MicroRNA-103 regulates the progression in endometrial carcinoma through ZO-1.
In conclusion, miR-103 could regulate EC cell proliferation through directly targeting ZO-1. Our results provide a potential development of microRNA-based targeted approaches for the treatment of EC. PMID: 31456452 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - August 30, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Green tea polyphenols protect PC12 cells against H2O2-induced damages by upregulating lncRNA MALAT1.
In this study, we aimed to investigate the protective effects of GTP against H2O2-caused injuries in PC12 cells as well as the molecular underpinnings associated with long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). PC12 cells were preincubated with GTP prior to H2O2 stimulation. Furthermore, MALAT1-deficient PC12 cells were constructed by transfection and identified by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Next, viability and apoptosis were detected by cell counting kit-8 and flow cytometry, respectively. Meanwhile, Western blot assay was carried out to monit...
Source: International Journal of Immunopathology and Pharmacology - August 30, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Efficacy and safety of eltrombopag in the treatment of severe chronic immune thrombocytopenia in children of China: A single-center observational study.
Authors: Cheng X, Yan K, Ma J, Chen Z, Zhao L, Wang X, Wu R Abstract The treatment of severe chronic immune thrombocytopenia (SCITP) in pediatric patients is challenging. We evaluated the clinical efficacy and safety of eltrombopag in children with SCITP in China. This observational study was carried out at the Hematology Oncology Center, Beijing Children's Hospital between April 2017 and July 2018. Patients with SCITP who had at least 12 weeks of eltrombopag treatment and follow-up data were included. Baseline data, such as age, drug dosage, pre-study platelet count, concomitant medications, and bleeding se...
Source: International Journal of Immunopathology and Pharmacology - August 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

The combination of Astragalus membranaceus extract and ligustrazine to improve the inflammation in rats with thrombolytic cerebral ischemia.
Authors: Pan R, Zhou M, Zhong Y, Xie J, Ling S, Tang X, Huang Y, Chen H Abstract The purpose of the study was to evaluate the effect of Astragalus membranaceus extract and ligustrazine combination on ameliorating inflammation in cerebral ischemic rats that have undergone thrombolysis. Astragalus membranaceus and ligustrazine per se, or a combination of A. membranaceus and ligustrazine was administered by intraperitoneal injection immediately after surgery and sham surgery. After the induction of thrombolysis, the neurological function was measured and cerebral lesion volume was determined. The regulatory T cells in...
Source: International Journal of Immunopathology and Pharmacology - August 16, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Inflammatory bowel disease: Looking beyond the tract.
Authors: Chams S, Badran R, Sayegh SE, Chams N, Shams A, Hajj Hussein I Abstract Inflammatory bowel disease is a chronic inflammatory condition that encompasses Crohn's disease and ulcerative colitis. Inflammatory bowel disease is not exclusive to the gastrointestinal system, as it has been identified to be associated with extraintestinal manifestations that encompass every other organ system in the human body. This review article will comprehensively review the current knowledge on extraintestinal manifestations of inflammatory bowel disease. In addition, it will discuss the recommendations for screening and surve...
Source: International Journal of Immunopathology and Pharmacology - August 7, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Protective effects of ginsenoside Rb1 on H2O2-induced oxidative injury in human endothelial cell line (EA.hy926) via miR-210.
Authors: Jia F, Mou L, Ge H Abstract Ginsenoside Rb1 (Rb1) possesses a cardioprotective effect via mediating microRNAs (miRs), while it is unexplored whether miR-210 is regulated by Rb1 in response to oxidative stress. Human endothelial EA.hy926 cells were stimulated with H2O2 before Rb1 treatment. After transfection, cell viability, apoptosis, migration, and invasion assays were conducted. Western blot was applied to quantify protein. BCL2/adenovirus E1B 19-kDa interacting protein 3 (BNIP3) and miR-210 were analyzed with quantitative reverse transcription polymerase chain reaction. Dual luciferase activity assay w...
Source: International Journal of Immunopathology and Pharmacology - August 1, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Berberine reduces neuroglia activation and inflammation in streptozotocin-induced diabetic mice.
