Oat species and interspecific amphiploids show predominance of diploid nuclei in the syncytial endosperm
This study showed, for the first time, the dominance of diploid nuclei in the syncytial stage of the central cell of embryo sac in oat species and amphiploids. The dominance of diploid nuclei, which were the basis for the formation of polyploid nuclei, was weaker in amphiploids due to aneuploid events. The genomic in situ hybridisation method applied in the study did not distinguish the maternal and paternal haploid nuclei of embryo sac. However, this method demonstrated the lack of a set of genomes of one haploid nucleus. Embryological analyses of the initial stages of oat endosperm development revealed a fertilised egg c...
Source: J Appl Genet - November 7, 2023 Category: Genetics & Stem Cells Authors: Paulina Tomaszewska Romuald Kosina Source Type: research
Oat species and interspecific amphiploids show predominance of diploid nuclei in the syncytial endosperm
This study showed, for the first time, the dominance of diploid nuclei in the syncytial stage of the central cell of embryo sac in oat species and amphiploids. The dominance of diploid nuclei, which were the basis for the formation of polyploid nuclei, was weaker in amphiploids due to aneuploid events. The genomic in situ hybridisation method applied in the study did not distinguish the maternal and paternal haploid nuclei of embryo sac. However, this method demonstrated the lack of a set of genomes of one haploid nucleus. Embryological analyses of the initial stages of oat endosperm development revealed a fertilised egg c...
Source: J Appl Genet - November 7, 2023 Category: Genetics & Stem Cells Authors: Paulina Tomaszewska Romuald Kosina Source Type: research
Oat species and interspecific amphiploids show predominance of diploid nuclei in the syncytial endosperm
This study showed, for the first time, the dominance of diploid nuclei in the syncytial stage of the central cell of embryo sac in oat species and amphiploids. The dominance of diploid nuclei, which were the basis for the formation of polyploid nuclei, was weaker in amphiploids due to aneuploid events. The genomic in situ hybridisation method applied in the study did not distinguish the maternal and paternal haploid nuclei of embryo sac. However, this method demonstrated the lack of a set of genomes of one haploid nucleus. Embryological analyses of the initial stages of oat endosperm development revealed a fertilised egg c...
Source: J Appl Genet - November 7, 2023 Category: Genetics & Stem Cells Authors: Paulina Tomaszewska Romuald Kosina Source Type: research
Combining MTI-31 with RAD001 inhibits tumor growth and invasion of kidney cancer by activating autophagy
In this study, CCK8, colony formation assays, and ethynyl deoxyuridine (EdU) analysis were conducted to detect cell proliferation. Furthermore, Transwell assays were also conducted for cell migration analysis. In addition to these, the researchers also performed the flow cytometry process to identify the cells that are undergoing apoptosis. In vivo, experiments were conducted to measure the growth of tumors and metastasis. In this study, the treatment provided through a combination of MTI-31 and RAD001 significantly inhibited the kidney cancer cells' proliferation and tumor growth. Furthermore, there was a notable reductio...
Source: J Appl Genet - November 6, 2023 Category: Genetics & Stem Cells Authors: Wenye Zhang Chen Yang Lujia Zou Yiwen Zang Jimeng Hu Yun Hu Chenyang Xu Rongzong Liu Hao Wang Zuquan Xiong Source Type: research
Inhibition of the glutamate-cysteine ligase catalytic subunit with buthionine sulfoximine enhances the cytotoxic effect of doxorubicin and cyclophosphamide in Burkitt lymphoma cells
J Appl Genet. 2023 Nov 2. doi: 10.1007/s13353-023-00797-1. Online ahead of print.ABSTRACTBurkitt lymphoma (BL) is a highly aggressive lymphoma that mainly affects children and young adults. Chemotherapy is effective in young BL patients but the outcome in adults is less satisfactory. Therefore, there is a need to enhance the cytotoxic effect of drugs used in BL treatment. Glutathione (GSH) is an important antioxidant involved in processes such as regulation of oxidative stress and drug detoxification. Elevated GSH levels have been observed in many cancers and were associated with chemoresistance. We previously identified G...
