Delphinidin diminishes in vitro interferon- γ and interleukin-17 producing cells in patients with psoriatic disease
AbstractThe anthocyanidin delphinidin reduces psoriasiform lesions and inflammatory mediators in human cell culture systems. Its role in psoriatic disease has not yet been investigated. We assessed delphinidin ’s in vitro immunomodulatory effect on ex vivo stimulated peripheral blood mononuclear cells (PBMCs) from 50 individuals [26 with psoriasis, 10 with psoriatic arthritis (PsA) and 14 healthy controls (HCs)]. Cells were either left untreated or stimulated with PMA plus ionomycin in the presence or a bsence of delphinidin. Intracellular production of interferon-γ (IFNγ), interleukin-17A (IL-17A), and i...
Source: Immunologic Research - November 25, 2021 Category: Allergy & Immunology Source Type: research

Hypoxia modulates human mast cell adhesion to hyaluronic acid
AbstractHypoxia is an inherent factor in the inflammatory process and is important in the regulation of some immune cell functions, including the expression of mast cell pro- and anti-inflammatory mediators. Hypoxia also influences cell adhesion to the extracellular matrix (ECM). Hyaluronic acid is one of the major components of the ECM that is involved in inflammatory and tissue regeneration processes in which mast cells play a prominent role. This prompted us to investigate the effects of hypoxia on the expression of hyaluronic acid receptors in mast cells and mast cell adhesion to this ECM component. We found that human...
Source: Immunologic Research - November 17, 2021 Category: Allergy & Immunology Source Type: research

Comparative structural analyses of selected spike protein-RBD mutations in SARS-CoV-2 lineages
AbstractThe severity of COVID-19 has been observed throughout the world as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) globally claimed more than 2 million lives and left a devastating impact worldwide. Recently several virulent mutant strains of this virus, such as the B.1.1.7, B.1.351, and P1 lineages, have emerged with initial predominance in UK, South Africa, and Brazil. Another extremely pathogenic B.1.617 lineage and its sub-lineages, first detected in India, are now affecting some countries at notably stronger spread-rates. The present paper computationally examines the time-based structures of B.1....
Source: Immunologic Research - November 16, 2021 Category: Allergy & Immunology Source Type: research

Analysis of inflammation-related microRNA expression in patients with ankylosing spondylitis
This study investigated the clinical diagnostic value of miR-146a, miR-15a, miR-20a, miR-125a-3p, miR-125a-5p, miR-125b-5p, miR-148a, miR-149a, miR-499, and miR-155a in AS. A total of 44 AS patients and 56 healthy controls (HCs) were included in the study. MiRNA expression levels were detected using fluorescence quantitative PCR (qPCR). Results showed that the expression levels of miR-146a, miR-125a-3p, miR-125a-5p, miR-125b-5p, and miR-155a decreased, whereas miR-499a expression increased significantly in AS patients compared to that in the controls. Logistic regression analysis with receiver operating characteristic (ROC...
Source: Immunologic Research - November 6, 2021 Category: Allergy & Immunology Source Type: research

A short focus, azithromycin in the treatment of respiratory viral infection COVID-19: efficacy or inefficacy?
AbstractAzithromycin is a macrolide antibiotic. Recent evidence has demonstrated in vitro activity against a wide variety of respiratory tract viruses, including SARS-CoV-2 responsible for the current global pandemic COVID-19. A mechanism of action acting on different phases of the viral cycle is assumed. In addition to its in vitro antiviral properties, some evidence also suggests immunomodulatory and antifibrotic activity. These properties of azithromycin could be useful in the treatment of viral respiratory tract infections such as COVID-19. However, clinical data on the antiviral efficacy of azithromycin in the treatme...
Source: Immunologic Research - November 5, 2021 Category: Allergy & Immunology Source Type: research

