kuad018. doi: 10.1093/jimb/kuad018. Online ahead of print.ABSTRACTShewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances t...
Source: Journal of Industrial Microbiology and Biotechnology - August 3, 2023 Category: Microbiology Authors: Megan C Gruenberg Michaela A TerAvest Source Type: research

A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1
J Ind Microbiol Biotechnol. 2023 Aug 3:kuad018. doi: 10.1093/jimb/kuad018. Online ahead of print.ABSTRACTShewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances t...
Source: Journal of Industrial Microbiology and Biotechnology - August 3, 2023 Category: Microbiology Authors: Megan C Gruenberg Michaela A TerAvest Source Type: research

A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1
J Ind Microbiol Biotechnol. 2023 Aug 3:kuad018. doi: 10.1093/jimb/kuad018. Online ahead of print.ABSTRACTShewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances t...
Source: Journal of Industrial Microbiology and Biotechnology - August 3, 2023 Category: Microbiology Authors: Megan C Gruenberg Michaela A TerAvest Source Type: research

A common inducer molecule enhances sugar utilization by Shewanella oneidensis MR-1
J Ind Microbiol Biotechnol. 2023 Aug 3:kuad018. doi: 10.1093/jimb/kuad018. Online ahead of print.ABSTRACTShewanella oneidensis MR-1 is an electroactive bacterium that is a promising host for bioelectrochemical technologies, which makes it a common target for genetic engineering, including gene deletions and expression of heterologous pathways. Expression of heterologous genes and gene knockdown via CRISPRi in S. oneidensis are both frequently induced by β-D-1-thiogalactopyranoside (IPTG), a commonly used inducer molecule across many model organisms. Here, we report and characterize an unexpected phenotype; IPTG enhances t...
Source: Journal of Industrial Microbiology and Biotechnology - August 3, 2023 Category: Microbiology Authors: Megan C Gruenberg Michaela A TerAvest Source Type: research