Acute stress transiently activates macrophages and chemokines in cervical lymph nodes
AbstractAcute restraint stress (RS) is routinely used to study the effects of psychological and/or physiological stress. We evaluated the impact of RS on cervical lymph nodes in rats at molecular and cellular levels. Male Sprague –Dawley rats were subjected to stress by immobilization for 30, 60, and 120 min (RS30, RS60, and RS120, respectively) and compared with rats of a no-stress control (C) group. The expression of genes encoding chemokines CXCL1/CXCL2 (Cxcl1 andCxcl2) and their receptor CXCR2 (Cxcr2) was analyzed using reverse transcription-quantitative PCR (RT-qPCR) and microarray analyses. Immunohistochemistry and...
Source: Immunologic Research - February 14, 2024 Category: Allergy & Immunology Source Type: research
Chronic hyperglycemia impairs anti-microbial function of macrophages in response to Mycobacterium tuberculosis infection
AbstractDiabetes mellitus (DM) is a major risk factor for tuberculosis (TB), though the underlying mechanisms linking DM and TB remain ambiguous. Macrophages are a key player in the innate immune response and their phagocytic ability is enhanced in response to microbial infections. Upon infection or inflammation, they also repel invading pathogens by generating; reactive oxygen species (ROS), reactive nitrogen species (RNS), pro-inflammatory cytokines (IL-1 β and IL-6), and anti-inflammatory cytokines (IL-10). However, the robustness of these innate defensive capabilities of macrophages when exposed to hyperglycemia remai...
Source: Immunologic Research - February 12, 2024 Category: Allergy & Immunology Source Type: research
Rapid generation of an RBL cellular model to study proteins that cause allergenic reactions in vitro
AbstractAllergic diseases affect nearly 30% of people worldwide. There is a wide range of allergen sources, such as animal dander, food, venom, dust mites, and pollen. The skin prick test is the predominant technique used to identify allergenic sensitivity in vivo; the main problem is that it can be imprecise as many of the allergen extracts are made of mixtures of allergic and nonallergic components, making it difficult to identify the disease-eliciting allergen. An alternative to solve this problem is employing cellular models in vitro that may allow allergen identification, allergy diagnosis, and testing of novel potent...
Source: Immunologic Research - February 9, 2024 Category: Allergy & Immunology Source Type: research
Study of pathogenic T-helper cell subsets in Asian Indian patients with Takayasu arteritis
AbstractThe relapses and refractory disease are a challenge in the management of patients with Takayasu arteritis (TAK). We quantified pathogenic CD4 + memory T helper cells bearing surface markers CD161 and/or p-glycoprotein (MDR1) in patients with TAK. Peripheral blood mononuclear cells of 21 patients with TAK and 16 age-matched controls were stained with a nti-CD3, anti-CD4, anti-CD45RA, anti-CD161 and anti-p-glycoprotein antibodies and subjected to flow cytometry by FACS ARIAIII. Eighteen patients underwent follow-up immunophenotyping. Intracellular staining for interleukin-17 and interferon-γ was performed for ...
Source: Immunologic Research - February 8, 2024 Category: Allergy & Immunology Source Type: research
Blood and CSF anti-neuronal antibodies testing in psychotic syndromes: a retrospective analysis from a tertiary psychiatric hospital
AbstractA Consensus of Psychoimmunology Experts (Pollaket al., 2019) established a set of red flags and proposed diagnostic criteria for psychosis of autoimmune origin (AIP). Previous studies on AIP are limited by the scarcity of CSF analysis, preventing the valorization of blood anti-neuronal antibodies (Ab). The aims of this study are to determine the relative frequency and characterize AIP in a cohort of psychotic patients that underwent CSF workup. This work is a retrospective study in a tertiary psychiatric hospital. Clinical and paraclinical data were collected from medical records, and patients were classified accor...
Source: Immunologic Research - January 31, 2024 Category: Allergy & Immunology Source Type: research
Rituximab alleviates pediatric systemic lupus erythematosus associated refractory immune thrombocytopenia: a case-based review
We report a case of a child with SLE-associated ITP (SLE-ITP) who was successfully treated with rituximab. Rituximab is likely the most viable therapeutic option for refractory SLE-ITP. Furthermore, a comprehensive review of the relevant literature was performed and a concise overview of the pathogenesis and available treatment modalities for pediatric patients diagnosed with SLE and concurrent ITP was provided. (Source: Immunologic Research)
Source: Immunologic Research - January 27, 2024 Category: Allergy & Immunology Source Type: research
Evidence of concerning decline of COVID-19 vaccination in older persons
(Source: Immunologic Research)
Source: Immunologic Research - January 26, 2024 Category: Allergy & Immunology Source Type: research
Characteristics of splenic PD-1+ γδT cells in Plasmodium yoelii nigeriensis infection
AbstractAlthough the functions of programmed death-1 (PD-1) on αβ T cells have been extensively reported, a role for PD-1 in regulating γδT cell function is only beginning to emerge. Here, we investigated the phenotypic and functional characteristics of PD-1-expressing γδT cells, and the molecular mechanism was also explored in thePlasmodium yoelii nigeriensis (P. yoelii NSM)-infected mice. Flow cytometry and single-cell RNA sequencing (scRNA-seq) were performed. An inverse agonist of ROR α, SR3335, was used to investigate the role of RORα in regulating PD-1+γδT cells. The results indicated that γδT cells conti...
Source: Immunologic Research - January 24, 2024 Category: Allergy & Immunology Source Type: research