Authors: Liu M, Gao L, Zhang N Abstract We aimed to analyze the action of berberine on the neuropathic pain and neuroglia activation in experimental diabetes mellitus (DM) model. Diabetes in mice was induced by intraperitoneal injection of streptozotocin (STZ) followed by the administration of berberine. Mechanical allodynia and thermal hyperalgesia and activations of microglia and astrocytes were evaluated. The levels of pro-inflammatory cytokines and protein expressions of inflammatory proteins were assessed by enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. Our results revealed the anti...
Source: International Journal of Immunopathology and Pharmacology - July 25, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effects of GIT-27NO, a NO-donating compound, on hepatic ischemia/reperfusion injury.
In this study, we have investigated the effects of this compound in vitro, in a model of oxidative stress induced in HepG2 cells by hydrogen peroxide (H2O2), and in vivo, in a rat model of IRI of the liver. GIT-27NO significantly counteracted the toxic effects induced by the H2O2 on the HepG2 cells and in vivo, GIT-27NO reduced the transaminase levels and the histological liver injury by reducing necrotic areas with preservation of viable tissue. These effects were almost similar to that of the positive control drug dimethyl fumarate. These data suggest that the beneficial effect of GIT-27NO in the hepatic IRI can be secon...
Source: International Journal of Immunopathology and Pharmacology - July 14, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Regulatory T-cells and their impacts on cytokine profile of end-stage renal disease patients suffering from systemic lupus erythematosus.
This study aimed to determine Treg frequency and its effects on cytokine profile of ESRD patients with and without systemic lupus erythematosus (SLE). Moreover, this study also determines how Treg number is affected by blood transfusion and gender. Peripheral blood mononuclear cells were isolated from 26 ESRD and 10 healthy subjects and stained with anti-CD4, anti-CD25, and anti-FoxP3 antibodies. Treg frequencies in ESRD patients with and without blood transfusion were determined by flow cytometry. Antibodies against human leukocyte antigens (HLAs) were investigated by panel-reactive antibodies screening. Tumor growth fact...
Source: International Journal of Immunopathology and Pharmacology - July 11, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Primary prostate lymphoma: A case report and literature review.
This article reports a case of PPL by retrospective analysis of the clinical data and review of the literature, in an attempt to explore the diagnosis, treatment, and prognosis of this rare disease. The present case involves a male patient who came to our hospital for medical consultation of dysuria. Serum prostate-specific antigen (PSA) was not remarkable. Pelvic computed tomography (CT) scan suggested obvious enlargement of the prostate and a tumor in the prostate, but the tumor was not significantly enhanced on contrast-enhanced CT scan. Ultrasound suggested obvious enlargement of the prostate with multiple local low-de...
Source: International Journal of Immunopathology and Pharmacology - July 11, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Curcumin inhibits the lymphangiogenesis of gastric cancer cells by inhibiton of HMGB1/VEGF-D signaling.
In this study, the cytotoxic effects of curcumin were investigated in gastric cancer AGS and SGC-7901 cell lines by MTT assay, and curcumin-induced morphological changes and cell apoptosis were assessed by using flow cytometry analysis and caspase-3 activity. The effects of curcumin on HMGB1 and VEGF-D expression were examined by reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis. As a result, we found that curcumin decreased cell viability and caused a dose-dependent cell apoptosis through the activation of caspase-3. The mRNA and protein expression levels of HMGB1 and VEGF-D were significa...
Source: International Journal of Immunopathology and Pharmacology - July 5, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Sulforaphane alleviates retinal ganglion cell death and inflammation by suppressing NLRP3 inflammasome activation in a rat model of retinal ischemia/reperfusion injury.
This study aims to study the potentials of sulforaphane (SFN) against retinal ischemia/reperfusion (I/R) injury. A rat retinal I/R injury method was established. Retinal thickness change and retinal ganglion cell (RGC) death were determined using hematoxylin and eosin (H&E) staining and Fluoro-Gold (FG) labeling. The inflammatory cytokines production and microglia activation were evaluated by using quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and enzyme-linked immunosorbent assay (ELISA). Knockdown NLRP3 was performed, and the according changes of retinal RGCs were assessed. SFN administrat...
