Evaluation of seminal fluid leukocyte subpopulations in patients with varicocele.
Authors: Mongioì LM, Alamo A, Calogero AE, Compagnone M, Giacone F, Cannarella R, La Vignera S, Condorelli RA Abstract Varicocele is a common cause of sperm damage. Some studies showed higher concentration of seminal leukocytes in patients with varicocele. The aim of the study was to evaluate seminal leukocyte subpopulations in patients with varicocele. We enrolled 20 patients with varicocele and 20 age-matched healthy men. Sperm analysis was conducted according to the World Health Organization (WHO) 2010 criteria. We evaluated seminal leukocyte subpopulations and bio-functional sperm parameters by flow cyto...
Source: International Journal of Immunopathology and Pharmacology - July 10, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Typhae pollen polysaccharides protect hypoxia-induced PC12 cell injury via regulation of miR-34a/SIRT1.
In conclusion, TPP plays important roles in regulating cell viability and apoptosis in hypoxia-treated PC12 cells via modulating miR-34a/SIRT1, as well as activating PI3K/AKT and Ras/Raf/MEK/ERK signal pathways. PMID: 32635836 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - July 10, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

A previously treated severe haemophilia A patient developed high-titre inhibitor after vaccinations.
We reported a previously treated SHA patient postponed the first vaccination to 15-month age received diphtheria-pertussis-tetanus intramuscularly. At 18-month age, the patient received Hepatitis A intramuscularly and Varicella Zoster Virus subcutaneously with 2 weeks interval and FVIII infusion was given
Source: International Journal of Immunopathology and Pharmacology - July 7, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Upregulation of the miRNA-155, miRNA-210, and miRNA-20b in psoriasis patients and their relation to IL-17.
This study aimed at assessing the expression of miRNA-155, miRNA-210, and miRNA-20b in skin and sera of psoriasis patients in relation to IL-17 levels. For 20 psoriasis patients and 20 matching controls, the expression of miRNA-155, miRNA-210, and miRNA-20b was assessed using real-time polymerase chain reaction (RT-PCR), whereas IL-17/IL-17A levels were measured using quantitative enzyme-linked immunosorbent assay (ELISA) technique. MiRNA-155 expression was significantly higher in lesional skin compared to controls (P = 0.001). MiRNA-210 expression was significantly higher in both, lesional skin (P =&t...
Source: International Journal of Immunopathology and Pharmacology - July 2, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

External validation of the sepsis severity score.
CONCLUSION: The SSS demonstrated excellent discrimination. The calibration evaluation gave conflicting results; the H-L test result indicated a good calibration, while the visual analysis of the calibration curve suggested the opposite. The SSS requires further evaluation before it can be safely recommended as an outcome prediction model. PMID: 32602801 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - July 2, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Phloretin alleviates dinitrochlorobenzene-induced dermatitis in BALB/c mice.
In this study, we utilized a natural, plant-derived phenolic compound, phloretin, to treat allergic contact dermatitis (ACD) on the dorsal skin of mice. In addition, the effectiveness of phloretin was evaluated using a mouse model of ACD triggered by 2,4-dinitrochlorobenzene (DNCB). In our experimental setting, phloretin was orally administered to BALB/c mice for 21 consecutive days, and then, the lesions were examined histologically. Our data revealed that phloretin reduced the process of epidermal thickening and decreased the infiltration of mast cells into the lesion regions, subsequently reducing the levels of histamin...
Source: International Journal of Immunopathology and Pharmacology - June 24, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Renal cell carcinoma associated with idiopathic thrombocytopenic purpura.
We reported a 56-year-old man who presented with petechiae and ecchymoses. Laboratory studies showed the platelet count of 2 × 109/L and an abdominal computed tomography (CT) scan revealed tumors in the right renal. There were purpura on the legs and cough without abdominal pain and melena at this time. Idiopathic thrombocytopenic purpura was diagnosed according to the clinical symptoms and laboratory test. The patient received radical nephrectomy for renal carcinoma, and his idiopathic thrombocytopenic purpura was cured after the surgery. Pathological biopsy confirmed it was renal clear cell carcinoma....
Source: International Journal of Immunopathology and Pharmacology - June 23, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Impact of nickel oral hyposensitization on quality of life in systemic nickel allergy syndrome.