Source: J Appl Genet - November 2, 2023 Category: Genetics & Stem Cells Authors: Marta Kazimierska Aleksandra Le śniewska Anja Bakker Arjan Diepstra Marta El żbieta Kasprzyk Marta Podralska Karolina Rassek Joost Kluiver Anke van den Berg Natalia Rozwadowska Agnieszka Dzikiewicz-Krawczyk Source Type: research
Inhibition of the glutamate-cysteine ligase catalytic subunit with buthionine sulfoximine enhances the cytotoxic effect of doxorubicin and cyclophosphamide in Burkitt lymphoma cells
J Appl Genet. 2023 Nov 2. doi: 10.1007/s13353-023-00797-1. Online ahead of print.ABSTRACTBurkitt lymphoma (BL) is a highly aggressive lymphoma that mainly affects children and young adults. Chemotherapy is effective in young BL patients but the outcome in adults is less satisfactory. Therefore, there is a need to enhance the cytotoxic effect of drugs used in BL treatment. Glutathione (GSH) is an important antioxidant involved in processes such as regulation of oxidative stress and drug detoxification. Elevated GSH levels have been observed in many cancers and were associated with chemoresistance. We previously identified G...
Source: J Appl Genet - November 2, 2023 Category: Genetics & Stem Cells Authors: Marta Kazimierska Aleksandra Le śniewska Anja Bakker Arjan Diepstra Marta El żbieta Kasprzyk Marta Podralska Karolina Rassek Joost Kluiver Anke van den Berg Natalia Rozwadowska Agnieszka Dzikiewicz-Krawczyk Source Type: research
Inhibition of the glutamate-cysteine ligase catalytic subunit with buthionine sulfoximine enhances the cytotoxic effect of doxorubicin and cyclophosphamide in Burkitt lymphoma cells
J Appl Genet. 2023 Nov 2. doi: 10.1007/s13353-023-00797-1. Online ahead of print.ABSTRACTBurkitt lymphoma (BL) is a highly aggressive lymphoma that mainly affects children and young adults. Chemotherapy is effective in young BL patients but the outcome in adults is less satisfactory. Therefore, there is a need to enhance the cytotoxic effect of drugs used in BL treatment. Glutathione (GSH) is an important antioxidant involved in processes such as regulation of oxidative stress and drug detoxification. Elevated GSH levels have been observed in many cancers and were associated with chemoresistance. We previously identified G...
Source: J Appl Genet - November 2, 2023 Category: Genetics & Stem Cells Authors: Marta Kazimierska Aleksandra Le śniewska Anja Bakker Arjan Diepstra Marta El żbieta Kasprzyk Marta Podralska Karolina Rassek Joost Kluiver Anke van den Berg Natalia Rozwadowska Agnieszka Dzikiewicz-Krawczyk Source Type: research
Inhibition of the glutamate-cysteine ligase catalytic subunit with buthionine sulfoximine enhances the cytotoxic effect of doxorubicin and cyclophosphamide in Burkitt lymphoma cells
J Appl Genet. 2023 Nov 2. doi: 10.1007/s13353-023-00797-1. Online ahead of print.ABSTRACTBurkitt lymphoma (BL) is a highly aggressive lymphoma that mainly affects children and young adults. Chemotherapy is effective in young BL patients but the outcome in adults is less satisfactory. Therefore, there is a need to enhance the cytotoxic effect of drugs used in BL treatment. Glutathione (GSH) is an important antioxidant involved in processes such as regulation of oxidative stress and drug detoxification. Elevated GSH levels have been observed in many cancers and were associated with chemoresistance. We previously identified G...
Source: J Appl Genet - November 2, 2023 Category: Genetics & Stem Cells Authors: Marta Kazimierska Aleksandra Le śniewska Anja Bakker Arjan Diepstra Marta El żbieta Kasprzyk Marta Podralska Karolina Rassek Joost Kluiver Anke van den Berg Natalia Rozwadowska Agnieszka Dzikiewicz-Krawczyk Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
The use of weighted multiple linear regression to estimate QTL × QTL × QTL interaction effects of winter wheat (Triticum aestivum L.) doubled-haploid lines
J Appl Genet. 2023 Oct 25. doi: 10.1007/s13353-023-00795-3. Online ahead of print.ABSTRACTKnowledge of the magnitude of gene effects and their interactions, their nature, and contribution to determining quantitative traits is very important in conducting an effective breeding program. In traditional breeding, information on the parameter related to additive gene effect and additive-additive interaction (epistasis) and higher-order additive interactions would be useful. Although commonly overlooked in studies, higher-order interactions have a significant impact on phenotypic traits. Failure to account for the effect of trip...
Source: J Appl Genet - October 25, 2023 Category: Genetics & Stem Cells Authors: Adrian Cyplik Dominika Piaskowska Pawe ł Czembor Jan Bocianowski Source Type: research
