Dual Drug Nanoparticle Synergistically Induced Apoptosis, Suppressed Inflammation and Protected Autophagic Response in Rheumatoid Arthritis Fibroblast-like Synoviocytes
In this study, we assessed the therapeutic effectiveness of DD NP HG in primary RA-FLS cells isolated from the synovial tissue of FCA-induced RA rats. We observed that DD NP HG had good biosafety for healthy FLS cells and, at higher concentrations, a mild inhibitory effect on RA-FLS. The combination therapy (DD NP HG) of MTX NP and PEITC NE in RA-FLS showed a higher rate of apoptosis with significantly reduced LPS-induced expression of pro-inflammatory cytokines (TNF-α, IL-17A, and IL-6) in arthritic FLSs. Further, the gene expression studies showed that DD NP HG significantly down-regulated the mRNA expression of IL-1β,...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Prakash Haloi Rajat Choudhary B Siva Lokesh V Badireenath Konkimalla Source Type: research
Endoplasmic reticulum stress response in immune cells contributes to experimental autoimmune encephalomyelitis pathogenesis in rats
We examined the role of endoplasmic reticulum (ER) stress and the ensuing unfolded protein response (UPR) in the development of the central nervous system (CNS)-directed immune response in the rat model of experimental autoimmune encephalomyelitis (EAE). The induction of EAE with syngeneic spinal cord homogenate in complete Freund's adjuvant (CFA) caused a time-dependent increase in the expression of ER stress/UPR markers glucose-regulated protein 78 (GRP78), X-box-binding protein 1 (XBP1), C/EBP homologous protein (CHOP), and phosphorylated eukaryotic initiation factor 2α (eIF2α) in the draining lymph nodes of both EAE-...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Sasenka Vidicevic Jelena Tasic Zeljka Stanojevic Darko Ciric Tamara Martinovic Verica Paunovic Sasa Petricevic Nina Tomonjic Aleksandra Isakovic Vladimir Trajkovic Source Type: research
Conventional dendritic cells 2, the targeted antigen-presenting-cell, induces enhanced type 1 immune responses in mice immunized with CVC1302 in oil formulation cDC2 responsible for type 1 immunity induced by CVC1302
Immunol Lett. 2024 Mar 25:106856. doi: 10.1016/j.imlet.2024.106856. Online ahead of print.ABSTRACTMultifunctional CD4+ T helper 1 (Th1) cells, producing IFN-γ, TNF-α and IL-2, define a correlate of vaccine-mediated protection against intracellular infection. In our previous study, we found that CVC1302 in oil formulation promoted the differentiation of IFN-γ+/TNF-α+/IL-2+ Th1 cells. In order to extend the application of CVC1302 in oil formulation, this study aimed to elucidate the mechanism of action in improving the Th1 immune response. Considering the signals required for the differentiation of CD4+ T cells to Th1 ce...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Luping Du Haiyan Lu Xuwen Qiao Yuanpeng Zhang Liting Hou Xiaoming Yu Haiwei Cheng Jin Chen Qisheng Zheng Jibo Hou Guangzhi Tong Source Type: research
Dual Drug Nanoparticle Synergistically Induced Apoptosis, Suppressed Inflammation and Protected Autophagic Response in Rheumatoid Arthritis Fibroblast-like Synoviocytes
In this study, we assessed the therapeutic effectiveness of DD NP HG in primary RA-FLS cells isolated from the synovial tissue of FCA-induced RA rats. We observed that DD NP HG had good biosafety for healthy FLS cells and, at higher concentrations, a mild inhibitory effect on RA-FLS. The combination therapy (DD NP HG) of MTX NP and PEITC NE in RA-FLS showed a higher rate of apoptosis with significantly reduced LPS-induced expression of pro-inflammatory cytokines (TNF-α, IL-17A, and IL-6) in arthritic FLSs. Further, the gene expression studies showed that DD NP HG significantly down-regulated the mRNA expression of IL-1β,...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Prakash Haloi Rajat Choudhary B Siva Lokesh V Badireenath Konkimalla Source Type: research
Endoplasmic reticulum stress response in immune cells contributes to experimental autoimmune encephalomyelitis pathogenesis in rats