Authors: Rizzi A, Di Rienzo A, Buonomo A, Aruanno A, Carusi V, Ricci AG, Centrone M, Mezzacappa S, Romeo L, Schiavino D, Inchingolo R, Gasbarrini A, Nucera E Abstract Nickel (Ni) oral hyposensitization treatment (NiOHT) is an effective management approach for Ni allergy. No health-related quality of life (HRQoL) data exist for the pre- and post-treatment with NiOHT in systemic nickel allergy syndrome (SNAS). The aims of this study were (a) to explore HRQoL in SNAS patients, (b) to assess changes of HRQoL after 1 year of NiOHT; (c) to evaluate psychological status of patients. SNAS patients completed the Shor...
Source: International Journal of Immunopathology and Pharmacology - June 23, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Pediatric pharmaceutical care with anti-infective medication in a patient with acute hematogenous osteomyelitis caused by methicillin-resistant Staphylococcus aureus.
In this study, we first report a case of a child with MRSA-caused osteomyelitis who was successfully cured by VCM at a concentration of 4.86 μg/mL. VCM's clinical daily dose of more than 4 g was of concern in light of recent evidence suggesting the increased risks of nephrotoxicity and red man syndrome when Cmin ⩾15 μg/mL and doses ⩾10 mg/kg in children. As far as we know, this is the first report on the lower dose of VCM in children with MRSA osteomyelitis. PMID: 32567423 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - June 23, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Characterization of lymphocyte subsets in ascitic fluid and peripheral blood of decompensated cirrhotic patients with chronic hepatitis C and alcoholic liver disease: A pivotal study.
Authors: Romanelli RG, Vitiello G, Gitto S, Giudizi MG, Biagiotti R, Carraresi A, Vizzutti F, Laffi G, Almerigogna F Abstract Hepatitis C virus and alcoholic liver disease are major causes of chronic liver diseases worldwide. Little is known about differences between chronic hepatitis C and alcoholic liver disease in terms of lymphocytes' sub-population. Aim of the present study was to compare the sub-populations of lymphocytes in both ascitic compartment and peripheral blood in patients with decompensated liver cirrhosis due to chronic hepatitis C and alcoholic liver disease. Patients with decompensated liver cirr...
Source: International Journal of Immunopathology and Pharmacology - June 13, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Gender differences in audio-vestibular disorders.
Authors: Corazzi V, Ciorba A, Skarżyński PH, Skarżyńska MB, Bianchini C, Stomeo F, Bellini T, Pelucchi S, Hatzopoulos S Abstract In the last years, the attention to the role of gender in physiopathology and pharmacology of diseases in several medical disciplines is rising; however, the data on the relationship between gender and audio-vestibular disorders are still inconclusive and sometimes confusing. With this letter to the editor, we would like to review the role of gender in audio-vestibular disorders. Literature data show that anatomic variances of the inner ear do exist in men and women and that the diffe...
Source: International Journal of Immunopathology and Pharmacology - June 13, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Non-invasive papillary urothelial carcinoma, low-grade of the renal pelvis mimicking a xanthogranulomatous pyelonephritis in a male patient: A case report and review of literature.
We report on a 31-year-old male patient with non-invasive papillary urothelial carcinoma, low grade of the renal pelvis disguised as xanthogranulomatous pyelonephritis. The only symptom of the patient was lower back pain. The initial renal-enhanced computed tomography, magnetic resonance imaging and contrast-enhanced ultrasonography showed that the right kidney had a benign lesion and this inflammatory lesion might be xanthogranulomatous pyelonephritis. A percutaneous renal biopsy was performed and histopathologic examination revealed a xanthogranulomatous pyelonephritis. Initially, we diagnosed it as xanthogranulomatous p...
Source: International Journal of Immunopathology and Pharmacology - June 5, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Study on myelin injury of AD mice treated with Shenzhiling oral liquid in the PI3K/Akt-mTOR pathway.