Host miRNA and immune cell interactions: relevance in nano-therapeutics for human health
AbstractAround 2200 miRNA (microRNA) genes were found in the human genome. miRNAs are arranged in clusters within the genome and share the same transcriptional regulatory units. It has been revealed that approximately 50% of miRNAs elucidated in the genome are transcribed from non-protein-coding genes, and the leftover miRNAs are present in the introns of coding sequences. We are now approaching a stage in which miRNA diagnostics and therapies can be established confidently, and several commercial efforts are underway to carry these innovations from the bench to the clinic. MiRNAs control many of the significant cellular a...
Source: Immunologic Research - October 29, 2021 Category: Allergy & Immunology Source Type: research

Serum levels and genetic variation of IL-35 are associated with multiple sclerosis: a population-based case –control study
This study aimed to investigate the association between serum levels and polymorphic variants of IL-35 with susceptibility, clinical features, and disease severity in multiple sclerosis (MS) patients.This case –control study recruited 186 MS patients and 195 sex- and age-matched healthy controls. Serum levels and polymorphic variants of IL-35 were determined by ELISA and restriction fragment length polymorphism (RFLP)-PCR or high resolution melting (HRM) analysis methods, respectively. In addition, by i n silico analysis, we evaluated the location and function of the polymorphism.Serum levels of IL-35 were significan...
Source: Immunologic Research - October 27, 2021 Category: Allergy & Immunology Source Type: research

Efficiency and safety of eltrombopag for multi-line failed Chinese patients with immune thrombocytopenia: cases with decreased megakaryocyte response well from single-center experience
AbstractImmune thrombocytopenia (ITP) was defined using the International Consensus Guidelines as a platelet count
Source: Immunologic Research - October 20, 2021 Category: Allergy & Immunology Source Type: research

Correction to: Primary antibody deficiencies in Turkey: molecular and clinical aspects
(Source: Immunologic Research)
Source: Immunologic Research - October 19, 2021 Category: Allergy & Immunology Source Type: research

Plummer-Vinson syndrome in primary Sj ögren syndrome: a case-based review
This study aimed to describe a patient with Sj ögren syndrome who developed Plummer-Vinson syndrome, and to review the literature and describe shared aspects of this rare association. A systematic screening of articles was conducted in PubMed/MEDLINE, LILACS, SciELO, Scopus, Web of Science, and Cochrane, dating 1940 to 2020. All the articles in cluded the association between Sjögren syndrome and Plummer-Vinson syndrome. No language restriction was applied. The following terms were used: “Sjögren syndrome” or “sicca syndrome” and “Plummer-Vinson syndrome” or “Paterson...
Source: Immunologic Research - October 14, 2021 Category: Allergy & Immunology Source Type: research

Risk of COVID-19 and its complications in patients with atopic dermatitis undergoing dupilumab treatment —a population-based cohort study
AbstractThe risk of coronavirus disease (COVID-19) infection and its complications among patients with atopic dermatitis (AD) treated by dupilumab is yet to be determined. We aimed to assess the risk of SARS-CoV-2 infection, COVID-19-associated hospitalization, and mortality among patients with AD treated by dupilumab. A population-based cohort study was conducted to compare AD patients treated by dupilumab (n = 238) with those treated by prolonged systemic corticosteroids (≥ 3 months;n = 1,023), phototherapy (n = 461), and azathioprine or mycophenolate mofetil (MMF;...
Source: Immunologic Research - October 13, 2021 Category: Allergy & Immunology Source Type: research

The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy
AbstractWhile β-hemolytic streptococcus (β-HS) infections are known to predispose patients to acute poststreptococcal glomerulonephritis, there is evidence that implicates α-hemolytic streptococcus (α-HS) in IgA nephropathy (IgAN). The alternative pathway of the complement system has also been implicated in I gAN. We aimed to explore the association between α-HS and complement activation in human tonsillar mononuclear cells (TMCs) in IgAN. In our study, α-HS induced higher IgA levels than IgG levels, while β-HS increased higher IgG levels than IgA levels with more activation-induced c...
Source: Immunologic Research - October 12, 2021 Category: Allergy & Immunology Source Type: research

