Convergent evolution of allele-specific gene expression that leads to non-small cell lung cancer in different human populations
We present this novel angle that differential expression analysis should be performed separately for different alleles. Our ideas should greatly benefit the cancer community.PMID:38036772 | DOI:10.1007/s13353-023-00813-4 (Source: J Appl Genet)
Source: J Appl Genet - November 30, 2023 Category: Genetics & Stem Cells Authors: Qiuyu Hou Lifeng Shang Xu Chen Qiang Luo Liang Wei Chence Zhang Source Type: research
Convergent evolution of allele-specific gene expression that leads to non-small cell lung cancer in different human populations
We present this novel angle that differential expression analysis should be performed separately for different alleles. Our ideas should greatly benefit the cancer community.PMID:38036772 | DOI:10.1007/s13353-023-00813-4 (Source: J Appl Genet)
Source: J Appl Genet - November 30, 2023 Category: Genetics & Stem Cells Authors: Qiuyu Hou Lifeng Shang Xu Chen Qiang Luo Liang Wei Chence Zhang Source Type: research
Enumeration of olive derived lignan, pinoresinol for activity against recent Omicron variant spike protein for structure-based drug design, DFT, molecular dynamics simulations, and MMGBSA studies
This study focuses on virtual screening, docking, and molecular dynamic analysis; we aimed to identify therapeutic candidates for the SARS-CoV-2 variant Omicron based on their ability to inhibit non-structural proteins. We investigate the prediction of the properties of a substantial database of drug molecules obtained from the OliveNet™ database. Compounds that did not exhibit adequate gastrointestinal absorption and failed the Lipinski test are not considered for further research. The filtered compounds were coupled with our primary target, SARS-CoV-2 Omicron spike protein. We focused on SARS-CoV-2 Omicron spike protei...
Source: J Appl Genet - November 29, 2023 Category: Genetics & Stem Cells Authors: Samia S Alkhalil Shoruq E Alosaimi Manal E Alosaimi Zuhair M Mohammedsaleh Waleed Al Abdulmonem Abdullah Saleh Alkhamiss Ruqaih S Alghsham Abdullah S M Aljohani Abdullah F Shater Fayez M Saleh Hailah M Almohaimeed Mona H Soliman Source Type: research
In vitro evaluation of biofilm phenotypic and genotypic characteristics among clinical isolates of Pseudomonas aeruginosa in Hamadan, West of Iran
This study was performed to determine the antibiotic resistance pattern, biofilm formation, and frequency of biofilm-related genes in P. aeruginosa strains. In total, 123 P. aeruginosa isolates were collected from different clinical sources. Antimicrobial susceptibility testing (AST) was performed to detect multidrug-resistant P. aeruginosa (MDRPA) isolates. To evaluate the biofilm-forming isolates, the microtiter plate (MTP) method was carried out. Also, the prevalence of biofilm genotype patterns, including pslA, pslD, pelA, pelF, and algD genes, was detected by polymerases chain reaction (PCR). According to our findings...
Source: J Appl Genet - November 28, 2023 Category: Genetics & Stem Cells Authors: Jaber Hemmati Mohsen Nazari Amjad Ahmadi Maral Bayati Mahsa Jalili Mohammad Taheri Younes Mohammadi Babak Asghari Source Type: research
In vitro evaluation of biofilm phenotypic and genotypic characteristics among clinical isolates of Pseudomonas aeruginosa in Hamadan, West of Iran
This study was performed to determine the antibiotic resistance pattern, biofilm formation, and frequency of biofilm-related genes in P. aeruginosa strains. In total, 123 P. aeruginosa isolates were collected from different clinical sources. Antimicrobial susceptibility testing (AST) was performed to detect multidrug-resistant P. aeruginosa (MDRPA) isolates. To evaluate the biofilm-forming isolates, the microtiter plate (MTP) method was carried out. Also, the prevalence of biofilm genotype patterns, including pslA, pslD, pelA, pelF, and algD genes, was detected by polymerases chain reaction (PCR). According to our findings...