Authors: Wang Y, Chen F, Wang P, Mana L, Sheng N, Huang S Abstract Shenzhiling oral liquid (SZL) is a Traditional Chinese Medicine (TCM) compound to be approved by the China Food and Drug Administration (CFDA) (Z20120010) for the treatment of mild-to-moderate Alzheimer's disease (AD). However, its mechanism in early AD is not clear. We studied its mechanism in protecting myelin. Three-month-old APPswe/PS1dE9double transgenic mice were used as AD model and wild-type C57BL/6 mice were used as control. After 3-month intervention, the Morris water maze was used to detect behavioural changes. Myelin mTOR pathway (PI3K, ...
Source: International Journal of Immunopathology and Pharmacology - May 30, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Amelioration of paraquat-induced pulmonary fibrosis in mice by regulating miR-140-5p expression with the fibrogenic inhibitor Xuebijing.
Authors: Dong MN, Xiao Y, Li YF, Wang DM, Qu YP, Fang TW, Li H, Liu MW Abstract Intravenous Xuebijing (XBJ) therapy suppresses paraquat (PQ)-induced pulmonary fibrosis. However, the mechanism underlying this suppression remains unknown. This work aimed to analyze the miR-140-5p-induced effects of XBJ injection on PQ-induced pulmonary fibrosis in mice. The mice were arbitrarily assigned to four groups. The model group was administered with PQ only. The PQ treatment group was administered with PQ and XBJ. The control group was administered with saline only. The control treatment group was administered with XBJ only. ...
Source: International Journal of Immunopathology and Pharmacology - May 30, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Shrimp sensitization in house dust mite allergic patients.
Authors: Ukleja-Sokołowska N, Gawrońska-Ukleja E, Lis K, Żbikowska-Gotz M, Adamczak R, Sokołowski Ł, Bartuzi Z Abstract Shrimp tropomyosin has a similar structure to house dust mite (HDM) tropomyosin. In this research, 232 adult patients with symptoms of persistent allergic rhinitis were randomly selected. In the group, 59% were sensitized to Dermatophagoides pteronyssinus and 57.8% to Dermatophagoides farinae. In total, 128 (55.2%) patients were sensitized to both HDM species and 143 (61.6%) to at least one. Slightly over a quarter (25.4%) of patients were sensitized to shrimp. Of the 35 shrimp-sensitized pat...
Source: International Journal of Immunopathology and Pharmacology - March 8, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile.
Authors: Bautista-Herrera LA, De la Cruz-Mosso U, Román-Fernández IV, Parra-Rojas I, Soñanez-Organis JG, Hernández-Bello J, Morales-Zambrano RA, Villanueva-Quintero GD, Muñoz-Valle JF Abstract The goals of our study were to determine the possible association of interleukin (IL)-31 with Th17 cytokine profile in serum and to quantify retinoic acid-related orphan receptor C (RORC) mRNA expression in psoriatic arthritis (PsA) patients. This cross-sectional study was conducted in 50 patients with PsA and 30 control subjects (CS) matched by age and gender. The cytokine serum levels were...
Source: International Journal of Immunopathology and Pharmacology - March 8, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

miR-142-5p regulates the progression of diabetic retinopathy by targeting IGF1.
Authors: Liu X, Li J, Li X Abstract As one of leading causes of blindness, diabetic retinopathy (DR) is a progressive microvascular complication of diabetes mellitus (DM). Despite significant efforts have been devoted to investigate DR over the years, the molecular mechanisms still remained unclear. Emerging evidences demonstrated that microRNAs (miRNAs) were tightly associated with pathophysiological development of DR. Hence, this study was aimed to illustrate the role and molecular mechanisms of miR-412-5p in progression of DR. Streptozotocin (STZ) treatment in rats and human retinal endothelial cell (HREC) model...
Source: International Journal of Immunopathology and Pharmacology - March 4, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Urogenital infections in patients with diabetes mellitus: Beyond the conventional aspects.
In conclusion, urogenital infections may more likely lead to complication in diabetic than non-diabetic patients, affect fertility and glucose control. Therefore, they need proper management. PMID: 32031031 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - February 8, 2020 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Association between oral cleft and transcobalamin 2 polymorphism in a sample study from Nassiriya, Iraq.
