Lessons from assembling a microbial natural product and pre-fractionated extract library in an academic laboratory
J Ind Microbiol Biotechnol. 2023 Dec 5:kuad042. doi: 10.1093/jimb/kuad042. Online ahead of print.ABSTRACTMicrobial natural products are specialized metabolites that are sources of many bioactive compounds including antibiotics, antifungals, antiparasitics, anticancer agents, and probes of biology. The assembly of libraries of producers of natural products has traditionally been the province of the pharmaceutical industry. This sector has gathered significant historical collections of bacteria and fungi to identify new drug leads with outstanding outcomes-upwards of 60% of drug scaffolds originate from such libraries. Despite this success, the repeated rediscovery of known compounds and the resultant diminishing chemical novelty contributed to a pivot from this source of bioactive compounds toward more tractable synthetic compounds in the drug industry. The advent of advanced mass spectrometry tools, along with rapid whole genome sequencing and in silico identification of biosynthetic gene clusters that encode the machinery necessary for the synthesis of specialized metabolites, offers the opportunity to revisit microbial natural product libraries with renewed vigor. Assembling a suitable library of microbes and extracts for screening requires the investment of resources and the development of methods that have customarily been the proprietary purview of large pharmaceutical companies. Here, we report a perspective on our efforts to assemble a library of natural product-produc...
Source: Journal of Industrial Microbiology and Biotechnology - Category: Microbiology Authors: Michael A Cook Daniel Pallant Linda Ejim Arlene D Sutherland Xiaodong Wang Jarrod W Johnson Susan McCusker Xuefei Chen Maya George Sommer Chou Kalinka Koteva Wenliang Wang Christian Hobson Dirk Hackenberger Nicholas Waglechner Obie Ejim Tracey Campbell Ri Source Type: research
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