Journal of Industrial Microbiology and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Determining mating type and ploidy in Rhodotorula toruloides and its effect on growth on sugars from lignocellulosic biomass
J Ind Microbiol Biotechnol. 2023 Nov 21:kuad040. doi: 10.1093/jimb/kuad040. Online ahead of print.ABSTRACTRhodotorula toruloides is being developed for use in industrial biotechnology processes because of its favorable physiology. This includes its ability to produce and store large amounts of lipids in the form of intracellular lipid bodies. Nineteen strains were characterized for mating type, ploidy, robustness for growth, and accumulation of lipids on inhibitory switchgrass hydrolysate (SGH). Mating type was determined using a novel PCR-based assay, which was validated using the classical microscopic test. Three of the ...
Source: Journal of Industrial Microbiology and Biotechnology - November 21, 2023 Category: Microbiology Authors: Daiane Dias Lopes Bruce S Dien Ronald E Hector Vijay Singh Stephanie R Thompson Patricia J Slininger Kyria Boundy-Mills Sujit S Jagtap Christopher V Rao Source Type: research

Genomic, phenotypic and clinical safety of Limosilactobacillus reuteri ATCC PTA 4659
In this study, we have performed a systematic safety assessment of Limosilactobacillus reuteri ATCC PTA 4659 based on genome analysis, antibiotic susceptibility testing, phenotypic characterization, and a human clinical safety study. Genome sequence analysis showed that the strain is free from virulence and antibiotic resistance genes. Connected to this, phenotypic characterization showed that the strain is susceptible to the main classes of antibiotics. L. reuteri ATCC PTA 4659 was shown to produce histamine, which has previously been described as an anti-inflammatory mediator produced by certain L. reuteri strains. Howev...
Source: Journal of Industrial Microbiology and Biotechnology - November 17, 2023 Category: Microbiology Authors: Malin Sendelius Jakob Axelsson Peidi Liu Stefan Roos Source Type: research

Improved 13C metabolic flux analysis in Escherichia coli metabolism: application of a high resolution MS (GC-EI-QTOF) for comprehensive assessment MS/MS fragments
J Ind Microbiol Biotechnol. 2023 Nov 13:kuad039. doi: 10.1093/jimb/kuad039. Online ahead of print.ABSTRACTGas chromatography-tandem mass spectrometry with electron ionization (GC-EI-MS/MS) provides rich information on stable-isotope labeling for 13C-metabolic flux analysis (13C-MFA). To pave the way for the routine application of tandem MS data for metabolic flux quantification, we aimed to compile a comprehensive library of GC-EI-MS/MS fragments of tert-butyldimethylsilyl (TBDMS) derivatized proteinogenic amino acids. First, we established an analytical workflow which combines high resolution gas chromatography-quadrupole...
Source: Journal of Industrial Microbiology and Biotechnology - November 14, 2023 Category: Microbiology Authors: Chris Richter Eva Grafahrend-Belau J örg Ziegler Manish L Raorane Bj örn H Junker Source Type: research