Authors: Carinci F, Palmieri A, Scapoli L, Cura F, Abenavoli F, Giannì AB, Russillo A, Docimo R, Martinelli M Abstract Orofacial clefts are common congenital defects whose prevalence differs between geographical regions and ethnic groups. The inheritance is complex, involving the contribution of both genetic and environmental factors. The involvement of genes belonging to the folate pathway is still matter of debate, with strong evidences of association and conflicting results. After demonstrating the contribution, for a sample from the Italian population, of common mutations mapping on three genes of the fo...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

A hydrosilver gel for plaque control in adults affected by chronic periodontitis: Effects on the 'red complex' bacterial load. A prospective longitudinal pilot study using polymerase chain reaction analysis.
Authors: Lauritano D, Nota A, Martinelli M, Severino M, Romano M, Rossi D, Caruso S Abstract In subjects affected by chronic periodontitis, the chemical control of plaque is a strategy aiming primarily at controlling infection and bacterial loading. The aim is to evaluate the bacterial loading of the so-called 'red complex' associated with a short-term use of a hydrosilver gel (HSG) by using an in vivo model in adult subjects affected by chronic periodontitis. This prospective short-term clinical trial involved 10 adult volunteers using a 15-day in vivo model. After receiving professional prophylaxis at baseline (t...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Endosseous distal extension (EDE) blade implant technique useful to provide stable pillars in the ipotrophic lower posterior sector: 22  years statistical survey.
Endosseous distal extension (EDE) blade implant technique useful to provide stable pillars in the ipotrophic lower posterior sector: 22 years statistical survey. Int J Immunopathol Pharmacol. 2019 Jan-Dec;33:2058738419838092 Authors: Dal Carlo L, Pasqualini M, Shulman M, Rossi F, Comola G, Manenti P, Candotto V, Lauritano D, Zampetti P Abstract The aim of this issue is to describe endosseous distal extension (EDE) surgical technique. This implant technique was conceived and applied since 1993 by Dr Luca Dal Carlo, as an evolution of the classical ramus blade implant technique. With this techniqu...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

The use of a new chemical device based on silver and cationic surfactants as a new approach for daily oral hygiene: A preliminary study on a group of periodontal patients.
Authors: Dorina L, Annalisa P, Ornella D, Alessandro B, Liliana O, Marco G, Di Girolamo M, Valentina C Abstract The aim of this study was to evaluate the abatement power of oral microbial loading of a new gel formulation based on the complex silver-2-mercaptobenzoate, chlorhexidine digluconate and didecyldimethylammonium chloride (named ADC) through polymerase chain reaction (PCR). The study sample consists of a group of 20 patients with chronic periodontal disease. Patients were over 25 years of age and did not undergo surgical or non-surgical periodontal treatment in the previous 6 months. The study sample was al...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Berberine and Tinospora cordifolia exert a potential anticancer effect on colon cancer cells by acting on specific pathways.
Authors: Palmieri A, Scapoli L, Iapichino A, Mercolini L, Mandrone M, Poli F, Giannì AB, Baserga C, Martinelli M Abstract Berberine (BBR) is a natural active principle with potential antitumor activity. The compound targets multiple cell signaling pathways, including proliferation, differentiation, and epithelial-mesenchymal transition. The aim of this study was to elucidate the mechanisms behind the anticancer activity of BBR by comparing the effects of purified BBR with those of the extract of Tinospora cordifolia, a medicinal plant that produces this metabolite. The expression levels of a panel of 44 sele...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Copy number variation analysis of twin pairs discordant for cleft lip with or without cleft palate.
Authors: Scapoli L, Carinci F, Palmieri A, Cura F, Baj A, Beltramini G, Docimo R, Martinelli M Abstract Non-syndromic cleft lip with or without cleft palate (nsCL/P) is a frequent orofacial malformation. The comparison of concordance rate observed in monozygotic and dizygotic twins supports high level of heritability and a strong genetic component. However, phenotype concordance for orofacial cleft in monozygotic twins is about 50%. The aim of the present investigation was to detect postzygotic events that may account for discordance in monozygotic twins. High-density SNP microarrays hybridization was used to genot...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Phenytoin and gingival mucosa: A molecular investigation.
Authors: Candotto V, Pezzetti F, Baj A, Beltramini G, Lauritano D, Di Girolamo M, Cura F Abstract Several distinct classes of drugs, such as anticonvulsants, immunosuppressants, and calcium channel blockers, caused gingival overgrowth. One of the main drugs associated with the gingival overgrowth is the anti-epileptic such as phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. In our study, we evaluate the effect of phenytoin, a drug whose active substance is phenytoin, on gingival fibroblasts of healthy volunteers. Gene expression of 29 genes was investigated in gingival fibrobla...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Non-syndromic cleft palate: Association analysis on three gene polymorphisms of the folate pathway in Asian and Italian populations.
