Determination of α-Dicarbonyl compounds in traditional Chinese herbal medicines
In this study, we established and evaluated an HPLC-UV method and used it to measure the contents of four α-DCs including 3-deoxyglucosone (3-DG), glyoxal (GO), methylglyoxal (MGO), and diacetyl (DA) in 35 Chinese herbs after they have been derivatized with 4-nitro-1,2-phenylenediamine. The results uncover that 3-DG is the major component among the α-DCs, being detectable in all the selected herbs in concentrations ranging from 22.80 μg/g in the seeds of Alpinia katsumadai to 7032.75 μg/g in the fruit of Siraitia grosuenorii. The contents of the other three compounds are much lower than those of 3-DG, with GO being up ...
Source: Fitoterapia - March 28, 2024 Category: Biochemistry Authors: Yang Yang Hai-Ling Wang Rui-Tong Cheng Pei-Rong Zheng Hui-Peng Sun Zhi-Wen Liu Heng Yuan Xue-Yi Liu Wen-Yun Gao Heng Li Source Type: research
Network pharmacology and experimental insights into STAT3 inhibition by novel isoxazole derivatives of piperic acid in triple negative breast cancer
Fitoterapia. 2024 Mar 26:105927. doi: 10.1016/j.fitote.2024.105927. Online ahead of print.ABSTRACTSTAT3 is a crucial member within a family of seven essential transcription factors. Elevated STAT3 levels have been identified in various cancer types, notably in breast cancer (BC). Consequently, inhibiting STAT3 is recognized as a promising and effective strategy for therapeutic intervention against breast cancer. We herein synthesize a library of isoxazole (PAIs) from piperic acid [2E, 4E)-5-(2H-1,3-Benzodioxol-5-yl) penta-2,4-dienoic acid] on treatment with propargyl bromide followed by oxime under prescribed reaction cond...
Source: Fitoterapia - March 28, 2024 Category: Biochemistry Authors: G N Yatoo Basharat A Bhat None Zubaid-Ul-Khazir Mohammad Asif Sajad A Bhat Farhana Gulzar Fehmida Rashied Abdul Haleem Wani Ishfaq Ahmed Sajad Majeed Zargar Mushtaq A Mir Javid A Banday Source Type: research
Chemometric guided isolation of new triterpenoid saponins as acetylcholinesterase inhibitors from seeds of Achyranthes bidentata Blume
Fitoterapia. 2024 Mar 25:105925. doi: 10.1016/j.fitote.2024.105925. Online ahead of print.ABSTRACTAchyranthes bidentata Blume (Amaranthaceae) is an annual or perennial herb widely used as ethnomedicine in Traditional Chinese Medicine for treating fever, cold, ulcers, mensural pain, dementia, and osteoporosis. In the current study, UPLC-IM-Q-TOF-MS/MS-based chemometric approach was adopted for the tentative identification of fifty-six compounds in the extract and fractions of A.bidentata seeds. Further, the chemometric-guided isolation led to the isolation of two previously undescribed oleanane-type triterpenoid saponins, n...
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Shivani Puri Prithvi Pal Singh Prateek Singh Bora Upendra Sharma Source Type: research
Dendrobium officinale phenolic extract maintains proteostasis by regulating autophagy in a Caenorhabditis elegans model of Alzheimer's disease
In this study, we investigated protection effects of D. officinale phenolic extract (SH-F) and examined its underlying mechanisms by using transgenic Caenorhabditis elegans models. We found that treatment with SH-F (50 μg/mL) alleviated Aβ and tau protein toxicity in worms, and also reduced aggregation of polyglutamine proteins to help maintain proteostasis. RNA sequencing results showed that SH-F treatment significantly affected the proteolytic process and autophagy-lysosomal pathway. Furthermore, we confirmed that SH-F showing maintainance of proteostasis was dependent on bec-1 by qRT-PCR analysis and RNAi methods. Fin...
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Ying-Ting Yu Hong-Ru Lin Xin-Yan Chen Meng-Lu Sun Cong-Min Wei Meng-Fan Xue Yi-Huai Gao Wen-Bo Tang Chun-Yuan Zheng Shan Li Hong-Bing Wang Source Type: research
Analyzing chemical composition of Sargentodoxae caulis water extract and their hypouricemia effect in hyperuricemic mice
This study provided a data support for the clinical application of SC in the treatment of HUA.PMID:38537887 | DOI:10.1016/j.fitote.2024.105926 (Source: Fitoterapia)
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Wen-Wen Liu Hong-Jing Dong Zhe Zhang Xin-Hui Ma Shuang Liu Wei Huang Xiao Wang Source Type: research
Insights into the phytochemical composition and antioxidant potential of the Tunisian Ceratonia siliqua L
Fitoterapia. 2024 Mar 25:105919. doi: 10.1016/j.fitote.2024.105919. Online ahead of print.ABSTRACTCarob (Ceratonia siliqua L.) corresponds to an evergreen leguminous tree (Fabaceae family). The high phenolic content of numerous parts of carob has been deeply associated with several nutritional and functional benefits. The aim of this study was to investigate the physicochemical properties of ground carob pods and seeds, the effect of different extraction procedures as well a comprehensive phytochemical characterization of hydro-methanolic extracts (80/20 v/v) of pods and seeds by HPLC-DAD ESI-Q-TOF-MS/MS. Additionally, the...