Source: J Appl Genet - November 28, 2023 Category: Genetics & Stem Cells Authors: Jaber Hemmati Mohsen Nazari Amjad Ahmadi Maral Bayati Mahsa Jalili Mohammad Taheri Younes Mohammadi Babak Asghari Source Type: research
High expression of ACKR1 predicts a good prognosis and suppresses sarcoma cell progression via regulating the tumor immune microenvironment
In conclusion, low expression of ACKR1 presented as an independent prognostic biomarker in sarcoma. Overexpression of ACKR1 can significantly suppress cell progression ability in sarcoma by regulating the immune microenvironment.PMID:38006471 | DOI:10.1007/s13353-023-00805-4 (Source: J Appl Genet)
Source: J Appl Genet - November 25, 2023 Category: Genetics & Stem Cells Authors: Jinluan Lin Fude Liang Lifeng Zheng Jinyuan Zeng Jianhua Lin Source Type: research
High expression of ACKR1 predicts a good prognosis and suppresses sarcoma cell progression via regulating the tumor immune microenvironment
In conclusion, low expression of ACKR1 presented as an independent prognostic biomarker in sarcoma. Overexpression of ACKR1 can significantly suppress cell progression ability in sarcoma by regulating the immune microenvironment.PMID:38006471 | DOI:10.1007/s13353-023-00805-4 (Source: J Appl Genet)
Source: J Appl Genet - November 25, 2023 Category: Genetics & Stem Cells Authors: Jinluan Lin Fude Liang Lifeng Zheng Jinyuan Zeng Jianhua Lin Source Type: research
High expression of ACKR1 predicts a good prognosis and suppresses sarcoma cell progression via regulating the tumor immune microenvironment
In conclusion, low expression of ACKR1 presented as an independent prognostic biomarker in sarcoma. Overexpression of ACKR1 can significantly suppress cell progression ability in sarcoma by regulating the immune microenvironment.PMID:38006471 | DOI:10.1007/s13353-023-00805-4 (Source: J Appl Genet)
Source: J Appl Genet - November 25, 2023 Category: Genetics & Stem Cells Authors: Jinluan Lin Fude Liang Lifeng Zheng Jinyuan Zeng Jianhua Lin Source Type: research
Molecular complexity of diffuse large B-cell lymphoma: a molecular perspective and therapeutic implications
In conclusion, this review underscores the remarkable strides in understanding DLBCL's molecular underpinnings, spanning genetics, cell signaling, the tumor microenvironment, and epigenetics. These advances pave the way for more effective, personalized treatments, renewing hope for DLBCL patients.PMID:38001281 | DOI:10.1007/s13353-023-00804-5 (Source: J Appl Genet)
Source: J Appl Genet - November 24, 2023 Category: Genetics & Stem Cells Authors: Hibah Ali Almasmoum Source Type: research
Molecular complexity of diffuse large B-cell lymphoma: a molecular perspective and therapeutic implications
In conclusion, this review underscores the remarkable strides in understanding DLBCL's molecular underpinnings, spanning genetics, cell signaling, the tumor microenvironment, and epigenetics. These advances pave the way for more effective, personalized treatments, renewing hope for DLBCL patients.PMID:38001281 | DOI:10.1007/s13353-023-00804-5 (Source: J Appl Genet)
Source: J Appl Genet - November 24, 2023 Category: Genetics & Stem Cells Authors: Hibah Ali Almasmoum Source Type: research
Identification of co-expressed genes and immune infiltration features related to the progression of atherosclerosis
In this study, gene co-expression network analysis (WGCNA) was done to identify gene co-expression modules associated with atherosclerosis progression. Before conducting WGCNA, preprocessing and soft power selection were performed on the GSE28829, GSE100927, GSE43292, GSE10334, and GSE16134 datasets ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi ). Co-expression modules were identified using dynamic tree cuts, and their correlations and trait associations were visualized. Enrichment analysis was performed on the blue and magenta modules to identify biological processes (BP) and pathways related to atherosclerosis. The CI...