Authors: Carinci F, Palmieri A, Scapoli L, Cura F, Borelli F, Morselli PG, Nouri N, Abdali H, Gianni AB, Russillo A, Docimo R, Martinelli M Abstract Periconceptional folic acid supplementation can reduce the risk of inborn malformations, including orofacial clefts. Polymorphisms of MTHFR, TCN2, and CBS folate-related genes seem to modulate the risk of cleft lip with or without cleft palate (CL/P) in some populations. CL/P and cleft palate only (CPO) are different malformations that share several features and possibly etiological causes. In the present investigation, we conducted a family-based, candidate gene assoc...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Gabapentin affects the expression of inflammatory mediators on healthy gingival cells.
Authors: Candotto V, Scapoli L, Gaudio RM, Gianni AB, Bolzoni A, Racco P, Lauritano D, Cura F Abstract Gabapentin is one of the most used drugs to treat postoperative pain with antihyperalgesic properties and has a unique mechanism of action, which differentiates it from other commonly used drugs. Various studies have shown that the perioperative use of gabapentin reduces postoperative pain. In our study, fragments of gingival tissue of healthy volunteers were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblasts cell culture treated with gabapentin, compared with untreate...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Drug-induced gingival hyperplasia: An in vitro study using amlodipine and human gingival fibroblasts.
Authors: Lauritano D, Martinelli M, Baj A, Beltramini G, Candotto V, Ruggiero F, Palmieri A Abstract Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast's function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during oper...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Evaluation of IL6, IL10 and VDR alleles distribution in an Italian large sample of subjects affected by chronic periodontal disease.
Authors: Luca S, Francesco C, Damiano M, Alessandro N, Silvia C, Diego R, Michele R, Marco S Abstract In recent decades, the role played by the immune response to bacteria in the pathogenesis of chronic periodontal disease (PD) has long been studied. Although from the clinical point of view, adequate oral hygiene is essential to ensure a satisfactory response of the host to infections, modulation of the reaction of immune system could be influenced by genetic factors. The aim of the present study was to investigate the distribution of alleles of polymorphisms relevant for chronic periodontitis in a sample of adult ...
Source: International Journal of Immunopathology and Pharmacology - November 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Antitumor activity of enzymatically hydrolyzed Ganoderma lucidum polysaccharide on U14 cervical carcinoma-bearing mice.
Authors: Kong M, Yao Y, Zhang H Abstract Polysaccharides from Ganoderma lucidum have been demonstrated to possess diverse biological activities. Despite lots of studies on the biological activities of Ganoderma lucidum polysaccharide (GLP), little is known regarding the medicinal potential of low-molecular weight enzymatically hydrolyzed Ganoderma lucidum polysaccharide (EGLP). EGLP was prepared by enzymatic degradation and its potential effects in U14 cervical tumor-bearing mice were evaluated. Both GLP and EGLP delayed tumor growth of the tumor xenograft. The EGLP was superior to native polysaccharide. Moreover, ...
Source: International Journal of Immunopathology and Pharmacology - August 31, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

MicroRNA-103 regulates the progression in endometrial carcinoma through ZO-1.
In conclusion, miR-103 could regulate EC cell proliferation through directly targeting ZO-1. Our results provide a potential development of microRNA-based targeted approaches for the treatment of EC. PMID: 31456452 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - August 30, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Green tea polyphenols protect PC12 cells against H2O2-induced damages by upregulating lncRNA MALAT1.
In this study, we aimed to investigate the protective effects of GTP against H2O2-caused injuries in PC12 cells as well as the molecular underpinnings associated with long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). PC12 cells were preincubated with GTP prior to H2O2 stimulation. Furthermore, MALAT1-deficient PC12 cells were constructed by transfection and identified by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Next, viability and apoptosis were detected by cell counting kit-8 and flow cytometry, respectively. Meanwhile, Western blot assay was carried out to monit...