Source: International Journal of Immunopathology and Pharmacology - July 5, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

The effect of perirenal puncture and drainage with urokinase treatment of perirenal hematoma complicated by infection: Two cases' report.
Authors: Li B, Li D, Liang T, Li X Abstract The aim of this study was to discuss the clinical significance of perirenal puncture and drainage with urokinase treatment of perirenal hematoma complicated by infection following surgery for upper urinary tract calculi. Two cases of perirenal hematoma complicated by infection following surgery for upper urinary tract calculi in 2017, and later received perirenal puncture and drainage of perirenal hematoma with urokinase treatment were selected. Puncture and drainage of perirenal hematoma with urokinase treatment were performed without the occurrence of severe complicatio...
Source: International Journal of Immunopathology and Pharmacology - June 28, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

LncRNA MALAT1 contributes to non-small cell lung cancer progression via modulating miR-200a-3p/programmed death-ligand 1 axis.
In conclusion, LncRNA MALAT1 facilitates the progression of NSCLC by modulating miR-200a-3p/PDL1 axis. PMID: 31240979 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - June 28, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effect of stress on learning and motivation-relevance to autism spectrum disorder.
Authors: Theoharides TC, Kavalioti M Abstract Learning and motivation are critical in the development of children, and to their acquisition of knowledge and skills. A case in point is autism spectrum disorder (ASD), a neurodevelopmental condition characterized by impaired social interactions and communication, as well as by stereotypic movements. Maternal stress has been strongly associated with increased risk of developing ASD. Children experience multiple stressors such as separation anxiety, fear of the unknown, physical and/or emotional trauma, bullying, as well as environmental exposures. Stress is well known ...
Source: International Journal of Immunopathology and Pharmacology - June 22, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Skullcapflavone I protects cardiomyocytes from hypoxia-caused injury through up-regulation of lincRNA-ROR.
This study explored the possible protective activity of Skullcapflavone I (SF I), a flavonoid isolated from the root of Scutellaria baicalensis Georgi, on hypoxia-stimulated cardiomyocytes cell injury in vitro. Viability and apoptosis of H9c2 cells and primary cardiomyocytes were tested using cell counting kit-8 (CCK-8) assay and Guava Nexin Reagent, respectively. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the long non-coding RNA regulator of reprogramming (lincRNA-ROR) expression. si-ROR was transfected to knockdown lincRNA-ROR. Western blotting was conducted to assess the p...
Source: International Journal of Immunopathology and Pharmacology - June 22, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Notoginsenoside R1 protects human keratinocytes HaCaT from LPS-induced inflammatory injury by downregulation of Myd88.
Authors: Zhang J, Zheng Q, Lu H, Jin F, Li Y, Bi F, Xu J Abstract Burn injury is a gigantic challenge in public health which brings multiple negative effects to patients both in physical and spiritual aspects. Inflammation plays vital roles in the progression of burn injury, and our study investigated whether notoginsenoside R1 (NGR1) alleviated lipopolysaccharide (LPS)-induced human keratinocyte HaCaT cell inflammatory injury. Inflammatory injury was induced by LPS in HaCaT cells. Stimulated cells were then treated by NGR1 in different concentrations. Cell viability and cell apoptosis were detected by Cell Countin...
Source: International Journal of Immunopathology and Pharmacology - June 18, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Searching for highly sensitive and specific biomarkers for sepsis: State-of-the-art in post-mortem diagnosis of sepsis through immunohistochemical analysis.
Authors: La Russa R, Maiese A, Viola RV, De Matteis A, Pinchi E, Frati P, Fineschi V Abstract The autoptical observations commonly ascribed to sepsis deal with unspecific general and local signs of inflammation or ischemia, such as myocardial inflammation, pulmonary edema and infiltration, cerebral swallowing, and tubular necrosis in the kidney. In the two last decades, some studies have been carried out to implement immunohistochemical markers for post-mortem diagnosis. All of these target molecules are specifically up-regulated or down-regulated during systemic inflammatory responses, especially for infective cau...
