Petite Integration Factor 1 knockdown enhances gemcitabine sensitivity in pancreatic cancer cells via increasing DNA damage
In conclusion, PIF1 silencing enhanced gemcitabine sensitivity resp onse to pancreatic cancer cells through aggrandizing DNA damage. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - May 17, 2023 Category: Toxicology Authors: Kun Wang,
Xiangdong Hua,
Xibo Fu,
Zhiqiang Hao,
Ao Jiao,
Siyuan Li Tags: RESEARCH ARTICLE Source Type: research
Metabolism and elimination kinetics of mono ‐hydroxylated PAHs metabolites (OH‐PAHs) following single exposure to different combinations of PAH4 in rats
AbstractThe metabolism of polycyclic aromatic hydrocarbons (PAHs) and the elimination kinetics of their mono-hydroxylated metabolites (OH-PAHs) following single exposure to different combinations of four PAHs (PAH4) were studied. Male Sprague –Dawley rats were orally exposed to a single dose of benzo[a]pyrene (B[a]P) or PAH2 (B[a]P + chrysene), PAH3 (B[a]P + chrysene + benz[a]anthracene (B[a]A)) and PAH4 (B[a]P + chrysene + B[a]A + benzo[b]fluoranthene (B[b]F)) with each combination adjusted to the same dose of individual compound. OH- PAHs including 3-OHB[a]P, 3-hydroxychrysene (3-OHC), 3-OHB[a]A and 1-hydroxypyrene (1-...
Source: Journal of Applied Toxicology - May 16, 2023 Category: Toxicology Authors: Miao Yang,
Kanmin Mao,
Xin Cao,
Hongjuan Liu,
Xinzheng Wang,
Weifeng Mao,
Liping Hao Tags: RESEARCH ARTICLE Source Type: research
Petite integration factor 1 knockdown enhances gemcitabine sensitivity in pancreatic cancer cells via increasing DNA damage
In conclusion, PIF1 silencing enhanced gemcitabine sensitivity respons e to pancreatic cancer cells through aggrandizing DNA damage. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - May 15, 2023 Category: Toxicology Authors: Kun Wang,
Xiangdong Hua,
Xibo Fu,
Zhiqiang Hao,
Ao Jiao,
Siyuan Li Tags: RESEARCH ARTICLE Source Type: research
Exogenous IL ‐10 post‐treatment along with TLR4 and TNFR1 blockade improves tissue antioxidant status by modulating sepsis induced macrophage polarization
AbstractMulti-organ dysfunction is one of the major reasons behind the high mortality of sepsis throughout the world. With the pathophysiology of sepsis remaining largely unknown, the uncontrolled ROS production along with the decreased anti-oxidants contributes to the progression toward septic shock. Being the effector cells of the innate immunity system, macrophages secrete both pro- as well as anti-inflammatory mediators during inflammation. LPS binding to TLR4 releases TNF- α which initiates pro-inflammatory events through TNFR1 signaling. However, it is counteracted by the anti-inflammatory IL-10 causing decreased ox...
Source: Journal of Applied Toxicology - May 13, 2023 Category: Toxicology Authors: Ritasha Sawoo,
Rajen Dey,
Rituparna Ghosh,
Biswadev Bishayi Tags: RESEARCH ARTICLE Source Type: research
CDCT ‐induced nephrotoxicity in rat by apoptosis via metabolic disturbance
This study aims to investigate the CDCT-induced changes of morphology in kidney and metabolites and further explore the possible mechanisms of CDCT-induced nephrotoxicity.Sprague –Dawley rats were exposed to the CDCT at a clinical equivalent dose for 6 days. CDCT exposure can induce kidney injury and death. Pathological changes, including creatinine, urea nitrogen, and histopathology, were observed in rats. Furthermore, metabolomic-driven energy and glycerophospholipid metabolism pathway disorders, accompanied by remarkably changed key metabolit es, such as succinate, leukotriene B4 (LTB4), and cardiolipin (CL), are ob...
Source: Journal of Applied Toxicology - May 11, 2023 Category: Toxicology Authors: Chijing Zuo,
Yan Liu,
Jie Wang,
Weidong Yu,
Yujie Liu,
Yanyan Zhang,
Jingjing Xu,
Daiyin Peng,
Can Peng Tags: RESEARCH ARTICLE Source Type: research
Characteristics of transcription profile, adhesion and migration of SETD2 ‐loss Met‐5A mesothelial cells exposed with crocidolite
AbstractAsbestos is a fibrous silicate mineral exhibiting biopersistence and carcinogenic properties and contributes to mesothelioma. Despite the concept of gene-environmental interaction in pathogenesis of mesothelioma, the possible pathophysiological changes of mesothelial cells simultaneously with SET domain containing 2 (SETD2) loss and asbestos exposure remains obscure. Herein, CRISPR/Cas9-mediated SETD2 knockout Met-5A mesothelial cells (Met-5ASETD2-KO) were established and exposed with crocidolite, an amphibole asbestos. Cell viability of Met-5ASETD2-KO appeared to dramatically decrease with ≥2.5 μg/cm2 crocidol...