Source: International Journal of Immunopathology and Pharmacology - August 30, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Efficacy and safety of eltrombopag in the treatment of severe chronic immune thrombocytopenia in children of China: A single-center observational study.
Authors: Cheng X, Yan K, Ma J, Chen Z, Zhao L, Wang X, Wu R Abstract The treatment of severe chronic immune thrombocytopenia (SCITP) in pediatric patients is challenging. We evaluated the clinical efficacy and safety of eltrombopag in children with SCITP in China. This observational study was carried out at the Hematology Oncology Center, Beijing Children's Hospital between April 2017 and July 2018. Patients with SCITP who had at least 12 weeks of eltrombopag treatment and follow-up data were included. Baseline data, such as age, drug dosage, pre-study platelet count, concomitant medications, and bleeding se...
Source: International Journal of Immunopathology and Pharmacology - August 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

The combination of Astragalus membranaceus extract and ligustrazine to improve the inflammation in rats with thrombolytic cerebral ischemia.
Authors: Pan R, Zhou M, Zhong Y, Xie J, Ling S, Tang X, Huang Y, Chen H Abstract The purpose of the study was to evaluate the effect of Astragalus membranaceus extract and ligustrazine combination on ameliorating inflammation in cerebral ischemic rats that have undergone thrombolysis. Astragalus membranaceus and ligustrazine per se, or a combination of A. membranaceus and ligustrazine was administered by intraperitoneal injection immediately after surgery and sham surgery. After the induction of thrombolysis, the neurological function was measured and cerebral lesion volume was determined. The regulatory T cells in...
Source: International Journal of Immunopathology and Pharmacology - August 16, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Inflammatory bowel disease: Looking beyond the tract.
Authors: Chams S, Badran R, Sayegh SE, Chams N, Shams A, Hajj Hussein I Abstract Inflammatory bowel disease is a chronic inflammatory condition that encompasses Crohn's disease and ulcerative colitis. Inflammatory bowel disease is not exclusive to the gastrointestinal system, as it has been identified to be associated with extraintestinal manifestations that encompass every other organ system in the human body. This review article will comprehensively review the current knowledge on extraintestinal manifestations of inflammatory bowel disease. In addition, it will discuss the recommendations for screening and surve...
Source: International Journal of Immunopathology and Pharmacology - August 7, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Protective effects of ginsenoside Rb1 on H2O2-induced oxidative injury in human endothelial cell line (EA.hy926) via miR-210.
Authors: Jia F, Mou L, Ge H Abstract Ginsenoside Rb1 (Rb1) possesses a cardioprotective effect via mediating microRNAs (miRs), while it is unexplored whether miR-210 is regulated by Rb1 in response to oxidative stress. Human endothelial EA.hy926 cells were stimulated with H2O2 before Rb1 treatment. After transfection, cell viability, apoptosis, migration, and invasion assays were conducted. Western blot was applied to quantify protein. BCL2/adenovirus E1B 19-kDa interacting protein 3 (BNIP3) and miR-210 were analyzed with quantitative reverse transcription polymerase chain reaction. Dual luciferase activity assay w...
Source: International Journal of Immunopathology and Pharmacology - August 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Berberine reduces neuroglia activation and inflammation in streptozotocin-induced diabetic mice.
Authors: Liu M, Gao L, Zhang N Abstract We aimed to analyze the action of berberine on the neuropathic pain and neuroglia activation in experimental diabetes mellitus (DM) model. Diabetes in mice was induced by intraperitoneal injection of streptozotocin (STZ) followed by the administration of berberine. Mechanical allodynia and thermal hyperalgesia and activations of microglia and astrocytes were evaluated. The levels of pro-inflammatory cytokines and protein expressions of inflammatory proteins were assessed by enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. Our results revealed the anti...
Source: International Journal of Immunopathology and Pharmacology - July 25, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effects of GIT-27NO, a NO-donating compound, on hepatic ischemia/reperfusion injury.
In this study, we have investigated the effects of this compound in vitro, in a model of oxidative stress induced in HepG2 cells by hydrogen peroxide (H2O2), and in vivo, in a rat model of IRI of the liver. GIT-27NO significantly counteracted the toxic effects induced by the H2O2 on the HepG2 cells and in vivo, GIT-27NO reduced the transaminase levels and the histological liver injury by reducing necrotic areas with preservation of viable tissue. These effects were almost similar to that of the positive control drug dimethyl fumarate. These data suggest that the beneficial effect of GIT-27NO in the hepatic IRI can be secon...