Source: International Journal of Immunopathology and Pharmacology - June 11, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Genetic variants on chromosome 9p21 confer risks of cerebral infarction in the Chinese population: a meta-analysis.
Authors: Chen JX, Liu J, Hu F, Bi Y, Li M, Zhao L Abstract Ischemic stroke (IS) is the leading cause of disability. Researchers have demonstrated that IS is more a multifactorial disorder than a single-factor disease. At present, no consistent conclusions have been reached on susceptibility loci for IS on chromosome 9p21. We conducted this meta-analysis to verify whether genetic loci on chromosome 9p21 reported domestically and abroad could be responsible for IS in Chinese populations. We analyzed data from eight studies that covered a total of 9756 individuals with Chinese ancestry comprising 4254 cases and 5502 c...
Source: International Journal of Immunopathology and Pharmacology - May 6, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Progress and prospects in the management of kidney stones and developments in phyto-therapeutic modalities.
This study was aimed to explore and elaborate the efficacy and availability of alternative treatment for kidney stones and to provide safe, cost effective, and efficacious management options as well as reduce the burden of disease. Cost effectiveness may enable improvements in treatment efficiency that can benefit patients and the healthcare system. Further randomized clinical trials should be conducted to evaluate the safety and efficacy of medicinal plants. PMID: 31046493 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - May 4, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Beyond appearance: An unusual manifestation of isolated oral secondary syphilis.
We report a case of isolated oral manifestation as the unique presentation of secondary syphilis. PMID: 31020874 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - April 26, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Using cytometric bead arrays to detect cytokines in the serum of patients with different types of pulmonary tuberculosis.
Authors: Bai R, Tao L, Li B, Liu A, Dai X, Ji Z, Jian M, Ding Z, Luo L, Chen T, Ma M, Peng Y, Bao F Abstract Cytokines play a crucial role in mediating immune responses to tuberculosis (TB). The aim of this study was to evaluate the levels of cytokines in patients with different forms of pulmonary tuberculosis (PTB) and identify valuable cytokine biomarkers for the diagnosis of PTB. We measured the levels of six cytokines (interleukin (IL-2, IL-4, IL-6, and IL-10), tumor necrosis factor (TNF-α), and interferon-γ (IFN-γ)) in the serum of healthy donors (n = 30). Patients with active P...
Source: International Journal of Immunopathology and Pharmacology - April 25, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Potential role of MRN-100, an iron-based compound, in upregulating production of cytokine IL-10 in human dendritic cells to promote an anti-inflammatory response in vitro.
Authors: Ghoneum MH, Gimzewski JK, Ghoneum AD, Agrawal S Abstract The hydroferrate fluid MRN-100, an iron-based compound with potent antioxidant characteristics, was examined to identify its possible anti-inflammatory effects on human dendritic cells (DCs) in vitro. Human monocyte-derived DCs were treated with MRN-100 at two concentrations (50 and 100 μL/mL) for 24 h and then stimulated with or without lipopolysaccharides (LPS). The expression of DC maturation markers was assessed by flow cytometry and the production of cytokines was determined by enzyme-linked immunosorbent assay (ELISA). Function...
Source: International Journal of Immunopathology and Pharmacology - April 19, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

MiR-516a-5p inhibits the proliferation of non-small cell lung cancer by targeting HIST3H2A.
Authors: Ye XY, Xu L, Lu S, Chen ZW Abstract The dysregulation of microRNAs (miRNAs) is associated with the pathogenesis of non-small cell lung cancer (NSCLC). However, the mechanisms by which miR-516a-5p contributes to NSCLC remain unclear. The association between miR-516a-5p expression and the clinicopathological characteristics and prognosis in patients with NSCLC was analyzed by The Cancer Genome Atlas (TCGA) data set. The targets of miR-516a-5p were identified by bioinformatic analysis and luciferase report assay. MTT and soft agar assays were conducted to investigate the function of miR-516a-5p in NSCLC cells...