Bergamottin protects against LPS-induced endotoxic shock by regulating the NF- κB signaling pathway
In this study, we investigated the protective effects and mechanisms of Bergamottin against LPS-induced inflammatory responses.Raw264.7 cells were pre-treated with Bergamottin, then stimulated with LPS. Morphologic analysis and flow cytometry were used to measure Bergamottin-related cytotoxicity. ELISA and qPCR were performed to measure secretion and transcription activities of inflammatory cytokines. Biochemical analysis was used to determine the expression of tissues damage indicators. Western blots were used to determine protein expression, and immunofluorescence staining was used to determine the co-localization of NF-...
Source: Immunologic Research - October 11, 2021 Category: Allergy & Immunology Source Type: research

How to report the antinuclear antibodies (anti-cell antibodies) test on HEp-2 cells: guidelines from the ICAP initiative
AbstractResults of the anti-nuclear antibodies-indirect immunofluorescence assay (anti-cell antibodies test) on HEp-2 cell substrates should be communicated to clinicians in a standardized way, adding value to laboratory findings and helping with critical clinical decisions. This paper proposes a test report based on the practices informed by 118 laboratories in 68 countries, with recommendations from the International Consensus on ANA Patterns (ICAP) group. Major focus is placed on the report format containing endpoint titers, immunofluorescence patterns together with anti-cell (AC) nomenclature, remarks on follow-up or r...
Source: Immunologic Research - October 9, 2021 Category: Allergy & Immunology Source Type: research

The effect of mutatio-type on proteo-phenotype and clinico-phenotype in selected primary immunodeficiencies
In this study, it was aimed to understand genotype-proteophenotype-clinicophenotype correlation by investigating the effect of mutation types on protein expression, function, and clinical severity in X-linked, autosomal dominant, and autosomal recessive forms of PIDs. It was searched in PubMed and Web of Science without any restrictions on study design and publication time. Totally, 1178 patients with PIDs who have 553 different mutations were investigated from 174 eligible full-text articles. For all mutations, the effect of mutation type on protein expressions and protein functions was analyzed. Furthermore, the most fre...
Source: Immunologic Research - October 7, 2021 Category: Allergy & Immunology Source Type: research

Primary antibody deficiencies in Turkey: molecular and clinical aspects
In this study, we describe the molecular and the clinical aspects of fifty-two PAD patients. The most common symptoms of our cohort were upper and lower respiratory infections, bronchiectasis, diarrhea, and recurrent fever. Almost all patients (98%) had at least one of the symptoms like autoimmunity, lymphoproliferation, allergy, or gastrointestinal disease. A custom-made next-generation sequencing (NGS) panel, which contains 24 genes, was designed to identify well-known disease-causing variants in our cohort. We identified eight variants (15.4%) among 52 PAD patients. The variants mapped toBTK (n = 4),CD40L ...
Source: Immunologic Research - October 7, 2021 Category: Allergy & Immunology Source Type: research

The effect of mutatio-type on proteo-phenotype and clinico-phenotype in selected primary immunodeficiencies
In this study, it was aimed to understand genotype-proteophenotype-clinicophenotype correlation by investigating the effect of mutation types on protein expression, function, and clinical severity in X-linked, autosomal dominant, and autosomal recessive forms of PIDs. It was searched in PubMed and Web of Science without any restrictions on study design and publication time. Totally, 1178 patients with PIDs who have 553 different mutations were investigated from 174 eligible full-text articles. For all mutations, the effect of mutation type on protein expressions and protein functions was analyzed. Furthermore, the most fre...
Source: Immunologic Research - October 7, 2021 Category: Allergy & Immunology Source Type: research