Source: International Journal of Immunopathology and Pharmacology - July 14, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Regulatory T-cells and their impacts on cytokine profile of end-stage renal disease patients suffering from systemic lupus erythematosus.
This study aimed to determine Treg frequency and its effects on cytokine profile of ESRD patients with and without systemic lupus erythematosus (SLE). Moreover, this study also determines how Treg number is affected by blood transfusion and gender. Peripheral blood mononuclear cells were isolated from 26 ESRD and 10 healthy subjects and stained with anti-CD4, anti-CD25, and anti-FoxP3 antibodies. Treg frequencies in ESRD patients with and without blood transfusion were determined by flow cytometry. Antibodies against human leukocyte antigens (HLAs) were investigated by panel-reactive antibodies screening. Tumor growth fact...
Source: International Journal of Immunopathology and Pharmacology - July 11, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Primary prostate lymphoma: A case report and literature review.
This article reports a case of PPL by retrospective analysis of the clinical data and review of the literature, in an attempt to explore the diagnosis, treatment, and prognosis of this rare disease. The present case involves a male patient who came to our hospital for medical consultation of dysuria. Serum prostate-specific antigen (PSA) was not remarkable. Pelvic computed tomography (CT) scan suggested obvious enlargement of the prostate and a tumor in the prostate, but the tumor was not significantly enhanced on contrast-enhanced CT scan. Ultrasound suggested obvious enlargement of the prostate with multiple local low-de...
Source: International Journal of Immunopathology and Pharmacology - July 11, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Curcumin inhibits the lymphangiogenesis of gastric cancer cells by inhibiton of HMGB1/VEGF-D signaling.
In this study, the cytotoxic effects of curcumin were investigated in gastric cancer AGS and SGC-7901 cell lines by MTT assay, and curcumin-induced morphological changes and cell apoptosis were assessed by using flow cytometry analysis and caspase-3 activity. The effects of curcumin on HMGB1 and VEGF-D expression were examined by reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis. As a result, we found that curcumin decreased cell viability and caused a dose-dependent cell apoptosis through the activation of caspase-3. The mRNA and protein expression levels of HMGB1 and VEGF-D were significa...
Source: International Journal of Immunopathology and Pharmacology - July 5, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Sulforaphane alleviates retinal ganglion cell death and inflammation by suppressing NLRP3 inflammasome activation in a rat model of retinal ischemia/reperfusion injury.
This study aims to study the potentials of sulforaphane (SFN) against retinal ischemia/reperfusion (I/R) injury. A rat retinal I/R injury method was established. Retinal thickness change and retinal ganglion cell (RGC) death were determined using hematoxylin and eosin (H&E) staining and Fluoro-Gold (FG) labeling. The inflammatory cytokines production and microglia activation were evaluated by using quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and enzyme-linked immunosorbent assay (ELISA). Knockdown NLRP3 was performed, and the according changes of retinal RGCs were assessed. SFN administrat...
Source: International Journal of Immunopathology and Pharmacology - July 5, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

The effect of perirenal puncture and drainage with urokinase treatment of perirenal hematoma complicated by infection: Two cases' report.
Authors: Li B, Li D, Liang T, Li X Abstract The aim of this study was to discuss the clinical significance of perirenal puncture and drainage with urokinase treatment of perirenal hematoma complicated by infection following surgery for upper urinary tract calculi. Two cases of perirenal hematoma complicated by infection following surgery for upper urinary tract calculi in 2017, and later received perirenal puncture and drainage of perirenal hematoma with urokinase treatment were selected. Puncture and drainage of perirenal hematoma with urokinase treatment were performed without the occurrence of severe complicatio...
Source: International Journal of Immunopathology and Pharmacology - June 28, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

LncRNA MALAT1 contributes to non-small cell lung cancer progression via modulating miR-200a-3p/programmed death-ligand 1 axis.
In conclusion, LncRNA MALAT1 facilitates the progression of NSCLC by modulating miR-200a-3p/PDL1 axis. PMID: 31240979 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - June 28, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effect of stress on learning and motivation-relevance to autism spectrum disorder.