We examined the role of endoplasmic reticulum (ER) stress and the ensuing unfolded protein response (UPR) in the development of the central nervous system (CNS)-directed immune response in the rat model of experimental autoimmune encephalomyelitis (EAE). The induction of EAE with syngeneic spinal cord homogenate in complete Freund's adjuvant (CFA) caused a time-dependent increase in the expression of ER stress/UPR markers glucose-regulated protein 78 (GRP78), X-box-binding protein 1 (XBP1), C/EBP homologous protein (CHOP), and phosphorylated eukaryotic initiation factor 2α (eIF2α) in the draining lymph nodes of both EAE-...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Sasenka Vidicevic Jelena Tasic Zeljka Stanojevic Darko Ciric Tamara Martinovic Verica Paunovic Sasa Petricevic Nina Tomonjic Aleksandra Isakovic Vladimir Trajkovic Source Type: research
Conventional dendritic cells 2, the targeted antigen-presenting-cell, induces enhanced type 1 immune responses in mice immunized with CVC1302 in oil formulation cDC2 responsible for type 1 immunity induced by CVC1302
Immunol Lett. 2024 Mar 25:106856. doi: 10.1016/j.imlet.2024.106856. Online ahead of print.ABSTRACTMultifunctional CD4+ T helper 1 (Th1) cells, producing IFN-γ, TNF-α and IL-2, define a correlate of vaccine-mediated protection against intracellular infection. In our previous study, we found that CVC1302 in oil formulation promoted the differentiation of IFN-γ+/TNF-α+/IL-2+ Th1 cells. In order to extend the application of CVC1302 in oil formulation, this study aimed to elucidate the mechanism of action in improving the Th1 immune response. Considering the signals required for the differentiation of CD4+ T cells to Th1 ce...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Luping Du Haiyan Lu Xuwen Qiao Yuanpeng Zhang Liting Hou Xiaoming Yu Haiwei Cheng Jin Chen Qisheng Zheng Jibo Hou Guangzhi Tong Source Type: research
Dual Drug Nanoparticle Synergistically Induced Apoptosis, Suppressed Inflammation and Protected Autophagic Response in Rheumatoid Arthritis Fibroblast-like Synoviocytes
In this study, we assessed the therapeutic effectiveness of DD NP HG in primary RA-FLS cells isolated from the synovial tissue of FCA-induced RA rats. We observed that DD NP HG had good biosafety for healthy FLS cells and, at higher concentrations, a mild inhibitory effect on RA-FLS. The combination therapy (DD NP HG) of MTX NP and PEITC NE in RA-FLS showed a higher rate of apoptosis with significantly reduced LPS-induced expression of pro-inflammatory cytokines (TNF-α, IL-17A, and IL-6) in arthritic FLSs. Further, the gene expression studies showed that DD NP HG significantly down-regulated the mRNA expression of IL-1β,...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Prakash Haloi Rajat Choudhary B Siva Lokesh V Badireenath Konkimalla Source Type: research
Endoplasmic reticulum stress response in immune cells contributes to experimental autoimmune encephalomyelitis pathogenesis in rats
We examined the role of endoplasmic reticulum (ER) stress and the ensuing unfolded protein response (UPR) in the development of the central nervous system (CNS)-directed immune response in the rat model of experimental autoimmune encephalomyelitis (EAE). The induction of EAE with syngeneic spinal cord homogenate in complete Freund's adjuvant (CFA) caused a time-dependent increase in the expression of ER stress/UPR markers glucose-regulated protein 78 (GRP78), X-box-binding protein 1 (XBP1), C/EBP homologous protein (CHOP), and phosphorylated eukaryotic initiation factor 2α (eIF2α) in the draining lymph nodes of both EAE-...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Sasenka Vidicevic Jelena Tasic Zeljka Stanojevic Darko Ciric Tamara Martinovic Verica Paunovic Sasa Petricevic Nina Tomonjic Aleksandra Isakovic Vladimir Trajkovic Source Type: research
Conventional dendritic cells 2, the targeted antigen-presenting-cell, induces enhanced type 1 immune responses in mice immunized with CVC1302 in oil formulation cDC2 responsible for type 1 immunity induced by CVC1302
Immunol Lett. 2024 Mar 25:106856. doi: 10.1016/j.imlet.2024.106856. Online ahead of print.ABSTRACTMultifunctional CD4+ T helper 1 (Th1) cells, producing IFN-γ, TNF-α and IL-2, define a correlate of vaccine-mediated protection against intracellular infection. In our previous study, we found that CVC1302 in oil formulation promoted the differentiation of IFN-γ+/TNF-α+/IL-2+ Th1 cells. In order to extend the application of CVC1302 in oil formulation, this study aimed to elucidate the mechanism of action in improving the Th1 immune response. Considering the signals required for the differentiation of CD4+ T cells to Th1 ce...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Luping Du Haiyan Lu Xuwen Qiao Yuanpeng Zhang Liting Hou Xiaoming Yu Haiwei Cheng Jin Chen Qisheng Zheng Jibo Hou Guangzhi Tong Source Type: research