Primary antibody deficiencies in Turkey: molecular and clinical aspects
In this study, we describe the molecular and the clinical aspects of fifty-two PAD patients. The most common symptoms of our cohort were upper and lower respiratory infections, bronchiectasis, diarrhea, and recurrent fever. Almost all patients (98%) had at least one of the symptoms like autoimmunity, lymphoproliferation, allergy, or gastrointestinal disease. A custom-made next-generation sequencing (NGS) panel, which contains 24 genes, was designed to identify well-known disease-causing variants in our cohort. We identified eight variants (15.4%) among 52 PAD patients. The variants mapped toBTK (n = 4),CD40L ...
Source: Immunologic Research - October 7, 2021 Category: Allergy & Immunology Source Type: research

Azilsartan attenuates lipopolysaccharide-induced acute lung injury via the Nrf2/HO-1 signaling pathway
AbstractAcute lung injury(ALI) is a severe complication of sepsis and hemorrhagic shock with high morbidity. In the present study, the protective effect of Azilsartan on lipopolysaccharide (LPS)-induced ALI in mice was investigated to explore the potential therapeutic property of Azilsartan for the treatment of ALI. LPS was used to induce an ALI model in mice. Hematoxylin –eosin (HE) staining sections were then evaluated for the pathological state of lung tissues. Bronchoalveolar lavage fluid (BALF) protein concentration, wet/dry weight ratios of lung tissues, and pulmonary myeloperoxidase (MPO) activity were detecte...
Source: Immunologic Research - October 4, 2021 Category: Allergy & Immunology Source Type: research

Circular RNA hsa_circ_0001598 promotes programmed death-ligand-1-mediated immune escape and trastuzumab resistance via sponging miR-1184 in breast cancer cells
In conclusion, we demonstrate that circ_0001598/miR-1184/PD-L1 signaling plays a crucial role in the regulation of BC progression and trastuzumab-resistance phonotypes, which suggests that circ_0001598 may be a molecular target to treat HER2-positive BC patients. (Source: Immunologic Research)
Source: Immunologic Research - September 24, 2021 Category: Allergy & Immunology Source Type: research

Chimeric antigen receptor (CAR) immunotherapy: basic principles, current advances, and future prospects in neuro-oncology
AbstractWith recent advances, chimeric antigen receptor (CAR) immunotherapy has become a promising modality for patients with refractory cancer diseases. The successful results of CAR T cell therapy in relapsed and refractory B-cell malignancies shifted the paradigm of cancer immunotherapy by awakening the scientific, clinical, and commercial interest in translating this technology for the treatment of solid cancers. This review elaborates on fundamental principles of CAR T cell therapy (development of CAR construct, challenges of CAR T cell therapy) and its application on solid tumors as well as CAR T cell therapy potenti...
Source: Immunologic Research - September 23, 2021 Category: Allergy & Immunology Source Type: research

Association between TLR2 polymorphisms ( − 196–174 Ins/Del, R677W, R753Q, and P631H) and schizophrenia in a Tunisian population
AbstractSince immune dysregulation has been well studied in schizophrenia pathophysiology, recent studies showed a potent role of TLR2 in neuroinflammation process underlying schizophrenia pathogenesis. However, the genetic predisposition is still unclear. Thus, we hypothesized that TLR2 polymorphisms  − 196–174 Ins/Del (rs111200466), R753Q (rs5743708), R677W (rs121917864), and P631H (rs5743704) could be involved in schizophrenia predisposition. A case–control study was performed on a Tunisian population composed of 250 healthy controls and 250 patients genotyped by PCR–RFLP. Genotype ...
Source: Immunologic Research - September 21, 2021 Category: Allergy & Immunology Source Type: research

