Reconstructed human epidermis ‐based testing strategy of skin sensitization potential and potency classification using epidermal sensitization assay and in silico data
AbstractThe hazards and potency of skin sensitizers are traditionally determined using animal tests such as the local lymph node assay (LLNA); however, significant progress has been made in the development of non-animal test methods addressing the first three mechanistic key events of adverse outcome pathway in skin sensitization. We developed the epidermal sensitization assay (EpiSensA), which is a reconstructed human epidermis-based assay, by measuring four genes related to critical keratinocyte responses during skin sensitization. Four in vitro skin sensitization test methods (EpiSensA, direct peptide reactivity assay [...
Source: Journal of Applied Toxicology - October 18, 2023 Category: Toxicology Authors: Hideyuki Mizumachi,
Sho Suzuki,
Megumi Sakuma,
Midori Natsui,
Noriyasu Imai,
Masaaki Miyazawa Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - October 16, 2023 Category: Toxicology Tags: ISSUE INFORMATION Source Type: research
Genotoxicity of bisphenol AF in rats: Detrimental to male reproductive system and probable stronger micronucleus induction potency than BPA
AbstractBisphenol AF (BPAF), as one of structural analogs of BPA, has been increasingly used in recent years. However, limited studies have suggested its adverse effects similar to or higher than BPA. In order to explore the general toxicity and genotoxicity of subacute exposure to BPAF, the novel 28-day multi-endpoint (Pig-a assay + micronucleus [MN] test + comet assay) genotoxicity evaluation platform was applied. Male rats were randomly distributed into seven main experimental groups and four satellite groups. The main experimental groups included BPAF-treated groups (0.5, 5, and 50 μg/kg·bw/d), BPA group (...
Source: Journal of Applied Toxicology - October 15, 2023 Category: Toxicology Authors: Xia Zhu,
Li Cao,
Yufei Liu,
Xinyao Tang,
Yeqiu Miao,
Jing Zhang,
Lishi Zhang,
Zhenchao Jia,
Jinyao Chen Tags: RESEARCH ARTICLE Source Type: research
Lowering the pH leads to the disaggregation of NiO and ZnO nanoparticles and modifies the mutagenic response
AbstractIn a changing environmental scenario, acid rain can have a significant impact on aquatic ecosystems. Acidification is known to produce corrosion in metals, hence increasing their harmful effects on the environment, organisms and human health. The prevalent use of metallic nanoparticles (NPs) in everyday products raises concerns regarding exposure and nanotoxicity even in these acidified conditions. We thus report on the cytotoxic and genotoxic potential of nickel oxide (NiO-NP) and zinc oxide (ZnO-NP) NPs when suspended in aqueous media in light of pH variations (7.5 and 5). A modified microsuspension method of the...
Source: Journal of Applied Toxicology - October 13, 2023 Category: Toxicology Authors: Ismael Kr üger Pescke,
Lívia de Oliveira Rozino,
Karoline Zenato,
Tatiane Cardozo,
Wladimir Hernandez Flores,
Vera Maria Ferrão Vargas Tags: RESEARCH ARTICLE Source Type: research
Species differences in small intestinal exposure ‐related epithelial vacuolation in rats and dogs treated with a heteroaryldihydropyrimidine molecule
In conclusion, HAP-1-induced intestinal epithelial vacuolation was related to lipid metabolism dysregulation in both species and ER-related injuries in rats only. The species differences were likely related to the difference in intestinal exposure to HAP-1 and its reactive metabolite. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - October 10, 2023 Category: Toxicology Authors: Jieyu Dai,
Tao Chen,
Ryan Meng,
Ferran Jardi,
Stephanie Kourula,
Ly Pham,
Sandra De Jonghe,
Ann De Smedt,
Anna ‐Lena Frisk,
Jianxun Xie Tags: RESEARCH ARTICLE Source Type: research
Inhibition of voltage ‐dependent K+ channels in rabbit coronary arterial smooth muscle cells by the atypical antipsychotic agent sertindole
In this study, native VSMCs from rabbit coronary arteries were used to investigate the inhibitory effect of sertindole, an atypical antipsychotic agent, on Kv channels. Sertindole induced dose-dependent inhibition of Kv channels, with an IC50 of 3.13 ± 0.72 μM. Although sertindole did not cause a change in the steady-state activation curve, it did lead to a negative shift in the steady-state inactivation curve. The application of 1- or 2-Hz train pulses failed to alter the sertindole-induced inhibition of Kv channels, suggesting use-ind ependent effects of the drug. The inhibitory response to sertindole was signif...
Source: Journal of Applied Toxicology - October 9, 2023 Category: Toxicology Authors: Wenwen Zhuang,
Seo ‐Yeong Mun,
Minju Park,
Junsu Jeong,
Hye Ryung Kim,
Sunghun Na,
Se Jin Lee,
Hongzoo Park,
Won Sun Park Tags: RESEARCH ARTICLE Source Type: research
The repeated administration of rhIL ‐12 for 14 weeks in rhesus monkeys: A toxicity assessment
In this study, we conducted a comprehensive evaluation of the toxicity and safety of repeated rhIL-12 (recombinant human interleukin-12) administration in rhesus monkeys by assessing its effects on the immune system, organ function, and vital signs. Rhesus monkeys were subcutaneously injected with 0.5, 2.5, and 12.5 μg/kg of rhIL-12 for up to for 14 consecutive weeks. The low dose exhibited no signs of toxicity, whereas animals receiving higher doses displayed symptoms such as loose stools, reduced activity, anemia, and elevated liver function indicators (AST and TBIL). Following three administrations of 12. 5 μg/kg, ...
Source: Journal of Applied Toxicology - September 29, 2023 Category: Toxicology Authors: Huiqin Ding,
Huan Qin,
Jiangang Wang,
Yansheng Dong,
Quanjun Wang,
Yantao Han Tags: RESEARCH ARTICLE Source Type: research
Dual effects of endogenous formaldehyde on the organism and drugs for its removal
This study offers fresh perspectives for managing illnesses associated with endogenous FA exposure. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - September 28, 2023 Category: Toxicology Authors: Jiaxin Chen,
Wenhui Chen,
Jinjia Zhang,
Huanhuan Zhao,
Ji Cui,
Junzi Wu,
Anhua Shi Tags: REVIEW ARTICLE Source Type: research