Source: Journal of Applied Toxicology - May 11, 2023 Category: Toxicology Authors: Dan Yang,
Chiyun Chen,
Hailing Xia,
Junqiang Chen,
Min Yu Tags: RESEARCH ARTICLE Source Type: research
Predicting binding between 55 cannabinoids and 4,799 biological targets by in silico methods
AbstractRecently, there has been an increase in cannabis-derived products being marketed as foods, dietary supplements, and other consumer products. Cannabis contains over a hundred cannabinoids, many of which have unknown physiological effects. Since there are large numbers of cannabinoids, and many are not commercially available for in vitro testing, an in silico tool (Chemotargets Clarity software) was used to predict binding between 55 cannabinoids and 4,799 biological targets (enzymes, ion channels, receptors, and transporters). This tool relied on quantitative structure activity relationships (QSAR), structural simil...
Source: Journal of Applied Toxicology - May 11, 2023 Category: Toxicology Authors: Michael F. Santillo,
Robert L. Sprando Tags: RESEARCH ARTICLE Source Type: research
Identification of genes showing altered DNA methylation and gene expression in the renal proximal tubular cells of rats treated with ochratoxin A for 13 weeks
In conclusion, a variety of genes engaged in carcinogenic processes were obtained by epigenetic gene profiling in rat renal tubular cells specific to OTA treatment for 13 weeks. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - May 10, 2023 Category: Toxicology Authors: Shunsuke Ozawa,
Ryota Ojiro,
Qian Tang,
Xinyu Zou,
Gye ‐Hyeong Woo,
Toshinori Yoshida,
Makoto Shibutani Tags: RESEARCH ARTICLE Source Type: research
Evaluation of in vitro cytotoxic and genotoxic effects of the 3 ‐(3,4‐dihydroxyphenyl)‐8‐hydroxycoumarin
AbstractA wide variety of natural and synthetic coumarins present therapeutic potential. Therefore, the assessment of their safety for humans is essential. 3-(3,4-Dihydroxyphenyl)-8-hydroxycoumarin is a coumarin derivative with antioxidant properties, among other biological activities. The aim of this study is to evaluate the cytotoxic and genotoxic potential of this molecule on peripheral blood mononuclear cells (PBMC) and human hepatocellular carcinoma cells (HepG2/C3A). The results obtained for the cytotoxicity assays, evaluated by the trypan blue staining assay, using concentrations between 0.1 and 20 μg/mL, showed...
Source: Journal of Applied Toxicology - May 9, 2023 Category: Toxicology Authors: Edvannia Santos Silva,
Maria Jo ão Matos,
Edson Luis Maistro Tags: RESEARCH ARTICLE Source Type: research
Issue Information
No abstract is available for this article. (Source: Journal of Applied Toxicology)
Source: Journal of Applied Toxicology - May 8, 2023 Category: Toxicology Tags: ISSUE INFORMATION Source Type: research
In silico prediction of hERG blockers using machine learning and deep learning approaches
In this study, a dataset containing 4556 compounds with IC50 values determined by patch clamp techniques on mammalian lineage cells was collected, and hERG blockers and non-blockers were distinguished according to three single thresholds and two binary thresholds. Four machine learning (ML) algorithms combining four molecular fingerprints and molecular descriptors as well as graph convolutional neural networks (GCNs) were used to construct a series of binary classification models. The results showed that the best models varied for different thresholds. The ML models implemented by support vector machine and random forest p...
Source: Journal of Applied Toxicology - May 6, 2023 Category: Toxicology Authors: Yuanting Chen,
Xinxin Yu,
Weihua Li,
Yun Tang,
Guixia Liu Tags: RESEARCH ARTICLE Source Type: research
Characteristics of transcription profile, adhesion and migration of SETD2 ‐loss Met‐5A mesothelial cells exposed with crocidolite
AbstractAsbestos is fibrous silicate mineral exhibiting biopersistence and carcinogen property and contribute to mesothelioma. Despite the concept of gene-environmental interaction in pathogenesis of mesothelioma, the possible pathophysiological changes of mesothelial cells simultaneously with SETD2 loss and asbestos exposure remains obscure. Herein, CRISPR/Cas9-mediated SETD2 knockout Met-5A mesothelial cells (Met-5ASETD2-KO) were established and exposed with crocidolite, an amphibole asbestos. Cell viability of Met-5ASETD2-KO appeared to dramatically decrease with ≥ 2.5 μg/cm2 crocidolite exposure as compared to Met-5...
Source: Journal of Applied Toxicology - May 6, 2023 Category: Toxicology Authors: Dan Yang,
Chiyun Chen,
Hailing Xia,
Junqiang Chen,
Min Yu Tags: RESEARCH ARTICLE Source Type: research
Magnetized vermiculite as a tool for the treatment of produced water generated by oil companies: Effects on aquatic organisms before and after treatment
AbstractProduced water (PW) generated by oil companies is a highly impacting waste that contains chemicals such as metals and organic and inorganic compounds. Given its polluting potential, PW requires effective treatment before being discharged into the environment. Conventional treatments have limited efficiency in removing PW toxicity, so alternative approaches must be developed and standardized. In this context, treatment with adsorbent materials like magnetized vermiculite (VMT-mag) is highlighted. This work aimed to evaluate the efficiency of treatment with VMT-mag in reducing PW toxicity to aquatic biota. For this p...
Source: Journal of Applied Toxicology - May 4, 2023 Category: Toxicology Authors: Tais S. Viana,
Diana Campos,
Mariana Bartilotti,
Fernanda G. Leite,
Maria Valnice Zanoni,
Daniel J. Dorta,
Danielle P. Oliveira,
Jo ão L. T. Pestana Tags: RESEARCH ARTICLE Source Type: research
