Immunoinformatic-guided designing of multi-epitope vaccine construct against Brucella Suis 1300
AbstractBrucella suis mediates the transmission of brucellosis in humans and animals and a significant facultative zoonotic pathogen found in livestock. It has the capacity to survive and multiply in a phagocytic environment and to acquire resistance under hostile conditions thus becoming a threat globally. Antibiotic resistance is posing a substantial public health threat, hence there is an unmet and urgent clinical need for immune-based non-antibiotic methods to treat brucellosis. Hence, we aimed to explore the whole proteome ofBrucella suis to predict antigenic proteins as a vaccine target and designed a novel chimeric ...
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
Coated recombinant target protein helps explore IL-1RAP CAR T-cell functionality in vitro
(Source: Immunologic Research)
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
ANA-specific antibodies, ANA patterns, anti-ds-DNA results, and clinical diagnosis: a laboratory and clinical audit
This study showed that testing for ANA-specific antibodies cannot be gated on ANA patterns. Also, there is a redundancy of these antibodies with various clinical diagnoses. Moreover, they are useful in making a diagnosis in ANA-negative patients as well with clinical suspicion. (Source: Immunologic Research)
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
Effect of MP-AzeFlu in IL-1 beta-induced IL-6 and proinflammatory cytokines
(Source: Immunologic Research)
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
Immunoinformatic-guided designing of multi-epitope vaccine construct against Brucella Suis 1300
AbstractBrucella suis mediates the transmission of brucellosis in humans and animals and a significant facultative zoonotic pathogen found in livestock. It has the capacity to survive and multiply in a phagocytic environment and to acquire resistance under hostile conditions thus becoming a threat globally. Antibiotic resistance is posing a substantial public health threat, hence there is an unmet and urgent clinical need for immune-based non-antibiotic methods to treat brucellosis. Hence, we aimed to explore the whole proteome ofBrucella suis to predict antigenic proteins as a vaccine target and designed a novel chimeric ...
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
Coated recombinant target protein helps explore IL-1RAP CAR T-cell functionality in vitro
(Source: Immunologic Research)
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
ANA-specific antibodies, ANA patterns, anti-ds-DNA results, and clinical diagnosis: a laboratory and clinical audit
This study showed that testing for ANA-specific antibodies cannot be gated on ANA patterns. Also, there is a redundancy of these antibodies with various clinical diagnoses. Moreover, they are useful in making a diagnosis in ANA-negative patients as well with clinical suspicion. (Source: Immunologic Research)
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
Effect of MP-AzeFlu in IL-1 beta-induced IL-6 and proinflammatory cytokines
(Source: Immunologic Research)
Source: Immunologic Research - December 2, 2022 Category: Allergy & Immunology Source Type: research
Autophagic reprogramming of bone marrow –derived macrophages
In conclusion, our approach predicted the protein targets mediating the interplay between autophagy and macrophage polarization. We suggest that autophagy reprograms macrophage polarization via CD68, arginase 1, Atg16L1-1, and Atg16L1-3. The current findings provide a foundation for the future use of macrophages in immunotherapy of different autoimmune disorders. (Source: Immunologic Research)
Source: Immunologic Research - December 1, 2022 Category: Allergy & Immunology Source Type: research
[Cuproptosis-related immune gene signature predicts clinical benefits from anti-PD-1/PD-L1 therapy in non-small-cell lung cancer
AbstractNon-small-cell lung cancer (NSCLC) remains the major cause of cancer-related death. Immune checkpoint inhibition has become the cornerstone treatment for NSCLC. Cuproptosis is a newly identified form of cell death relying on mitochondrial respiration that might play a role in shaping tumor immune microenvironment (TIME). The clinical significance of cuproptosis-related genes (CRGs) remains unclear and warrant investigation. The current study extracted RNA sequencing profiles and corresponding clinical information from six aggregated datasets from the Gene Expression Omnibus (GEO) repository as the training set, and...
Source: Immunologic Research - November 25, 2022 Category: Allergy & Immunology Source Type: research
Immunotherapeutic effects of intratumorally injected Zymosan-Adenovirus conjugates encoding constant active IRF3 in a melanoma mouse model
In this study, recombinant adenoviruses encoding active forms of interferon regulatory factor 3 (IRF3) were conjugated to zymosan particles to target phagocytic cells to create a pro-inflammatory immunomodulatory therapy. We determined TAM reprogramming by upregulation and downregulation of M1- and M2-associated genes, respectively, as well as cytokine and transcription factor expression in vitro. The overall shift to immune favorable conditions in the TME was suggested by metabolic, cytokine, and immune cell gene expression. Our data indicated that the zymosan:adenovirus (Zym:Ad) particle itself induced a shift from M2-li...
Source: Immunologic Research - November 23, 2022 Category: Allergy & Immunology Source Type: research
Expression of immune checkpoint molecules on adult and neonatal T-cells
AbstractTerm and especially preterm neonates are much more susceptible to serious bacterial infections than adults. But not only the susceptibility to infection is increased in neonates, but also their risk for developing post-inflammatory diseases such as bronchopulmonary dysplasia (BPD) and periventricular leukomalacia (PVL). This may be due to an impaired ability to terminate inflammation. In the study presented here, we aimed to investigate the proliferative response and the expression of immune-checkpoint molecules (ICM) and activation markers on neonatal T-cells in comparison to adult T-cells with the hypothesis that...
Source: Immunologic Research - November 23, 2022 Category: Allergy & Immunology Source Type: research