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Ameni Dammak Fatma Chtourou Simon Vlad Luca Krystyna Skalicka-Wozniak Mohamed Bouaziz Source Type: research
Chemometric guided isolation of new triterpenoid saponins as acetylcholinesterase inhibitors from seeds of Achyranthes bidentata Blume
Fitoterapia. 2024 Mar 25:105925. doi: 10.1016/j.fitote.2024.105925. Online ahead of print.ABSTRACTAchyranthes bidentata Blume (Amaranthaceae) is an annual or perennial herb widely used as ethnomedicine in Traditional Chinese Medicine for treating fever, cold, ulcers, mensural pain, dementia, and osteoporosis. In the current study, UPLC-IM-Q-TOF-MS/MS-based chemometric approach was adopted for the tentative identification of fifty-six compounds in the extract and fractions of A.bidentata seeds. Further, the chemometric-guided isolation led to the isolation of two previously undescribed oleanane-type triterpenoid saponins, n...
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Shivani Puri Prithvi Pal Singh Prateek Singh Bora Upendra Sharma Source Type: research
Dendrobium officinale phenolic extract maintains proteostasis by regulating autophagy in a Caenorhabditis elegans model of Alzheimer's disease
In this study, we investigated protection effects of D. officinale phenolic extract (SH-F) and examined its underlying mechanisms by using transgenic Caenorhabditis elegans models. We found that treatment with SH-F (50 μg/mL) alleviated Aβ and tau protein toxicity in worms, and also reduced aggregation of polyglutamine proteins to help maintain proteostasis. RNA sequencing results showed that SH-F treatment significantly affected the proteolytic process and autophagy-lysosomal pathway. Furthermore, we confirmed that SH-F showing maintainance of proteostasis was dependent on bec-1 by qRT-PCR analysis and RNAi methods. Fin...
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Ying-Ting Yu Hong-Ru Lin Xin-Yan Chen Meng-Lu Sun Cong-Min Wei Meng-Fan Xue Yi-Huai Gao Wen-Bo Tang Chun-Yuan Zheng Shan Li Hong-Bing Wang Source Type: research
Analyzing chemical composition of Sargentodoxae caulis water extract and their hypouricemia effect in hyperuricemic mice
This study provided a data support for the clinical application of SC in the treatment of HUA.PMID:38537887 | DOI:10.1016/j.fitote.2024.105926 (Source: Fitoterapia)
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Wen-Wen Liu Hong-Jing Dong Zhe Zhang Xin-Hui Ma Shuang Liu Wei Huang Xiao Wang Source Type: research
Insights into the phytochemical composition and antioxidant potential of the Tunisian Ceratonia siliqua L
Fitoterapia. 2024 Mar 25:105919. doi: 10.1016/j.fitote.2024.105919. Online ahead of print.ABSTRACTCarob (Ceratonia siliqua L.) corresponds to an evergreen leguminous tree (Fabaceae family). The high phenolic content of numerous parts of carob has been deeply associated with several nutritional and functional benefits. The aim of this study was to investigate the physicochemical properties of ground carob pods and seeds, the effect of different extraction procedures as well a comprehensive phytochemical characterization of hydro-methanolic extracts (80/20 v/v) of pods and seeds by HPLC-DAD ESI-Q-TOF-MS/MS. Additionally, the...
Source: Fitoterapia - March 27, 2024 Category: Biochemistry Authors: Ameni Dammak Fatma Chtourou Simon Vlad Luca Krystyna Skalicka-Wozniak Mohamed Bouaziz Source Type: research
Biological effects and phytochemical study of the underground part of Iris scariosa Willd. ex Link extract: A new source of bioactive constituents
In this study, an ethanol extract of rhizomes of I. scariosa was prepared and tested for the cytotoxicity using the MTT assay. The extract exhibited the most potent cytotoxicity against the breast cancer cell line MCF7 (IC50 = 9.28 ± 0.49 μg/ml, selectively index ˃5), and induced apoptosis in MCF7 lines. Notably, the extract significantly inhibited the colony formation of MCF7 and HepG2 cancer cells at a concentration range from 10.6 to 85.0 μg/ml, including non-toxic concentrations for HepG2 cells. The ethanol extract was analyzed by HPLC, revealed the identification of 5 metabolites (quercetin, rutin, myricetin, apig...
