Immunotherapeutic effects of intratumorally injected Zymosan-Adenovirus conjugates encoding constant active IRF3 in a melanoma mouse model
In this study, recombinant adenoviruses encoding active forms of interferon regulatory factor 3 (IRF3) were conjugated to zymosan particles to target phagocytic cells to create a pro-inflammatory immunomodulatory therapy. We determined TAM reprogramming by upregulation and downregulation of M1- and M2-associated genes, respectively, as well as cytokine and transcription factor expression in vitro. The overall shift to immune favorable conditions in the TME was suggested by metabolic, cytokine, and immune cell gene expression. Our data indicated that the zymosan:adenovirus (Zym:Ad) particle itself induced a shift from M2-li...
Source: Immunologic Research - November 23, 2022 Category: Allergy & Immunology Source Type: research
Expression of immune checkpoint molecules on adult and neonatal T-cells
AbstractTerm and especially preterm neonates are much more susceptible to serious bacterial infections than adults. But not only the susceptibility to infection is increased in neonates, but also their risk for developing post-inflammatory diseases such as bronchopulmonary dysplasia (BPD) and periventricular leukomalacia (PVL). This may be due to an impaired ability to terminate inflammation. In the study presented here, we aimed to investigate the proliferative response and the expression of immune-checkpoint molecules (ICM) and activation markers on neonatal T-cells in comparison to adult T-cells with the hypothesis that...
Source: Immunologic Research - November 23, 2022 Category: Allergy & Immunology Source Type: research
Retraction Note: Neuroprotective effects of G-CSF administration in microglia-mediated reactive T cell activation in vitro
(Source: Immunologic Research)
Source: Immunologic Research - November 18, 2022 Category: Allergy & Immunology Source Type: research
Interplay between C1-inhibitor and group IIA secreted phospholipase A2 impairs their respective function
In this study, we analyzed whether the presence of both hGIIA and C1-INH impairs their respective function on immune cells. hGIIA, but not recombinant and plasma-derived C1-INH, stimulates the production of IL-6, CXCL8, and TNF- α from peripheral blood mononuclear cells (PBMCs). PBMC activation mediated by hGIIA is blocked by RO032107A, a specific hGIIA inhibitor. Interestingly, C1-INH inhibits the hGIIA-induced production of IL-6, TNF-α, and CXCL8, while it does not affect hGIIA enzymatic activity. On the other hand, hGI IA reduces the capacity of C1-INH at inhibiting C1-esterase activity. Spectroscopic and molecular do...
Source: Immunologic Research - November 17, 2022 Category: Allergy & Immunology Source Type: research
miRNAs as the important regulators of myasthenia gravis: involvement of major cytokines and immune cells
AbstractMyasthenia gravis (MG) is a type of muscle paralysis created by immune responses against acetylcholine receptor proteins in neuromuscular synapses. This disease is characterized by muscle weakness, especially ocular weakness symptoms that could be ptosis (fall of the upper eyelid) or diplopia (double vision of a single object). Some patients also identified with speech and swallowing problems. The main goals of MG therapeutic approaches are to achieve remission, reduce symptoms, and improve life quality. Recently, other studies have revealed the potential role of various microRNAs (miRNAs) in the development of MG ...
Source: Immunologic Research - November 17, 2022 Category: Allergy & Immunology Source Type: research
Interplay between C1-inhibitor and group IIA secreted phospholipase A2 impairs their respective function
In this study, we analyzed whether the presence of both hGIIA and C1-INH impairs their respective function on immune cells. hGIIA, but not recombinant and plasma-derived C1-INH, stimulates the production of IL-6, CXCL8, and TNF- α from peripheral blood mononuclear cells (PBMCs). PBMC activation mediated by hGIIA is blocked by RO032107A, a specific hGIIA inhibitor. Interestingly, C1-INH inhibits the hGIIA-induced production of IL-6, TNF-α, and CXCL8, while it does not affect hGIIA enzymatic activity. On the other hand, hGI IA reduces the capacity of C1-INH at inhibiting C1-esterase activity. Spectroscopic and molecular do...
Source: Immunologic Research - November 17, 2022 Category: Allergy & Immunology Source Type: research
Hyper IgM in tricho-hepato-enteric syndrome due to TTC37 mutation
AbstractTricho-hepato-enteric syndrome (THES) (OMIM #222,470) is a rare autosomal recessive syndromic enteropathy whose primary manifestations are dysmorphism, intractable diarrhea, failure to thrive, hair abnormalities, liver disease, and immunodeficiency with low serum IgG concentrations. THES is caused by mutations of either Tetratricopeptide Repeat Domain 37 (TTC37) or Ski2 like RNA Helicase (SKIV2L), genes that encode two components of the human SKI complex. Here, we report a patient with a TTC37 homozygous mutation phenotypically typical for tricho-hepato-enteric syndrome in whom extremely elevated IgM with low IgG w...
Source: Immunologic Research - November 15, 2022 Category: Allergy & Immunology Source Type: research
The predictive potential of autoimmune-inflammatory syndrome induced by adjuvants (ASIA) criteria to assess the risk of adverse events and efficacy of immune checkpoint inhibitor therapy
This study aims to assess the predictive possibility of using the “ASIA questionnaire” in patients on immune checkpoint inhibitor therapy in comparison with determination of PD-L1 expression to predict the risk of irAES development and therapy efficacy. We examined patients (n = 91) being treated for solid tumors. The signs of ASIA were found in 74% (66/91), while ASIA syndrome was diagnosed in 54% of cases (49/91). No statistically significant difference in the frequency of ICI-dependent complication development regarding the presence of previous ASIA clinical manif estations, hereditary factors, sex, different tr...
Source: Immunologic Research - November 15, 2022 Category: Allergy & Immunology Source Type: research
Long-term use of broad-spectrum antibiotics affects Ly6Chi monocyte recruitment and IL-17A and IL-22 production through the gut microbiota in tumor-bearing mice treated with chemotherapy
AbstractThe protective effects of antibiotics against infection in cancer patients treated with chemotherapy remains unclear and related studies have been performed in healthy or pathogen-infected animal models. Here, we aimed to study the effects of antibiotic use on intestinal infection in tumor-bearing mice treated with chemotherapy and to determine the underlying mechanisms. Subcutaneous CT26 tumor-bearing mice were assigned to four groups: the control (Ctrl) group without any treatment, the antibiotic (ATB) group treated with a mixture of ampicillin, streptomycin, and colistin, the 5-fluorouracil (FU) group treated wi...
Source: Immunologic Research - November 15, 2022 Category: Allergy & Immunology Source Type: research
5Apal, Taql, Fokl, and Bsml polymorphisms and the susceptibility of Behcet ’s disease: an updated meta-analysis
ConclusionThe results of this meta-analysis provide evidence of the link between the four widely studied polymorphisms in the VDR gene and BD, indicating a robust estimate of the genetic risk. (Source: Immunologic Research)
Source: Immunologic Research - November 15, 2022 Category: Allergy & Immunology Source Type: research
New-onset eosinophilic granulomatosis with polyangiitis in 2 patients during treatment with IL-5 pathway inhibitors
(Source: Immunologic Research)
Source: Immunologic Research - November 15, 2022 Category: Allergy & Immunology Source Type: research
Correction to: H19 is involved in the regulation of inflammatory responses in acute gouty arthritis by targeting miR-22-3p
(Source: Immunologic Research)
Source: Immunologic Research - November 14, 2022 Category: Allergy & Immunology Source Type: research