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Lifelong immunoglobulin replacement is not always necessary: A case description of a patient with recurrent infections and hypogammaglobulinemia.
Authors: Napiorkowska-Baran K, Janicki R, Koltan S, Szynkiewicz E, Bartuzi Z Abstract Humoral immunodeficiency with accompanying infections is an indication for human immunoglobulin replacement therapy. Whether treatment will be lifelong or necessary only temporarily depends on the nature of deficiency: primary (persistent) or secondary (persistent or transient). It is not always easy to distinguish between primary and secondary immunodeficiency, especially in adults. The article presents a case of a 39-year-old patient with anamnesis and medical tests results that suggested primary humoral immunodeficiency. The de...
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Immunomodulatory effects of chemotherapy on blood lymphocytes and survival of patients with advanced non-small cell lung cancer.
In conclusion, the imbalance of lymphocyte subsets is a hallmark of NSCLC. Furthermore, the high level of PD-1+CD4+ cells plays a crucial role in the progression of NSCLC and could be used as a prognostic marker; and the high level of Th1 could predict better clinical outcomes of chemotherapy. PMID: 30968711 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Common variable immunodeficiency patients display elevated plasma levels of granulocyte activation markers elastase and myeloperoxidase.
Authors: Litzman J, Chovancová Z, Bejdák P, Litzman M, Hel Z, Vlková M Abstract Common variable immunodeficiency disorders (CVIDs) represent a group of primary immunodeficiency diseases characterized by hypogammaglobulinemia and dysfunctional immune response to invading pathogens. Previous studies have indicated that CVID is associated with microbial translocation and systemic myeloid cell activation. The goal of this study was to determine whether patients with CVID display elevated systemic levels of markers of granulocyte activation and whether the levels are further influenced by intravenou...
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

PCC0208018 exerts antitumor effects by activating effector T cells.
This study showed that PCC0208018 potentially increased PBMCs proliferation and function by activating the phosphatidylinositol 3 kinase (PI3K)/AKT and mitogen-activated protein kinase (MEK)/ERK pathways to exert antitumor effects. PMID: 30968715 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Is dextran sulfate sodium a good inducer of acute experimental enteritis?
In conclusion, oral administration of 3%-5% DSS is a good inducer of AEC models, but not AEE models. PMID: 30968717 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Immunotherapy in gliomas: Are we reckoning without the innate immunity?
Authors: Vismara MFM, Donato A, Malara N, Presta I, Donato G Abstract Innate immunity plays a central role in neoplasms, including those affecting the central nervous system (CNS). Nowadays, tumors classification, especially that regarding gliomas, is based on molecular features such as mutations in isocitrate dehydrogenase (IDH) genes and the presence of co-deletion 1p/19q. Therapy, in most cases, is based on surgery, radiotherapy, and pharmacological treatment with chemotherapeutic agents such as temozolomide. However, the results of the treatments, after many decades, are not completely satisfactory. There is a ...
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Autoimmune thyroid disease following treatment with alemtuzumab for multiple sclerosis.
We report here a case of alemtuzumab-induced thyroid disease in a female patient who showed a phase of thyrotoxicosis with positive anti-thyroglobulin (anti-Tg) and anti-thyroid peroxidase (TPO) antibodies, but a negative TSH receptor antibody, spontaneously followed by hypothyroidism. The aim is to illustrate the clinical presentation, evaluation over time, and the possibility to consider a conservative management up to the spontaneous resolution of the thyrotoxicosis. All these are intended to emphasize the importance of pretreatment screening and follow-up in the management of treatment with alemtuzumab. PMID: 30968...
Source: International Journal of Immunopathology and Pharmacology - April 12, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effect of Chinese herbal compound GAPT on the early brain glucose metabolism of APP/PS1 transgenic mice.