Authors: Theoharides TC, Kavalioti M Abstract Learning and motivation are critical in the development of children, and to their acquisition of knowledge and skills. A case in point is autism spectrum disorder (ASD), a neurodevelopmental condition characterized by impaired social interactions and communication, as well as by stereotypic movements. Maternal stress has been strongly associated with increased risk of developing ASD. Children experience multiple stressors such as separation anxiety, fear of the unknown, physical and/or emotional trauma, bullying, as well as environmental exposures. Stress is well known ...
Source: International Journal of Immunopathology and Pharmacology - June 22, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Skullcapflavone I protects cardiomyocytes from hypoxia-caused injury through up-regulation of lincRNA-ROR.
This study explored the possible protective activity of Skullcapflavone I (SF I), a flavonoid isolated from the root of Scutellaria baicalensis Georgi, on hypoxia-stimulated cardiomyocytes cell injury in vitro. Viability and apoptosis of H9c2 cells and primary cardiomyocytes were tested using cell counting kit-8 (CCK-8) assay and Guava Nexin Reagent, respectively. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the long non-coding RNA regulator of reprogramming (lincRNA-ROR) expression. si-ROR was transfected to knockdown lincRNA-ROR. Western blotting was conducted to assess the p...
Source: International Journal of Immunopathology and Pharmacology - June 22, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Notoginsenoside R1 protects human keratinocytes HaCaT from LPS-induced inflammatory injury by downregulation of Myd88.
Authors: Zhang J, Zheng Q, Lu H, Jin F, Li Y, Bi F, Xu J Abstract Burn injury is a gigantic challenge in public health which brings multiple negative effects to patients both in physical and spiritual aspects. Inflammation plays vital roles in the progression of burn injury, and our study investigated whether notoginsenoside R1 (NGR1) alleviated lipopolysaccharide (LPS)-induced human keratinocyte HaCaT cell inflammatory injury. Inflammatory injury was induced by LPS in HaCaT cells. Stimulated cells were then treated by NGR1 in different concentrations. Cell viability and cell apoptosis were detected by Cell Countin...
Source: International Journal of Immunopathology and Pharmacology - June 18, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Searching for highly sensitive and specific biomarkers for sepsis: State-of-the-art in post-mortem diagnosis of sepsis through immunohistochemical analysis.
Authors: La Russa R, Maiese A, Viola RV, De Matteis A, Pinchi E, Frati P, Fineschi V Abstract The autoptical observations commonly ascribed to sepsis deal with unspecific general and local signs of inflammation or ischemia, such as myocardial inflammation, pulmonary edema and infiltration, cerebral swallowing, and tubular necrosis in the kidney. In the two last decades, some studies have been carried out to implement immunohistochemical markers for post-mortem diagnosis. All of these target molecules are specifically up-regulated or down-regulated during systemic inflammatory responses, especially for infective cau...
Source: International Journal of Immunopathology and Pharmacology - June 11, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Genetic variants on chromosome 9p21 confer risks of cerebral infarction in the Chinese population: a meta-analysis.
Authors: Chen JX, Liu J, Hu F, Bi Y, Li M, Zhao L Abstract Ischemic stroke (IS) is the leading cause of disability. Researchers have demonstrated that IS is more a multifactorial disorder than a single-factor disease. At present, no consistent conclusions have been reached on susceptibility loci for IS on chromosome 9p21. We conducted this meta-analysis to verify whether genetic loci on chromosome 9p21 reported domestically and abroad could be responsible for IS in Chinese populations. We analyzed data from eight studies that covered a total of 9756 individuals with Chinese ancestry comprising 4254 cases and 5502 c...
Source: International Journal of Immunopathology and Pharmacology - May 6, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Progress and prospects in the management of kidney stones and developments in phyto-therapeutic modalities.
This study was aimed to explore and elaborate the efficacy and availability of alternative treatment for kidney stones and to provide safe, cost effective, and efficacious management options as well as reduce the burden of disease. Cost effectiveness may enable improvements in treatment efficiency that can benefit patients and the healthcare system. Further randomized clinical trials should be conducted to evaluate the safety and efficacy of medicinal plants. PMID: 31046493 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - May 4, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research