A graph centrality-based approach for candidate gene prediction for type 1 diabetes
AbstractType 1 diabetes mellitus (T1DM) or insulin-dependent diabetes is an autoimmune disease that may pose life-threatening situations to individuals. In most cases, cytotoxic T lymphocytes (CTLs) promotes killing of islets of Langerhans in the pancreas, which harbour insulin-producing beta cells. The trigger for autoimmune attack is still unclear; therefore, identifying and targeting candidate genes are imperative to hinder its deleterious effects. In the present study, we focused on identification of new candidate genes for T1DM. For our study, we exclusively selected immune-related genes as they play a crucial role in...
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

AKAP12/Gravin is over-expressed in patients with ulcerative colitis
AbstractThe gene of A-kinase anchor protein 12 (AKAP12) regulates cell cycle progression, cell motility, and morphology through its multiple scaffolding domains. However, the role of AKAP12 expression in ulcerative colitis (UC) patients has not been yet described. The aim of the study was to describe the gene and protein of AKAP12 expression in patients with UC and its association regarding the disease severity. We included a total of 40 patients with confirmed diagnosis of UC and 25 controls without endoscopic evidence of colitis or neoplasia. The relative quantification of the gene expression was performed by real-time P...
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

Recent advances in the role of Th17/Treg cells in tumor immunity and tumor therapy
AbstractTh17 and Treg cells play an important role in regulating tissue inflammation and maintaining the stability of the immune system. They regulate inflammatory responses, participate in the occurrence and development of autoimmune diseases and tumors, and determine the disease progress. Malignant tumor is one of the diseases with the highest mortality rate in the world. However, the efficacy of traditional treatment is limited, so it is necessary to find safe and efficient treatment methods. Studies have shown that the balance of Th17/Treg cells plays a critical role in tumor progression. In this paper, we review the a...
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

Humoral immune durability of IgG for anti-SARS-CoV-2 in the normal pregnant women in Wuhan, China
(Source: Immunologic Research)
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

Tonsillectomy and the incidence of various types of cancer
AbstractA potential connection between tonsillectomy and the development of various cancer types has repeatedly been reported in the scientific literature, but many studies have contradicted these observations. Thus, we have no clear evidence, neither to firmly support nor to refute the above-mentioned connection. Here, I suggest that the main reason for the lack of clearer evidence is that the investigations have so far mainly used incorrect sample groups. I propose that individual differences in the tonsils ’ involvement in immune reactions should be taken into account to solve this long-standing puzzle. (Source: Immunologic Research)
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

Asfotase Alfa hypersensitivity: an outpatient 8-steps desensitization protocol
(Source: Immunologic Research)
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

Tonsillectomy and the incidence of various types of cancer
AbstractA potential connection between tonsillectomy and the development of various cancer types has repeatedly been reported in the scientific literature, but many studies have contradicted these observations. Thus, we have no clear evidence, neither to firmly support nor to refute the above-mentioned connection. Here, I suggest that the main reason for the lack of clearer evidence is that the investigations have so far mainly used incorrect sample groups. I propose that individual differences in the tonsils ’ involvement in immune reactions should be taken into account to solve this long-standing puzzle. (Source: Immunologic Research)
Source: Immunologic Research - September 15, 2021 Category: Allergy & Immunology Source Type: research

Disrupted homeostasis of synovial hyaluronic acid and its associations with synovial mast cell proteases of rheumatoid arthritis patients and collagen-induced arthritis rats
This study aims to explore the correlations between HA concentration vs mast cell proteases, or matrix metalloproteinase-2/9 (MMP-2/9) and to investigate the association of MC-specific proteases with disrupted synovial HA homeostasis in rheumatoid arthritis (RA) or collagen-induced arthritis rats. The synovial fluid samples from no-RA and RA patients were collected; the collagen-induced arthritis (CIA) rat model was established; HA concentration and the activities of MC-protease and MMP-2/9 in the samples were detected, and the correlations were analyzed. In vitro interaction experiment was carried out by mixing MC-proteas...
Source: Immunologic Research - September 4, 2021 Category: Allergy & Immunology Source Type: research