Authors: Mana L, Feng H, Dong Y, Wang Y, Shi J, Tian J, Wang P Abstract A number of studies have shown that early-stage Alzheimer's disease (AD) is associated with abnormal brain glucose metabolism before cognitive decline, which may be the key pathological change of asymptomatic AD. The pathogenesis of AD in traditional Chinese medicine is kidney deficiency and turbid phlegm. Based on this, GAPT (a mixture of herbal extracts) was made to invigorate kidney Yang and eliminate phlegm. Previous studies have shown that GAPT can improve and delay the memory decline, but the specific therapeutic target of AD in an early ...
Source: International Journal of Immunopathology and Pharmacology - April 9, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

CD3, CD4, CD8, CD19 and CD16/CD56 positive cells in tuberculosis infection and disease: Peculiar features in children.
Authors: Venturini E, Lodi L, Francolino I, Ricci S, Chiappini E, de Martino M, Galli L Abstract Pathogenesis of mycobacterial infection has been extensively studied determining the fundamental role of host immunocompetence in disease progression. Cellular adaptive immunity, in particular CD4+ cells, has shown to be crucial in the host defence. A role of cytotoxic lymphocytes and humoral immunity has also been established. However, few studies have been performed in low endemic countries on immunological correlates of tuberculosis in paediatric patients. The present study aims to fill this gap analysing the distrib...
Source: International Journal of Immunopathology and Pharmacology - April 9, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Levels and effectiveness of oral retinol supplementation in VLBW preterm infants.
Authors: Garofoli F, Mazzucchelli I, Decembrino L, Bartoli A, Angelini M, Broglia M, Tinelli C, Banderali G, Stronati M Abstract Retinol palmitate oral administration is convenient, but it is difficult to assess/monitor its nutritional status in preterm infants and literature is controversial about the administration route and the effectiveness of vitamin A supplementation. We primarily evaluated retinol plasma levels to assess the vitamin A nutritional status in preterm infants (
Source: International Journal of Immunopathology and Pharmacology - March 23, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

The neuropathic pain: An overview of the current treatment and future therapeutic approaches.
Authors: Cavalli E, Mammana S, Nicoletti F, Bramanti P, Mazzon E Abstract Neuropathic pain is characterized by abnormal hypersensitivity to stimuli (hyperalgesia) and nociceptive responses to non-noxious stimuli (allodynia). The conditions and the pathophysiological states that determine the onset of neuropathic pain are heterogeneous, such as metabolic disorders, neuropathy caused by viral infections, and autoimmune diseases affecting the central nervous system (CNS). Neuropathic pain in the general population is estimated to have a prevalence ranging between 3% and 17%. Most of the available treatments for neurop...
Source: International Journal of Immunopathology and Pharmacology - March 23, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Metformin inhibits both oleic acid-induced and CB1R receptor agonist-induced lipid accumulation in Hep3B cells: The preliminary report.
Authors: Szuster-Ciesielska A, Zwolak A, Daniluk J, Kandefer-Szerszeń M Abstract Fatty liver is characterized by excessive accumulation of triglycerides within hepatocytes. Recent findings indicate that natural history of nonalcoholic fatty liver is regulated, in part, by endogenous cannabinoids. Metformin is an oral hypoglycemic medication which inhibits gluconeogenesis and glycogenolysis in hepatocytes and limits lipid storage in the liver through the inhibition of free fatty acid formation via induction of activated protein kinase activity (AMPK). Both endocannabinoids and metformin may modulate hepatosteatosis...
Source: International Journal of Immunopathology and Pharmacology - March 20, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Vitamin D serum levels in children with vernal keratoconjunctivitis and disease control.
Authors: Ghiglioni DG, Bruschi G, Gandini S, Osnaghi S, Peroni D, Marchisio P Abstract The aim of this study was to evaluate whether the control of ocular symptoms with cyclosporine or with tacrolimus in eye drops allows to improve sun exposure and therefore serum level of vitamin D (VD; 25OHD), in the more severe forms of vernal keratoconjunctivitis (VKC). Out of 242 children followed for active VKC, 94 were treated with 1% cyclosporine or 0.1% tacrolimus eye drops, while the other 148 with mild VKC did not need to be treated with immunomodulators. VD serum levels were measured in spring and autumn in 71 children....