Source: J Appl Genet - November 23, 2023 Category: Genetics & Stem Cells Authors: Junqing Gu Wenwei Yang Shun Lin Danqing Ying Source Type: research
NADPH oxidase 4-mediating oxidative stress contributes to endometriosis
This study aims to investigate the effects of NOX4 in endometriosis and its molecular mechanisms. Clinical specimens were collected, and human endometrial stromal cells (HESCs) were isolated. The knockdown of NOX4 cell lines was established on the HESCs and induced by peritoneal fluid. The levels of NOX4 were determined by using immunohistochemistry (IHC) staining, western blotting, and qPCR, respectively. The levels of oxidative stress markers were determined by using western blotting and ELISAs, respectively. The correlation of NOX4 and oxidative stress markers was analyzed by the Pearson correlation coefficient. The lev...
Source: J Appl Genet - November 22, 2023 Category: Genetics & Stem Cells Authors: Xiaojie Wang Xiaona Jiang Xin Lv Xinshu Wang Aimin Lin Yangyang Li Source Type: research
Modulation of lncRNA NEAT1 overturns the macrophages based immune response in M. tuberculosis infected patients via miR-373 regulation
J Appl Genet. 2023 Nov 23. doi: 10.1007/s13353-023-00808-1. Online ahead of print.ABSTRACTThere is a lack of studies which explore and clarify the interactions that occur between host macrophage and Mycobacterium tuberculosis with regard to microRNA such as LNCNEAT1 and miR-373. The current study determines the mechanisms involved in the control of M. tuberculosis infection by macrophage using LNCNEAT1 and miR-373. The researchers collected different samples from healthy individuals, pulmonary TB patients, and samples like hMDMs cells and H37Rv infected MTB to determine the concentrations of inflammatory factors. The impac...
Source: J Appl Genet - November 22, 2023 Category: Genetics & Stem Cells Authors: Fahad M Aldakheel Rabbani Syed Musthaq Ahmed Tao Xu Source Type: research
NADPH oxidase 4-mediating oxidative stress contributes to endometriosis
This study aims to investigate the effects of NOX4 in endometriosis and its molecular mechanisms. Clinical specimens were collected, and human endometrial stromal cells (HESCs) were isolated. The knockdown of NOX4 cell lines was established on the HESCs and induced by peritoneal fluid. The levels of NOX4 were determined by using immunohistochemistry (IHC) staining, western blotting, and qPCR, respectively. The levels of oxidative stress markers were determined by using western blotting and ELISAs, respectively. The correlation of NOX4 and oxidative stress markers was analyzed by the Pearson correlation coefficient. The lev...
Source: J Appl Genet - November 22, 2023 Category: Genetics & Stem Cells Authors: Xiaojie Wang Xiaona Jiang Xin Lv Xinshu Wang Aimin Lin Yangyang Li Source Type: research
Modulation of lncRNA NEAT1 overturns the macrophages based immune response in M. tuberculosis infected patients via miR-373 regulation
J Appl Genet. 2023 Nov 23. doi: 10.1007/s13353-023-00808-1. Online ahead of print.ABSTRACTThere is a lack of studies which explore and clarify the interactions that occur between host macrophage and Mycobacterium tuberculosis with regard to microRNA such as LNCNEAT1 and miR-373. The current study determines the mechanisms involved in the control of M. tuberculosis infection by macrophage using LNCNEAT1 and miR-373. The researchers collected different samples from healthy individuals, pulmonary TB patients, and samples like hMDMs cells and H37Rv infected MTB to determine the concentrations of inflammatory factors. The impac...
Source: J Appl Genet - November 22, 2023 Category: Genetics & Stem Cells Authors: Fahad M Aldakheel Rabbani Syed Musthaq Ahmed Tao Xu Source Type: research