Source: Fitoterapia - March 26, 2024 Category: Biochemistry Authors: Botagoz A Omarova Elvira E Shults Kairat S Zhakipbekov Аssima О Abekova Margarita Yu Ishmuratova Tatyana N Petrova Elmira B Kartbayeva Source Type: research
Biological effects and phytochemical study of the underground part of Iris scariosa Willd. ex Link extract: A new source of bioactive constituents
In this study, an ethanol extract of rhizomes of I. scariosa was prepared and tested for the cytotoxicity using the MTT assay. The extract exhibited the most potent cytotoxicity against the breast cancer cell line MCF7 (IC50 = 9.28 ± 0.49 μg/ml, selectively index ˃5), and induced apoptosis in MCF7 lines. Notably, the extract significantly inhibited the colony formation of MCF7 and HepG2 cancer cells at a concentration range from 10.6 to 85.0 μg/ml, including non-toxic concentrations for HepG2 cells. The ethanol extract was analyzed by HPLC, revealed the identification of 5 metabolites (quercetin, rutin, myricetin, apig...
Source: Fitoterapia - March 26, 2024 Category: Biochemistry Authors: Botagoz A Omarova Elvira E Shults Kairat S Zhakipbekov Аssima О Abekova Margarita Yu Ishmuratova Tatyana N Petrova Elmira B Kartbayeva Source Type: research
Bioactive solanidane steroidal alkaloids from Solanum lyratum
Fitoterapia. 2024 Mar 23:105916. doi: 10.1016/j.fitote.2024.105916. Online ahead of print.ABSTRACTSix previously unreported solanidane steroidal alkaloids, namely lyrasolanosides A-F, were isolated from Solanum lyratum. In addition, five known steroidal alkaloids were also identified. The structures of these compounds were determined through the use of NMR, HRESIMS,UV, IR and ECD analysis. To assess their bioactivities, the cytotoxic effects of the six previously unreported compounds were evaluated on A549 cells. The results revealed that lyrasolanoside B (2) exhibited the highest potency among them. Lyrasolanoside B (2) e...
Source: Fitoterapia - March 25, 2024 Category: Biochemistry Authors: Tong Wu Xiao Du Hai-Hui Liu Liang-Yu Liu Yu-Ke Yang Su-Juan Wang Chang-Ling Duan Source Type: research
Discovery of novel secondary metabolites from the basidiomycete Lentinus cf. sajor-caju and their inhibitory effects on Staphylococcus aureus biofilms
Fitoterapia. 2024 Mar 18;175:105904. doi: 10.1016/j.fitote.2024.105904. Online ahead of print.ABSTRACTThree novel derivatives of microporenic acid, microporenic acids H-J, were identified from submerged cultures of a Lentinus species obtained from a basidiome collected during a field trip in the tropical rainforest in Western Kenya. Their structures were elucidated via HR-ESIMS spectra and 1D/2D NMR spectroscopic analyses, as well as by comparison with known derivatives. Applying biofilm assays based on crystal violet staining and confocal microscopy, two of these compounds, microporenic acids H and I, demonstrated the abi...
Source: Fitoterapia - March 20, 2024 Category: Biochemistry Authors: Haoxuan Zeng Marc Stadler Cony Decock Josphat Clement Matasyoh Hedda Schrey Mathias M üsken Source Type: research
Ginkgolides with anti-PAF activity from Ginkgo biloba L
Fitoterapia. 2024 Mar 19;175:105915. doi: 10.1016/j.fitote.2024.105915. Online ahead of print.ABSTRACTFour undescribed ginkgolides, including two rare sesquiterpene ginkgolides (compounds 1 and 2) and two diterpenoid ginkgolides (compounds 3 and 4), were isolated from Ginkgo biloba L. The structures of these four ginkgolides were identified based on extensive spectroscopic analysis, DP4+ probability analysis and X-ray diffraction. Compounds 1 and 2 exhibited excellent antiplatelet aggregation activities with IC50 values of 1.20 ± 0.25 and 4.11 ± 0.34 μM, respectively.PMID:38508499 | DOI:10.1016/j.fitote.2024.105915 (Source: Fitoterapia)
Source: Fitoterapia - March 20, 2024 Category: Biochemistry Authors: Jing Zhang Sha Chen Peng Sun Yan Liu Jinzhu Jiang Cong Guo Jintang Cheng Xianju Liu Jun Zhang Chang Chen Jipeng Di An Liu Qingxia Xu Source Type: research