Source: International Journal of Immunopathology and Pharmacology - March 9, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Eosinophilic oesophagitis (in nickel-allergic patient) regressed after nickel oral desensitization: A case report.
We describe the case of a 48-year-old woman suffering from EoE not responsive to the topical steroid administration and diagnosis of SNAS. The patient started nickel oral desensitization according to the literature protocol continuing nickel-free diet. After 1 year from the beginning of the treatment, during the maintenance dose (500 ng three times a week), she decreased gradually the dosage of immunotherapy and reintroduced all the culprit foods. After the immunotherapy interruption, during the free diet, she repeated the oesophagogastroscopy with a complete macroscopic and histological resolution. We showed...
Source: International Journal of Immunopathology and Pharmacology - March 6, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effect of voluntary running on expression of myokines in brains of rats with depression.
This study aimed to demonstrate the histopathology and immunoexpression of exercise-derived myokines in dentate gyrus (DG), medial prefrontal cortex (mPFC) and cerebellum of depressed Wistar rats during depression and after practising voluntary running. Depression was developed by forced swimming for 2 weeks. Voluntary running was performed by voluntary running for 3 weeks. Brain sections were processed and immunostained to detect brain-derived neurotrophic factor (BDNF), macrophage migration inhibitory factor (MIF), vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6). ImageJ software was used ...
Source: International Journal of Immunopathology and Pharmacology - March 6, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Tripterine up-regulates miR-223 to alleviate lipopolysaccharide-induced damage in murine chondrogenic ATDC5 cells.
This study revealed that tripterine protected ATDC5 cells against LPS-induced cell damage possibly via up-regulation of miR-223 and modulation of NF-κB and PI3K/AKT pathways. PMID: 30791741 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effects of 12-months treatment with zoledronate or teriparatide on intima-media thickness of carotid artery in women with postmenopausal osteoporosis: A pilot study.
In conclusion, ZLN and TPT treatments are safe on carotid walls in osteoporotic women with subclinical atherosclerosis; circulating OPG and OPN are not affected by long-term anti-osteoporotic treatments and do not correlate with CA-IMT. PMID: 30791743 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Possible treatment for cutaneous lichen planus: An in vitro anti-inflammatory role of Angelica polysaccharide in human keratinocytes HaCaT.
In conclusion, AP could alleviate LPS-induced inflammatory injury of HaCaT cells through upregulating SIRT1 expression and then activating Nrf2/HO-1 pathway but inactivating NF-κB pathway. This study provided a possible therapeutic strategy for clinical CLP treatments. PMID: 30791744 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Blocking of checkpoint receptor PD-L1 aggravates osteoarthritis in macrophage-dependent manner in the mice model.
Authors: Liu S, Mi J, Liu W, Xiao S, Gao C Abstract As a chronic degenerative joint disease, osteoarthritis is among the most common diseases all over the world. In osteoarthritis, inflammation plays an important role in the generation of joint symptoms and the development of disease. When the programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) immune checkpoint is blocked, the antitumor immunity will be enhanced. We aim to illustrate the function of PD-L1 in osteoarthritis. Osteoarthritis in mice was induced by the injection of collagenase or anterior cruciate ligament transection (ACLT). Anti-PD-L1 w...
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Changes in the transcriptome of circulating immune cells of a New Zealand cohort with myalgic encephalomyelitis/chronic fatigue syndrome.
Authors: Sweetman E, Ryan M, Edgar C, MacKay A, Vallings R, Tate W Abstract Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a poorly understood disease affecting 0.2%-2% of the global population. To gain insight into the pathophysiology of ME/CFS in New Zealand, we examined the transcriptomes of peripheral blood mononuclear cells by RNA-seq analysis in a small well-characterized patient group (10 patients), with age/gender-matched healthy controls (10 control subjects). Twenty-seven gene transcripts were increased 1.5- to sixfold and six decreased three- to sixfold in the patient group ( P 
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Reuterinos ® as adjuvant for peri-implant treatment: A pilot study.