Dual Drug Nanoparticle Synergistically Induced Apoptosis, Suppressed Inflammation and Protected Autophagic Response in Rheumatoid Arthritis Fibroblast-like Synoviocytes
In this study, we assessed the therapeutic effectiveness of DD NP HG in primary RA-FLS cells isolated from the synovial tissue of FCA-induced RA rats. We observed that DD NP HG had good biosafety for healthy FLS cells and, at higher concentrations, a mild inhibitory effect on RA-FLS. The combination therapy (DD NP HG) of MTX NP and PEITC NE in RA-FLS showed a higher rate of apoptosis with significantly reduced LPS-induced expression of pro-inflammatory cytokines (TNF-α, IL-17A, and IL-6) in arthritic FLSs. Further, the gene expression studies showed that DD NP HG significantly down-regulated the mRNA expression of IL-1β,...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Prakash Haloi Rajat Choudhary B Siva Lokesh V Badireenath Konkimalla Source Type: research
Endoplasmic reticulum stress response in immune cells contributes to experimental autoimmune encephalomyelitis pathogenesis in rats
We examined the role of endoplasmic reticulum (ER) stress and the ensuing unfolded protein response (UPR) in the development of the central nervous system (CNS)-directed immune response in the rat model of experimental autoimmune encephalomyelitis (EAE). The induction of EAE with syngeneic spinal cord homogenate in complete Freund's adjuvant (CFA) caused a time-dependent increase in the expression of ER stress/UPR markers glucose-regulated protein 78 (GRP78), X-box-binding protein 1 (XBP1), C/EBP homologous protein (CHOP), and phosphorylated eukaryotic initiation factor 2α (eIF2α) in the draining lymph nodes of both EAE-...
Source: Immunology Letters - March 27, 2024 Category: Allergy & Immunology Authors: Sasenka Vidicevic Jelena Tasic Zeljka Stanojevic Darko Ciric Tamara Martinovic Verica Paunovic Sasa Petricevic Nina Tomonjic Aleksandra Isakovic Vladimir Trajkovic Source Type: research
Corrigendum to'Aged A βPPswe/PS1ΔE9 mice as a useful animal model for studying the link between immunological senescence and diseases'[Immunol Lett. 2024 Feb 12:266:106842]
Immunol Lett. 2024 Mar 22:106850. doi: 10.1016/j.imlet.2024.106850. Online ahead of print.NO ABSTRACTPMID:38521725 | DOI:10.1016/j.imlet.2024.106850 (Source: Immunology Letters)
Source: Immunology Letters - March 23, 2024 Category: Allergy & Immunology Authors: Jing Luan Na Guo Fengrui Hu Xingchun Gou Lixian Xu Source Type: research
Corrigendum to'Aged A βPPswe/PS1ΔE9 mice as a useful animal model for studying the link between immunological senescence and diseases'[Immunol Lett. 2024 Feb 12:266:106842]
Immunol Lett. 2024 Mar 22:106850. doi: 10.1016/j.imlet.2024.106850. Online ahead of print.NO ABSTRACTPMID:38521725 | DOI:10.1016/j.imlet.2024.106850 (Source: Immunology Letters)
Source: Immunology Letters - March 23, 2024 Category: Allergy & Immunology Authors: Jing Luan Na Guo Fengrui Hu Xingchun Gou Lixian Xu Source Type: research
Corrigendum to'Aged A βPPswe/PS1ΔE9 mice as a useful animal model for studying the link between immunological senescence and diseases'[Immunol Lett. 2024 Feb 12:266:106842]
Immunol Lett. 2024 Mar 22:106850. doi: 10.1016/j.imlet.2024.106850. Online ahead of print.NO ABSTRACTPMID:38521725 | DOI:10.1016/j.imlet.2024.106850 (Source: Immunology Letters)
Source: Immunology Letters - March 23, 2024 Category: Allergy & Immunology Authors: Jing Luan Na Guo Fengrui Hu Xingchun Gou Lixian Xu Source Type: research
Corrigendum to'Aged A βPPswe/PS1ΔE9 mice as a useful animal model for studying the link between immunological senescence and diseases'[Immunol Lett. 2024 Feb 12:266:106842]
Immunol Lett. 2024 Mar 22:106850. doi: 10.1016/j.imlet.2024.106850. Online ahead of print.NO ABSTRACTPMID:38521725 | DOI:10.1016/j.imlet.2024.106850 (Source: Immunology Letters)
Source: Immunology Letters - March 23, 2024 Category: Allergy & Immunology Authors: Jing Luan Na Guo Fengrui Hu Xingchun Gou Lixian Xu Source Type: research