The objective of this study was to evaluate the effects of lozenges-containing Lactobacillus reuteri as an adjuvant treatment of peri-implant mucositis and to detect the level of L. reuteri colonization in the peri-implant tissues of treated patients. A total of 10 patients were selected. Subjects with at least one implant affected by peri-implant mucositis, with gingival index (GI) of ⩾2 in each quadrant, evaluated at the buccal aspect of all teeth. Patients included in the study were partially edentulous and had implants with mucositis or peri-implantitis. Implants with radiographic bone loss of ⩾5 mm and/or ...
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Detection of Mycobacterium tuberculosis in AFB smear-negative sputum specimens through MTB culture and GeneXpert ® MTB/RIF assay.
This study was undertaken to evaluate the performance of GeneXpert® MTB/RIF assay and MTB culture for the detection of Mycobacterium tuberculosis (MTB) in sputum smear-negative pulmonary TB/drug-resistant tuberculosis (DR-TB) suspects. A total of 168 sputum smear-negative TB suspects were recruited for the study. Among the suspected TB cases, 52.98% were male and 47.02% were females with the mean age of 42 ± 17.6 years. All the sputum specimens collected from the study population were subjected to Ziehl-Neelsen (ZN) smear microscopy, GeneXpert MTB/RIF assay, and MTB culture. The results revealed that, out...
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Antifungal activity in the methanolic, aqueous and hexane extracts of Calligonum polygonoides.
Authors: Ahmad S, Akram M Abstract Calligonum polygonoides is locally called as Phog which belongs to the Polygonaceae family. It is traditionally used as an antifungal. The methanolic extract, hexane extract, ethyl acetate extract and aqueous extract were screened against Candida albicans and Aspergillus niger in seven concentrations, that is, 1.8, 2.9, 6.5, 12.6, 25, 50 and 75 µg/mL/disc. Calligonum polygonoides showed significant activity against Candida albicans as the observed minimum inhibitory concentration (MIC) is 6.5 µg/mL for methanolic extract, 9.8 µg/mL for ethyl acetate extract, wher...
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

LncRNA SNHG3 promotes cell growth by sponging miR-196a-5p and indicates the poor survival in osteosarcoma.
In conclusion, lncRNA SNHG3 promoted cell growth by sponging miR-196a-5p and indicated a poor prognosis in OS patients. PMID: 30791797 [PubMed - in process] (Source: International Journal of Immunopathology and Pharmacology)
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Effect of short peptides on neuronal differentiation of stem cells.
Authors: Caputi S, Trubiani O, Sinjari B, Trofimova S, Diomede F, Linkova N, Diatlova A, Khavinson V Abstract It has been demonstrated that short peptides play an important role in the transmission of biological information, modulation of transcription, and restoring genetically conditioned alterations occurring with age. Peptidergic regulation of homeostasis occupies an important place in physiological processes, which lead to the aging of cells, tissues, and organs, consisting in the involution of major regulatory systems-the nervous, the endocrine, and the immune. The effect of AED (Ala-Glu-Asp), KED (Lys-Glu-As...
Source: International Journal of Immunopathology and Pharmacology - February 24, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research

Heat stress combined with lipopolysaccharide alter the activity and superficial molecules of peripheral monocytes.
The objectives were divided into four groups: 43°C heat stress combined with lipopolysaccharide (LPS) group, 43°C heat stress group, LPS group, and control group. There were 10 cases in each group. An enzyme-linked immunosorbent assay (ELISA) test was used to measure the concentrations of supernatant inflammatory mediators (tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-10 (IL-10)). After loaded by 2,7-Dichlorodi-hydrofluorescein-diacetate (DCFHDA) fluorescent probe, intracellular reactive oxygen species (ROS) levels were determined by a flow cytometry. After fluorescent...
Source: International Journal of Immunopathology and Pharmacology - February 17, 2019 Category: Allergy & Immunology Tags: Int J Immunopathol Pharmacol Source Type: research